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2. Condensed Tannins in White Clover (Trifolium repens) Foliar Tissues Expressing the Transcription Factor TaMYB14-1 Bind to Forage Protein and Reduce Ammonia and Methane Emissions in vitro

Author

Roldan, Marissa B., primary, Cousins, Greig, additional, Muetzel, Stefan, additional, Zeller, Wayne E., additional, Fraser, Karl, additional, Salminen, Juha-Pekka, additional, Blanc, Alexia, additional, Kaur, Rupinder, additional, Richardson, Kim, additional, Maher, Dorothy, additional, Jahufer, Zulfi, additional, Woodfield, Derek R., additional, Caradus, John R., additional, and Voisey, Christine R., additional

Published
2022
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5. Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repensL.) Outcrossed with High Anthocyanin Lines

Author

Roldan, Marissa B., Cousins, Greig, Fraser, Karl, Hancock, Kerry R., Collette, Vern, Demmer, Jerome, Woodfield, Derek R., Caradus, John R., Jones, Chris, and Voisey, Christine R.

Abstract

Condensed tannins (CT) are highly desirable in forage as they sequester dietary protein and reduce bloat and methane emissions in ruminants. However, the widely used forage legume white clover (Trifolium repens) only produces CTs in flowers and trichomes and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1from Trifolium arvensewas effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the “red midrib” trait; however, there was no evidence for CT accumulation in leaf sections with the “red V” anthocyanin marking. Ta-MYB14-1was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers.

Published
2020
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6. Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover ( Trifolium repens L.) Outcrossed with High Anthocyanin Lines.

Author

Roldan MB, Cousins G, Fraser K, Hancock KR, Collette V, Demmer J, Woodfield DR, Caradus JR, Jones C, and Voisey CR

Subjects
Plant Breeding, Plant Leaves chemistry, Plant Leaves genetics, Plant Leaves metabolism, Plant Proteins metabolism, Proanthocyanidins metabolism, Transcription Factors metabolism, Trifolium genetics, Trifolium metabolism, Plant Proteins genetics, Proanthocyanidins analysis, Transcription Factors genetics, Trifolium chemistry
Abstract

Condensed tannins (CT) are highly desirable in forage as they sequester dietary protein and reduce bloat and methane emissions in ruminants. However, the widely used forage legume white clover ( Trifolium repens ) only produces CTs in flowers and trichomes and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1 from Trifolium arvense was effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the "red midrib" trait; however, there was no evidence for CT accumulation in leaf sections with the "red V" anthocyanin marking. Ta-MYB14-1 was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers.

Published
2020
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