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Your search keyword '"Rodriguez, Alberto R."' showing total 11 results
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11 results on '"Rodriguez, Alberto R."'

1. Functional sensory circuits built from neurons of two species

Author

Throesch, Benjamin T., bin Imtiaz, Muhammad Khadeesh, Muñoz-Castañeda, Rodrigo, Sakurai, Masahiro, Hartzell, Andrea L., James, Kiely N., Rodriguez, Alberto R., Martin, Greg, Lippi, Giordano, Kupriyanov, Sergey, Wu, Zhuhao, Osten, Pavel, Izpisua Belmonte, Juan Carlos, Wu, Jun, and Baldwin, Kristin K.

Published
2024
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2. B cells expressing authentic naive human VRC01-class BCRs can be recruited to germinal centers and affinity mature in multiple independent mouse models

Author

Huang, Deli, Abbott, Robert K., Havenar-Daughton, Colin, Skog, Patrick D., Al-Kolla, Rita, Groschel, Bettina, Blane, Tanya R., Menis, Sergey, Tran, Jenny Tuyet, Thinnes, Theresa C., Volpi, Sabrina A., Liguori, Alessia, Schiffner, Torben, Villegas, Sophia M., Kalyuzhniy, Oleksandr, Pintea, Mark, Voss, James E., Phelps, Nicole, Tingle, Ryan, Rodriguez, Alberto R., Martin, Greg, Kupryianov, Sergey, deCamp, Allan, Schief, William R., Nemazee, David, and Crotty, Shane

Published
2020

5. B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models

Author

Huang, Deli, primary, Abbott, Robert K., additional, Havenar-Daughton, Colin, additional, Skog, Patrick D., additional, Al-Kolla, Rita, additional, Groschel, Bettina, additional, Blane, Tanya R., additional, Menis, Sergey, additional, Tran, Jenny Tuyet, additional, Thinnes, Theresa C., additional, Volpi, Sabrina A., additional, Pintea, Mark, additional, Voss, James E, additional, Phelps, Nicole, additional, Tingle, Ryan, additional, Rodriguez, Alberto R., additional, Martin, Greg, additional, Kupryianov, Sergey, additional, Schief, William R., additional, Nemazee, David, additional, and Crotty, Shane, additional

Published
2020
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6. Comparative Quantification in Three-Dimensional Cultures reveals Epigenetic Memory and Higher Efficiency Retinogenesis in iPSCs Derived from Differentiated Rod Photoreceptors

Author

Hiler, Daniel, Chen, Xiang, Hazen, Jennifer, Kupriyanov, Sergey, Carroll, Patrick A., Qu, Chunxu, Xu, Beisi, Johnson, Dianna, Griffiths, Lyra, Frase, Sharon, Rodriguez, Alberto R., Martin, Greg, Zhang, Jiakun, Jeon, Jongrye, Fan, Yiping, Finkelstein, David, Eisenman, Robert N., Baldwin, Kristin, and Dyer, Michael A.

Subjects
Induced Pluripotent Stem Cells, Retinal Degeneration, Cell Culture Techniques, Cell Differentiation, Mice, Transgenic, DNA Methylation, Cellular Reprogramming, Article, Retina, Cell Line, Epigenesis, Genetic, Mice, Retinal Rod Photoreceptor Cells, Animals, Humans, sense organs, Embryonic Stem Cells
Abstract

Cell-based therapies to treat retinal degeneration are now being tested in clinical trials. However, it is not known whether the source of stem cells is important for the production of differentiated cells suitable for transplantation. To test this, we generated induced pluripotent stem cells (iPSCs) from murine rod photoreceptors (r-iPSCs) and scored their ability to make retinae by using a standardized quantitative protocol called STEM-RET. We discovered that r-iPSCs more efficiently produced differentiated retinae than did embryonic stem cells (ESCs) or fibroblast-derived iPSCs (f-iPSCs). Retinae derived from f-iPSCs had fewer amacrine cells and other inner nuclear layer cells. Integrated epigenetic analysis showed that DNA methylation contributes to the defects in f-iPSC retinogenesis and that rod-specific CTCF insulator protein-binding sites may promote r-iPSC retinogenesis. Together, our data suggest that the source of stem cells is important for producing retinal neurons in three-dimensional (3D) organ cultures.

Published
2015

7. CRISPR-Cas9–Mediated Modification of the NOD Mouse Genome With Ptpn22R619W Mutation Increases Autoimmune Diabetes

Author

Lin, Xiaotian, primary, Pelletier, Stephane, additional, Gingras, Sebastien, additional, Rigaud, Stephanie, additional, Maine, Christian J., additional, Marquardt, Kristi, additional, Dai, Yang D., additional, Sauer, Karsten, additional, Rodriguez, Alberto R., additional, Martin, Greg, additional, Kupriyanov, Sergey, additional, Jiang, Ling, additional, Yu, Liping, additional, Green, Douglas R., additional, and Sherman, Linda A., additional

Published
2016
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8. The Complete Genome Sequences, Unique Mutational Spectra, and Developmental Potency of Adult Neurons Revealed by Cloning

Author

Hazen, Jennifer L., primary, Faust, Gregory G., additional, Rodriguez, Alberto R., additional, Ferguson, William C., additional, Shumilina, Svetlana, additional, Clark, Royden A., additional, Boland, Michael J., additional, Martin, Greg, additional, Chubukov, Pavel, additional, Tsunemoto, Rachel K., additional, Torkamani, Ali, additional, Kupriyanov, Sergey, additional, Hall, Ira M., additional, and Baldwin, Kristin K., additional

Published
2016
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9. Quantification of Retinogenesis in 3D Cultures Reveals Epigenetic Memory and Higher Efficiency in iPSCs Derived from Rod Photoreceptors

Author

Hiler, Daniel, primary, Chen, Xiang, additional, Hazen, Jennifer, additional, Kupriyanov, Sergey, additional, Carroll, Patrick A., additional, Qu, Chunxu, additional, Xu, Beisi, additional, Johnson, Dianna, additional, Griffiths, Lyra, additional, Frase, Sharon, additional, Rodriguez, Alberto R., additional, Martin, Greg, additional, Zhang, Jiakun, additional, Jeon, Jongrye, additional, Fan, Yiping, additional, Finkelstein, David, additional, Eisenman, Robert N., additional, Baldwin, Kristin, additional, and Dyer, Michael A., additional

Published
2015
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10. CRISPR-Cas9-Mediated Modification of the NOD Mouse Genome With Ptpn22R619W Mutation Increases Autoimmune Diabetes.

Author

Xiaotian Lin, Pelletier, Stephane, Gingras, Sebastien, Rigaud, Stephanie, Maine, Christian J., Marquardt, Kristi, Dai, Yang D., Sauer, Karsten, Rodriguez, Alberto R., Martin, Greg, Kupriyanov, Sergey, Ling Jiang, Liping Yu, Green, Douglas R., Sherman, Linda A., Lin, Xiaotian, Jiang, Ling, and Yu, Liping

Subjects
ALLELES, PROTEINS, PROTEIN-tyrosine phosphatase, TYPE 1 diabetes, GENE expression, ANIMAL experimentation, DISEASE susceptibility, ESTERASES, GENETIC polymorphisms, GENOMES, MICE, GENETIC mutation, POLYMERASE chain reaction, RESEARCH funding, WESTERN immunoblotting, REVERSE transcriptase polymerase chain reaction, GENOTYPES
Abstract

An allelic variant of protein tyrosine phosphatase nonreceptor type 22 (PTPN22), PTPN22(R620W), is strongly associated with type 1 diabetes (T1D) in humans and increases the risk of T1D by two- to fourfold. The NOD mouse is a spontaneous T1D model that shares with humans many genetic pathways contributing to T1D. We hypothesized that the introduction of the murine orthologous Ptpn22(R619W) mutation to the NOD genome would enhance the spontaneous development of T1D. We microinjected CRISPR-Cas9 and a homology-directed repair template into NOD single-cell zygotes to introduce the Ptpn22(R619W) mutation to its endogenous locus. The resulting Ptpn22(R619W) mice showed increased insulin autoantibodies and earlier onset and higher penetrance of T1D. This is the first report demonstrating enhanced T1D in a mouse modeling human PTPN22(R620W) and the utility of CRISPR-Cas9 for direct genetic alternation of NOD mice. [ABSTRACT FROM AUTHOR]

Published
2016
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