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Research has extensively shown that most people who experience maltreatment in their childhood develop mental disorders, psychosocial adjustment problems, and, in many cases, become maltreating adults themselves. Preventing child maltreatment and treating abused children and abusive parents are, therefore, pressing public health issues. As established by the UK Children Act in 1989, child development is enhanced by remaining in the family whenever the child's safety is assured. Thus, developing prevention and intervention programs for the purpose of repairing, whenever possible, the child-parent relationship should be a social priority. This narrative review focuses on the psychoanalytic studies related to intrapsychic dynamics and therapeutic intervention for physically abusive parents. The role of the transgenerational transmission of abuse and parents' narcissistic fragility is crucial. Psychoanalytic interventions focus on helping the parent work through their past painful experiences and narcissistic vulnerability. Parent-child psychotherapy and mentalization-based treatment have been found to be prevalent, while there is scarce literature regarding intensive individual psychoanalytic treatment. Within the framework of attachment theory, brief interventions were developed; however, they did not prove effective for those parents who suffered experiences of maltreatment or severe neglect in childhood and for whom long-term parent-child psychotherapy resulted, which proved to be the most effective.

Mentalization is considered an essential ability for social cognition as well as a crucial competency in parenting to further the development of internal structures that are decisive for self organization and affect regulation in children. Yet, few empirical studies have investigated whether, and to what extent, parents who maltreat their children poorly mentalize. The aim of this research was to study the mentalization ability in a group of parents who maltreated their children and had been referred by the Courts for Child Custody and Parenting Plan Evaluation (Group 1), and in a comparison, non-clinical group of parents (Group 2). Adult Attachment Interview (AAI), rated in terms of both the Berkeley AAI System and the Reflective Functioning Scale was administered. Group 1 had severely impaired reflective functioning (RF) in 83.3% of cases, whilst impaired RF was found in only 12.5% of Group 2 parents. For the most part, parents in Group 1 showed Negative Reflective Functioning, systematically resisting taking a reflective stance, and the parents who most severely maltreated their children showed distorted and/or self-serving passages associated with a particular type of dismissing pattern of attachment (DS2) based on the derogation of attachment. The frequent occurrence of derogation in these parents likely explains how much the devaluation of relationships and attachment needs, presumably acquired during childhood with defensive purposes and in order to exclude the pain and perception of emotional weakness from awareness, hinders the capacity to care for children in the full respect of their needs., Competing Interests: The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Rosso.)

Although the Rorschach is the most widely studied and used non self-report measure to assess personality, no empirical studies have been carried out to investigate what level of expertise is needed to properly collect valid Rorschach protocols. The aim of the current study was to explore, using a within-subject design, the effect of training on proficiency in administering the Rorschach, specifically a) what kind of errors are associated with different levels of training, and b) what level of expertise guarantees a sufficiently effective Rorschach administration. The second (T1), the fifth (T2) and the tenth (T3) Rorschach protocols collected by 50 licensed psychologists attending a Rorschach training course were examined to explore what kind of mistakes were made at the different stages of training. In the Response phase, examiners collected more simplistic protocols and made more errors at T1, while they showed satisfactory proficiency at T2. Regarding the Inquiry Phase, the most relevant finding was that at T1 examiners made both errors of omission and commission, while at T2 they no longer made errors of omission but they did make more errors of commission, likely as a temporary effect of the training. At T3 a sufficiently effective Rorschach administration was carried out. Findings suggest that a 100-h Rorschach training course and 10 administrations as practicum, each followed by individual feedback and group discussion, might be sufficient to guarantee sufficiently effective Rorschach administration skills., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Previous studies have reported a significant positive association between ability emotional intelligence (EI) and attachment security. However, these studies may, to some extent, be misleading because they relied on self-report measures of attachment security. Furthermore, to our knowledge, no study has yet investigated the relationship between ability EI and mentalization, operazionalized as reflective functioning (RF), although EI and RF were assumed to be "conceptual cousins." In an attempt to overcome some of the limitations of the previous research, the current study investigated the relationships between ability EI, attachment security, and mentalization measured via the Adult Attachment Interview (AAI). Ninety-three volunteer participants from an Italian community population (49.5% males), aged from 27 to 55 years ( M = 39.44; SD = 6.84), took part in the study. Significant positive correlations were found between ability EI, attachment security, and RF. The results shed some light on the relationship between different attachment strategies and ability EI. Hyperactivating attachment strategies correlated negatively with ability EI, while the correlation between ability EI and attachment deactivating strategies depend on which defensive strategy is used: avoiding the painful emotional contact with the memory of unpleasant childhood attachment experiences positively correlated with experiential EI, whilst the resort to derogation of the attachment needs correlated with impairment in EI. Findings from the current study suggest that future studies in developmental psychology are needed to investigate the development of the ability EI in relation to the quality of the attachment models more in-depth., Competing Interests: The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Rosso.)

In recent years, many attempts have been made to find new plant proteases to make artisan cheeses. The global increase in cheese consumption, together with a lower supply and increasing cost of calf rennet, religious factors (Islam and Judaism) and food choices (vegetarianism) have led to the search for suitable rennet substitutes for milk clotting. This study describes the milk-clotting and hydrolytic activities of an aspartic protease from Salpichroa origanifolia fruits (SoAP) on individual caseins to explore its potential use as an alternative to animal rennet. The milk-clotting index obtained for SoAP was 8.4 times lower than that obtained for chymosin. SoAP showed a higher degree of hydrolysis on α-casein than on the other fractions under the proposed conditions. RP-HPLC, mass spectrometry analyses and sequencing of the hydrolysates allowed identifying five peptides from α-casein, one peptide from β-casein, and three peptides from k-casein. In silico analysis showed that the peptides identified may display a wide variety of potential biological activities. These results demonstrate the possibility of using SoAP for the manufacture of new types or artisan cheeses, with the simultaneous added value of the potential health-promoting benefits of the bioactive peptides generated during the hydrolysis., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The current study investigated the inter-rater reliability and the construct validity of the Rorschach Lerner Defense Scale (LDS). In particular, it aimed to explore the inter-rater reliability, analyzing the most frequent coding mistakes in an attempt to improve the coding guidelines, and to investigate the ability of the scale to distinguish between individuals with neurotic-level and borderline-level personality organization, according to the Psychodynamic Diagnostic Manual-2 (PDM-2), and non-clinical subjects. Eighty clinical subjects and 80 non-clinical ones participated in the study. Among the clinical subjects, 40 have borderline-level personality organization and 40 have neurotic-level personality organization. Non-clinical subjects were drawn from an archival dataset of non-clinical individuals who previously participated in a Rorschach normative study. The LDS showed substantial inter-rater reliability; however, guidelines could be improved, specifically with regard to the threshold for coding Devaluation and Idealization at level 1. Furthermore, more examples should be included in the manual about the coding of Projective Identification and Denial. The LDS distinguished borderline-level subjects from both the non-clinical and neurotic groups with regard to Devaluation and Projective Identification, with borderline-level personality organization subjects reporting higher scores than either of the two other groups. Only the Denial scale discriminated between the non-clinical and neurotic group, with the latter reporting higher scores of high-level Denial., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Rosso, Airaldi and Camoirano.)

Alexandria was both an important city and a place of inspiration in the history of medicine since the 3rd century BC until 7th century AD. Its fertile intellectual and scientific environment gave rise to a radical and new thinking and understanding of the disease process. King Ptolemy I (323-285 BC) established the Museion and the Great Library of Alexandria fabled in Antiquity for its treasures of wisdom. This complex interaction between Greeks and Egyptians before and under the Ptolemies gave scientists an opportunity to live and work under royal patronage with excellent repercussions. Scholars were attracted to take part in scientific research, especially in medicine and mathematics, developing different known areas and programs. Furthermore scholars in Alexandria also brought back new ideas and activities each with specialized knowledge of the subjects. As the medical science of ancient Greece plunged into political decadence and setbacks - scientific work was renewed in this city with the arriving of the greatest minds of the day. The 'Faculty' of Medicine in Alexandria seems to be an integral part of the Mouseion. In the 3rd century BC, the Ptolemaic capital caught the different trends and became the seat of divergent and independent schools. The debt of the Alexandrian physicians to the native Egyptian doctors, highly specialized, was very considerable. Coan school in this time had general professionals who recommended therapeutic and nutricional strategies and diets while Alexandria science provided academic specialization. On the other hand the court physicians, involved in high politics, could be medical specialists meanwhile the teachers of advanced medicine were sometimes left undisturbed in the pursuit of their research. Being able to commit poisoning crimes with drugs by political interests, they were scared of them. Animal and plants toxins became an accepted and divulged study, very fashionable, and Alexandrian school developed scientific research in iology, name given later to this science. Greek sources on toxicology became a special and developed branch in medical literature and from Apollodorus of Tarentum onwards, the father of iology and his book Peri thêriōn, the interest in Greek treatises spread., Competing Interests: The authors report no declarations of interest., (© 2021 The Author(s).)

A number of tasks have been developed to measure the affective theory of mind (ToM), nevertheless, recent studies found that different affective ToM tasks do not correlate with each other, suggesting that further studies on affective ToM and its measurement are needed. More in-depth knowledge of the tools that are available to assess affective ToM is needed to decide which should be used in research and in clinical practice, and how to interpret results. The current study focuses on the Reading the Mind in the Eyes Test (RMET) primarily to investigate in a sample of 112 children the currently unexplored relationships in middle childhood between performance on the RMET and fluid intelligence. Relationships with receptive vocabulary, age, and sex were also investigated. Moreover, because studying the family's influence on children mentalization could have important implications in developing prevention and treatment interventions, this study offers a novel contribution to the field by exploring the family's influence on children's RMET performance. Although significant positive correlations were found among RMET-C performance, fluid intelligence, and receptive language, regression analysis revealed that fluid intelligence was the only predictor. No family influence was found on children's RMET performance. On the whole, results from the current study offer some support to the hypothesis that RMET-C is not a "pure" ToM task, specifically the effect of fluid intelligence on RMET performance should be taken into account when RMET is used both in research and in the clinical setting., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Rosso and Riolfo.)

Chia expeller is a by-product of the extrusion process of chia seeds generated during oil production. Typically, this material is non-utilized or used for non-valuable applications. In the present work, the chia expeller was hydrolysed with Papain and the antioxidant properties of the resultant peptides were evaluated. Papain treatment of the chia seed expeller demonstrated an enrichment of low molecular weight peptides (molecular weight <15 kDa) as determined by SDS-PAGE and MALDI-TOF/MS analyses. Such peptides showed a potent radical scavenging effect in vitro against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals in comparison with those non-hydrolysed samples. Taken together our results demonstrate the generation of functional peptides from the chia expeller by enzymatic hydrolysis with Papain. This value-added hydrolysate can be potentially included as a supplement in functional food and nutraceutical products., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Plant proteases play a fundamental role in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases (AP) are expressed in different plant organs and have antimicrobial activity. Previously, we purified an AP from Salpichroa origanifolia fruits called salpichroin. The aim of this work was to determine the cytotoxic activity of this enzyme on selected plant and human pathogens. For this purpose, the growth of the selected pathogens was analysed after exposure to different concentrations of salpichroin. The results showed that the enzyme was capable of inhibiting Fusarium solani and Staphylococcus aureus in a dose-dependent manner. It was determined that 1·2 μmol l -1 of salpichroin was necessary to inhibit 50% of conidial germination, and the minimal bactericidal concentration was between 1·9 and 2·5 μmol l -1 . Using SYTOX Green dye we were able to demonstrate that salpichroin cause membrane permeabilization. Moreover, the enzyme treated with its specific inhibitor pepstatin A did not lose its antibacterial activity. This finding demonstrates that the cytotoxic activity of salpichroin is due to the alteration of the cell plasma membrane barrier but not due to its proteolytic activity. Antimicrobial activity of the AP could represent a potential alternative for the control of pathogens that affect humans or crops of economic interest., Significance and Impact of the Study: This study provides insights into the antimicrobial activity of an aspartic protease isolated from Salpichroa origanifolia fruits on plant and human pathogens. The proteinase inhibited Fusarium solani and Staphylococcus aureus in a dose-dependent manner due to the alteration of the cell plasma membrane barrier but not due to its proteolytic activity. Antimicrobial activity of salpichroin suggests its potential applications as an important tool for the control of pathogenic micro-organisms affecting humans and crops of economic interest. Therefore, it would represent a new alternative to avoid the problems of environmental pollution and antimicrobial resistance., (© 2018 The Society for Applied Microbiology.)

An aspartic protease from Salpichroa origanifolia fruits was successfully immobilized onto an activated support of glutaraldehyde agarose. The immobilized enzyme presented higher thermal stability than the free enzyme from 40°C to 50°C and high reusability, retaining 54% of the initial activity after ten cycles of the process. Whey protein concentrates (WPC) were hydrolyzed with both free and immobilized enzyme, reaching a similar degree of hydrolysis of approximately 6-8% after 20h. In addition, the immobilized derivate hydrolyzed α-lactalbumin protein with a higher affinity than β-lactoglobulin. The hydrolysate was ultra-filtrated, and the fractions were evaluated for antioxidant activities with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity method. The fraction containing peptides with a molecular mass below 3kDa demonstrated a strong radical quenching effect (IC 50: 0.48mg/ml). These results suggest that hydrolyzed WPC could be considered as a promising source of natural food antioxidants for the development of functional food., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

The present study investigated whether, and to what extent, reflective functioning (RF) during preadolescence is associated with maternal attachment security and RF, and with the child's attachment security. Thirty-nine mother-preadolescent child dyads from a non-clinical population participated in the study. Maternal and child RF were assessed by applying the Reflective Functioning Scale to the Adult Attachment Interview (AAI) and to the Child Attachment Interview transcripts. Children of mothers who showed a secure attachment model regarding the relationship with their parents during childhood reported higher levels of RF than the children of mothers who were classified as insecure on the AAI. Child RF was positively associated with maternal "Coherence of the Mind" on the AAI and negatively associated with maternal derogation of attachment. A strong, significant association was also found between child attachment security and child RF. Children who were rated as being more emotionally open, more able to balance positive and negative descriptions of their parents, more prone to support their assertions through examples, and more able to positively resolve conflicts with their parents showed higher RF. On the contrary, children who resorted to a higher extent to idealization and dismissal toward their parents showed a lesser degree of RF. Notably, a very strong association was found between the score on the "Overall coherence" subscale and the child's ability to mentalize mixed-ambivalent mental states in the context of their family relationships. As expected, child and maternal RF resulted significantly positively correlated with each other. In particular, only maternal RF (and not maternal attachment security) predicted child RF, and only maternal ability to mentalize mixed-ambivalent mental states predicted the corresponding ability in the children.

In Ancient times, an active trade of exotic and peculiar drugs tool place along the Silk Road. Coming through China, India, Central Asia, Armenia, including Colchis, Arabia, Nubia as far as Greece and Rome, it was centered during Ptolemaic and Roman times in Alexandria, the world Emporium, remarkably advanced in scientific medicine. Physicians required a variety of active ingredients for their pharmacotherapy, following various related branches of medicine. These included: 1) herbal remedies: including toxic plants 2) polypharmacy: missing together all kind of drugs 3) dreckapotheke or copropharmacy, employing unclean materials 4) organic therapy, using exotic or domestic animal products 5) aromatherapy, lined to essential oils and perfumes 6) 'medical astrology and botany', regarding the laws of sympathy in the natural world 7) alchemy and magic medicine: with occult knowledge

This study investigated the impact of maternal reflective functioning (RF) and attachment security on children's mentalization. The Adult Attachment Interview (AAI) was administered to mothers in a sample of 41 mother-preadolescent dyads. AAI transcripts were rated in terms of the Berkeley AAI System (Main and Goldwyn, 1998) and the Reflective Functioning Scale (RFS; Fonagy et al., 1998). Preadolescent mentalization was assessed using a semi-structured interview adapted from O'Connor and Hirsch (1999) and also by analyzing mental-state talk produced during an autobiographical interview. Relationships between maternal RF and children's mentalization were analyzed, with consideration given to the different RFS markers and references to positive, negative, and mixed-ambivalent mental states. Children's mentalization was positively correlated with the mother's RF, particularly the mother's ability to mentalize negative or mixed-ambivalent mental states. No significant differences in mentalization were observed between children of secure and insecure mothers.

In this study, three different subtypes of Space responses to the Rorschach test were hypothesized: S-fusion, S-reversal, and S-integration. The relationship between these subtypes and feelings of anger and aggression was investigated. The Rorschach test, the State-Trait Anxiety Inventory-2 (STAXI-2), and the Aggression Questionnaire (AQ) were administered to 50 university students. Scores on the STAXI-2 were positively associated with S-fusion and negatively associated with S-integration. No significant associations of S subtypes with aggression were found. The findings support the hypothesis that different figure-ground relationships, shown in the subtypes of S responses, indicate different psychological processes.

This report describes the purification of an aspartic protease (salpichroin) from ripe fruits of Salpichroa origanifolia (Solanaceae) starting with precipitation using organic solvents and anionexchange chromatography with 32.1% recovery and 13.4-fold purification. SDS-PAGE and zymograms of this enzyme showed a single band corresponding to an apparent molecular mass of approximately 32 kDa. The biochemical and kinetic characterization of the pure enzyme showed an acidic behavior with an optimal pH value around 3.0-4.5 with hemoglobin and 5.5-6.0 with casein. Salpichroin activity was inhibited by pepstatin but not by phenylmethylsulfonyl fluoride, E-64, EDTA or 1,10-phenanthroline, thus suggesting an aspartic protease behavior. Salpichroin hydrolyzed natural substrates, such as casein and hemoglobin, with high specific activity. Kinetic studies conducted with the synthetic peptide H-Pro- Thr-Glu-Phe-p-(NO2)-Phe-Arg-Leu-OH showed lower affinity (Km 494 µM) than other representative aspartic proteases. By investigating the cleavage of oxidized insulin β-chain to establish the hydrolytic specificity of salpichroin, we found six cleavage sites on the substrate of peptide bonds similar to those of chymosin. MALDI-TOF/TOF-MS of the tryptic ingel digest of salpichroin showed that the isolated protease shared homologous sequences with other plant proteases of the A1 aspartic protease family. This is the first report concerning the isolation and biochemical characterization of an aspartic protease isolated from Salpichroa origanifolia fruits.

In the Ancient and New World there was a custom to preserve the corpse in a natural and artificial way. Since Paleolithic man believed in an afterlife and even in Mesoamerica and the Andes cultures, care and ceremony were practiced to the burial of the dead in an ancestral cult. Mortuary rituals were developed in Pre-dynastic Egypt (4500-3100 BC) but apparently they had begun before in America, c. 5000 BC. Mummies served for assisting the soul to survive and for preventing the dead from frightening the livings. Incas arrived at a point of perfection in these practices after other Andean cultures but we should not forget their older predecessors, the Chinchorro culture on the arid coast of the Atacama Desert. Different steps in the technique can be distinguished in both worlds: natural desiccation covered by animal skins, methods to protect the body skin and flesh removal, replacement with clay; black, red or mud-coated corpses, evisceration, body cavity treatment, cleansing and anointing the interior, brain removal, mummified bodies, corpses covered with natron, before being washed and bandaged or wrapped. It will be necessary to carefully check dates, techniques and periods in the two zones to establish exactly the evolution of the methods applied.

Different types of alcoholic beverages such as wine and beer were used in ancient times for various medicinal purposes. Being the oldest and probably the most widely used drugs, they were known to have some therapeutic value, in addition to the vital part they played in the daily life of people. Ethanol is produced by fermentation of a variety of plants and consumed either in a diluted form or concentrated by distillation to concoct alcoholic beverages. Beer made of fermented barley is an alcoholic drink that was believed to contain a spirit or a god. It is a drink of relatively low alcohol content with supernatural properties. The same was believed for wine. Considered to be divine, these beverages were the long sought elixirs of life and appeared in religious ceremonies, in mythology, and in social meals, such as the Greek symposia. In addition, these alcoholic drinks were considered to be a remedy for practically every disease and, therefore, were a common ingredient in ancient prescriptions. They were used as anaesthetics that dull the pain, as stimulants, as analgesics, as antiseptics to cleanse wounds and relieve pain, as emetics, as digestives, as antidotes for plant poisoning, for bites and stings, and as purifiers. However, we should not overlook the harmful effects of alcohol abuse such as drunkenness, chronic liver disease and, in modern terminology, infirmities that included pancreatitis, cardiomyopathy, peripheral neuropathy, dementia, and central nervous system disorders.

Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 degrees C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 degrees C and pH 6.4. After 4h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of alpha-CD:beta-CD:gamma-CD was 1.00:0.70:0.16.

We suggest a checklist for inspection in banks. The checklist is composed of 14 schedules where information on bank clerks and buildings is collected. Principle risk factors are analysed (workplaces, lighting and noise, air quality, emergency management). The critical points are observed and the times of intervention are established. Then follows data processing and the reports are transmitted to the employer. The checklist, tested on 250 workplaces, allows us to identify the interventions having priority to be realized through appropriate planning. A quantitative evaluation of the risk is matched with immediate and brief operative indications. The situations to improve are: cleanliness of workplaces, thermal comfort, electricity/telephone wires. The checklist seems to be a valid instrument for the evaluation of risk factors, their management and times of intervention. Such instrument simplifies, moreover, the employer choices of intervention.

BACKGROUND: The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related alpha-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced. In addition, CGTase production is highly dependent on the strain, medium composition and culture conditions. Therefore we undertook this study with a newly isolated strain of Bacillus circulans. RESULTS: CGTase activity produced from Bacillus circulans DF 9R was optimised in shake flasks using a combination of conventional sequential techniques and statistical experimental design. Effects of nutrients, including several carbon, nitrogen and mineral sources, were assayed. The selected minimal medium consisted of 1.5 % cassava starch, 0.4 % ammonium sulphate, 0.1 M phosphate buffer, 0.002 % MgSO4 and 0.002 % FeSO4. The optimal concentrations of carbon and nitrogen sources were determined using a central composite design. Maximum CGTase activity obtained in supernatants was 5.8 U/mL in 48 h of incubation. Optimal conditions for enzyme production also included an initial pH of 8.3 and 37 degrees C as the incubation temperature.Cell growth and CGTase production profile were not linked to each other, suggesting that enzyme production/secretion is not growth-associated but mainly a late-log phase event. CONCLUSION: We have screened conditions for optimal CGTase production. The selected minimal medium contained starch, ammonium, Mg2+ and Fe2+ as essential nutrients. As an additional advantage, this medium does not require complex nitrogen sources with varying and unknown composition.

From wild type inoculum of Bacillus circulans DF 9 which produce cyclomaltodextrin-glucanotransferase (EC 2.4.1.19, CGTase), two different kinds of colonies were isolated, which correspond to the classical S-R variation. From the culture medium of both colonies grown together a CGTase was purified about 50 fold with a yield of 54% in two steps. From pure R-cell culture the enzyme was purified by about 38 folds with a yield of 79% in only one step, showing a complete homogeneity as judged by a native PAGE analysis.

The cyclomaltodextrin-glucanotransferase (EC2.4.1.19, CGTase) which was purified to homogeneity from Bacillus circulans strain DF 9, R type, showed a pI of 5.3 determined by disc-isoelectric focusing, a Mw of 78 kDa estimated by SDS-PAGE with a range of pH of optimal enzymatic activity rather large (4.5-7.5). The thermal stability of the enzyme at 55 degrees C was increased 4-5 times when calcium ion (10 to 100 mM) or alpha-cyclodextrins (10 mM) were added to the preincubation mixtures. The alpha: beta: gamma ratio determined by HPLC was about 1:0.9:0.4 and the maximal conversion to cyclodextrins with 5% soluble starch was about 36%.