Speth, Benjamin, Rogers, Joshua P., Boonyoo, Napatsakorn, VanMeter, A.J., Baumbach, Jordan, Ott, Alina, Moore, Jerott, Cina, Tyler, Palmer, Reid, and Sandhu, Devinder
In soybean, asynaptic and desynaptic mutants lead to abnormal meiosis and fertility reduction. Several male-sterile, female-sterile mutants have been identified and studied in soybean, however, some of these mutants have not been mapped to locations on soybean chromosomes. The objectives of this study were to molecularly map five male-sterile, female-sterile genes (st2, st4, st5, st6, and st7) in soybean and compare the map locations of these genes with already mapped sterility genes. Microsatellite markers were used in bulked segregant analyses to locate all five malesterile, female-sterile genes to soybean chromosomes, and markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The st2, st4, st5, st6, and st7 genes were located on molecular linkage group (MLG) B1 (chromosome 11), MLG D1a (chromosome 01), MLG F (chromosome 13), MLG B2 (chromosome 14), and D1b (chromosome 02), respectively. The st2, st4, st5, st6, and st7 genes were flanked to 10.3 (~399 kb), 6.3 (~164 kb), 3.9 (~11.8 Mb), 11.0 (~409 kb), and 5.3 cM (~224 kb), and the flanked regions contained 57,17, 362, 52, and 17 predicted genes, respectively. Future characterization of candidate genes should facilitate identification of the male- and female-fertility genes, which may provide vital insights on structure and function of genes involved in the reproductive pathway in soybean. Key words: Glycine max, sterility, simple sequence repeat, genetic linkage map, male-sterile female-sterile. Chez le soya, des mutants asynaptiques et desynaptiques presentent des anomalies meiotiques et une reduction de la fertilite. Plusieurs mutants presentant une sterilite male et femelle ont ete identifies et etudies, mais certains de ceux-ci n'ont pas ete situes sur les chromosomes du soya. Les objectifs de ce travail etaient de realiser la cartographie de cinq genes de sterilite male et femelle (st2, st4, st5, st6 et st7) chez le soya et de comparer la position de ces genes avec celle d'autres genes de sterilite deja positionnes. Des marqueurs microsatellites ont ete employes dans le cadre d'analyses de segregants en melange afin d'assigner une position chromosomique aux cinq genes de sterilite male et femelle. Les marqueurs situes sur ces chromosomes ont ete employes sur des populations F2 afin de generer des cartes genetiques. Les genes st2, st4, st5, st6 et st7 ont ete assignes respectivement aux groupes de liaison moleculaires (MLG) B1 (chromosome 11), MLG D1a (chromosome 01), MLG F (chromosome 13), MLG B2 (chromosome 14) et MLG D1b (chromosome 2). Les genes st2, st4, st5, st6 et st7 ont ete assignes a des intervalles de 10,3 (~399 kb), 6,3 (~164 kb), 3,9 (~11,8 Mb), 11,0 (~409 kb) et 5,3 cM (~224 kb); ces intervalles contenaient respectivement 57,17, 362, 52 et 17 genes predits. Une future caracterisation de genes candidats devrait faciliter l'identification des genes de fertilite male et femelle, laquelle fournira un eclairage important sur la structure et la fonction des genes impliques dans la reproduction chez le soya. [Traduit par la Redaction] Mots-cles: Glycine max, sterilite, sequence simple repetee, carte genetique, sterilite male et femelle., Introduction In soybean, asynaptic and desynaptic mutants lead to abnormal meiosis and fertility reduction. This is caused by abnormal pairing of chromosomes during the first meiotic prophase, also known as [...]