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Abstract Background Bacillus subtilis is well known for promoting plant growth and reducing abiotic and biotic stresses. Mutant gene-defective models can be created to understand important traits associated with rhizosphere fitness. This study aimed to analyze the role of exopolymeric genes in modulating tomato rhizosphere microbiome assembly under a gradient of soil microbiome diversities using the B. subtilis wild-type strain UD1022 and its corresponding mutant strain UD1022eps−TasA, which is defective in exopolysaccharide (EPS) and TasA protein production. Results qPCR revealed that the B. subtilis UD1022eps−TasA− strain has a diminished capacity to colonize tomato roots in soils with diluted microbial diversity. The analysis of bacterial β-diversity revealed significant differences in bacterial and fungal community structures following inoculation with either the wild-type or mutant B. subtilis strains. The Verrucomicrobiota, Patescibacteria, and Nitrospirota phyla were more enriched with the wild-type strain inoculation than with the mutant inoculation. Co-occurrence analysis revealed that when the mutant was inoculated in tomato, the rhizosphere microbial community exhibited a lower level of modularity, fewer nodes, and fewer communities compared to communities inoculated with wild-type B. subtilis. Conclusion This study advances our understanding of the EPS and TasA genes, which are not only important for root colonization but also play a significant role in shaping rhizosphere microbiome assembly. Future research should concentrate on specific microbiome genetic traits and their implications for rhizosphere colonization, coupled with rhizosphere microbiome modulation. These efforts will be crucial for optimizing PGPR-based approaches in agriculture.

IntroductionPneumococcal conjugate vaccines (PCVs), including higher valency vaccines such as PCV20, have the potential to reduce pediatric otitis media. We assessed serotype distribution, potential PCV coverage, and antimicrobial susceptibility of Streptococcus pneumoniae isolates cultured from middle ear fluid (MEF) of US children age ≤5 years.MethodsS. pneumoniae isolates identified from US hospitals participating in the SENTRY Antimicrobial Surveillance program from 2011 to 2021 were included. Serotypes were determined by in silico analysis based on Pneumococcal Capsular Typing methodology. The percentage of isolates belonging to serotypes included in PCV13 (serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F), PCV15 (PCV13 plus 22F, 33F), and PCV20 (PCV13 plus, 8, 10A, 11A, 12F, 15B, 22F and 33F) was calculated. Antimicrobial susceptibility testing was performed by broth microdilution and interpreted using CLSI criteria. Nonsusceptibility was defined as isolates that were intermediate or resistant to a selected antimicrobial.ResultsAmong the 199 S. pneumoniae isolates that were identified, 56.8% were from children age

RESUMO Introdução: Na literatura, diversos artigos apresentam a satisfação dos pacientes e a melhor clareza de entendimento acerca das informações transmitidas pela equipe médica, com o auxílio de peças tridimensionais. A educação e a saúde são práticas inseparáveis e interdependentes, sempre estiveram articuladas, consideradas elementos fundamentais no processo de atuação dos profissionais da saúde. Assim, professores e alunos do curso de Medicina da Universidade Federal de Pernambuco criaram uma extensão universitária, que objetivava o uso de modelos anatômicos, impressos em 3D, para educação dos pacientes do ambulatório de ortopedia e traumatologia. Relato de experiência: Ao longo dos seis meses de projeto, foram assistidos 77 pacientes, e o projeto contou com o trabalho de três professores e 18 alunos da graduação, totalizando 98 pessoas envolvidas no projeto. As ações foram divididas em dois blocos. O primeiro consistiu na capacitação dos alunos. No segundo, os discentes realizavam visitas ao ambulatório, acompanhados por um médico especialista responsável, usavam peças impressas pelos próprios alunos, para orientar os pacientes quanto à sua respectiva condição, e davam orientações sobre a terapêutica valendo-se dessas peças impressas. Discussão: A possibilidade de utilização dessa ferramenta como auxílio na comunicação médica abre um vasto horizonte de aplicação da impressão 3D na educação popular em saúde. Isso, por sua vez, propicia o aperfeiçoamento da promoção da saúde de regiões menos desenvolvidas, uma vez que essa interação entre equipe de saúde e comunidade permite a promoção, a proteção e a recuperação da saúde, a partir de um diálogo horizontal, valorizando e respeitando o usuário do sistema de saúde, de maneira a torná-lo agente e protagonista do processo saúde e doença. Conclusão: Projetos de extensão desse tipo têm um enorme potencial para gerar impactos na medicina, na comunidade acadêmica e na população assistida, sobretudo a menos instruída.

Abstract Background Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen. Results The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides. Conclusion Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.

Society is becoming more demanding with aquaculture’s environmental footprint and animal wellbeing. In order to potentially mitigate these concerns, feed formulations could be based on eco-efficient (circular economy-driven) or organic ingredients. This study aimed to investigate the growth performance, feed utilization, and health status of juvenile Nile tilapia (Oreochromis niloticus) when fed with such feeds. The growth trial lasted for 8 weeks, and fish had an initial weight of 31.0 ± 0.5 g (mean ± SD). Fish were fed until visual satiation, in quadruplicate, with one of three isonitrogenous and isoenergetic experimental feeds: a commercial-like feed without fishmeal (PD), a diet based on ingredients compatible with organic certification (ORG), or a feed formulated using circular economy-driven subproducts and emergent ingredients (ECO). Fish fed ECO showed a tendency for decreased feed intake, while ORG fish significantly reduced their intake compared to those fed PD. Consequently, fish fed ECO (62.7 ± 5.4 g) exhibited almost half the growth than those fed PD (107.8 ± 6.1 g), while ORG fish almost did not increase their weight (32.7 ± 1.3 g). ECO and ORG diets had a lower digestibility for protein, lipid, and energy when compared to PD. Feed utilization of fish fed ECO or ORG was also lower than those fed PD. From the health-related genes analyzed, only glutathione reductase (gsr) showed statistically significant differences, being more expressed in fish-fed ECO than those fed PD. Thus, even when such novel formulations induced extreme effects on voluntary feed intake, their impact was noted only in fish growth, but not in robustness.

Abstract Background Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a fox-susceptible and fox-resistant common bean cultivar. Results Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Conclusion Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.

The osseodensification (OD) technique differs from conventional milling for dental implant installation in that it preserves the prepared bone and compacts it toward the apex and lateral walls of the socket, resulting in bone compaction. By enabling autografting, bone expansion, and high implant insertion torques, OD has become an increasingly popular option. The aim of this clinical case report is to demonstrate the predictability of combining OD with guided bone and tissue regeneration (GBR/GTR) techniques for bone expansion in the maxilla with reduced thickness, while avoiding other reconstructive surgeries. The report presents the treatment of a 32-year-old female patient who had cosmetic concerns regarding the anterior maxillary region. The patient was using an adhesive prosthesis with pontic on tooth 13 fixed between teeth 12 and 14. After the case was planned, it was decided that bone expansion in the region would be performed using the OD technique. The implant installation (AR Torque, 3.5×11.5 mm, Conexão®) and guided bone regeneration (GBR) were done with the assistance of L-PRF (Stick Bone, associated with L-PRF membrane). Following the osseointegration period, a provisional resin crown was fabricated, and a collagen matrix membrane (Mucoderm®) was used to increase vestibular soft tissue volume and shape the patient’s gingival profile. After a period of 120 days, the final crown was created and observed for a span of 5 years. The results showed stability of the case along with maintaining its esthetic and satisfactory function. The use of the osseodensification technique coupled with a connective tissue graft substitute has been anticipated for a long time. It has proven to be an excellent alternative to autogenous grafts.

Resumo Grande parte da literatura sobre rotinas organizacionais foca na estabilidade e nas alterações das atividades operacionais das organizações. De forma complementar, este trabalho ressalta que as rotinas organizacionais também podem exercer influência sobre os processos de tomada de decisões de investimento das empresas que operam no segmento de Exploração e Produção (E&P) da indústria de óleo e gás. Sendo assim, foi construído um modelo empírico para mostrar como as rotinas organizacionais amparam os processos de tomada de decisões estratégicas de investimento em novos ativos de capital em ambiente de riscos e incertezas no segmento. Por fim, apresentam-se os principais macroprocessos (higher-level routines) utilizados no segmento de E&P.

This study aimed to analyze the effect of a flow training program based on mindfulness applied to young and adult elite handball athletes. A quantitative, quasi-experimental, descriptive data analysis approach was carried out. The sample included 105 athletes (51 female and 54 male). The athletes were divided into two groups: (i) experimental (n = 53) and (ii) control (n = 52). The results of the repeated ANOVA indicated that the experimental group achieved significant improvements compared to the control group in decision making (n2p = 0.086), mental well-being (n2p = 0.045), dispositional flow state (n2p = 0.103), non-judgment (n2p = 0.040), refocusing (n2p = 0.052), and mindful traits in daily life (n2p = 0.058). A Pearson analysis demonstrated a positive correlation between dispositional flow state and mindfulness in sport and mental well-being. The correlation analysis also showed an inverse correlation between decision making and sense of control and mindful traits in daily life. The findings revealed that the program can be effective in decision making, mental well-being, dispositional flow state, mindfulness in sport, and mindful traits in daily lives of athletes.

Referees are crucial elements in football, and they must meet the physical and physiological demands each match poses to them. The aim is to analyse the physical and physiological demands of amateur referees in games at the regional level (4th division), examining the differences between the first and second halves and between assistant (age: 25.10 ± 4.97) and main referees (age: 25.65 ± 5.12). A total of 29 matches were analysed with GPS devices, and internal and external load metrics were analysed. Overall, main referees, due to their central role in game management, showed higher levels of physical and physiological load than assistant referees, with more high-intensity activities, greater distance covered and higher heart rate. The results also revealed that there were no differences between the halves for total distance covered for either the main or assistant referees. However, the main referees covered a greater distance in high-intensity running during the first half (p = 0.05; d = 0.389). These findings emphasise the importance of tailored training protocols to enhance performance and reduce fatigue-related errors, highlighting the significance of endurance, high-intensity running ability, and strategies to manage transient fatigue in referee preparation.

Abstract The objective of this work was to map the chromosomal regions responsible for iron accumulation in rice grains, in Southern Brazil. Eighty-one rice accessions were genotyped and phenotyped for Fe accumulation. Single nucleotide polymorphisms were mapped in the whole grain on chromosomes 1, 5, 6, and 10, from which 13 candidate genes were identified. Some of the genes, such as O s10g040680 0, seem to have a relationship with Fe homeostasis, while others are related to other metabolic processes or have an unknown function.

An ever-growing collection of commercial biostimulants is becoming available in a wide variety of forms and compositions to improve crop performance. Given the intricate nature of deciphering the underlying mechanisms of commercial products, which typically comprise various biological components, it is crucial for research in this area to have robust tools to demonstrate their effectiveness in field trials. Here, we took a multi-attribute approach to evaluating the impact of biostimulants on crop performance. First, we assessed the impact of a biostimulant on the soil and rhizosphere microbiomes associated to crops in eight reference farms, including corn (3 farms), soybean (2), cotton (2) and sugarcane (1), in different biomes and production contexts in Brazil and Paraguay. Second, we modeled a set of integrated indicators to measure crop responses to biostimulant application, including five analytical themes as follows: i) crop development and production (9 indicators), ii) soil chemistry (9), iii) soil physics (5), iv) soil biology (6) and v) plant health (10). Amplicon 16S rRNA and ITS sequencing revealed that the use of the biostimulant consistently changes the structure of bacterial and fungal communities associated with the production system for all evaluated crops. In the rhizosphere samples, the most responsive bacterial taxa to biostimulant application were Prevotella in cotton; Prauserella and Methylovirgula in corn; and Methylocapsa in sugar cane. The most responsive fungal taxa to biostimulant use were Arachnomyces in soybean and cotton; and Rhizophlyctis in corn. The proposed integrated indicators yielded highly favorable positive impact indices (averaging at 0.80), indicating that biostimulant-treated fields correlate with better plant development and crop performance. Prominent indices were observed for indicators in four themes: soil biology (average index 0.84), crop production (0.81), soil physics (compaction reduction 0.81), and chemical fertility (0.75). The multi-attribute approach employed in this study offers an effective strategy for assessing the efficacy of biostimulant products across a wide range of crops and production systems.

Background: Advances in the characterization and determination of AgNPs in different matrices, investigations involving biological matrices such as urine, plasma, sweat, and saliva still need more studies for routine application. Aim: The present study aimed to investigate silver nanoparticles (AgNPs) sizing in human matrices (blood plasma, sweat, urine, and saliva) by Single Particle Inductively Coupled Plasma Mass Spectrometry (spICP-MS). The influence of sample dilution, NPs concentration, storage time were studied. Methods: Blood, plasma, urine and sweat were collected from healthy volunteers aged 20–30 years. The samples were then diluted 1:20 (urine, saliva and plasma) and 1:10 (sweat) in an aqueous solution of AgNPs. After the dilutions, the samples were analyzed by spICP-MS. At the end of the analyzes, the samples were stored at room temperature (25 °C), and another at -5 °C to evaluate the stability of the AgNPs. Results: Higher concentrations of AgNPs in solution caused interference of matrix compatibility due to Ag+ detection by spICP-MS. Storage time up to 144 h for AgNPs 2 µg L − 1 solutions in 1:20 diluted urine and water was suitable for AgNPs detection. Otherwise, AgNPs 2 µg L − 1 did not present a clear signal-background differentiation in a 1:20 plasma diluted solution after a storage time of 144 h. Conclusion: This study provided new knowledge for monitoring of AgNPs in biological samples, helping with the new regulations proposed by Environmental and Health Agencies.

The aim of this systematic review is to collect and summarize the benefits of Pilates in the elderly population (>60 years old), within the current scientific production, assessing its contribution to Healthy Ageing (HA). We used PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analysis) to select, collect, and analyse this thematic. The methodological procedures were registered in the PROSPERO database. The main results of the studies analysed (n = 30) point to significant differences between the intervention and the control groups in dynamic balance, strength, mobility, functional capacity, risk of falling reduction, and mental and psychological health. Thus, the results showed that Pilates may be beneficial for the health of the elderly. The meta-analysis found statistical differences between means on the dynamic balance (mean difference (MD) = −0.0, 95% CI [−0.71, −0.50]; I2: 0%) and the aerobic capacity and aerobic resistance [(MD) = 38.29, 95% CI [6.82, 69.77]; I2: 0%). Thus, it is concluded that the efficacy of Pilates has been shown in various areas of HA and has proven to be affordable and safe for the majority of people, using just a mat on the floor. Future studies should focus on the analysis of the relationship between the cost and the benefit of a Pilates intervention in the elderly population, to better understand how health costs can be minimized and to contribute to a multidisciplinary and generalized HA. Pilates has practical application for the clinicians, therapists, and health professionals that work with the elderly population.

Biomonitoring programs have developed in the last decades, including the toxicity evaluation of endemic organisms in populations from polluted areas. Environmental bioindicators respond to contaminants as predictors of future damage to their population, ecosystem and human. Amphibian are considered very sensitive contamination bioindicators. Researchers in several continents have conducted ecological studies on the accumulation of potentially toxic elements in anuran tissues, especially in mining and agriculture areas. The Jaguaribe River is an important water source in northeastern Brazil, home of agriculture irrigated perimeters; but it also concerns users for increasing the risks of environmental contamination. The objective of this work was to quantify potentially toxic elements in Leptodactylus macrosternum carcasses, evaluating its potential as a contamination bioindicator of agrosystem areas in the Lower Jaguaribe River basin, Ceará, northeastern Brazil. The elements As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn were detected in the L. macrosternum carcasses' samples. There was no significant difference in the concentrations of detected metals by area status; however, analysis of variance suggested that individuals had more constant exposure to Cu, Ni, Cd and Pb in cultivated area status. The cluster analysis of chemicals indicated two optimal element groups, which proposed different compounds from diverse origins. Body index significantly influenced Mn and Pb, while sex influenced Fe and Pb accumulation. The results suggested that the L. macrosternum species is a conceivable local bioindicator for environmental potentially toxic elements. However, other tissues will be tested to improve their sensitivity and validate their capacity to be employed as a biomonitoring tool.

O trabalho proposto trata-se de uma manipula¸c˜ao matem´atica baseada em conceitos f´ısicos, com o objetivo de descrever o problema de estabilidade de um controlador autom´atico de uma m´aquina a vapor.

Objetivo: Analisar a percepção da gestão acadêmica sobre a construção curricular de um curso médico privado. Materiais e Métodos: Trata-se de uma pesquisa qualitativa, realizada com 3 gestores do curso médico de instituição privada, que responderam uma entrevista semiestruturada. Utilizou-se análise de conteúdo das entrevistas, que foram transcritas e revisadas. Resultados: A análise enfatizou o processo de organização do curso e a participação dos atores envolvidos na formação médica. O gestor destaca a estruturação do currículo aplicada na instituição, discorrendo sobre o papel da gestão acadêmica, a comunicação entre seus setores e o método de ensino, em suas características e impactos na formação acadêmica. Identificou-se a percepção dos entrevistados quanto à relação do estudante com as demandas comunitárias em saúde e com o corpo docente, reconhecendo pontos positivos e dificuldades a serem superadas na qualidade do processo ensino-aprendizagem. Considerações finais: A atuação dos gestores na construção e aplicação da estrutura curricular ancora-se na adoção de metodologia ativa para a formação médica. O método é percebido como um avanço, contribuindo para uma formação holística e contextualizada com a prática e as demandas de saúde da população. A participação dos gestores é relevante, interferindo direta ou indiretamente, na qualidade desse processo.

Wheat is one of the world’s most important crops, but its production relies heavily on agrochemical inputs which can be harmful to the environment when used excessively. It is well known that a multitude of microbes interact with eukaryotic organisms, including plants, and the sum of microbes and their functions associated with a given host is termed the microbiome. Plant-microbe interactions can be beneficial, neutral or harmful to the host plant. Over the last decade, with the development of next generation DNA sequencing technology, our understanding of the plant microbiome structure has dramatically increased. Considering that defining the wheat microbiome is key to leverage crop production in a sustainable way, here we describe how different factors drive microbiome assembly in wheat, including crop management, edaphic-environmental conditions and host selection. In addition, we highlight the benefits to take a multidisciplinary approach to define and explore the wheat core microbiome to generate solutions based on microbial (synthetic) communities or single inoculants. Advances in plant microbiome research will facilitate the development of microbial strategies to guarantee a sustainable intensification of crop production.

An imbalance in the production and detoxification of reactive oxygen species and other oxidants can disrupt all types of cellular compounds, and lead to a state of oxidative stress. Preventing this state is essential to improve growth and health in animal production. Therefore, the aim of this work was to assess if the dietary supplementation of curcumin could improve the antioxidant status and intestine structure and functionality of gilthead seabream postlarvae, with the global objective of improving fish growth performance and robustness. Two experimental diets supplemented with different doses of curcumin (LOW and HIGH diets), and a commercial diet were fed to quadruplicate groups of postlarvae, for 20 days. At the end of the feeding trial fish fed the supplemented diets significantly improved their antioxidant status compared to CTRL fed fish. LOW and HIGH fed fish presented lower protein oxidative damage (P 0.05). In conclusion, dietary curcumin supplementation was able to enhance gilthead seabream postlarvae robustness through a modulation of the oxidative status, increasing total antioxidant capacity and decreasing protein oxidative damage. This data provide evidence that curcumin can be a suitable feed additive to promote heath status and robustness of fish at early stages of development, therefore contributing for the development and sustainability of marine fish hatchery production.

Augmentative biological control (ABC) of insect pests is an environmentally sustainable alternative to synthetic insecticides. By performing BC, more than one control agent can be used for the same insect pest that is in different stages of its life cycle or for pests that simultaneously occur in the area. However, this relationship requires biosecurity for the control agents employed. The aim of this study was to evaluate the parasitism capacity of Trichogramma pretiosum Riley, 1879 and Telenomus remus Nixon, 1937 after ingestion of biological pesticides. The entomopathogens, Baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV), Bacillus thuringiensis (Bt) var. kurstaki, Bt var. aizawai, Beauveria bassiana, and Metarhizium anisopliae; the microbiological fungicide, Trichoderma harzianum, at concentrations recommended by the manufacturer; and a negative control (pure honey) were employed in this study. Further, forced ingestion was adopted, with the treatments mixed in honey and offered as food at two dilutions (one-part product: one-part honey and one-part product: nine parts honey). Each treatment consisted of 20 individual females for each parasitoid (T. pretiosum or T. remus). The following parameters were evaluated: female longevity, number of parasitized eggs, egg viability, and number of females and males to determine the sex ratio. For T. pretiosum, B. bassiana (1 × 1013 viable conidia 100 L H2O-1), and T. harzianum (5 × 1012 viable conidia 100 L H2O-1), the longevity of their females was reduced by the 1:1 mixture; however, this mixture did not interfere with other parameters and other biological pesticides compared to the respective controls of both dilutions. The biological pesticides did not negatively influence the parameters evaluated for T. remus. The tested products had low toxicity to the egg parasitoids, T. pretiosum and T. remus. Overall, more work is still required with parasitoids in other stages of development and with other exposure methods to confirm the selectivity of products for egg parasitoids to recommend its combined use in the field.

ABSTRACT Cefiderocol is a siderophore-conjugated cephalosporin with broad activity against Gram-negative (GN) bacteria, including carbapenem-resistant Enterobacterales (CRE), Pseudomonas aeruginosa, Acinetobacter spp., and Stenotrophomonas maltophilia. Cefiderocol was approved by the FDA for treatment of complicated urinary tract infection, hospital-acquired bacterial pneumonia, and ventilator-associated bacterial pneumonia and by the European Medicines Agency (EMA) for aerobic GN infections in adults with few treatment options. In this study, we analyzed the susceptibility of cefiderocol against GN clinical isolates that were collected from hospitalized patients in the United States and Europe in 2020 as part of the SENTRY Antimicrobial Surveillance Program. GN isolates, including 8,047 Enterobacterales, 2,282 P. aeruginosa, 650 Acinetobacter species, and 338 S. maltophilia isolates, were consecutively collected from patients in 66 hospitals in 19 countries. Susceptibility testing was performed using the CLSI broth microdilution method, and cefiderocol was tested in iron-depleted cation-adjusted Mueller-Hinton broth. Cefiderocol activity against resistant isolates, including CRE and extensively drug-resistant (XDR) isolates, was determined. Enterobacterales susceptibility to cefiderocol was 99.8% (CLSI), and CRE susceptibility was 98.2%. Cefiderocol was the most active antimicrobial against all P. aeruginosa isolates with MIC50/90 values of 0.12/0.5 mg/L, respectively (99.6% susceptible). A total of 256 P. aeruginosa isolates were XDR, 97.3% were susceptible to cefiderocol, and 7.4% were susceptible to meropenem. Acinetobacter susceptibility to cefiderocol was 97.7%. S. maltophilia susceptibility to cefiderocol was 100.0% (CLSI, 2021) and 97.9% (CLSI, 2022). These in vitro data suggest that cefiderocol is an important therapeutic option for the treatment of infections caused by Gram-negative pathogens, including isolates resistant to carbapenems with few therapeutic options. IMPORTANCE Cefiderocol is the first siderophore-conjugated cephalosporin approved for use in the treatment of human bacterial infections. Cefiderocol has broad-spectrum Gram-negative activity against difficult-to-treat bacterial pathogens that can cause serious infections. Our study examines the activity of cefiderocol against a large global collection of Gram-negative clinical isolates collected from hospitalized patients in 2020. In addition, we compare the activities of cefiderocol and recently approved β-lactam–β-lactamase inhibitor combinations against various antimicrobial-resistant pathogen groups including carbapenem-resistant Enterobacterales, meropenem-resistant Pseudomonas aeruginosa, and meropenem-resistant Acinetobacter spp. as well as isolates resistant to most classes of antimicrobial drugs. Cefiderocol was the most active antimicrobial tested against the isolates in this study. Our in vitro data suggest that cefiderocol may be useful for treatment of serious infections caused by drug-resistant Gram-negative organisms for patients with limited treatment options.

The great challenge of this work is to debate the financial and sports transformations that have occurred in the last few years in football and have provided its modernization and economic development. For that, the concepts of business models and their existing approaches in the literature was discussed. Thus, the main foundation of this work was present in the analysis of the business models used by football teams, allowing the investigation of the importance of this concept for the financial sustainability of the teams. From the analysis of the main European clubs, it becomes evident the importance of financial balance and the development of sports management in the current football scene. However, the increase in the financial disparity between the teams, and consequently the loss of competitiveness and unpredictability, put the sport's perpetuity at risk.

This work aimed to compare the visual patterns of under 16 (U16) athletes and professional basketball players. The sample was composed of 10 U16 (aged 15.2 ± 0.4 years; 7.1 ± 2.5 years of experience) and 10 professional (aged 27.6 ± 3.7 years; 18.4 ± 4.6 years of experience) basketball players. All athletes were males and right-handed. Each participant performed 50 jump shots from 10 different positions and 5 different angle shots (0°, 45°, 90°, 135° and 180°). Number of fixations, time of first and last fixation, total fixation duration and Quiet Eye (QE) time of all shots were analysed. Overall, results indicated that the U16 group showed greater within-group differences regarding shot positions, last fixation times, shot angles, and first and last fixation times. Additionally, the U16 group presented different visual strategies than those of professional players, with significant differences found for shooting positions and shot angles, particularly for shots performed on the left side of the field, and for QE times. In conclusion, our results add to the understanding that longer fixation times and longer QE time are associated with better jump-shot efficacy, and this relation is evident when comparing players of different age groups.

Soybean is a legume widely cultivated globally for its seeds, which are rich in oil and protein suitable for animal and human nutrition, and as a biofuel source. One of the main factors that limits production is soil salinity; currently there are an estimated 800 million hectares of agricultural land affected by salt stress worldwide. The aim of this research was to determine whether anatomical, morphological, nutritional, physiological, and biochemical parameters are negatively affected in soybean plants cultivated under different levels of salt stress. The experiment was randomized into five treatments (0, 50, 100, 150, and 200 mM Na+). Plants subjected to concentrations of 50 to 200 mM Na+ exhibited reductions in K (range 21% to 57%), Ca (range 38% to 63%), and Mg (range 20% to 41%) compared to controls (without Na+). Na+ stress progressively produced negative effects on photosynthetic machinery, gas exchange, and photosynthetic pigments, results clearly related to oxidative stress generated by the saline growth conditions. Interestingly, our study revealed that at concentrations up to 100 mM Na+ deposition of epicuticular wax occurred, the quantity and shape of the stomata changed, and the thickness of the leaf epidermis increased. Our broad-based, multidisciplinary, and comparative study proved that soybean plants suffer significant deleterious effects modulated by Na+ stress, mainly at concentrations above 100 mM Na+.

Drought stress is an alarming constraint to plant growth, development, and productivity worldwide. However, plant-associated bacteria, fungi, and viruses can enhance stress resistance and cope with the negative impacts of drought through the induction of various mechanisms, which involve plant biochemical and physiological changes. These mechanisms include osmotic adjustment, antioxidant enzyme enhancement, modification in phytohormonal levels, biofilm production, increased water and nutrient uptake as well as increased gas exchange and water use efficiency. Production of microbial volatile organic compounds (mVOCs) and induction of stress-responsive genes by microbes also play a crucial role in the acquisition of drought tolerance. This review offers a unique exploration of the role of plant-associated microorganisms—plant growth promoting rhizobacteria and mycorrhizae, viruses, and their interactions—in the plant microbiome (or phytobiome) as a whole and their modes of action that mitigate plant drought stress.

Resumo O aneurisma da aorta abdominal corresponde a uma dilatação anormal, enquanto a dissecção aórtica aguda é uma delaminação da túnica média com formação de um falso lúmen. A Tenascina-C é uma glicoproteína que pode ser encontrada em situações de lesão tecidual. Nesse sentido, este artigo pretendeu avaliar se a Tenascina-C pode auxiliar na avaliação do prognóstico do aneurisma da aorta abdominal e da dissecção aórtica aguda. Realizou-se uma revisão integrativa da literatura em que foram considerados elegíveis quatro artigos, sendo que dois associaram maiores níveis da Tenascina-C a fatores de proteção e menor risco de lesões, enquanto dois correlacionaram com prognóstico pior. Alguns autores acreditam que a Tenascina-C poderia ser um biomarcador elegível, mas esses estudos ainda são inconclusivos no que diz respeito a seu papel no desfecho clínico dos pacientes com aneurismas.

ABSTRACT: Anticarsia gemmatalis (Hübner: 1818) (Lepidoptera: Erebidae) is one of the main pests that affect soybean crops, causing defoliation. In the vegetative stages, defoliation occurs together with weeds, and in the reproductive stages with pathogens. In this sense, to maintain plant health, it is necessary to carry out the combined use of pesticides. Thus, this research determined the compatibility of the entomopathogenic virus AgMNPV with the main herbicides and fungicides used in soy at different times of the mixture. The artificial diet was immersed in the solutions of the pesticides and their mixtures and supplied to A. gemmatalis caterpillars, immediately and after one and two hours of mixing. The evaluation was performed by quantifying the number of dead caterpillars by mixing the AgMNPV virus with herbicides and fungicides, even after two hours of mixing if compatible. The observed scenarios showed a compatibility of the virus with the herbicides and fungicides, with mortality rates between 70 to 99% for A. gemmatalis.

Chrysodeixis includens Walker, 1858 (Lepidoptera: Noctuidae), commonly known as soybean looper, is one of the main pests that reduces soybean yield due to its defoliation capacity. Population outbreaks of this pest can occur in the vegetative stage of the crop, together with the occurrence of weeds or in the reproductive stage, at the same time as that of diseases. This often requires the use of pesticides against pests and weeds, or fungi at the same time. Thus, the objective of this study was to evaluate the compatibility of baculovirus ChinNPV with different synthetic chemical herbicides and fungicides used in soybean. Four bioassays were carried out, with or without the addition of ChinNPV to different herbicides and fungicides. The artificial diets were immersed in the solutions of the pesticides and their mixture and supplied to the caterpillars of C. includens, immediately and after one and two hours of mixing. The evaluation was performed by quantifying the number of dead caterpillars. The results showed that the ChinNPV baculovirus is compatible for mixing with all studied herbicides and fungicides, even after two hours of mixing. In all the studied scenarios, baculovirus mixed with chemical pesticides triggered the same mortality as sprayed with baculovirus alone, and values of over 80% mortality of C. includens have always been recorded.

Abstract Background Modern crop varieties are typically cultivated in agriculturally well-managed soils far from the centers of origin of their wild relatives. How this habitat expansion impacted plant microbiome assembly is not well understood. Results Here, we investigated if the transition from a native to an agricultural soil affected rhizobacterial community assembly of wild and modern common bean (Phaseolus vulgaris) and if this led to a depletion of rhizobacterial diversity. The impact of the bean genotype on rhizobacterial assembly was more prominent in the agricultural soil than in the native soil. Although only 113 operational taxonomic units (OTUs) out of a total of 15,925 were shared by all eight bean accessions grown in native and agricultural soils, this core microbiome represented a large fraction (25.9%) of all sequence reads. More OTUs were exclusively found in the rhizosphere of common bean in the agricultural soil as compared to the native soil and in the rhizosphere of modern bean accessions as compared to wild accessions. Co-occurrence analyses further showed a reduction in complexity of the interactions in the bean rhizosphere microbiome in the agricultural soil as compared to the native soil. Conclusions Collectively, these results suggest that habitat expansion of common bean from its native soil environment to an agricultural context had an unexpected overall positive effect on rhizobacterial diversity and led to a stronger bean genotype-dependent effect on rhizosphere microbiome assembly.

Background: The aim of this review was to collect and systematize results of studies from the last 5 years concerning the influence of the Pilates method (PM) on functional body composition (FBC). Methods: The criteria of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) were used in this review. We conducted research in three scientific databases: (i) Web of Science Core Collection, (ii) SCOPUS, and (iii) search directory of the library catalog of the Faculty of Sport and Physical Education of the University of Coimbra—EBSCO Discovery Services. We found 334 articles, covering the period between 1 January 2017 and 31 December 2021. After the selection process, we found 33 eligible articles. Results: The main results seem to point to a tendency to get a better body weight and body fat percentage (BFP), hip circumference (HC), waist, and skinfolds from the chest, abdomen, triceps, and supra-iliac in mostly female samples. Available evidence indicates that, in body composition (BC) and FBC, Pilates practice tends to be effective in reducing obesity as a multifactorial condition. Conclusions: It is concluded that there is a marked trend in the benefits of Pilates in FBC, which is in line with other systematic reviews at the BC level (which includes body weight (BW) and body mass index (BMI)) and in the reduction of the percentage of fat mass (FM). It is also concluded that there is a vast lack of studies on the male population, preventing further scientific development in this area. The limitations of this systematic review can be overcome with studies that bring together multidisciplinary aspects of FBC, better designed and methodologically more robust, which will allow more reliable analyses for the implementation of the Pilates method in terms of FBC. In addition, further studies with a male sample or mixed samples (men vs. women) could confirm the trend of no gender differences in the benefits of Pilates practice.

Leprosy is a disease with a clinical spectrum of presentations that is also manifested in diverse histological features. At one pole, lepromatous lesions (L-pole) have phagocytic foamy macrophages heavily parasitized with freely multiplying intracellular Mycobacterium leprae. At the other pole, the presence of epithelioid giant cells and granulomatous formation in tuberculoid lesions (T-pole) lead to the control of M. leprae replication and the containment of its spread. The mechanism that triggers this polarization is unknown, but macrophages are central in this process. Over the past few years, leprosy has been studied using large scale techniques to shed light on the basic pathways that, upon infection, rewire the host cellular metabolism and gene expression. M. leprae is particularly peculiar as it invades Schwann cells in the nerves, reprogramming their gene expression leading to a stem-like cell phenotype. This modulatory behavior exerted by M. leprae is also observed in skin macrophages. Here, we used live M. leprae to infect (10:1 multiplicity of infection) monocyte-derived macrophages (MDMs) for 48 h and analyzed the whole gene expression profile using microarrays. In this model, we observe an intense upregulation of genes consistent with a cellular immune response, with enriched pathways including peptide and protein secretion, leukocyte activation, inflammation, and cellular divalent inorganic cation homeostasis. Among the most differentially expressed genes (DEGs) are CCL5/RANTES and CYP27B1, and several members of the metallothionein and metalloproteinase families. This is consistent with a proinflammatory state that would resemble macrophage rewiring toward granulomatous formation observed at the T-pole. Furthermore, a comparison with a dataset retrieved from the Gene Expression Omnibus of M. leprae-infected Schwann cells (MOI 100:1) showed that the patterns among the DEGs are highly distinct, as the Schwann cells under these conditions had a scavenging and phagocytic gene profile similar to M2-like macrophages, with enriched pathways rearrangements in the cytoskeleton, lipid and cholesterol metabolism and upregulated genes including MVK, MSMO1, and LACC1/FAMIN. In summary, macrophages may have a central role in defining the paradigmatic cellular (T-pole) vs. humoral (L-pole) responses and it is likely that the multiplicity of infection and genetic polymorphisms in key genes are gearing this polarization.

Africanized honey bee populations (Apis mellifera L.) have been decreasing mainly due to the intense use of synthetic insecticides associated with pollution and climate change. To minimize these impacts on the environment and bee populations, the use of biological control agents has been intensified. These products are generally safer for non-target insects, such as bees, which are important pollinating insects. Thus, the objective of this study was to evaluate the impact of entomopathogenic nematodes on the longevity of the Africanized honey bee A. mellifera workers. Seven treatments were used: Heterorhabditis amazonensis, Heterorhabditis bacteriophora, Heterorhabditis indica, Steinernema carpocapsae, Steinernema feltiae, and Steinernema rarum, at a concentration of 40 infective juveniles per cm2 (IJs/cm²), and a control in which autoclaved distilled water was used. Two bioassays were performed: 1) spraying nematodes on the workers and 2) spraying nematodes on glass plates, in which the bees remained for two hours. Each treatment consisted of five replicates with 20 bees each. Bees were kept in cages of PVC (20 × 10 cm) covered with a voile fabric and provided pieces of cotton soaked in water and Candy paste. The cages were kept in a climatized room (27 ± 2 °C temperature, 60 ± 10% relative humidity, and 12 h photophase) and the mortality was evaluated from 12 to 240 hours. In bioassay 1, the three treatments with nematodes of the genus Steinernema reduced the longevity of the workers (103.9, 96.3, and 99.6 h) when compared to treatments with Heterorhabditis (149.7, 126.8, and 134.7 h), of which, only H. amazonensis (149.7 h) did not differ from the control (166.0 h). In bioassay 2, all treatments reduced the longevity of honey bees (155.4 to 93.9 h) in relation to the control (176.1 h). Entomopathogenic nematodes, especially Heterorhabditis, need to be tested using other methodologies and for different durations of exposure and application because in the laboratory, they were less selective to A. mellifera.

The objective of this study was to evaluate the effect of the entomopathogenic fungus Beauveria bassiana, used in large field crops, and of the pomegranate Punica granatum aqueous extract, with insecticidal potential for insect control, on the quality of Africanized Apis mellifera queens. The control sample was composed of sterile distilled water with Tween®. The treatments were incorporated into a voile fabric, covered with an acrylic plate and packed with queen rearing nucleus hive, so the worker bees could be in contact with control agents (B. bassiana and P. granatum). In the next day, frames with 30 cell cups for queen rearing with larvae were introduced to produce queens, which were monitored at moment of emergency. Measurements included live weight, wing size, abdomen size, thorax size, the time of emergence, and analysis of the breeding area size. Histological analyses of the hypopharyngeal glands of attendant worker bees and of the midgut of virgin queens were performed. There was no reduction in the weight of queens, no changes in the length, width, and height of their thoraxes, no changes in the width and length of the wing, and no changes in total length. However, B. bassiana increased the weight of the queens and reduced emergency time. The control agents did not interfere in villi height and breeding area. No differences were found in tissues when the treatments were compared with their control. P. granatum extract and the fungus B. bassiana did not interfere in the quality of Africanized A. mellifera queens.

The aim of this study was to assess the effects of three strains of Bacillus thuringiensis (Bt) on the longevity of workers of Africanized honey bee. Solutions at a concentration of 3 × 108 spores.mL-1 (dosage) were prepared for each strain of Bt (IPS 82, BR 81, and BR 147). Three bioassays were performed as follows: spraying on the bees, contact with the sprayed surface, and candy paste incorporated with Bt. The bees of the Bt bioassay were submitted to histological analysis of the mesenteron. The longevity of workers was assessed from one to 120 hours using different ranges. It was found that the bees that were exposed to the strain of Bt IPS 82, in the spraying test, exhibited a reduced longevity. In the contact test, the BR 147 strain reduced the longevity of the bees. In the food test, in turn, the three studied strains reduced the longevity of the bees as follows: Bt IPS 82: 64.5 hours; Bt BR 81: 64.5 hours; and Bt BR 147: 60.0 hours. The Bt BR 81 strain was considered the most selective of the evaluated strains on Apis mellifera, reducing the longevity of this bee only when it came into contact by the method of ingestion.

Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant–microbe interactions for the development of novel microbial inoculants.

The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections.

Objetivo: analisar os resultados da auditoria em saúde na área de órteses, próteses e materiais especiais (OPM) e cirurgias múltiplas e sequenciais (CMS), realizada pela Secretaria Estadual de Saúde do Rio Grande do Sul (SES/RS). Metodologia: estudo quantitativo descritivo, baseado em dados secundários da SES/RS, na área de OPM e CMS, referente ao período da prática de auditoria de pós-pagamento, de março de 2013 a janeiro de 2017, utilizando a abordagem estudo de caso. Resultados: foram analisadas 1.004 observações concluídas para o período; o número de processos administrativos com solicitação de devolução de recursos financeiros não teve uma tendência na série histórica analisada, resultado influenciado pela presença de processos administrativos não conclusos, em especial a partir de 2014, relativos à execução da auditoria ou até mesmo a judicialização do assunto pelas instituições auditadas. Quanto ao valor financeiro solicitado para devolução dos processos analisados, foi maior em 2014, decrescendo em 2015 e diminuiu consideravelmente nos anos 2016 e 2017. Considerando as 30 regiões de saúde do estado do Rio Grande do Sul, três instituições hospitalares tiveram destaque, a primeira com 43 processos administrativos com solicitação de devolução de recursos financeiros; a segunda com 30; e a terceira com 28. Conclusão: as ações em saúde, nessa área, podem ser qualificadas por meio de atenção especial para as regiões de saúde e instituições hospitalares que mais reincidem e tem grandes volumes financeiros glosados.

Urinary tract infections (UTIs) caused by Escherichia coli have been historically managed with oral antibiotics including the cephalosporins, fluoroquinolones and trimethoprim-sulfamethoxazole. The use of these agents is being compromised by the increase in extended spectrum β-lactamase (ESBL)-producing organisms, mostly caused by the emergence and clonal expansion of E. coli multilocus sequence typing (ST) 131. In addition, ESBL isolates show co-resistance to many of oral agents. Management of UTIs caused by ESBL and fluoroquinolone-resistant organisms is becoming increasingly challenging to treat outside of the hospital setting with clinicians having to resort to intravenous agents. The aim of this study was to assess the prevalence of ESBL phenotypes and genotypes among UTI isolates of E. coli collected in the US during 2017 as well as the impact of co-resistance to oral agents such as the fluoroquinolones and trimethoprim-sulfamethoxazole. The national prevalence of ESBL phenotypes of E. coli was 15.7% and was geographically distributed across all nine Census regions. Levofloxacin and trimethoprim-sulfamethoxazole-resistance rates were ≥ 24% among all isolates and this co-resistance phenotype was considerably higher among isolates showing an ESBL phenotype (≥ 59.2%) and carrying blaCTX-M-15 (≥ 69.5%). The agents with the highest potency against UTI isolates of E. coli, including ESBL isolates showing cross-resistance across oral agents, were the intravenous carbapenems. The results of this study indicate that new oral options with the spectrum and potency similar to the intravenous carbapenems would address a significant unmet need for the treatment of UTIs in an era of emergence and clonal expansion of ESBL isolates resistant to several classes of antimicrobial agents, including oral options.

BACKGROUND Leprosy is a chronic infectious disease caused by Mycobacterium leprae, and compromises the skin and peripheral nerves. This disease has been classified as multibacillary (MB) or paucibacillary (PB) depending on the host immune response. Genetic epidemiology studies in leprosy have shown the influence of human genetic components on the disease outcomes. OBJECTIVES We conducted an association study for IL2RA and TGFB1 genes with clinical forms of leprosy based on two case-control samples. These genes encode important molecules for the immunosuppressive activity of Treg cells and present differential expressions according to the clinical forms of leprosy. Furthermore, IL2RA is a positional candidate gene because it is located near the 10p13 chromosome region, presenting a linkage peak for PB leprosy. METHODS A total of 885 leprosy cases were included in the study; 406 cases from Rondonópolis County (start population), a hyperendemic region for leprosy in Brazil, and 479 cases from São Paulo state (replication population), which has lower epidemiological indexes for the disease. We tested 11 polymorphisms in the IL2RA gene and the missense variant rs1800470 in the TGFB1 gene. FINDINGS The AA genotype of rs2386841 in IL2RA was associated with the PB form in the start population. The AA genotype of rs1800470 in TGFB1 was associated with the MB form in the start population, and this association was confirmed for the replication population. MAIN CONCLUSIONS We demonstrated, for the first time, an association data with the PB form for a gene located on chromosome 10. In addition, we reported the association of TGFB1 gene with the MB form. Our results place these genes as candidates for validation and replication studies in leprosy polarisation.

The effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed. Fertilization regime affected the structure and composition of wheat rhizosphere bacterial communities. Acidobacteria and Planctomycetes were significantly depleted in treatments receiving inorganic N, whereas the addition of high levels of inorganic N enriched members of the phylum Bacteroidetes, especially after 10 weeks. Bacterial richness and diversity decreased with inorganic nitrogen inputs and was highest after organic treatment (FYM). In general, high levels of inorganic nitrogen fertilizers negatively affect bacterial richness and diversity, leading to a less stable bacterial community structure over time, whereas, more stable bacterial communities are provided by organic amendments. 16S rRNA gene-predicted functional structure was more affected by growth stage than by fertilizer treatment, although, some functions related to energy metabolism and metabolism of terpenoids and polyketides were enriched in samples not receiving any inorganic N, whereas inorganic N addition enriched predicted functions related to metabolism of other amino acids and carbohydrates. Understanding the impact of different fertilizers on the structure and dynamics of the rhizosphere microbiome is an important step toward developing strategies for production of crops in a sustainable way.

Resumo. Este paper tenta elucidar alguns tópicos sobre a evolução do pensamento de Adam Smith sobre a desigualdade, desde a Teoria dos Sentimentos Morais até a Riqueza das Nações. Ele também discute o conceito de justiça social bem como sua conexão com a desigualdade e ordem social. Abstract. This paper tries to highlight some topics about the evolution of Adam Smith’s thought about inequality, since Theory of Moral Sentiments, until the Wealth of Nations. It also discusses the mean of social justice as well its connection with inequality and social order.

O agente comunitário de saúde deve ter incorporado, em sua formação, conceitos que valorizam as necessidades das grávidas adolescentes. O estudo objetivou analisar o cuidado às grávidas adolescentes através da perspectiva e atuação de agentes comunitários de saúde. Trata-se de um estudo qualitativo e descritivo, com agentes que acompanhavam grávidas adolescentes. Foram realizadas cinco entrevistas semiestruturadas e acompanhadas de observações em diário de campo, de setembro a outubro de 2010, em Juazeiro-BA, segundo análise de conteúdo de Bardin. Comprovou-se que o agente enfrenta muitas dificuldades enquanto profissional de saúde pela falta de conhecimento suficiente resultante da carência de estratégias de educação permanente. O fato de residir na comunidade mostrou-se decisivo na criação de vínculos com a adolescente. Fato importante é que os sujeitos buscam realizar o cuidado mesmo com todos os obstáculos e dificuldades encontrados. ABSTRACT The community health agent must have incorporated in their training, concepts that value the needs of pregnant adolescents. The study aimed to analyze the care to pregnant teenagers through the perspective and performance of community health agents. It was a qualitative study, exploratory and descriptive, with agents that were pregnant adolescents. 05 semi-structured interviews were conducted and accompanied by observations in field journal, from September to October 2010, in Juazeiro-BA, second Bardin content analysis. Proved that the agent faces many difficulties while health professional by the lack of sufficient knowledge resulting from lack of permanent education strategies. The fact of residing of the community proved to be decisive in the creation of links with the teenager. The important fact is that the subject seeking to perform the same care with all the obstacles and difficulties encountered. RESUMEN El agente de salud comunitario debe han incorporado en su formación, conceptos que valoran las necesidades de las adolescentes embarazadas. El estudio pretende analizar la atención a las adolescentes embarazadas a través de la perspectiva y la actuación de agentes de salud comunitarios. Fue un estudio cualitativo y descriptivo, con agentes que fueron adolescentes embarazadas. 05 entrevistas semiestructuradas se llevó a cabo y acompañadas de observaciones en el diario de campo, de septiembre a octubre de 2010, en Juazeiro-BA, segundo análisis de contenido de Bardin. Demostró que el agente enfrenta a muchas dificultades al profesional de la salud por la falta de suficientes conocimientos derivados por la falta de estrategias de educación permanente. El hecho de que residentes de la comunidad resultada para ser decisiva en la creación de vínculos con el adolescente. El hecho importante es que el tema tratando de realizar el mismo cuidado con todas las dificultades y obstáculos encontrados. DOI: http://dx.doi.org/10.12957/reuerj.2015.15580

A Saúde do Trabalhador (ST) refere-se a um campo do saber que compreende as relações entre o trabalho e o processo saúde/doença de modo articulado a um corpo de práticas teóricas interdisciplinares. No contexto das possibilidades para se efetivar a ST na amplitude que exige este conceito, diferentes iniciativas de Educação Popular em Saúde se articulam com demandas do Controle Social em Saúde, permitindo novas abordagens para configurar a formação crítica e ativa de trabalhadores de saúde e movimentos sociais no mosaico das políticas de ST. Este artigo busca refletir sobre essas possibilidades a partir do Projeto de Extensão “Vidas Paralelas”. A sistematização da experiência permitiu concluir que estratégias de diálogo, organização político-social e troca de experiências de vida se apresentam como cenário significativo no compartilhamento de cultura e saúde do trabalhador, oportunizando crescimento coletivo e melhor qualidade de vida.

Através de ensaios em laboratório objetivou-se avaliar a possibilidade de aproveitamento do resíduo de porcelanato para a produção de tijolos de solo-cimento. São apresentados resultados de ensaios de laboratório, foram realizados ensaios de caracterização do solo, composição do resíduo, absorção de água e resistência a compressão. O aproveitamento do resíduo na fabricação do tijolo solo-cimento é uma alternativa sustentável preservando os recursos não renováveis.