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27 results on '"Romecin, Paola"'

1. GPRC5C drives branched-chain amino acid metabolism in leukemogenesis

Author

Zhang, Yu Wei, Velasco-Hernandez, Talia, Mess, Julian, Lalioti, Maria-Eleni, Romero-Mulero, Mari Carmen, Obier, Nadine, Karantzelis, Nikolaos, Rettkowski, Jasmin, Schönberger, Katharina, Karabacz, Noémie, Jäcklein, Karin, Morishima, Tatsuya, Trincado, Juan Luis, Romecin, Paola, Martinez, Alba, Takizawa, Hitoshi, Shoumariyeh, Khalid, Renders, Simon, Zeiser, Robert, Pahl, Heike L., Béliveau, François, Hébert, Josée, Lehnertz, Bernhard, Sauvageau, Guy, Menendez, Pablo, and Cabezas-Wallscheid, Nina

Published
2023
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2. A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL

Author

Zanetti, Samanta Romina, Velasco-Hernandez, Talia, Gutierrez-Agüera, Francisco, Díaz, Víctor M., Romecín, Paola Alejandra, Roca-Ho, Heleia, Sánchez-Martínez, Diego, Tirado, Néstor, Baroni, Matteo Libero, Petazzi, Paolo, Torres-Ruiz, Raúl, Molina, Oscar, Bataller, Alex, Fuster, José Luis, Ballerini, Paola, Juan, Manel, Jeremias, Irmela, Bueno, Clara, and Menéndez, Pablo

Published
2022
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3. Integrative single‐cell expression and functional studies unravels a sensitization to cytarabine‐based chemotherapy through HIF pathway inhibition in AML leukemia stem cells.

Author

Velasco‐Hernandez, Talia, Trincado, Juan L., Vinyoles, Meritxell, Closa, Adria, Martínez‐Moreno, Alba, Gutiérrez‐Agüera, Francisco, Molina, Oscar, Rodríguez‐Cortez, Virginia C., Ximeno‐Parpal, Pau, Fernández‐Fuentes, Narcís, Petazzi, Paolo, Beneyto‐Calabuig, Sergi, Velten, Lars, Romecin, Paola, Casquero, Raquel, Abollo‐Jiménez, Fernando, de la Guardia, Rafael D., Lorden, Patricia, Bataller, Alex, and Lapillonne, Hélène

Published
2024
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4. P1356: CD84-TARGETED CART-CELL THERAPY: A NOVEL STRATEGY FOR THE TREATMENT OF HAEMATOLOGICAL MALIGNANCIES

Author

Pérez Amill, Lorenza, primary, Armand Ugon, Mercedes, additional, Peña Pérez, Sergio, additional, Val Casals, Maria, additional, Santos, Claudio, additional, Frigola, Gerard, additional, Ramon Álamo, José, additional, Guijarro, Francesca, additional, Minguela, Alfredo, additional, Bataller, Àlex, additional, Saldarriaga, Juan Gonzalo Correa, additional, Casanovas Albertí, Berta, additional, Uribe Herranz, Mireia, additional, Azucena Gonzalez, Europa, additional, Benitez-Ribas, Daniel, additional, Sanchez, Diego, additional, Tirado, Nestor, additional, Romecin, Paola, additional, Velasco, Talía, additional, Menendez, Pablo, additional, Martínez, Antonio, additional, Rovira Tarrats, Montserrat, additional, Esteve, Jordi, additional, Urbano Ispizua, Avaro, additional, Delgado, Julio, additional, Juan, Manel, additional, and Klein González, Nela, additional

Published
2023
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5. Robust In Vitro and In Vivo Immunosuppressive and Anti-inflammatory Properties of Inducible Caspase-9-mediated Apoptotic Mesenchymal Stromal/Stem Cell

Author

Alejandra Romecin, Paola, Vinyoles, Meritxell, Lopez-Millan, Belen, Diaz de la Guardia, Rafael, Atucha, Noemi M., Querol, Sergi, Bueno, Clara, Benitez, Raquel, Gonzalez-Rey, Elena, Delgado, Mario, Menendez, Pablo, [Alejandra Romecin, Paola] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Vinyoles, Meritxell] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Lopez-Millan, Belen] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Bueno, Clara] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Menendez, Pablo] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Alejandra Romecin, Paola] ISCIII, RICORS TERAV, Madrid, Spain, [Lopez-Millan, Belen] ISCIII, RICORS TERAV, Madrid, Spain, [Querol, Sergi] ISCIII, RICORS TERAV, Madrid, Spain, [Bueno, Clara] ISCIII, RICORS TERAV, Madrid, Spain, [Menendez, Pablo] ISCIII, RICORS TERAV, Madrid, Spain, [Lopez-Millan, Belen] Univ Granada, GENYO, Ctr Pfizer, Junta Andalucia Genom & Invest Oncol, Granada, Spain, [Diaz de la Guardia, Rafael] Univ Granada, GENYO, Ctr Pfizer, Junta Andalucia Genom & Invest Oncol, Granada, Spain, [Atucha, Noemi M.] Fac Med, Dept Fisiol Humana, Murcia, Spain, [Querol, Sergi] Banc Sang & Teixits, Barcelona, Spain, [Bueno, Clara] ISCIII, CIBERONC, Barcelona, Spain, [Menendez, Pablo] ISCIII, CIBERONC, Barcelona, Spain, [Benitez, Raquel] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Gonzalez-Rey, Elena] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Delgado, Mario] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Menendez, Pablo] Inst Catalana Recerca & Estudis Avancats ICREA, Barcelona, Spain, Health Canada, Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation, and Spanish Association of Cancer Research (AECC)

Subjects
iCasp9 switch, Protein, Anti-Inflammatory Agents, Mesenchymal Stem Cells, Cell Biology, General Medicine, Safety switch, BM-MSC, Experimental colitis, Caspase 9, Immunomodulation, Extracellular Vesicles, Colitis in vivo model, Improve, Humans, WJ-MSC, Anti-inflammatory, Immunosuppressive Agents, Immunosuppression, Developmental Biology
Abstract

The financial support for this work was obtained from Health Canada (H4080-144541) to P.M. M.V. and B.L.-M. were supported by a Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation (IJCI-2017-3317) and a fellowship from the Spanish Association of Cancer Research (AECC), respectively., Mesenchymal stromal stem/cells (MSC) therapies are clinically used in a wide range of disorders based on their robust HLA-independent immunosuppressive and anti-inflammatory properties. However, the mechanisms underlying MSC therapeutic activity remain elusive as demonstrated by the unpredictable therapeutic efficacy of MSC infusions reported in multiple clinical trials. A seminal recent study showed that infused MSCs are actively induced to undergo apoptosis by recipient cytotoxic T cells, a mechanism that triggers in vivo recipient-induced immunomodulation by such apoptotic MSCs, and the need for such recipient cytotoxic cell activity could be replaced by the administration of ex vivo-generated apoptotic MSCs. Moreover, the use of MSC-derived extracellular vesicles (MSC-EVs) is being actively explored as a cellfree therapeutic alternative over the parental MSCs. We hypothesized that the introduction of a “suicide gene” switch into MSCs may offer on-demand in vivo apoptosis of transplanted MSCs. Here, we prompted to investigate the utility of the iCasp9/AP1903 suicide gene system in inducing apoptosis of MSCs. iCasp9/AP1903-induced apoptotic MSCs (MSCiCasp9+) were tested in vitro and in in vivo models of acute colitis. Our data show a very similar and robust immunosuppressive and anti-inflammatory properties of both “parental” alive MSCGFP+ cells and apoptotic MSCiCasp9+ cells in vitro and in vivo regardless of whether apoptosis was induced in vivo or in vitro before administering MSCiCasp9+ lysates. This development of an efficient iCasp9 switch may potentiate the safety of MSC-based therapies in the case of an adverse event and, will also circumvent current logistic technical limitations and biological uncertainties associated to MSC-EVs., Health Canada H4080-144541, Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation IJCI-2017-3317, Spanish Association of Cancer Research (AECC)

Published
2022

6. Supplementary Figures Legends from Transcriptional Induction of Periostin by a Sulfatase 2–TGFβ1–SMAD Signaling Axis Mediates Tumor Angiogenesis in Hepatocellular Carcinoma

Author

Chen, Gang, primary, Nakamura, Ikuo, primary, Dhanasekaran, Renumathy, primary, Iguchi, Eriko, primary, Tolosa, Ezequiel J., primary, Romecin, Paola A., primary, Vera, Renzo E., primary, Almada, Luciana L., primary, Miamen, Alexander G., primary, Chaiteerakij, Roongruedee, primary, Zhou, Mengtao, primary, Asiedu, Michael K., primary, Moser, Catherine D., primary, Han, Shaoshan, primary, Hu, Chunling, primary, Banini, Bubu A., primary, Oseini, Abdul M., primary, Chen, Yichun, primary, Fang, Yong, primary, Yang, Dongye, primary, Shaleh, Hassan M., primary, Wang, Shaoqing, primary, Wu, Dehai, primary, Song, Tao, primary, Lee, Ju-Seog, primary, Thorgeirsson, Snorri S., primary, Chevet, Eric, primary, Shah, Vijay H., primary, Fernandez-Zapico, Martin E., primary, and Roberts, Lewis R., primary

Published
2023
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7. Data from Transcriptional Induction of Periostin by a Sulfatase 2–TGFβ1–SMAD Signaling Axis Mediates Tumor Angiogenesis in Hepatocellular Carcinoma

Author

Chen, Gang, primary, Nakamura, Ikuo, primary, Dhanasekaran, Renumathy, primary, Iguchi, Eriko, primary, Tolosa, Ezequiel J., primary, Romecin, Paola A., primary, Vera, Renzo E., primary, Almada, Luciana L., primary, Miamen, Alexander G., primary, Chaiteerakij, Roongruedee, primary, Zhou, Mengtao, primary, Asiedu, Michael K., primary, Moser, Catherine D., primary, Han, Shaoshan, primary, Hu, Chunling, primary, Banini, Bubu A., primary, Oseini, Abdul M., primary, Chen, Yichun, primary, Fang, Yong, primary, Yang, Dongye, primary, Shaleh, Hassan M., primary, Wang, Shaoqing, primary, Wu, Dehai, primary, Song, Tao, primary, Lee, Ju-Seog, primary, Thorgeirsson, Snorri S., primary, Chevet, Eric, primary, Shah, Vijay H., primary, Fernandez-Zapico, Martin E., primary, and Roberts, Lewis R., primary

Published
2023
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8. Supplementary Tables from Transcriptional Induction of Periostin by a Sulfatase 2–TGFβ1–SMAD Signaling Axis Mediates Tumor Angiogenesis in Hepatocellular Carcinoma

Author

Chen, Gang, primary, Nakamura, Ikuo, primary, Dhanasekaran, Renumathy, primary, Iguchi, Eriko, primary, Tolosa, Ezequiel J., primary, Romecin, Paola A., primary, Vera, Renzo E., primary, Almada, Luciana L., primary, Miamen, Alexander G., primary, Chaiteerakij, Roongruedee, primary, Zhou, Mengtao, primary, Asiedu, Michael K., primary, Moser, Catherine D., primary, Han, Shaoshan, primary, Hu, Chunling, primary, Banini, Bubu A., primary, Oseini, Abdul M., primary, Chen, Yichun, primary, Fang, Yong, primary, Yang, Dongye, primary, Shaleh, Hassan M., primary, Wang, Shaoqing, primary, Wu, Dehai, primary, Song, Tao, primary, Lee, Ju-Seog, primary, Thorgeirsson, Snorri S., primary, Chevet, Eric, primary, Shah, Vijay H., primary, Fernandez-Zapico, Martin E., primary, and Roberts, Lewis R., primary

Published
2023
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9. Supplementary Figures 1-5 from Transcriptional Induction of Periostin by a Sulfatase 2–TGFβ1–SMAD Signaling Axis Mediates Tumor Angiogenesis in Hepatocellular Carcinoma

Author

Chen, Gang, primary, Nakamura, Ikuo, primary, Dhanasekaran, Renumathy, primary, Iguchi, Eriko, primary, Tolosa, Ezequiel J., primary, Romecin, Paola A., primary, Vera, Renzo E., primary, Almada, Luciana L., primary, Miamen, Alexander G., primary, Chaiteerakij, Roongruedee, primary, Zhou, Mengtao, primary, Asiedu, Michael K., primary, Moser, Catherine D., primary, Han, Shaoshan, primary, Hu, Chunling, primary, Banini, Bubu A., primary, Oseini, Abdul M., primary, Chen, Yichun, primary, Fang, Yong, primary, Yang, Dongye, primary, Shaleh, Hassan M., primary, Wang, Shaoqing, primary, Wu, Dehai, primary, Song, Tao, primary, Lee, Ju-Seog, primary, Thorgeirsson, Snorri S., primary, Chevet, Eric, primary, Shah, Vijay H., primary, Fernandez-Zapico, Martin E., primary, and Roberts, Lewis R., primary

Published
2023
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10. ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma

Author

Norwegian Research Council, Southern and Eastern Norway Regional Health Authority, Nova Southeastern University, Swedish Society for Medical Research, Fundación la Caixa, European Commission, Instituto de Salud Carlos III, European Institute of Innovation and Technology, Josep Carreras Leukemia Foundation, Centres de Recerca de Catalunya, Generalitat de Catalunya, Mensali, Nadia, Köksal, Hakan, Joaquina, Sandy, Wernhoff, Patrik, Casey, Nicholas P., Romecin, Paola, Panisello, Carla, Rodriguez, René, Vimeux, Lene, Juzeniene, Asta, Myhre, Marit R., Fåne, Anne, Castilla, Carolina, Maggadottir, Solrun Melkorka, Duru, Adil Doganay, Georgoudaki, Anna-Maria, Grad, Iwona, Maturana, Andrés Daniel, Gaudernack, Gustav, Kvalheim, Gunnar, Carcaboso, Ángel M., Álava, Enrique de, Donnadieu, Emmanuel, Bruland, Øyvind S., Menéndez, Pablo, Inderberg, Else Marit, Wälchli, Sébastien, Norwegian Research Council, Southern and Eastern Norway Regional Health Authority, Nova Southeastern University, Swedish Society for Medical Research, Fundación la Caixa, European Commission, Instituto de Salud Carlos III, European Institute of Innovation and Technology, Josep Carreras Leukemia Foundation, Centres de Recerca de Catalunya, Generalitat de Catalunya, Mensali, Nadia, Köksal, Hakan, Joaquina, Sandy, Wernhoff, Patrik, Casey, Nicholas P., Romecin, Paola, Panisello, Carla, Rodriguez, René, Vimeux, Lene, Juzeniene, Asta, Myhre, Marit R., Fåne, Anne, Castilla, Carolina, Maggadottir, Solrun Melkorka, Duru, Adil Doganay, Georgoudaki, Anna-Maria, Grad, Iwona, Maturana, Andrés Daniel, Gaudernack, Gustav, Kvalheim, Gunnar, Carcaboso, Ángel M., Álava, Enrique de, Donnadieu, Emmanuel, Bruland, Øyvind S., Menéndez, Pablo, Inderberg, Else Marit, and Wälchli, Sébastien

Abstract

Osteosarcoma (OS) remains a dismal malignancy in children and young adults, with poor outcome for metastatic and recurrent disease. Immunotherapies in OS are not as promising as in some other cancer types due to intra-tumor heterogeneity and considerable off-target expression of the potentially targetable proteins. Here we show that chimeric antigen receptor (CAR) T cells could successfully target an isoform of alkaline phosphatase, ALPL-1, which is highly and specifically expressed in primary and metastatic OS. The target recognition element of the second-generation CAR construct is based on two antibodies, previously shown to react against OS. T cells transduced with these CAR constructs mediate efficient and effective cytotoxicity against ALPL-positive cells in in vitro settings and in state-of-the-art in vivo orthotopic models of primary and metastatic OS, without unexpected toxicities against hematopoietic stem cells or healthy tissues. In summary, CAR-T cells targeting ALPL-1 show efficiency and specificity in treating OS in preclinical models, paving the path for clinical translation.

Published
2023

11. Supplementary information. ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma

Author

Mensali, Nadia, Köksal, Hakan, Joaquina, Sandy, Wernhoff, Patrik, Casey, Nicholas P., Romecin, Paola, Panisello, Carla, Rodriguez, René, Vimeux, Lene, Juzeniene, Asta, Myhre, Marit R., Fåne, Anne, Castilla, Carolina, Maggadottir, Solrun Melkorka, Duru, Adil Doganay, Georgoudaki, Anna-Maria, Grad, Iwona, Maturana, Andrés Daniel, Gaudernack, Gustav, Kvalheim, Gunnar, Carcaboso, Ángel M., Álava, Enrique de, Donnadieu, Emmanuel, Bruland, Øyvind S., Menéndez, Pablo, Inderberg, Else Marit, Wälchli, Sébastien, Mensali, Nadia, Köksal, Hakan, Joaquina, Sandy, Wernhoff, Patrik, Casey, Nicholas P., Romecin, Paola, Panisello, Carla, Rodriguez, René, Vimeux, Lene, Juzeniene, Asta, Myhre, Marit R., Fåne, Anne, Castilla, Carolina, Maggadottir, Solrun Melkorka, Duru, Adil Doganay, Georgoudaki, Anna-Maria, Grad, Iwona, Maturana, Andrés Daniel, Gaudernack, Gustav, Kvalheim, Gunnar, Carcaboso, Ángel M., Álava, Enrique de, Donnadieu, Emmanuel, Bruland, Øyvind S., Menéndez, Pablo, Inderberg, Else Marit, and Wälchli, Sébastien

Published
2023

12. TIM3, a human acute myeloid leukemia stem cell marker, does not enrich for leukemia-initiating stem cells in B-cell acute lymphoblastic leukemia

Author

Bueno, Clara, primary, Martínez, Alba, additional, Romecin, Paola Alejandra, additional, Zanetti, Samanta Romina, additional, Tirtakusuma, Ricky, additional, Genesca, Eulalia, additional, Camós, Mireia, additional, Ramírez-Orellana, Manuel, additional, Anguita, Eduardo, additional, Ballerini, Paola, additional, Locatelli, Franco, additional, Fuster, José Luis, additional, and Menéndez, Pablo, additional

Published
2023
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13. ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma.

Author

Mensali, Nadia, Köksal, Hakan, Joaquina, Sandy, Wernhoff, Patrik, Casey, Nicholas P., Romecin, Paola, Panisello, Carla, Rodriguez, René, Vimeux, Lene, Juzeniene, Asta, Myhre, Marit R., Fåne, Anne, Ramírez, Carolina Castilla, Maggadottir, Solrun Melkorka, Duru, Adil Doganay, Georgoudaki, Anna-Maria, Grad, Iwona, Maturana, Andrés Daniel, Gaudernack, Gustav, and Kvalheim, Gunnar

Subjects
CHIMERIC antigen receptors, HEMATOPOIETIC stem cells, OSTEOSARCOMA, ALKALINE phosphatase, YOUNG adults, T cell receptors
Abstract

Osteosarcoma (OS) remains a dismal malignancy in children and young adults, with poor outcome for metastatic and recurrent disease. Immunotherapies in OS are not as promising as in some other cancer types due to intra-tumor heterogeneity and considerable off-target expression of the potentially targetable proteins. Here we show that chimeric antigen receptor (CAR) T cells could successfully target an isoform of alkaline phosphatase, ALPL-1, which is highly and specifically expressed in primary and metastatic OS. The target recognition element of the second-generation CAR construct is based on two antibodies, previously shown to react against OS. T cells transduced with these CAR constructs mediate efficient and effective cytotoxicity against ALPL-positive cells in in vitro settings and in state-of-the-art in vivo orthotopic models of primary and metastatic OS, without unexpected toxicities against hematopoietic stem cells or healthy tissues. In summary, CAR-T cells targeting ALPL-1 show efficiency and specificity in treating OS in preclinical models, paving the path for clinical translation. Chimeric antigen receptor T (CAR-T) cells represent a breakthrough in the treatment of haematopoietic malignancies, however, in solid tumours this form of immune therapy is hampered by the scarcity of suitable targets showing high level tumour-restricted expression. Here authors generate CAR-T cells that target an osteosarcoma-specific isoform of alkaline phosphatase and show efficacy in orthotopic animal models while sparing healthy tissues. [ABSTRACT FROM AUTHOR]

Published
2023
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14. CD84: A Novel Target for CAR T-Cell Therapy for Acute Myeloid Leukemia

Author

Pérez-Amill, Lorena, Armand-Ugon, Mercedes, Peña, Sergio, Val Casals, Maria, Santos, Claudio, Frigola, Gerard, Minguela, Alfredo, Bataller Torralba, Àlex, Casanovas, Berta, Álamo, José, Uribe, Mireia, Sánchez-Martínez, Diego, Tirado, Néstor, Bueno, Clara, Romecín, Paola Alejandra, Menéndez, Pablo, Martínez, Antonio, Rovira, Montserrat, Esteve, Jordi, Urbano-Ispizua, Álvaro, Delgado, Julio, Juan, Manel, and Klein-González, Nela

Published
2022
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15. Robust In Vitro and In Vivo Immunosuppressive and Anti-inflammatory Properties of Inducible Caspase-9-mediated Apoptotic Mesenchymal Stromal/Stem Cell.

Author

Ministerio de Ciencia e Innovación (España), Asociación Española Contra el Cáncer, Health Canada, Romecin, Paola, Vinyoles, Meritxell, López-Millán, Belén, Díaz de la Guardia, Rafael, Atucha, Noemi, Querol, Sergio, Bueno, Clara, Benitez, Raquel, González-Rey, Elena, Delgado, Mario, Menéndez, Pablo, Ministerio de Ciencia e Innovación (España), Asociación Española Contra el Cáncer, Health Canada, Romecin, Paola, Vinyoles, Meritxell, López-Millán, Belén, Díaz de la Guardia, Rafael, Atucha, Noemi, Querol, Sergio, Bueno, Clara, Benitez, Raquel, González-Rey, Elena, Delgado, Mario, and Menéndez, Pablo

Abstract

Mesenchymal stromal stem/cells (MSC) therapies are clinically used in a wide range of disorders based on their robust HLA-independent immunosuppressive and anti-inflammatory properties. However, the mechanisms underlying MSC therapeutic activity remain elusive as demonstrated by the unpredictable therapeutic efficacy of MSC infusions reported in multiple clinical trials. A seminal recent study showed that infused MSCs are actively induced to undergo apoptosis by recipient cytotoxic T cells, a mechanism that triggers in vivo recipient-induced immunomodulation by such apoptotic MSCs, and the need for such recipient cytotoxic cell activity could be replaced by the administration of ex vivo-generated apoptotic MSCs. Moreover, the use of MSC-derived extracellular vesicles (MSC-EVs) is being actively explored as a cell-free therapeutic alternative over the parental MSCs. We hypothesized that the introduction of a "suicide gene" switch into MSCs may offer on-demand in vivo apoptosis of transplanted MSCs. Here, we prompted to investigate the utility of the iCasp9/AP1903 suicide gene system in inducing apoptosis of MSCs. iCasp9/AP1903-induced apoptotic MSCs (MSCiCasp9+) were tested in vitro and in in vivo models of acute colitis. Our data show a very similar and robust immunosuppressive and anti-inflammatory properties of both "parental" alive MSCGFP+ cells and apoptotic MSCiCasp9+ cells in vitro and in vivo regardless of whether apoptosis was induced in vivo or in vitro before administering MSCiCasp9+ lysates. This development of an efficient iCasp9 switch may potentiate the safety of MSC-based therapies in the case of an adverse event and, will also circumvent current logistic technical limitations and biological uncertainties associated to MSC-EVs.

Published
2022

17. A comprehensive single-cell expression atlas of human AML leukemia-initiating cells unravels the contribution of HIF pathway and its therapeutic potential

Author

Velasco-Hernandez, Talia, primary, Trincado, Juan L., additional, Vinyoles, Meritxell, additional, Closa, Adria, additional, Gutiérrez-Agüera, Francisco, additional, Molina, Oscar, additional, Rodríguez-Cortez, Virginia C, additional, Petazzi, Paolo, additional, Beneyto-Calabuig, Sergi, additional, Velten, Lars, additional, Romecin, Paola, additional, Casquero, Raquel, additional, Abollo-Jiménez, Fernando, additional, Díaz de la Guardia, Rafael, additional, Lorden, Patricia, additional, Bataller, Alex, additional, Lapillonne, Helene, additional, Stam, Ronald W, additional, Vives, Susana, additional, Torrebadell, Montserrat, additional, Fuster, Jose Luis, additional, Bueno, Clara, additional, Eyras, Eduardo, additional, Heyn, Holger, additional, and Menéndez, Pablo, additional

Published
2022
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18. The insecticides permethrin and chlorpyrifos show limited genotoxicity and no leukemogenic potential in human and murine hematopoietic stem progenitor cells

Author

Rodriguez-Cortez, Virginia C, primary, Navarrete-Meneses, Maria Pilar, additional, Molina, Oscar, additional, Velasco-Hernandez, Talia, additional, Gonzalez, Jessica, additional, Romecin, Paola, additional, Gutierrez-Aguera, Francisco, additional, Roca-Ho, Heleia, additional, Vinyoles, Meritxell, additional, Kowarz, Eric, additional, Marin, Pedro, additional, Rodriguez-Perales, Sandra, additional, Gomez-Marin, Carlos, additional, Perez-Vera, Patricia, additional, Cortes-Ledesma, Felipe, additional, Bigas, Anna, additional, Terron, Andrea, additional, Bueno, Clara, additional, and Menendez, Pablo, additional

Published
2021
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19. Enforced sialyl-Lewis-X (sLeX) display in E-selectin ligands by exofucosylation is dispensable for CD19-CAR T-cell activity and bone marrow homing

Author

Sánchez-Martínez, Diego, Gutiérrez-Agüera, Francisco, Romecin, Paola, Vinyoles, Meritxell, Palomo, Marta, Tirado, Néstor, Zanetti, SR, Juan, Manel, Carlet, Michela, Jeremias, Irmela, Menéndez, Pablo, Sánchez-Martínez, Diego, Gutiérrez-Agüera, Francisco, Romecin, Paola, Vinyoles, Meritxell, Palomo, Marta, Tirado, Néstor, Zanetti, SR, Juan, Manel, Carlet, Michela, Jeremias, Irmela, and Menéndez, Pablo

Abstract

Altres ajuts: Fundació La Caixa, CD19-directed chimeric antigen receptors (CAR) T cells induce impressive rates of complete response in advanced B-cell malignancies, specially in B-cell acute lymphoblastic leukemia (B-ALL). However, CAR T-cell-treated patients eventually progress due to poor CAR T-cell persistence and/or disease relapse. The bone marrow (BM) is the primary location for acute leukemia. The rapid/efficient colonization of the BM by systemically infused CD19-CAR T cells might enhance CAR T-cell activity and persistence, thus, offering clinical benefits. Circulating cells traffic to BM upon binding of tetrasaccharide sialyl-Lewis X (sLeX)-decorated E-selectin ligands (sialofucosylated) to the E-selectin receptor expressed in the vascular endothelium. sLeX-installation in E-selectin ligands is achieved through an ex vivo fucosylation reaction. Here, we sought to characterize the basal and cell-autonomous display of sLeX in CAR T-cells activated using different cytokines, and to assess whether exofucosylation of E-selectin ligands improves CD19-CAR T-cell activity and BM homing. We report that cell-autonomous sialofucosylation (sLeX display) steadily increases in culture- and in vivo -expanded CAR T cells, and that, the cytokines used during T-cell activation influence both the degree of such endogenous sialofucosylation and the CD19-CAR T-cell efficacy and persistence in vivo. However, glycoengineered enforced sialofucosylation of E-selectin ligands was dispensable for CD19-CAR T-cell activity and BM homing in multiple xenograft models regardless the cytokines employed for T-cell expansion, thus, representing a dispensable strategy for CD19-CAR T-cell therapy. Glyco-engineered enforced sialofucosylation of E-selectin ligands was dispensable for CD19-CAR T-cell activity and BM homing in multiple xenograft models, thus representing an unnecessary strategy for CD19-CAR T-cell therapy

Published
2021

20. Enforced sialyl‐Lewis‐X (sLeX) display in E‐selectin ligands by exofucosylation is dispensable for CD19‐CAR T‐cell activity and bone marrow homing

Author

Sánchez‐Martínez, Diego, primary, Gutiérrez‐Agüera, Francisco, additional, Romecin, Paola, additional, Vinyoles, Meritxell, additional, Palomo, Marta, additional, Tirado, Néstor, additional, Zanetti, Samanta Romina, additional, Juan, Manel, additional, Carlet, Michela, additional, Jeremias, Irmela, additional, and Menéndez, Pablo, additional

Published
2021
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22. 3138 – HIGH CHROMOSOME INSTABILITY ACROSS ANEUPLOID SUBTYPES OF CHILDHOOD B-CELL ALL AND POTENTIAL ASSOCIATION WITH DISEASE PROGRESSION IN PDX MODELS.

Author

Molina, Oscar, Thampi, namitha, Trincado, Juan, Ortega-Sabater, Carmen, Fernández-Calvo, Gabriel, Romecín, Paola, Martínez-Moreno, Alba, Velasco-Hernández, Talía, Vinyoles, Meritxell, Pérez-García, Victor, Merkel, Angelika, Granada, Isabel, Camós, Mireia, Fuster, Jose, Ballerini, Paola, Locatelli, Franco, Mullighan, Charles, Bueno, Clara, and Menéndez, Pablo

Published
2022
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24. Transcriptional Induction of Periostin by a Sulfatase 2–TGFβ1–SMAD Signaling Axis Mediates Tumor Angiogenesis in Hepatocellular Carcinoma

Author

Chen, Gang, primary, Nakamura, Ikuo, additional, Dhanasekaran, Renumathy, additional, Iguchi, Eriko, additional, Tolosa, Ezequiel J., additional, Romecin, Paola A., additional, Vera, Renzo E., additional, Almada, Luciana L., additional, Miamen, Alexander G., additional, Chaiteerakij, Roongruedee, additional, Zhou, Mengtao, additional, Asiedu, Michael K., additional, Moser, Catherine D., additional, Han, Shaoshan, additional, Hu, Chunling, additional, Banini, Bubu A., additional, Oseini, Abdul M., additional, Chen, Yichun, additional, Fang, Yong, additional, Yang, Dongye, additional, Shaleh, Hassan M., additional, Wang, Shaoqing, additional, Wu, Dehai, additional, Song, Tao, additional, Lee, Ju-Seog, additional, Thorgeirsson, Snorri S., additional, Chevet, Eric, additional, Shah, Vijay H., additional, Fernandez-Zapico, Martin E., additional, and Roberts, Lewis R., additional

Published
2017
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25. Platelet Reactivity in Hemophilia a. Flow Cytometry Study to Detect the Hemorragic Phenotype

Author

Melero-Amor, Antonia, primary, García Candel, Faustino, additional, García, David, additional, Romecin, Paola, additional, Iyu, David, additional, Cabañas-Perianes, Valentin, additional, Pérez, Raúl, additional, O´Connor, Jose-Enrique, additional, Moraleda, Jose M, additional, Marín, Noemí, additional, and Blanquer Blanquer, Miguel, additional

Published
2016
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26. Activation of the Transcription Factor GLI1 by WNT Signaling Underlies the Role of SULFATASE 2 as a Regulator of Tissue Regeneration

Author

Nakamura, Ikuo, primary, Fernandez-Barrena, Maite G., additional, Ortiz-Ruiz, Maria C., additional, Almada, Luciana L., additional, Hu, Chunling, additional, Elsawa, Sherine F., additional, Mills, Lisa D., additional, Romecin, Paola A., additional, Gulaid, Kadra H., additional, Moser, Catherine D., additional, Han, Jing-Jing, additional, Vrabel, Anne, additional, Hanse, Eric A., additional, Akogyeram, Nicholas A., additional, Albrecht, Jeffrey H., additional, Monga, Satdarshan P.S., additional, Sanderson, Schuyler O., additional, Prieto, Jesus, additional, Roberts, Lewis R., additional, and Fernandez-Zapico, Martin E., additional

Published
2013
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27. The insecticides permethrin and chlorpyrifos show limited genotoxicity and no leukemogenic potential in human and murine hematopoietic stem progenitor cells.

Author

Rodriguez-Cortez VC, Navarrete-Meneses MP, Molina O, Velasco-Hernandez T, Gonzalez J, Romecin P, Gutierrez-Aguera F, Roca-Ho H, Vinyoles M, Kowarz E, Marin P, Rodriguez-Perales S, Gomez-Marin C, Perez-Vera P, Cortes-Ledesma F, Bigas A, Terron A, Bueno C, and Menendez P

Subjects
Animals, Hematopoietic Stem Cells, Humans, Mice, Permethrin toxicity, Chlorpyrifos toxicity, Insecticides toxicity
Published
2022
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