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2 results on '"Rogers, Craig David"'

1. Ecological and molecular investigation of wheat bulb fly (Delia coarctata, Fallén, Diptera : Anthomyiidae) for the advancement of population monitoring and control methodologies

Author

Rogers, Craig David, Spoor, William., Evans, Andy., and French, Vernon

Subjects
632, chemical ecology, semichemical, root exudate, molecular diagnostics, dispersion, oviposition, spatial distribution
Abstract

Wheat bulb fly (WBF) (Delia coarctata, Fallén, Diptera: Anthomyiidae) is a pest of commercial importance in cereal crops. Control is dependent on organophosphates some of which are restricted in the UK, while current oviposition monitoring techniques are labour intensive and subjective. Eggs are not laid in association with a host-plant, therefore, prompt location of a suitable host is critical to the survival of the newly hatched larvae. Wheat bulb fly larvae have been shown to exhibit a positive chemotactic response to wheat and other host-plant seedlings and their root exudates. The objective of this study was to improve the control and population monitoring methodology associated with WBF, by investigating the ecology and specifically the chemical ecology of the WBF. Bioassays were used to investigate the behavioural response of WBF to known chemical constituents of host-plant exudates. Four secondary metabolites were found to be attractive while CO2 was found to alter the behaviour of larvae. Wheat bulb fly oviposition was assessed in field situations to describe egg laying spatially and through time. Geostatistical and ecological techniques were used to observe the spatial dependence and dispersion of oviposition and construct contour maps or scale-sized dot graphs of oviposition density. The traditional single line transect sampling pattern was compared against a more intensive sampling regime. Oviposition monitoring was conducted over a three year period to ascertain the time of peak egg density of this fly. A molecular based diagnostic test to assess WBF egg populations for damage forecasting was developed. A real time polymerase chain reaction (PCR) protocol was produced to estimate field populations of WBF eggs through the quantification of eggs from field samples. In addition endpoint PCR was used to identify the presence or absence of eggs from samples. This study gives the potential to advance current control methodology by providing the basis for the development of a lure and kill or confusion/disruption strategy, while offering a more accurate sampling system and a molecular diagnostic test, for improvement of the management of WBF.

Published
2012

2. The impacts of cover crop mixes on the penetration resistance model of an Oxisol under no-tillage.

Author

Moraes, Moacir Tuzzin de, Olbermann, Flavio José Rohden, Bonetti, João de Andrade, Pilegi, Luan Roberto, Costa, Marcos Vinícius Rampi, Pacheco, Vacilania, Rogers, Craig David, and Guimarães, Rachel Muylaert Locks

Subjects
*COVER crops, *SOIL matric potential, *SOIL cohesion, *NO-tillage, *SOIL moisture, *WILD oat
Abstract

Soil penetration resistance is used as an indicator of mechanical resistance to root growth into the soil matrix. However, penetration resistance measured only under wet soil conditions may not identify the effects of cover crops on soil structure. We aimed to determine the impact of individual and mixes of cover crops on soil penetration resistance as a function of water content and bulk density in an Oxisol under no-tillage in southern Brazil. Six treatments of individual and mixed cover crops were tested: (i) black oat; (ii) ryegrass; (iii) forage radish; (iv) wheat; (v) Fibrous-rooted crop mix: with 80 % fibrous-rooted and 20 % tap-rooted plants; and (vi) Tap-rooted crop mix: 40 % fibrous-rooted and 60 % tap-rooted plants. Soil cores were collected from a depth of 0–0.10 and 0.10–0.20 m and soil penetration resistance was evaluated under four soil water matric potentials (−6, −33, −100, and −500 kPa). Soil penetration resistance (Q) data for each treatment were fitted as a function of bulk density (ρ) and water content (θ) [Q=f(ρ, θ)]. The soil penetration resistance model was a non-linear equation (i.e., a double power function, with a negative exponent for water content and a positive exponent for bulk density). Under a given combination of bulk density and water content values, soil penetration resistance was higher after single crops than crop mixes, indicating a crop-mediated soil strengthening process. The cover crop mixes increased the macroporosity, resulting in an adequate soil physical quality for successional crops such as soybean, although no differences were observed for cover crops at soil water matric potentials of −60 hPa and −330 hPa when soil penetration resistance was used as a static measurement. The modelling of the soil resistance to penetration, as a function of soil bulk density and soil water content, showed that cover crops changed soil cohesion and reduced the impact of drying on soil strength when compared to the single cropping systems, indicating that soil penetration resistance should be modelled under a broad range of water contents, instead of only under a specific soil water content, to quantify the impacts of cover crops on soil strength. • Soil penetration resistance curve gave deeper insight into physical changes than single measure. • Mixed cover crops-maintained soil strength despite changes in moisture and bulk density. • Crop diversity improved soil physical quality, reducing strength for root growth. • The use of a crop with taproot system changes the soil structure and strength model. • Soil penetration resistance should be modelled under a broad range of water contents. [ABSTRACT FROM AUTHOR]

Published
2024
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