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Natural toxins, mainly small molecules, are a category of chemical hazards in agri-food systems that pose threats to both public health and food security. Current standard methods for monitoring these toxins, predominantly based on liquid chromatography-mass spectrometry, are costly, labor-intensive, and complex. This study presents the development of a novel microfluidic optical aptasensor for rapid detection of small molecules based on analyte-tuned growth of gold nanoseeds combined with machine learning-enhanced spectrum analysis. We discovered and optimized a previously unreported growth pattern of aptamer-coated nanoparticles in the presence of different concentrations of analyte, enabling the detection of a major mycotoxin in food. The entire analysis was miniaturized on a customized microfluidic platform, allowing for automated spectral acquisition with precise liquid manipulation. A machine learning model, based on random forest with feature engineering, was developed and evaluated for spectrum analysis, significantly enhancing the prediction of mycotoxin concentrations. This approach extended the detection limit determined by the conventional method (∼72 ppb with high variation) to a wider range of 10 ppb to 100 ppm with high accuracy (overall mean absolute percentage error of 5.7%). The developed analytical tool provides a promising solution for detecting small molecules and monitoring chemical hazards in agri-food systems and the environment.

The food industry is increasingly focused on maintaining the quality and safety of food products as consumers are becoming more health conscious and seeking fresh, minimally processed foods. However, deterioration and spoilage caused by foodborne pathogens continue to pose significant challenges, leading to decreased shelf life and quality. To overcome this issue, the food industry and researchers are exploring new approaches to prevent microbial growth in food, while preserving its nutritional value and safety. Active packaging, including antimicrobial packaging, has gained considerable attention among current food packaging methods owing to the wide range of materials used, application methods, and their ability to protect various food products. Both direct and indirect methods can be used to improve food safety and quality by incorporating antimicrobial compounds into the food packaging materials. This comprehensive review focuses on natural and synthetic antimicrobial substances and polymer-based films, and their mechanisms and applications in packaging systems. The properties of these materials are compared, and the persistent challenges in the field of active packaging are emphasized. Specifically, there is a need to achieve the controlled release of antimicrobial agents and develop active packaging materials that possess the necessary mechanical and barrier properties, as well as other characteristics essential for ensuring food protection and safety, particularly bio-based packaging materials.

Mycotoxins are secondary metabolites of certain moulds, prevalent in 60-80% of food crops and many processed products but challenging to eliminate. Consuming mycotoxin-contaminated food and feed can lead to various adverse effects on humans and livestock. Therefore, testing mycotoxin residue levels is critical to ensure food safety. Gold standard analytical methods rely on liquid chromatography coupled with optical detectors or mass spectrometers, which are high-cost with limited capacity. This study reported the successful development of a microfluidic "lab-on-a-chip" device to enrich and detect zearalenone in food samples based on the fluorescence quenching effect of quantum dots and selective affinity of molecularly imprinted polymers (MIPs). The dummy template and functional polymer were synthesized and characterized, and the detailed microfluidic chip design and optimization of the flow conditions in the enrichment module were discussed. The device achieved an enrichment factor of 9.6 (±0.5) in 10 min to quantify zearalenone spiked in food with high recoveries (91-105%) at 1-10 mg kg -1 , covering the concerned residue levels in the regulations. Each sample-to-answer test took only 20 min, involving 3 min of manual operation and no advanced equipment. This microfluidic device was mostly reusable, with a replaceable detection module compatible with fluorescence measurement using a handheld fluorometer. To our best knowledge, the reported device was the first application of an MIP-based microfluidic sensor for detecting mycotoxin in real food samples, providing a novel, rapid, portable, and cost-effective tool for monitoring mycotoxin contamination for food safety and security.

Chickpea protein (CPI) is a promising dietary protein and potential substitute for soy protein in food product development due to its high protein content and low allergenicity. However, CPI possesses denser tertiary and quaternary structures and contains certain amount of anti-nutritional factors, both of which constrain its functional properties and digestibility. The objective of this study was to assess the effectiveness of atmospheric pressure plasma jets (APPJ) as a non-thermal method for enhancing the functional characteristics and digestibility of CPI. In this study, the reactive oxygen and nitrogen species generated by the APPJ treatment led to protein oxidation and increased carbonyl and di-tyrosine contents. At the same time, the secondary, tertiary and microstructural structures of CPI were changed. The solubility, water holding capacity, fat absorption capacity, emulsifying capacity and foaming capacity of CPI were significantly improved after 30 s APPJ treatment, and a higher storage modulus in rheology was observed. Additionally, it was observed that the in vitro protein digestibility (IVPD) of APPJ-treated CPI increased significantly from 44.85 ± 0.6 % to 50.2 ± 0.59 % following in vitro simulated gastric and intestinal digestion, marking a noteworthy improvement of 11.93 %. These findings indicate that APPJ processing can enhance the functional and digestive properties of CPI through structural modification and expand its potential applications within the food industry., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Biosorbents for water remediation were prepared using keratin biopolymer cross-linked with nanochitosan (NC). Keratin proteins were dissolved using reducing agents and NC was incorporated with concentrations of 1, 3 and 5 % individually into the keratin solution. The mixtures were thermally treated at 75°C overnight, which promoted the formation of ester bonds between the hydroxyl groups of nanochitosan and the carboxylic groups of the keratin biopolymer. The resulting keratin derived biosorbents were characterized by X-Ray photoelectron spectroscopy, confirming the cross-linking between keratin and nanochitosan. The chicken feathers keratin (CFK) surface modifications with nanochitosan were examined with Brunauer-Emmett-Teller, scanning and transmission electron microscopies. The sorption capacity of biosorbents was tested for eight different metals simultaneously at different contact times (15, 30, 60, 120, 240, 280 mins) and pH (5.5, 7.5 and 8.5), including arsenic, selenium, chromium, nickel, cobalt, lead, cadmium and zinc, using simulated industrial wastewater water containing 600 μgl -1 concentration of each metal. The synthesized environmentally benign biosorbents exhibited biosorption of metals upto 98 % at pH 7.5 and a contact time of 24 h, showing their potential for industrial wastewater remediation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

We report the development of a competitive ELISA-based origami microfluidic paper-based analytical device (μPAD) for the detection of mycotoxins in animal feed material. The μPAD was patterned using the wax printing technique with the design of a testing pad in the middle and two absorption pads at the side. Anti-mycotoxin antibodies were effectively immobilized on chitosan-glutaraldehyde-modified sample reservoirs in the μPAD. The determination of zearalenone, deoxynivalenol, and T-2 toxin in corn flour was successfully achieved by performing competitive ELISA on the μPAD in 20 min. Colorimetric results were easily distinguished by the naked eye with a detection limit of 1 µg/mL for all three mycotoxins. The μPAD integrated with competitive ELISA holds potential for practical applications in the livestock industry for rapid, sensitive, and cost-effective detection of different mycotoxins in animal feed materials., (© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)

Atmospheric cold plasma (ACP) treatment can reduce bacterial pathogens in foods. Additional reduction in bacterial cells during storage after ACP treatment was previously reported. The underlying mechanisms of bacterial inactivation during ACP treatment and post-treatment storage need to be understood. This study investigated the changes in the morpho-physiological status of Listeria monocytogenes on ham surfaces after post-ACP-treatment storage of 1 h, 24 h, and 7 days at 4 °C. The membrane integrity, intracellular oxidative stress, and esterase activity of L. monocytogenes were evaluated by flow cytometry. L. monocytogenes cells were under high oxidative stress conditions with slightly permeabilized membranes after 1 h of post-ACP-treatment storage according to the flow cytometry data. During the extended storage of 24 h, the percentage of cells with a slightly permeabilized membrane increased; subsequently, the percentage of cells with intact membranes decreased. The percentage of L. monocytogenes cells with intact membranes decreased to <5% with a treatment time of 10 min and after 7 days of post-treatment storage. In addition, the percentage of L. monocytogenes cells under oxidation stress decreased to <1%, whereas the percentage of cells with completely permeabilized membranes increased to more than 90% for samples treated with ACP for 10 min and 7 days of post-treatment storage. With increased ACP treatment time, for 1 h stored samples, the percentage of cells with active esterase and slightly permeabilized membranes increased. However, during the extended post-treatment storage of 7 days, the percentage of cells with active esterase and slightly permeabilized membranes decreased to below 1%. At the same time, the percentage of cells with permeabilized membrane increased to more than 92% with an increase in ACP treatment time of 10 min. In conclusion, the higher inactivation after 24 h and 7 days post-ACP-treatment storage compared to 1 h stored samples correlated with the loss of esterase activity and membrane integrity of L. monocytogenes cells.

This study assessed the gel properties and conformational changes of duck myofibrillar protein (DMP) affected by plasma-activated water (PAW) generated at various discharge times (0 s, 10 s, 20 s, 30 s, and 40 s). With the treatment of PAW-20 s, the gel strength and water-holding capacity (WHC) of DMP gels were significantly increased when compared to the control group. Throughout the heating process, dynamic rheology revealed that the PAW-treated DMP had a higher storage modulus than the control. The hydrophobic interactions between protein molecules were significantly improved by PAW, resulting in a more ordered and homogeneous gel microstructure. The increased sulfhydryl and carbonyl content in DMP indicated a higher degree of protein oxidation with PAW treatment. Additionally, the circular dichroism spectroscopy demonstrated that PAW induced α-helix and β-turn transformed to β-sheet in DMP. Surface hydrophobicity, fluorescence spectroscopy, and UV absorption spectroscopy suggested that PAW altered DMP's tertiary structure, although the electrophoretic pattern indicated that the primary structure of DMP was mostly unaffected. These results suggest that PAW can improve the gel properties of DMP through mild alteration in its conformation.

Contamination of barley by deoxynivalenol (DON), a mycotoxin produced by Fusarium graminearum , causes considerable financial loss to the grain and malting industries. In this study, two atmospheric cold plasma (ACP) reactors were used to produce plasma-activated water (PAW) bubbles. The potential of PAW bubbles for the steeping of naturally infected barley (NIB) during the malting process was investigated. The PAW bubbles produced by treating water for 30 min using a bubble spark discharge (BSD) at low temperature resulted in the greatest concentration of oxygen-nitrogen reactive species (RONS). This treatment resulted in 57.3% DON degradation compared with 36.9% in the control sample; however, the same treatment reduced germination significantly ( p < 0.05). Direct BSD ACP treatment for 20 min at low temperature and indirect treatment for 30 min increased the percentage of germinated rootlets of the seedlings compared with the control. Considering both the DON reduction and germination improvement of barley seeds, continuous jet ACP treatment for 30 min performed better than the other treatments used in this study. At higher temperature of PAW bubbles, the concentration of RONS was significantly ( p < 0.05) reduced. Based on quantitative polymerase chain reaction (qPCR) analysis and fungal culture tests, the PAW bubble treatment did not significantly reduce infection of NIB. Nonetheless, this study provides useful information for the malting industry for PAW treatment optimization and its use in barley steeping for DON reduction and germination improvement.

The COVID-19 is a crisis of unprecedented magnitude, which has resulted in countless casualties and security troubles. In view of recent events of corona virus people are required to wear face masks to protect themselves from getting infected. As a result, a good portion of face (nose and mouth) is hidden by the mask and hence the facial recognition becomes difficult. Many organizations use facial recognition as a means of authentication. Researchers focus on developing rapid and efficient solutions to deal with the ongoing coronavirus pandemic by coming up with suggestions for handling the facial recognition problem. This research paper aims to identify the person, while the face is covered with a facial mask with only eyes and forehead being exposed. The first step involves marking the facial region. Next, using the data set, we will implement an object detection model YOLOv3 to identify unmasked and masked faces. The YOLO v3 object detection model is the best performing model with a detection time of 0.012 s, F 1 score of 0.90 and mAP score of 0.92. Experimental results on Real-World Masked-Face-Data set show high recognition performance., Competing Interests: Conflicts of interestThe authors declare that they have no conflicts of interest to report regarding the present study., (© The Author(s), under exclusive licence to Springer Nature Singapore Pte Ltd 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

In this study, we employed a facile method to synthesize feather keratin derived biosorbents using water dispersed graphene oxide. The successful cross-linking of feather keratin with graphene oxide was investigated through X-ray photoelectrons spectroscopy (XPS), scanning and transmission electron microscopy, and Brunauer-Emmett-Teller (BET) analysis. The modifications resulted in increased surface area of the keratin proteins with substantial morphological changes including the development of cracked and rough patches on the surface. The chicken feather keratin/graphene oxide based biosorbents exhibited excellent performance for the simultaneous removal of metal oxyanions including arsenic (As), selenium (Se), chromium (Cr) and cations including nickel (Ni), cobalt (Co), lead (Pb), cadmium (Cd) and zinc (Zn) up to 99%, from polluted synthetic water containing 600 μgL -1 of each metal concentration in 24 h. The insights into the biosorption mechanism revealed that the electrostatic interaction, chelation and complexation primarily contributed to the removal of multiple heavy metal ions in a single treatment. This study has demonstrated that modification of chicken feather keratin with graphene oxide is an effective way to improve its sorption capacity for removing multiple trace metal ions from contaminated water., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Background: Ready-to-eat fruit and vegetable products have gained tremendous popularity in recent years. The main challenges associated with these minimally processed products are their short shelf life and high food safety concerns. In this study, our goal was to develop an integrated process to both reduce the Salmonella population by >5 log CFU g -1 ) and to reduce polyphenol oxidase activity, followed by quickly cooling the product. We compared the effect of a sequential treatment of dipping in citric acid (CA) followed by cold plasma (CP) treatment on the inactivation of Salmonella Typhimurium, polyphenol oxidase (PPO) activity, browning, total phenolic content and the moisture loss of cut apples during the plasma-integrated low-pressure cooling (PiLPC) process., Results: The greatest inactivation of Salmonella (5.68 log CFU g -1 ) and the highest PPO inactivation (78%) were observed after dipping cut apples in 5% CA, followed by 3 min of CP treatment. The color of cut apples remained relatively unchanged, with a fresh-like appearance during 7 days of storage at 4 °C after this combined treatment. Although the low-pressure cooling time was increased when samples were pre-dipped in CA, related to those undipped, the moisture loss was reduced by more than 50% during the PiLPC process. No significant reduction in phenolic content was observed during the PiLPC when the samples were pre-dipped in 5% CA., Conclusion: These results indicate the potential of this integrated process for the inactivation of endogenous food enzymes and bacterial pathogens in fresh-cut apples. © 2021 Society of Chemical Industry., (© 2021 Society of Chemical Industry.)

Salmonella, a foodborne pathogen, has been frequently associated with recalls of fresh food products, including poultry meat products. Atmospheric cold plasma (ACP) is a novel non-thermal technology, which has potential to reduce pathogens in food products. This study demonstrates the synergistic interactions of food grade organic acids (i.e., lactic acid (LA) or gallic acid (GA)) and ACP to inactivate Salmonella enterica Typhimurium ATCC 13311 inoculated on polycarbonate membrane filter paper and poultry meat surface. Organic acids were used in the form of a spray to enhance dispersion on samples surface. The sequential treatment of organic acid followed by ACP synergistically reduced S. Typhimurium on poultry meat surface. Irrespective of the type of organic acid, an average reduction of more than 3.5 log CFU/cm 2 in S. Typhimurium on filter paper was obtained, when a combination of 10 mM LA or GA with an ACP exposure of 30 s was tested. However, the individual treatments of LA, GA, and ACP resulted in only 0.4, 0.3, 1.2 log CFU/cm 2 reduction in S. Typhimurium, respectively. On poultry meat surface, a higher level of organic acid concentration (i.e., 50 mM) in combination with 30 s ACP was required to achieve more than 2.5 log CFU/cm 2 reduction in S. Typhimurium. Our investigation on inactivation mechanisms revealed that the sequential treatment of LA or GA with ACP resulted in a significantly higher level of membrane permeability and membrane lipid peroxidation in S. Typhimurium cells. Additionally, the combined treatment significantly reduced the cell metabolic activity and affected the intracellular reactive oxygen species level of S. Typhimurium. In summary, this study demonstrated the potential synergistic benefits of combining organic acids and ACP to achieve a higher level of bacterial inactivation., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Foodborne outbreaks and recalls of pathogen-contaminated low-moisture foods (LMFs, foods with water activity at 25 °C < 0.85) have led to numerous scientific studies on bacterial persistence, as well as newly developed industrial interventions. Conducting microbial tests of LMFs, lab tests, or validation studies in pilot plans requires complete information on protocols and parameters that need to be aware of-in particular, understanding how factors influence the thermal resistance of bacterial pathogen in LMFs is critical in designing any thermal processes. This review provides detailed information on the general protocols of microbial studies of LMFs: from pertinent pathogen identification to microbial validation studies. In particular, it reviewed the detailed procedures (e.g., lawn-harvest method), analytical protocols (e.g., recovery and enumeration of pathogens in LMFs), and specialized tools that have been utilized (even widely accepted) in laboratory-based microbial studies of LMFs. It also summarized the factors that influence the microbial validation studies. This article could support the intervention of existing pasteurization processes in the LMF industry, promoting the microbial safety of LMFs., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Pea proteins are increasingly used as an alternative for soy protein, however, structuring of pea protein remains a challenge due to its low gelling capacity. This study reports a pea protein pre-treatment that combines atmospheric cold plasma (ACP) and pH-shifting to improve pea protein gelling property. Specifically, the pea protein concentrate was treated by ACP at pH 12 for 10 min, followed by pH-shifting to neutral and spray-drying to prepare PPC treated powders. Although, pea protein concentrate did not form a self-standing gel until 95 °C for 60 min, PPC treated suspension (14 wt%) formed gels with good mechanical properties (compressive strength: 2.81 kPa) by heating at 70 °C within 10-20 min. The strength of the gel made from PPC treated was further increased to 3.70 and 5.53 kPa when raising the temperature to 80 and 90 °C, respectively. The protein characterizations revealed that the combined ACP and pH-shifting treatment partially unfolded pea protein by altering the tertiary structure, and then the active species produced by ACP facilitated the formation of protein aggregates with increased surface hydrophobicity. During heating, the aggregates served as active building blocks to form more ordered three-dimensional gel networks via hydrophobic interactions and hydrogen bonding. The greatly increased gel strength and the powder form will allow pea protein to be widely used as a gelling ingredient in many food formulations. The capacity to form gels at a reduced temperature than pea protein denaturation temperature (∼95 °C) will enable food texture development by conventional cooking., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Water sorption isotherms of bacteria reflect the water activity with the change of moisture content of bacteria at a specific temperature. The temperature-dependency of water activity change can help to understand the thermal resistance of bacteria during a thermal process. Thermal resistance of bacteria in low-moisture foods may differ significantly depending on the physiological characteristics of microorganisms, including cell structure, existence of biofilms, and growth state. Previous studies demonstrated that the incremental change of a w in bacterial cells during thermal treatments resulted in changes in their thermotolerance. In this study, a pathogen associated with low-moisture foods outbreaks, Salmonella Enteritidis PT30 (in planktonic and biofilm forms), and its validated surrogate, Enterococcus faecium, were lyophilized and their water sorption isotherms (WSI) at 20, 40, and 60 °C were determined by using a vapor sorption analyzer and simulated by the Guggenheim, Anderson and De Boer model (GAB). The published thermal death times at 80 °C (D 80 °C -values) of these bacteria in low-moisture environments were related with their WSI-derived a w changes. The results showed that planktonic E. faecium and biofilms of Salmonella, exhibiting higher thermal resistance compared to the planktonic cultures of Salmonella, had a smaller increase in a w when thermally treated from 20 to 60 °C in sealed test cells. The computational modeling also showed that when temperature increased from 20 to 60 °C, with an increase in relative humidity from 10% to 60%, freeze-dried planktonic E. faecium and Salmonella cells would equilibrate to their surrounding environments in 0.15 s and 0.25 s, respectively, suggesting a rapid equilibration of bacterial cells to their microenvironment. However, control of bacteria with different cell structure and growth state would require further attentions on process design adjustment because of their different water sorption characteristics., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Deoxynivalenol (DON) is one of the main trichothecenes, that causes health-related issues in humans and animals and imposes considerable financial loss to the food industry each year. Numerous treatments have been reported in the literature on the degradation of DON in food products. These treatments include thermal, chemical, biological/enzymatic, irradiation, light, ultrasound, ozone, and atmospheric cold plasma treatments. Each of these methods has different degradation efficacy and degrades DON by a distinct mechanism, which leads to various degradation byproducts with different toxicity. This manuscript focuses to review the degradation of DON by the aforementioned treatments, the chemical structure and toxicity of the byproducts, and the degradation pathway of DON. Based on the type of treatment, DON can be degraded to norDONs A-F, DON lactones, and ozonolysis products or transformed into de-epoxy deoxynivalenol, DON-3-glucoside, 3-acetyl-DON, 7-acetyl-DON, 15-acetyl-DON, 3-keto-DON, or 3-epi-DON. DON is a major problem for the grain industry and the studies focusing on DON degradation mechanisms could be helpful to select the best method and overcome the DON contamination in grains.

Decontamination of low water activity (a w ) foods, like pet foods is a challenging task. Treatment using light emitting diode (LED) is an emerging decontamination method, that can induce photodynamic inactivation in bacteria. The objective of this study was to understand the effect of selected product and process parameters on the antibacterial efficacy of treatment using light pulses of 455 nm wavelength on dry powdered Salmonella and pet foods equilibrated to 0.75 a w . The surface temperature increase, weight loss, and a w decrease in the samples were determined after LED treatments with different doses. S. Typhimurium on pet foods showed better sensitivity to 455 nm LED treatment than the powdered S. Typhimurium. For instance, 455 nm LED treatment with 785.7 J/cm 2 dose produced a log reduction of 1.44 log (CFU/g) in powdered S. Typhimurium population compared to 3.22 log (CFU/g) on pet food. The LED treatment was less effective against 5-strain cocktail of Salmonella in low a w pet foods. The treated samples showed significant reduction in weight and a w showing the heating and drying potential of 455 nm LED treatment. Significant lipid oxidation was observed in the treated pet foods. Overall, the dose, treatment time, and sample type influenced the Salmonella inactivation efficacy of 455 nm LED treatment in low a w conditions., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Salmonella contamination of low-water activity (a w ) foods poses a serious concern worldwide. The present study was conducted to assess the effects of drying conditions, food composition, and water activity on the desiccation tolerance and thermal resistance of S. Enteritidis FUA1946, S. Senftenberg ATCC43845 and S. Typhimurium ATCC13311 in pet food, binder formulation, and skim milk powder. The samples were wet inoculated with the individual Salmonella strains and were equilibrated to a w 0.33 and 0.75, followed by an isothermal treatment at 70 °C. The thermal inactivation data was fitted to the Weibull model. Irrespective of the a w , food composition and physical structure of the selected foods, strain S. Enteritidis FUA1946 displayed the highest desiccation and thermal resistance, followed by S. Senftenberg ATCC43845 and S. Typhimurium ATCC13311. The food matrix and strain type significantly (p < 0.05) influenced the thermal resistance of microorganisms in foods along with a w change during thermal treatments. To further study the effect of food composition, an additional set of experiments using dry inoculation of the resistant Salmonella strain in the low-a w foods was designed. Significant (p < 0.05) matrix-dependent interaction on Salmonella reduction was observed. The water adsorption isotherms of selected low-a w foods were measured at 20 and 70 °C to relate the thermal inactivation kinetics with the change in the a w . The characterization of thermal resistance of the Salmonella serovars in low-a w products with different compositions and a w in this study may be used for the validation of thermal challenge studies., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Fresh food products, including fruits, vegetables, raw meat, and poultry, have been associated with safety concerns and quality issues, owing to their susceptibility to rapid deterioration and microbial contamination. This research aimed to develop an integrated process to simultaneously cool and decontaminate high moisture food products. Cold plasma (CP), a novel decontamination technology, was integrated with vacuum cooling to develop a plasma integrated low-pressure cooling (PiLPC) process. To evaluate the rapid cooling and microbial inactivation efficacies of the PiLPC process, fresh cut Granny Smith apples andSalmonella entericaserovarTyphimurium ATCC 13311 were used as the model food and microorganism, respectively. The influence of process parameters including treatment time, pressure, and post-treatment storage, on the inactivation ofSalmonellaon fresh-cut apples was investigated.Inactivation ofSalmonellaincreased with treatment time, with a maximum reduction of 3.21 log CFU/g after 5 min of CP treatment at atmospheric pressure. Inactivationof Salmonellaafter CP treatment at 200 mbar was not significantly different from that at atmospheric pressure for the same treatment time. CP treatment of 3 min at 200 mbar followed by a post-treatment storage of 3 days at 4 °C reduced the totalSalmonellapopulation on cut apple slices by > 6 log CFU/g. The temperature of the cut apples was reduced from room temperature to 2 °Cin 3 to 9 min depending on the sample surface area to volume ratio, when the pressure was reducedto 7 mbar. However, this PiLPC process resulted in moisture loss in cut apples. The results of this study indicate the potential of the PiLPC process for rapid cooling and microbial inactivation of fresh food products in a single process., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Phytic acid (PA) is the primary phosphorus reserve in cereals and legumes which serves the biosynthesis needs of growing tissues during germination. It is generally considered to be an anti-nutritional factor found in grains because it can bind to minerals, proteins, and starch, limiting their bioavailability. However, this same mineral binding property can also confer a number of health benefits such as reducing the risk of certain cancers, supporting heart health, and managing renal stones. In addition, the ability of PA to bind minerals allows it to be used in certain food quality applications such as stabilizing the green color of vegetables, preventing lipid peroxidation, and reducing enzymatic browning in fruits/vegetables. These beneficial properties create a potential for added-value applications in the utilization of PA in many new areas. Many possible processing techniques for the preparation of raw materials in the food industry can be used to reduce the concentration of PA in foods to mitigate its anti-nutritional effects. In turn, the recovered PA by-products could be available for novel uses. In this review, a general overview of the beneficial and anti-nutritional effects of PA will be discussed and then dephytinization methods will be explained., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Wheat (Triticum aestivum) is susceptible to mycotoxin contamination, which can result in significant health risks and economic losses. This research examined the ability of air atmospheric cold plasma (air-ACP) treatment to reduce pure and spiked T-2 and HT-2 mycotoxins' concentration on wheat grains. This study also evaluated the effect of ACP treatment using different gases on wheat grain germination parameters. The T-2 and HT-2 mycotoxin solutions applied on round cover-glass were placed on microscopy slides and wheat grains (0.5 g) were individually spiked with T-2 and HT-2 on their surfaces. Samples were then dried at room temperature (∼24 °C) and treated by air-ACP for 1 to 10 min. Ten minutes of air-ACP treatment significantly reduced pure T-2 and HT-2 concentrations by 63.63% and 51.5%, respectively. For mycotoxin spiked on wheat grains, 10 min air-ACP treatment significantly decreased T-2 and HT-2 concentrations up to 79.8% and 70.4%, respectively. No significant change in the measured quality and color parameters was observed in the ACP-treated samples. Wheat grain germination parameters were not significantly different, when treated with ACP using different gases. Air-ACP treatment and ACP treatment using 80% nitrogen + 20% oxygen improved the germination of wheat grains by 10% and 6%, respectively. This study demonstrated that ACP is an innovative technology with the potential to improve the safety of wheat grains by reducing T-2/HT-2 mycotoxins with an additional advantage of improving their germination. PRACTICAL APPLICATION: Atmospheric cold plasma (ACP) technology has a huge potential to degrade mycotoxins in food grains. This study evaluated the efficacy of ACP to reduce two major mycotoxins (T-2 and HT-2 toxins) in wheat grains. The results of this study will help to develop and scale-up the ACP technology for mycotoxin degradation in grains., (© 2021 Institute of Food Technologists®.)

Atmospheric cold plasma (ACP) is an emerging technology in the food industry with a huge antimicrobial potential to improve safety and extend the shelf life of food products. Dielectric barrier discharge (DBD) is a popular approach for generating ACP. Thanks to the numerous advantages of DBD ACP, it is proving to be successful in a number of applications, including microbial decontamination of foods. The antimicrobial efficacy of DBD ACP is influenced by multiple factors. This review presents an overview of ACP sources, with an emphasis on DBD, and an analysis of their antimicrobial efficacy in foods in open atmosphere and in-package modes. Specifically, the influence of process, product, and microbiological factors influencing the antimicrobial efficacy of DBD ACP are critically reviewed. DBD ACP is a promising technology that can improve food safety with minimal impact on food quality under optimal conditions. Once the issues pertinent to scale-up of plasma sources are appropriately addressed, the DBD ACP technology will find wider adaptation in food industry.

Low-moisture foods (LMF with water activity, a w  < 0.85) including pet foods and black pepper powder have consistently been associated with foodborne disease caused by Salmonella enterica. Increased heat resistance and prolonged survival at low-moisture conditions, however, remain major challenges to achieve effective inactivation of Salmonella in low-moisture foods. At low water activity (a w ) conditions, heat resistance of Salmonella is greatly enhanced when compared to high a w conditions. This study aimed to quantify the effect of a w on the heat resistance of Salmonella enterica in pet food pellets and black pepper powder. Pet food pellets were inoculated with two strains of heat resistant S. enterica and black pepper powder was inoculated with a 5-strain cocktail of Salmonella. Both inoculated food samples were equilibrated at 0.33, 0.54, and 0.75 a w in controlled humidity chambers. Inoculated pet food pellets and black pepper powder in closed aluminum cells were heat treated at specific temperatures for selected times. The results showed that the Weibull model fitted well the inactivation data. At a specific temperature, the rate of inactivation increased with the increase in the a w from 0.33 to 0.75, and the 3-log reduction times decreased for Salmonella in both food samples with the increase in a w . Water adsorption isotherms of pet food pellets and black pepper powder at initial and treatment temperatures were developed to understand the change in a w during heat treatments. The change in a w during heat treatment was dependent on the type of food matrix, which possibly influenced the thermal inactivation of Salmonella in pet food pellets and black pepper powder. The quantitative analysis of heat reduction of Salmonella with respect to a w aids in selection of the appropriate initial a w to develop effective heat treatment protocols for adequate reduction of Salmonella in pet foods and black pepper powder., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

One of the major advantages of 3D food printing is the customizability in terms of structure, design, and nutritional content. However, printability of the ingredients and the quality of the 3D printed food products are dependent on several product and printing parameters. In this study, nutrient dense cookies were developed with underutilized ingredients including jackfruit seed powder and finger millet powder as base materials using 3D food printing. The hardness, rheological behavior, and microstructure of 3D printed cookies with different products (e.g., water butter ratio) and printing (e.g., fill density and temperature) parameters were analyzed. The 3D printed cookies were developed by extruding at 27 and 30 °C with fill density values of 50%, 70%, 90%, and 100% and water butter ratios of 3:10 and 6:5. The 3D-printed cookie dough exhibited a more elastic behavior with higher storage modulus values than the loss modulus. The hardness of the baked cookies was influenced by printing temperature, fill density, and water butter ratio of 3D printed cookie dough and their interactions. The closed porosity of 3D printed cookies increased while the open porosity decreased with an increase in fill density. The baking times required were longer for 3D-printed cookies with higher fill density values. Overall, this study shows the importance of considering the specific ingredient and printing parameters to develop high quality 3D-printed cookies.

Listeria monocytogenes is often responsible for postprocessing contamination of ready-to-eat (RTE) products including cooked ham. As an emerging technology, atmospheric cold plasma (ACP) has the potential to inactivate L. monocytogenes in packaged RTE meats. The objectives of this study were to evaluate the effect of treatment time, modified atmosphere gas compositions (MAP), ham formulation, and post-treatment storage (1 and 7 days at 4 °C) on the reduction of a five-strain cocktail of L. monocytogenes and quality changes in ham subjected to in-package ACP treatment. Initial average cells population on ham surfaces were 8 log CFU/cm 2 . The ACP treatment time and gas composition significantly (P < 0.05) influenced the inactivation of L. monocytogenes, irrespective of ham formulations. When MAP1 (20% O 2 + 40% CO 2 + 40% N 2 ) was used, there was a significantly higher log reduction (>2 log reduction) in L. monocytogenes on ham in comparison to MAP2 (50% CO 2 + 50% N 2 ) and MAP3 (100% CO 2 ), irrespective of ham formulation. Addition of preservatives (that is, 0.1% sodium diacetate and 1.4% sodium lactate) or bacteriocins (that is, 0.05% of a partially purified culture ferment from Carnobacterium maltaromaticum UAL 307) did not significantly reduce cell counts of L. monocytogenes after ACP treatment. Regardless of type of ham, storage of 24 hr after ACP treatment significantly reduced cells counts of L. monocytogenes to approximately 4 log CFU/cm 2 . Following 7 days of storage after ACP treatment, L. monocytogenes counts were below the detection limit (>6 log reduction) when samples were stored in MAP1. However, there were significant changes in lipid oxidation and color after post-treatment storage. In conclusion, the antimicrobial efficacy of ACP is strongly influenced by gas composition inside the package and post-treatment storage. PRACTICAL APPLICATION: Surface contamination of RTE ham with L. monocytogenes may occur during processing steps such as slicing and packaging. In-package ACP is an emerging nonthermal technology, which can be used as a postpackaging decontamination step in industrial settings. This study demonstrated the influence of in-package gas composition, treatment time, post-treatment storage, and ham formulation on L. monocytogenes inactivation efficacy of ACP. Results of present study will be helpful to optimize in-package ACP treatment and storage conditions to reduce L. monocytogenes, while maintaining the quality of ham., (© 2020 Institute of Food Technologists®.)

Salmonella spp. is one of the top foodborne pathogens associated with low-moisture foods and they exhibit significant resistance to conventional thermal treatments. UV light pulses emitted from light emitting diode (LED) has shown antimicrobial potential in high-moisture foods and water. However, limited information is available about the antimicrobial potential of UV light with different wavelengths, including 395 nm in low-moisture foods. The objectives of this study were to investigate the antimicrobial potential of 395 nm pulsed LED light in wheat flour and the resulting quality changes. This study demonstrated a maximum 2.91 log reduction of Salmonella cocktail in wheat flour treated with 395 nm pulsed LED for 60 min in a semi-closed system. Oxidation occurred in wheat flour after 30 and 60 min exposure to the 395 nm LED, which subsequently led to bleaching, and polymerization of gluten components through disulphide linkage. The water holding capacity of gluten was reduced by oxidation, and the contents of secondary structures were altered significantly after pulsed LED treatment, but the rheological properties were not deteriorated. The disulfide bond formation naturally happens during dough formation and the oxidation triggered by pulsed LED treatment may play a role on accelerating this process. The 395 nm pulsed LED treatment could be a promising decontamination technology for wheat flour with an additional benefit of bleaching of the flour without chemicals. INDUSTRIAL RELEVANCE: A number of foodborne outbreaks and recalls have been related to low-moisture foods in these decades and recently several outbreaks were reported due to the occurrence of Salmonella in wheat flour. However, it is difficult to solve this problem through conventional thermal approaches because of the increased thermal resistance of Salmonella at low water activity environment. The emerging LED light source can produce light with monochromatic wavelengths without the use of mercury vapor lamps. It also has high durability, low heat generation, and is relatively easy to be adapted in an existing production line. Therefore, there is a great potential of using certain UV wavelengths emitted from LED to disinfect the low-moisture foods in food industries. To the best of our knowledge, no research was conducted on decontamination of wheat flour by using LEDs and only limited studies are available on the influence of pulsed LED treatment on food quality. The aim of this study was to explore the possibility of using 395 nm pulsed LED treatment as a novel tool for decontamination of Salmonella in a low-moisture food product (wheat flour) with industrial feasibility, and investigate the influence of the pulsed LED treatment on quality changes in the product., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

High intensity pulsed light emitting diode (LED) treatment is a novel approach to inactivate foodborne pathogens. The objective of this study was to evaluate the antibacterial potential of high intensity 365 (UV-A) and 395 nm (NUV-Vis) LED treatments against Escherichia coli and Salmonella enterica at high and low water activity (a w ) conditions, and to understand the influence of different process parameters on their antibacterial efficacy. Bacteria at high (in phosphate buffer saline, PBS) and low a w (a w = 0.75) conditions were treated with both the LEDs with specific doses at a fixed distance from the LEDs. The 365 nm LED showed more effectiveness in reducing the dried bacteria compared to 395 nm LED. The dry E. coli showed more resistance to LED treatments compared to Salmonella . The 365 and 395 nm LED treatments with ~658 J/cm 2 dose resulted in reductions of 0.79 and 1.76 log CFU/g of Salmonella , respectively, on 0.75 a w pet foods. The LED treatments increased the surface temperature, resulting in water loss in the treated samples. This study showed that the dose, duration of light exposure, bacterial strain, and a w played a major role in the antibacterial efficacy of the 365 and 395 nm LEDs.

Ready-to-eat (RTE) deli meat has been linked to several Listeria monocytogenes associated recalls. Recent studies demonstrated the potential antimicrobial effects of atmospheric cold plasma treatment on various food surfaces including RTE meat products. However, the influence of intrinsic and extrinsic factors, determining the efficacy of cold plasma to reduce Listeria has not been reported. This study investigated the influence of rosemary extract, salt (% NaCl), and treatment temperature on the efficacy of plasma to reduce numbers of L. innocua on RTE ham. The effect of post-treatment storage on L. innocua inactivation was also investigated. When the cold plasma treatment temperature was 4 °C, we observed a significant reduction in L. innocua of 1.75 and 1.51 log CFU/cm 2 on 1% and 3% NaCl ham surface without rosemary extract respectively, after 180 s treatment. At a treatment temperature of 23 °C, the L. innocua cells were reduced by 1.78 and 1.43 log CFU/cm 2 , respectively on these surfaces after 180 s. No significant effects of salt concentration and treatment temperature were observed on L. innocua inactivation during cold plasma treatment of ham. The post treatment storage at 4 °C for 6 h after 180 s of plasma treatment enhanced further reduction of L. innocua on 1% NaCl ham without rosemary. We also observed the increased concentration of malondialdehyde (MDA) equivalent lipid oxidation of plasma treated samples and was significantly higher (1.53 MDA mg/ kg ham) compared to untreated samples (0.92 MDA mg/kg ham). However, no significant differences in surface color parameters, L* and b* values were observed after plasma treatment, except a significant increase in a* values. The water content of plasma exposed samples decreased significantly for all treatment conditions whereas the water activity values were not changed significantly. In conclusion, the atmospheric cold plasma could be applied as a means for surface decontamination of RTE ham. However, the drying and oxidation of ham should be controlled in an open atmospheric plasma treatment condition., (Copyright © 2019. Published by Elsevier Ltd.)

Highlights: Atmospheric cold plasma and peracetic acid-based hurdle approach for safety of poultry products was evaluated. Study demonstrates a significant synergetic approach to reducing Salmonella on raw poultry. Hurdle approach shows promising bacterial reduction but requires further optimization.

Cold plasma treatment is a promising intervention in food processing to boost product safety and extend the shelf-life. The activated chemical species of cold plasma can act rapidly against micro-organisms at ambient temperatures without leaving any known chemical residues. This review presents an overview of the action of cold plasma against molds and mycotoxins, the underlying mechanisms, and applications for ensuring food safety and quality. The cold plasma species act on multiple sites of a fungal cell resulting in loss of function and structure, and ultimately cell death. Likewise, the species cause chemical breakdown of mycotoxins through various pathways resulting in degradation products that are known to be less toxic. We argue that the preliminary reports from cold plasma research point at good potential of plasma for shelf-life extension and quality retention of foods. Some of the notable food sectors which could benefit from antimycotic and antimycotoxin efficacy of cold plasma include, the fresh produce, food grains, nuts, spices, herbs, dried meat and fish industries., (© 2018 Institute of Food Technologists®.)