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2 results on '"Rossella Migliorino"'

1. Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors

Author

Stefano Cinti, Stefano Tomassi, Chiara Ciardiello, Rossella Migliorino, Marinella Pirozzi, Alessandra Leone, Elena Di Gennaro, Virginia Campani, Giuseppe De Rosa, Vincenzo Maria D’Amore, Salvatore Di Maro, Greta Donati, Sima Singh, Ada Raucci, Francesco Saverio Di Leva, Horst Kessler, Alfredo Budillon, and Luciana Marinelli

Subjects
Chemistry, QD1-999
Abstract

Abstract Despite progress in the prevention and diagnosis of cancer, current technologies for tumor detection present several limitations including invasiveness, toxicity, inaccuracy, lengthy testing duration and high cost. Therefore, innovative diagnostic techniques that integrate knowledge from biology, oncology, medicinal and analytical chemistry are now quickly emerging in the attempt to address these issues. Following this approach, here we developed a paper-based electrochemical device for detecting cancer-derived Small Extracellular Vesicles (S-EVs) in fluids. S-EVs were obtained from cancer cell lines known to express, at a different level, the αvβ6 integrin receptor, a well-established hallmark of numerous epithelial cancer types. The resulting biosensor turned out to recognize αvβ6-containing S-EVs down to a limit of 0.7*103 S-EVs/mL with a linear range up to 105 S-EVs /mL, and a relative standard deviation of 11%, thus it may represent a novel opportunity for αvβ6 expressing cancers detection.

Published
2024
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2. Large extracellular vesicles: Size matters in tumor progression

Author

Chiara Ciardiello, Alfredo Budillon, Rossella Migliorino, and Alessandra Leone

Subjects
0301 basic medicine, Endocrinology, Diabetes and Metabolism, media_common.quotation_subject, Immunology, Exosomes, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Extracellular Vesicles, Mice, 0302 clinical medicine, Neoplasms, Biomarkers, Tumor, Immunology and Allergy, Animals, Humans, Internalization, Lipid bilayer, media_common, Chemistry, Cell Membrane, Microvesicles, Cell biology, 030104 developmental biology, Cytoplasm, Tumor progression, 030220 oncology & carcinogenesis, Cancer cell, Organelle Size, Disease Progression, Cancer biomarkers, Biogenesis
Abstract

Extracellular Vesicles (EVs) represent a heterogeneous population of particles naturally released from all cells, delimited by a lipid bilayer and able to horizontally transfer their cargos to recipient cells. These features imply the growing interest on EVs in cancer biology as biomarkers and therapeutic targets. In this review, we will highlight the specific process related to biogenesis and release of large EVs (L-EVs) derived from the plasma membrane (PM) compared to the small and well described exosomes, generated through the classical endosome-multivesicular body (MVB) pathway. The control of PM rigidity by cells depends on lipid/protein composition, cytoskeleton dynamics, cytoplasmic viscosity, ions balance, metabolic reprogramming and specific intracellular signaling pathways, all critical determinants of L-EVs biogenesis. We will focus in details on a specific class of L-EVs, named Large Oncosomes (LO), exclusively shed by cancer cells and with a size ranging from 1 μm up to 10 μm. We will examine LO specific cargos, either proteins or nucleic acids (i.e. mRNA, microRNAs, single/double-stranded DNA), as well as their functional role in cancer development and progression, also discussing the mechanisms of L-EVs internalization by recipient cells. Overall we will highlight the potential of LO as specific diagnostic/prognostic cancer biomarkers discussing the associated challenges.

Published
2019

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