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370 results on '"Rosenblatt,Mark I"'

3. Preclinical Evaluation of the Safety and Efficacy of Cryopreserved Bone Marrow Mesenchymal Stromal Cells for Corneal Repair

Author

Putra, Ilham, Shen, Xiang, Anwar, Khandaker N, Rabiee, Behnam, Samaeekia, Ravand, Almazyad, Enmar, Giri, Pushpanjali, Jabbehdari, Sayena, Hayat, Mohammed R, Elhusseiny, Abdelrahman M, Ghassemi, Mahmood, Mahmud, Nadim, Edward, Deepak P, Joslin, Charlotte E, Rosenblatt, Mark I, Dana, Reza, Eslani, Medi, Hematti, Peiman, and Djalilian, Ali R

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Ophthalmology and Optometry, Regenerative Medicine, Eye Disease and Disorders of Vision, Stem Cell Research - Nonembryonic - Human, Stem Cell Research, Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, Eye, Animals, Bone Marrow, Clinical Trials, Phase I as Topic, Cornea, Corneal Injuries, Mesenchymal Stem Cells, Mice, Rabbits, Rats, Wound Healing, Biomedical Engineering, Opthalmology and Optometry, Ophthalmology and optometry
Abstract

PurposeMesenchymal stromal cells (MSCs) have been shown to enhance tissue repair as a cell-based therapy. In preparation for a phase I clinical study, we evaluated the safety, dosing, and efficacy of bone marrow-derived MSCs after subconjunctival injection in preclinical animal models of mice, rats, and rabbits.MethodsHuman bone marrow-derived MSCs were expanded to passage 4 and cryopreserved. Viability of MSCs after thawing and injection through small-gauge needles was evaluated by vital dye staining. The in vivo safety of human and rabbit MSCs was studied by subconjunctivally injecting MSCs in rabbits with follow-up to 90 days. The potency of MSCs on accelerating wound healing was evaluated in vitro using a scratch assay and in vivo using 2-mm corneal epithelial debridement wounds in mice. Human MSCs were tracked after subconjunctival injection in rat and rabbit eyes.ResultsThe viability of MSCs after thawing and immediate injection through 27- and 30-gauge needles was 93.1% ± 2.1% and 94.9% ± 1.3%, respectively. Rabbit eyes demonstrated mild self-limiting conjunctival inflammation at the site of injection with human but not rabbit MSCs. In scratch assay, the mean wound healing area was 93.5% ± 12.1% in epithelial cells co-cultured with MSCs compared with 40.8% ± 23.1% in controls. At 24 hours after wounding, all MSC-injected murine eyes had 100% corneal wound closure compared with 79.9% ± 5.5% in controls. Human MSCs were detectable in the subconjunctival area and peripheral cornea at 14 days after injection.ConclusionsSubconjunctival administration of MSCs is safe and effective in promoting corneal epithelial wound healing in animal models.Translational relevanceThese results provide preclinical data to support a phase I clinical study.

Published
2021

5. Keratoprosthesis

Author

Park, Jennifer, Karas, Faris I., de la Cruz, Jose, Cortina, M. Soledad, Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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8. Interstitial Keratitis

Author

Tu, Elmer Y., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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9. Corneal Dysgeneses, Dystrophies, and Degenerations

Author

Cockerham, Glenn C., Kenyon, Kenneth R., Hersh, Peter S., Azar, Dimitri, Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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10. Endothelial Keratoplasty

Author

Hill, Jordan R., Terry, Mark A., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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11. Ocular Surface Transplantation and Amniotic Membrane Surgery

Author

Niparugs, Muanploy, Nonpassopon, Manachai, Shareef, Farrah, Elhusseiny, Abdelrahman M., Djalilian, Ali R., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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12. Tumors of the Cornea and Conjunctiva

Author

Farooq, Asim V., Colby, Kathryn A., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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13. Treatment of Anterior Stromal Disorders

Author

Volante, Vincent, Cabot, Florence, Yoo, Sonia H., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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16. Corneal Manifestations of Metabolic Disease

Author

Syed, Zeba A., Meghpara, Beeran B., Hammersmith, Kristin M., Rapuano, Christopher J., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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17. Dry Eye Diagnosis and Management

Author

Sunshine, Sarah B., Dhall, Nikhil, Mona, Harissi-Dagher, Dana, M. Reza, Mun, Christine, Jain, Sandeep, Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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18. Fungal and Acanthamoeba Keratitis

Author

Alizadeh, Hassan, O’Day, Denis, Niederkorn, Jerry Y., Afshari, Natalie A., Garff, Kevin, McCulley, James P., Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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21. Corneal Physiology: Corneal Form and Function

Author

Klyce, Stephen D., Hallak, Joelle, Romond, Kathleen, Azar, Dimitri T., Kim, Tae-im, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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22. Clinical Examination and Diagnostic Testing

Author

Kang, Kai, Rosenblatt, Mark, Azar, Dimitri T., Section editor, Rosenblatt, Mark I., Section editor, Albert, Daniel M., editor, Miller, Joan W., editor, Azar, Dimitri T., editor, and Young, Lucy H., editor

Published
2022
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24. Multicenter prospective validation study for international chronic ocular graft-versus-host disease consensus diagnostic criteria

Author

Ogawa, Yoko, Dana, Reza, Kim, Stella, Jain, Sandeep, Rosenblatt, Mark I., Perez, Victor L., Clayton, Janine A., Alves, Monica, Rocha, Eduardo Melani, Amparo, Francisco, Seo, Kyoung Yul, Wang, Yan, Shen, Joanne, Oh, Joo Youn, Vanathi, Murugesan, Nair, Sridevi, Na, Kyung-Sun, Riemens, Anjo, Sippel, Kimberly, Soifer, Matias, Wang, Shudan, Trindade, Marilia, Kim, Mee Kum, Yoon, Chang Ho, Yagi, Ryuichiro, Hiratsuka, Ryo, Ogawa, Mamoru, Shimizu, Eisuke, Sato, Yasunori, Pflugfelder, Stephen, and Tsubota, Kazuo

Published
2022
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31. TFOS DEWS II pain and sensation report

Author

Belmonte, Carlos, Nichols, Jason J, Cox, Stephanie M, Brock, James A, Begley, Carolyn G, Bereiter, David A, Dartt, Darlene A, Galor, Anat, Hamrah, Pedram, Ivanusic, Jason J, Jacobs, Deborah S, McNamara, Nancy A, Rosenblatt, Mark I, Stapleton, Fiona, and Wolffsohn, James S

Subjects
Neurodegenerative, Pain Research, Chronic Pain, Neurosciences, Eye Disease and Disorders of Vision, Peripheral Neuropathy, Aetiology, Underpinning research, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Eye, Neurological, Animals, Cornea, Dry Eye Syndromes, Nociceptors, Pain, Sensation, Thermoreceptors, Neuropathic pain, Dry eye disease, Ocular surface dryness, Peripheral sensory nerves, TRP channels, Cold receptors, Polymodal nociceptors, Mechano-nociceptors, Trigeminal brainstem nuclear complex, Central nervous system, Corneal esthesiometry, In vivo confocal microscopy, In vivo confocal microscopy, Opthalmology and Optometry, Ophthalmology & Optometry
Abstract

Pain associated with mechanical, chemical, and thermal heat stimulation of the ocular surface is mediated by trigeminal ganglion neurons, while cold thermoreceptors detect wetness and reflexly maintain basal tear production and blinking rate. These neurons project into two regions of the trigeminal brain stem nuclear complex: ViVc, activated by changes in the moisture of the ocular surface and VcC1, mediating sensory-discriminative aspects of ocular pain and reflex blinking. ViVc ocular neurons project to brain regions that control lacrimation and spontaneous blinking and to the sensory thalamus. Secretion of the main lacrimal gland is regulated dominantly by autonomic parasympathetic nerves, reflexly activated by eye surface sensory nerves. These also evoke goblet cell secretion through unidentified efferent fibers. Neural pathways involved in the regulation of meibomian gland secretion or mucin release have not been identified. In dry eye disease, reduced tear secretion leads to inflammation and peripheral nerve damage. Inflammation causes sensitization of polymodal and mechano-nociceptor nerve endings and an abnormal increase in cold thermoreceptor activity, altogether evoking dryness sensations and pain. Long-term inflammation and nerve injury alter gene expression of ion channels and receptors at terminals and cell bodies of trigeminal ganglion and brainstem neurons, changing their excitability, connectivity and impulse firing. Perpetuation of molecular, structural and functional disturbances in ocular sensory pathways ultimately leads to dysestesias and neuropathic pain referred to the eye surface. Pain can be assessed with a variety of questionaires while the status of corneal nerves is evaluated with esthesiometry and with in vivo confocal microscopy.

Published
2017

36. Cell Type-Specific Extracellular Vesicles and Their Impact on Health and Disease

Author

Amin, Sohil, primary, Massoumi, Hamed, additional, Tewari, Deepshikha, additional, Roy, Arnab, additional, Chaudhuri, Madhurima, additional, Jazayerli, Cedra, additional, Krishan, Abhi, additional, Singh, Mannat, additional, Soleimani, Mohammad, additional, Karaca, Emine E., additional, Mirzaei, Arash, additional, Guaiquil, Victor H., additional, Rosenblatt, Mark I., additional, Djalilian, Ali R., additional, and Jalilian, Elmira, additional

Published
2024
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39. Anti-Infective and Anti-Inflammatory Pharmacotherapies

Author

Rosenblatt, Mark I., Barrett, James E., Editor-in-chief, Flockerzi, Veit, Series editor, Frohman, Michael A., Series editor, Geppetti, Pierangelo, Series editor, Hofmann, Franz B., Series editor, Michel, Martin C., Series editor, Page, Clive P, Series editor, Rosenthal, Walter, Series editor, Wang, KeWei, Series editor, Whitcup, Scott M., editor, and Azar, Dimitri T., editor

Published
2017
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40. End binding-3 inhibitor activates regenerative program in age-related macular degeneration

Author

Lee, Quinn, primary, Chan, Wan Ching, additional, Qu, Xinyan, additional, Sun, Ying, additional, Abdelkarim, Hazem, additional, Le, Jonathan, additional, Saqib, Uzma, additional, Sun, Mitchell Y., additional, Kruse, Kevin, additional, Banerjee, Avik, additional, Hitchinson, Ben, additional, Geyer, Melissa, additional, Huang, Fei, additional, Guaiquil, Victor, additional, Mutso, Amelia A., additional, Sanders, Martin, additional, Rosenblatt, Mark I., additional, Maienschein-Cline, Mark, additional, Lawrence, Matthew S., additional, Gaponenko, Vadim, additional, Malik, Asrar B., additional, and Komarova, Yulia A., additional

Published
2023
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46. Extracellular-Vesicle-Based Therapeutics in Neuro-Ophthalmic Disorders

Author

Massoumi, Hamed, Amin, Sohil, Soleimani, Mohammad, Momenaei, Bita, Ashraf, Mohammad Javad, Guaiquil, Victor H, Hematti, Peiman, Rosenblatt, Mark I, Djalilian, Ali R, Jalilian, Elmira, Massoumi, Hamed, Amin, Sohil, Soleimani, Mohammad, Momenaei, Bita, Ashraf, Mohammad Javad, Guaiquil, Victor H, Hematti, Peiman, Rosenblatt, Mark I, Djalilian, Ali R, and Jalilian, Elmira

Abstract

Extracellular vesicles (EVs) have been recognized as promising candidates for developing novel therapeutics for a wide range of pathologies, including ocular disorders, due to their ability to deliver a diverse array of bioactive molecules, including proteins, lipids, and nucleic acids, to recipient cells. Recent studies have shown that EVs derived from various cell types, including mesenchymal stromal cells (MSCs), retinal pigment epithelium cells, and endothelial cells, have therapeutic potential in ocular disorders, such as corneal injury and diabetic retinopathy. EVs exert their effects through various mechanisms, including promoting cell survival, reducing inflammation, and inducing tissue regeneration. Furthermore, EVs have shown promise in promoting nerve regeneration in ocular diseases. In particular, EVs derived from MSCs have been demonstrated to promote axonal regeneration and functional recovery in various animal models of optic nerve injury and glaucoma. EVs contain various neurotrophic factors and cytokines that can enhance neuronal survival and regeneration, promote angiogenesis, and modulate inflammation in the retina and optic nerve. Additionally, in experimental models, the application of EVs as a delivery platform for therapeutic molecules has revealed great promise in the treatment of ocular disorders. However, the clinical translation of EV-based therapies faces several challenges, and further preclinical and clinical studies are needed to fully explore the therapeutic potential of EVs in ocular disorders and to address the challenges for their successful clinical translation. In this review, we will provide an overview of different types of EVs and their cargo, as well as the techniques used for their isolation and characterization. We will then review the preclinical and clinical studies that have explored the role of EVs in the treatment of ocular disorders, highlighting their therapeutic potential and the challenges that need to be addressed

Published
2023

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