Search

Your search keyword '"Rollet, Kevin"' showing total 4 results
Start Over Author "Rollet, Kevin"
4 results on '"Rollet, Kevin"'

3. A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Author

Agence Nationale de la Recherche (France), Université de Strasbourg, Centre National de la Recherche Scientifique (France), De Wijn, Raphäel, Henning, Oliver, Roche, Jennifer, Engilberge, Sylvain, Rollet, Kevin, Fernández Millán, Pablo, Brillet, Karl, Betat, Heike, Mörl, Mario, Roussel, Alain, Girard, Eric, Mueller Dieckmann, Christoph, Fox, Gavin C., Olieric, Vincent, Gavira Gallardo, J. A., Lorber, Bernard, Sauter, Claude, Agence Nationale de la Recherche (France), Université de Strasbourg, Centre National de la Recherche Scientifique (France), De Wijn, Raphäel, Henning, Oliver, Roche, Jennifer, Engilberge, Sylvain, Rollet, Kevin, Fernández Millán, Pablo, Brillet, Karl, Betat, Heike, Mörl, Mario, Roussel, Alain, Girard, Eric, Mueller Dieckmann, Christoph, Fox, Gavin C., Olieric, Vincent, Gavira Gallardo, J. A., Lorber, Bernard, and Sauter, Claude

Abstract

Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

Published
2019

4. A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Author

de Wijn, Raphaël, primary, Hennig, Oliver, additional, Roche, Jennifer, additional, Engilberge, Sylvain, additional, Rollet, Kevin, additional, Fernandez-Millan, Pablo, additional, Brillet, Karl, additional, Betat, Heike, additional, Mörl, Mario, additional, Roussel, Alain, additional, Girard, Eric, additional, Mueller-Dieckmann, Christoph, additional, Fox, Gavin C., additional, Olieric, Vincent, additional, Gavira, José A., additional, Lorber, Bernard, additional, and Sauter, Claude, additional

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results