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146 results on '"Roudbaraki, Morad"'

1. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence

Author

Farfariello, Valerio, Gordienko, Dmitri V., Mesilmany, Lina, Touil, Yasmine, Germain, Emmanuelle, Fliniaux, Ingrid, Desruelles, Emilie, Gkika, Dimitra, Roudbaraki, Morad, Shapovalov, George, Noyer, Lucile, Lebas, Mathilde, Allart, Laurent, Zienthal-Gelus, Nathalie, Iamshanova, Oksana, Bonardi, Franck, Figeac, Martin, Laine, William, Kluza, Jerome, Marchetti, Philippe, Quesnel, Bruno, Metzger, Daniel, Bernard, David, Parys, Jan B., Lemonnier, Loïc, and Prevarskaya, Natalia

Published
2022
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2. Association of future cancer metastases with fibroblast activation protein-α: a systematic review and meta-analysis

Author

Janani, Majid, primary, Poorkhani, Amirhoushang, additional, Amiriani, Taghi, additional, Donyadideh, Ghazaleh, additional, Ahmadi, Farahnazsadat, additional, Jorjanisorkhankalateh, Yalda, additional, Beheshti-Nia, Fereshteh, additional, Kalaei, Zahra, additional, Roudbaraki, Morad, additional, Soltani, Mahsa, additional, Khori, Vahid, additional, and Alizadeh, Ali Mohammad, additional

Published
2024
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4. Association of future cancer metastases with fibroblast activation protein-a: a systematic review and meta-analysis.

Author

Janani, Majid, Poorkhani, Amirhoushang, Amiriani, Taghi, Donyadideh, Ghazaleh, Ahmadi, Farahnazsadat, Jorjanisorkhankalateh, Yalda, Beheshti-Nia, Fereshteh, Kalaei, Zahra, Roudbaraki, Morad, Soltani, Mahsa, Khori, Vahid, and Alizadeh, Ali Mohammad

Subjects
METASTASIS, LYMPHATIC metastasis, FIBROBLASTS, ONLINE databases, BLOOD vessels
Abstract

Introduction: Fibroblast activation protein-a (FAP-a) is a vital surface marker of cancer-associated fibroblasts, and its high expression is associated with a higher tumor grade and metastasis. A systematic review and a meta-analysis were performed to associate future metastasis with FAP-a expression in cancer. Methods: In our meta-analysis, relevant studies published before 20 February 2024 were systematically searched through online databases that included PubMed, Scopus, and Web of Science. The association between FAP-a expression and metastasis, including distant metastasis, lymph node metastasis, blood vessel invasion, vascular invasion, and neural invasion, was evaluated. A pooled odds ratio (OR) with 95% confidence intervals (CI) was reported as the measure of association. Results: A total of 28meta-analysis. The random-effects model for five parameters showed that a high FAP-a expression was associated with blood vessel invasion (OR: 3.04, 95% CI: 1.54–5.99, I² = 63%, P = 0.001), lymphovascular invasion (OR: 3.56, 95% CI: 2.14–5.93, I ² = 0.00%, P < 0.001), lymph node metastasis (OR: 2.73, 95% CI: 1.96–3.81, I² = 65%, P < 0.001), and distant metastasis (OR: 2.59; 95% CI: 1.16–5.79, I² = 81%, P < 0.001). However, our analysis showed no statistically significant association between high FAP-a expression and neural invasion (OR: 1.57, 95% CI: 0.84–2.93, I² = 38%, P = 0.161). Conclusions: This meta-analysis indicated that cancer cells with a high FAP-a expression have a higher risk of metastasis than those with a low FAP-a expression. These findings support the potential importance of FAP-a as a biomarker for cancer metastasis prediction. [ABSTRACT FROM AUTHOR]

Published
2024
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7. A systematic method introduced a common lncRNA-miRNA-mRNA network in the different stages of prostate cancer

Author

Vahabzadeh, Gelareh, primary, Khalighfard, Solmaz, additional, Alizadeh, Ali Mohammad, additional, Yaghobinejad, Mahsa, additional, Mardani, Mahta, additional, Rastegar, Tayebeh, additional, Barati, Mahmood, additional, Roudbaraki, Morad, additional, Esmati, Ebrahim, additional, Babaei, Mohammad, additional, and Kazemian, Ali, additional

Published
2023
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8. Supplemental data from Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells

Author

Derouiche, Sandra, primary, Mariot, Pascal, primary, Warnier, Marine, primary, Vancauwenberghe, Eric, primary, Bidaux, Gabriel, primary, Gosset, Pierre, primary, Mauroy, Brigitte, primary, Bonnal, Jean-Louis, primary, Slomianny, Christian, primary, Delcourt, Philippe, primary, Dewailly, Etienne, primary, Prevarskaya, Natalia, primary, and Roudbaraki, Morad, primary

Published
2023
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9. Data from Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells

Author

Derouiche, Sandra, primary, Mariot, Pascal, primary, Warnier, Marine, primary, Vancauwenberghe, Eric, primary, Bidaux, Gabriel, primary, Gosset, Pierre, primary, Mauroy, Brigitte, primary, Bonnal, Jean-Louis, primary, Slomianny, Christian, primary, Delcourt, Philippe, primary, Dewailly, Etienne, primary, Prevarskaya, Natalia, primary, and Roudbaraki, Morad, primary

Published
2023
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10. A systematic method introduced a common lncRNA-miRNAmRNA network in the different stages of prostate cancer.

Author

Vahabzadeh, Gelareh, Khalighfard, Solmaz, Alizadeh, Ali Mohammad, Yaghobinejad, Mahsa, Mardani, Mahta, Rastegar, Tayebeh, Barati, Mahmood, Roudbaraki, Morad, Esmati, Ebrahim, Babaei, Mohammad, and Kazemian, Ali

Subjects
PROSTATE cancer, GENE expression, TUMOR classification, GLEASON grading system, DATABASES
Abstract

Introduction: The present study aimed to investigate the interaction of the common lncRNA-miRNA-mRNA network involved in signaling pathways in different stages of prostate cancer (PCa) by using bioinformatics and experimental methods. Methods: Seventy subjects included sixty PCa patients in Local, Locally Advanced, Biochemical Relapse, Metastatic, and Benign stages, and ten healthy subjects were entered into the current study. The mRNAs with significant expression differences were first found using the GEO database. The candidate hub genes were then identified by analyzing Cytohubba and MCODE software. Cytoscape, GO Term, and KEGG software determined hub genes and critical pathways. The expression of candidate lncRNAs, miRNAs, and mRNAs was then assessed using Real-Time PCR and ELISA techniques. Results: 4 lncRNAs, 5 miRNAs, and 15 common target genes were detected in PCa patients compared with the healthy group. Unlike the tumor suppressors, the expression levels of common onco-lncRNAs, oncomiRNAs, and oncogenes showed a considerable increase in patients with advanced stages; Biochemical Relapse and Metastatic, in comparison to the primary stages; Local and Locally Advanced. Additionally, their expression levels significantly increased with a higher Gleason score than a lower one. Conclusion: Identifying a common lncRNA-miRNA-mRNA network associated with prostate cancer may be clinically valuable as potential predictive biomarkers. They can also serve as novel therapeutic targets for PCa patients. [ABSTRACT FROM AUTHOR]

Published
2023
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12. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence.

Author

Farfariello, Valerio, Gordienko, Dmitri V., Mesilmany, Lina, Touil, Yasmine, Germain, Emmanuelle, Fliniaux, Ingrid, Desruelles, Emilie, Gkika, Dimitra, Roudbaraki, Morad, Shapovalov, George, Noyer, Lucile, Lebas, Mathilde, Allart, Laurent, Zienthal-Gelus, Nathalie, Iamshanova, Oksana, Bonardi, Franck, Figeac, Martin, Laine, William, Kluza, Jerome, and Marchetti, Philippe

Subjects
AGING, CANCER cell proliferation, STROMAL cells, CELLULAR aging, OXIDATIVE phosphorylation, OXYGEN consumption, PLANT growth
Abstract

Cellular senescence is implicated in a great number of diseases including cancer. Although alterations in mitochondrial metabolism were reported as senescence drivers, the underlying mechanisms remain elusive. We report the mechanism altering mitochondrial function and OXPHOS in stress-induced senescent fibroblasts. We demonstrate that TRPC3 protein, acting as a controller of mitochondrial Ca2+ load via negative regulation of IP3 receptor-mediated Ca2+ release, is down regulated in senescence regardless of the type of senescence inducer. This remodelling promotes cytosolic/mitochondrial Ca2+ oscillations and elevates mitochondrial Ca2+ load, mitochondrial oxygen consumption rate and oxidative phosphorylation. Re-expression of TRPC3 in senescent cells diminishes mitochondrial Ca2+ load and promotes escape from OIS-induced senescence. Cellular senescence evoked by TRPC3 downregulation in stromal cells displays a proinflammatory and tumour-promoting secretome that encourages cancer epithelial cell proliferation and tumour growth in vivo. Altogether, our results unravel the mechanism contributing to pro-tumour behaviour of senescent cells. Mitochondrial Ca2+ homeostasis is reported to influence cellular senescence. Here the authors show that TRPC3 limits senescence by inhibiting IP3R-mediated Ca2+ release and ER mitochondria Ca2+ transfer and that the downregulation of TRPC3 in stromal cells affects SASP production and tumour progression. [ABSTRACT FROM AUTHOR]

Published
2022
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16. Prostate cell differentiation status determines transient receptor potential melastatin member 8 channel subcellular localization and function

Author

Bidaux, Gabriel, Flourakis, Matthieu, Thebault, Stephanie, Zholos, Alexander, Beck, Benjamin, Gkika, Dimitra, Roudbaraki, Morad, Bonnal, Jean-Louis, Mauroy, Brigitte, Shuba, Yaroslav, Skryma, Roman, and Prevarskaya, Natalia

Subjects
Prostate cancer -- Development and progression, Tumor markers -- Research, Cell differentiation -- Health aspects, Cancer -- Research, Oncology, Experimental
Abstract

In recent years, the transient receptor potential melastatin member 8 (TRPM8) channel has emerged as a promising prognostic marker and putative therapeutic target in prostate cancer (PCa). However, the mechanisms [...]

Published
2007

17. Functional and molecular identification of intermediate-conductance [Ca.sup.2+]-activated [K.sup.+] channels in breast cancer cells: association with cell cycle progression

Author

Ouadid-Ahidouch, Halima, Roudbaraki, Morad, Delcourt, Philippe, Ahidouch, Ahmed, Joury, Nathalie, and Prevarskaya, Natalia

Subjects
Molecules -- Influence, Ion channels -- Influence, Ion channels -- Research, Cell cycle -- Research, Breast cancer -- Causes of, Breast cancer -- Research, Biological sciences
Abstract

We have previously reported that the hEAG [K.sup.+] channels are responsible for the potential membrane hyperpolarization that induces human breast cancer cell progression into the G1 phase of the cell cycle. In the present study, we evaluate the role and functional expression of the intermediate-conductance [Ca.sup.2+.sub.]-activated [K.sup.+] channel, hIK1-like, in controlling cell cycle progression. Our results demonstrate that hIK1 current density increased in cells synchronized at the end of the G1 or S phase compared with those in the early GI phase. This increased current density paralleled the enhancement in hIK1 mRNA levels and the highly negative membrane potential. Furthermore, in cells synchronized at the end of G1 or S phases, basal cytosolic [Ca.sup.2+] concentration ([[Ca.sup.2+].sub.i]) was also higher than in cells arrested in early G 1. Blocking hIK1 channels with a specific blocker, clotrimazole, induced both membrane potential depolarization and a decrease in the [[Ca.sup.2+].sub.i] in cells arrested at the end of G1 and S phases but not in cells arrested early in the G1 phase. Blocking hIK1 with clotrimazole also induced cell proliferation inhibition but to a lesser degree than blocking hEAG with astemizole. The two drugs were essentially additive, inhibiting MCF-7 cell proliferation by 82% and arresting >90% of cells in the GI phase. Thus, although the progression of MCF-7 cells through the early GI phase is dependent on the activation of hEAG [K.sup.+] channels, when it comes to G1 and checkpoint G1/S transition, the membrane potential appears to be primarily dependent on the hIK1-activity level. breast cancer; calcium-activated potassium channels; proliferation

Published
2004

23. Evolution of the human cold/menthol receptor, TRPM8

Author

Blanquart, Samuel, primary, Borowiec, Anne-sophie, additional, Delcourt, Philippe, additional, Figeac, Martin, additional, Emerling, Christopher A., additional, Meseguer, Andrea S., additional, Roudbaraki, Morad, additional, Prevarskaya, Natalia, additional, and Bidaux, Gabriel, additional

Published
2019
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26. Activation of mutated TRPA1 ion channel by resveratrol in human prostate cancer associated fibroblasts (CAF)

Author

Vancauwenberghe, Eric, primary, Noyer, Lucile, additional, Derouiche, Sandra, additional, Lemonnier, Loïc, additional, Gosset, Pierre, additional, Sadofsky, Laura R., additional, Mariot, Pascal, additional, Warnier, Marine, additional, Bokhobza, Alexandre, additional, Slomianny, Christian, additional, Mauroy, Brigitte, additional, Bonnal, Jean‐Louis, additional, Dewailly, Etienne, additional, Delcourt, Philippe, additional, Allart, Laurent, additional, Desruelles, Emilie, additional, Prevarskaya, Natalia, additional, and Roudbaraki, Morad, additional

Published
2017
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27. Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells

Author

Derouiche, Sandra, primary, Mariot, Pascal, additional, Warnier, Marine, additional, Vancauwenberghe, Eric, additional, Bidaux, Gabriel, additional, Gosset, Pierre, additional, Mauroy, Brigitte, additional, Bonnal, Jean-Louis, additional, Slomianny, Christian, additional, Delcourt, Philippe, additional, Dewailly, Etienne, additional, Prevarskaya, Natalia, additional, and Roudbaraki, Morad, additional

Published
2017
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29. Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation

Author

Borowiec, Anne‐Sophie, primary, Sion, Benoit, additional, Chalmel, Fréric, additional, Rolland, Antoine D., additional, Lemonnier, Loïc, additional, De Clerck, Tatiana, additional, Bokhobza, Alexandre, additional, Derouiche, Sandra, additional, Dewailly, Etienne, additional, Slomianny, Christian, additional, Mauduit, Claire, additional, Benahmed, Mohamed, additional, Roudbaraki, Morad, additional, Jégou, Bernard, additional, Prevarskaya, Natalia, additional, and Bidaux, Gabriel, additional

Published
2016
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30. Targeting of short TRPM8 isoforms induces 4TM-TRPM8-dependent apoptosis in prostate cancer cells

Author

Bidaux, Gabriel, primary, Borowiec, Anne-Sophie, additional, Dubois, Charlotte, additional, Delcourt, Philippe, additional, Schulz, Céline, additional, Abeele, Fabien Vanden, additional, Lepage, Gilbert, additional, Desruelles, Emilie, additional, Bokhobza, Alexandre, additional, Dewailly, Etienne, additional, Slomianny, Christian, additional, Roudbaraki, Morad, additional, Héliot, Laurent, additional, Bonnal, Jean-Louis, additional, Mauroy, Brigitte, additional, Mariot, Pascal, additional, Lemonnier, Loïc, additional, and Prevarskaya, Natalia, additional

Published
2016
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32. Orai1 contributes to the establishment of an apoptosis-resistant phenotype in prostate cancer cells

Author

Flourakis, Matthieu, Lehen'Kyi, V'Yacheslav, Beck, Benjamin, Raphael, Maylis, Vandenberghe, Matthieu, van Denabeele, Fabien, Roudbaraki, Morad, Lepage, Gilbert, Mauroy, Brigitte, Romanin, Christophe, Shuba, Yaroslave, Skryma, Roman, Prevarskaya, Natalia, Flourakis, Matthieu, Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe Hospitalier de l'Institut Catholique de Lille (GHICL), Université catholique de Lille (UCL), Institute for Biophysic, Johannes Kepler University Linz [Linz] (JKU), Bogomoletz Institute of Physiology, and This work was supported by grants from the Institut National de la Santé et de la Recherche Médicale (INSERM), Ministère de l'Education Nationale et Ligue Nationale Contre le Cancer.

Subjects
apoptosis resistance, [SDV.CAN] Life Sciences [q-bio]/Cancer, store-operated calcium entry, [SDV.CAN]Life Sciences [q-bio]/Cancer, prostate cancer, Orai channels
Abstract

International audience; The molecular nature of calcium (Ca(2+))-dependent mechanisms and the ion channels having a major role in the apoptosis of cancer cells remain a subject of debate. Here, we show that the recently identified Orai1 protein represents the major molecular component of endogenous store-operated Ca(2+) entry (SOCE) in human prostate cancer (PCa) cells, and constitutes the principal source of Ca(2+) influx used by the cell to trigger apoptosis. The downregulation of Orai1, and consequently SOCE, protects the cells from diverse apoptosis-inducing pathways, such as those induced by thapsigargin (Tg), tumor necrosis factor α, and cisplatin/oxaliplatin. The transfection of functional Orai1 mutants, such as R91W, a selectivity mutant, and L273S, a coiled-coil mutant, into the cells significantly decreased both SOCE and the rate of Tg-induced apoptosis. This suggests that the functional coupling of STIM1 to Orai1, as well as Orai1 Ca(2+)-selectivity as a channel, is required for its pro-apoptotic effects. We have also shown that the apoptosis resistance of androgen-independent PCa cells is associated with the downregulation of Orai1 expression as well as SOCE. Orai1 rescue, following Orai1 transfection of steroid-deprived cells, re-established the store-operated channel current and restored the normal rate of apoptosis. Thus, Orai1 has a pivotal role in the triggering of apoptosis, irrespective of apoptosis-inducing stimuli, and in the establishment of an apoptosis-resistant phenotype in PCa cells.

Published
2010

33. Differential role of transient receptor potential channels in Ca2+ entry and proliferation of prostate cancer epithelial cells.: Role of TRP Channels in Proliferation

Author

Thebault, Stephanie, Flourakis, Matthieu, Vanoverberghe, Karine, Vandermoere, Franck, Roudbaraki, Morad, Lehen'Kyi, V'Yacheslav, Slomianny, Christian, Beck, Benjamin, Mariot, Pascal, Bonnal, Jean-Louis, Mauroy, Brigitte, Shuba, Yaroslav, Capiod, Thierry, Skryma, Roman, Prevarskaya, Natalia, Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Signalisation des Facteurs de Croissance Dans Le Cancer du Sein . Proteomique Fonctionnelle, Bogomoletz Institute of Physiology NASU, and Flourakis, Matthieu

Subjects
MESH: Phenylephrine, MESH: Humans, MESH: NF-kappa B, MESH: NFATC Transcription Factors, MESH: Receptors, P, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Calcium Signaling, MESH: Male, [SDV.CAN] Life Sciences [q-bio]/Cancer, MESH: Epithelial Cells, MESH: Prostatic Neoplasms, MESH: Calcium, MESH: Adenosine Triphosphate, MESH: Cell Growth Processes, MESH: Adrenergic alpha-Agonists, MESH: Receptors, Adrenergic, alpha-1
Abstract

One major clinical problem with prostate cancer is the cells' ability to survive and proliferate upon androgen withdrawal. Because Ca2+ is central to growth control, understanding the mechanisms of Ca2+ homeostasis involved in prostate cancer cell proliferation is imperative for new therapeutic strategies. Here, we show that agonist-mediated stimulation of alpha1-adrenergic receptors (alpha1-AR) promotes proliferation of the primary human prostate cancer epithelial (hPCE) cells by inducing store-independent Ca2+ entry and subsequent activation of nuclear factor of activated T cells (NFAT) transcription factor. Such an agonist-induced Ca2+ entry (ACE) relied mostly on transient receptor potential canonical 6 (TRPC6) channels, whose silencing by antisense hybrid depletion decreased both hPCE cell proliferation and ACE. In contrast, ACE and related growth arrest associated with purinergic receptors (P2Y-R) stimulation involved neither TRPC6 nor NFAT. Our findings show that alpha1-AR signaling requires the coupled activation of TRPC6 channels and NFAT to promote proliferation of hPCE cells and thereby suggest TRPC6 as a novel potential therapeutic target.

Published
2006

34. Evidence for specific TRPM8 expression in human prostate secretory epithelial cells: functional androgen receptor requirement

Author

Bidaux, Gabriel, Roudbaraki, Morad, Merle, Corinne, Crépin, Alexandre, Delcourt, Philippe, Slomianny, Christian, Thébault, Stéphanie, Bonnal, Jean-Louis, Benahmed, Mohamed, Cabon, Florence, Mauroy, Brigitte, Prevarskaya, Natalia, Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la psysiopathologie de la prostate, Institut National de la Santé et de la Recherche Médicale (INSERM), Oncogénèse, différenciation et transduction du signal (ODTS), IFR89-Centre National de la Recherche Scientifique (CNRS), Communication Cellulaire en Biologie de la Reproduction, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Flourakis, Matthieu

Subjects
MESH: Neoplasm Proteins, MESH: 5-alpha-Dihydroprogesterone, MESH: Recepto, MESH: Humans, MESH: Myocytes, Smooth Muscle, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Gene Expression Regulation, Neoplastic, MESH: Prostate, MESH: Male, MESH: Promoter Regions (Genetics), [SDV.CAN] Life Sciences [q-bio]/Cancer, MESH: Epithelial Cells, MESH: Prostatic Neoplasms, MESH: Ion Channels, MESH: Androgens, MESH: RNA, Messenger
Abstract

TRPM8 (melastatine-related transient receptor potential member 8), a member of the transient receptor potential (TRP) superfamily of cation channels, has been shown to be a calcium-channel protein. TRPM8 mRNA has also been shown to be overexpressed in prostate cancer and is considered to play an important role in prostate physiology. This study was designed to determine the androgen-regulation mechanisms for TRPM8 mRNA expression and to identify the phenotype of TRPM8-expressing cells in the human prostate. Our findings show that trpm8 gene expression requires a functional androgen receptor. Furthermore, this article argues strongly in favour of the fact that the trpm8 gene is a primary androgen-responsive gene. Single-cell reverse transcriptase PCR and immunohistochemical experiments also showed that the trpm8 gene was mainly expressed in the apical secretory epithelial cells of the human prostate and trpm8 down-regulation occurred during the loss of the apical differentiated phenotype of the primary cultured human prostate epithelial cells. The androgen-regulated trpm8 expression mechanisms are important in understanding the progression of prostate cancer to androgen-independence. These findings may contribute to design a strategy to predict prostate cancer status from the TRPM8 mRNA level. Furthermore, as the TRPM8 channel is localized in human prostate cells, it will be interesting to understand its physiological function in the normal prostate and its potential role in prostate cancer development.

Published
2005

35. Regulation of activity of transient receptor potential melastatin 8 (TRPM8) channel by its short isoforms.

Author

Bidaux, Gabriel, Beck, Benjamin, Zholos, Alexander, Gordienko, Dmitri, Lemonnier, Loic, Flourakis, Matthieu, Roudbaraki, Morad, Borowiec, Anne-Sophie, Fernandez, Juan Pablo, Delcourt, Philippe, Lepage, G, Shuba, Yaroslav, Skryma, Roman, Prevarskaya, Natalia, Bidaux, Gabriel, Beck, Benjamin, Zholos, Alexander, Gordienko, Dmitri, Lemonnier, Loic, Flourakis, Matthieu, Roudbaraki, Morad, Borowiec, Anne-Sophie, Fernandez, Juan Pablo, Delcourt, Philippe, Lepage, G, Shuba, Yaroslav, Skryma, Roman, and Prevarskaya, Natalia

Abstract

One important mechanism of the regulation of membrane ion channels involves their nonfunctional isoforms generated by alternative splicing. However, knowledge of such isoforms for the members of the transient receptor potential (TRP) superfamily of ion channels remains quite limited. This study focuses on the TRPM8, which functions as a cold receptor in sensory neurons but is also expressed in tissues not exposed to ambient temperatures, as well as in cancer tissues. We report the cloning from prostate cancer cells of new short splice variants of TRPM8, termed short TRPM8α and short TRPM8β. Our results show that both variants are in a closed configuration with the C-terminal tail of the full-length TRPM8 channel, resulting in stabilization of its closed state and thus reducing both its cold sensitivity and activity. Our findings therefore uncover a new mode of regulation of the TRPM8 channel by its splice variants., Journal Article, Research Support, Non-U.S. Gov't, info:eu-repo/semantics/published

Published
2012

36. TRPC channels determine human keratinocyte differentiation: new insight into basal cell carcinoma.

Author

Beck, Benjamin, Lehen'kyi, V'yacheslav, Roudbaraki, Morad, Flourakis, Matthieu, Charveron, Marie, Bordat, Pascal, Polakowska, Renata, Prevarskaya, Natalia, Skryma, Roman, Beck, Benjamin, Lehen'kyi, V'yacheslav, Roudbaraki, Morad, Flourakis, Matthieu, Charveron, Marie, Bordat, Pascal, Polakowska, Renata, Prevarskaya, Natalia, and Skryma, Roman

Abstract

Aberrant keratinocyte differentiation is considered to be a key mechanism in the onset of hyperproliferative dermatological diseases, including basal cell carcinoma (BCC). It is, therefore, vital to understand what drives keratinocytes to develop such pathological phenotypes. The role of calcium in keratinocyte differentiation is uncontested but the mechanisms controlling calcium-induced differentiation have yet to be completely elucidated. This study was designed to investigate the role of calcium-permeable TRPC channels in human keratinocyte differentiation and BCC, using a combination of molecular and cell biology approaches, involving electrophysiology and Ca(2+)-imaging, on the HaCaT cell line, primary cultures of normal human keratinocytes, and BCC cells. We demonstrated that TRPC1/TRPC4 channel expression was important for keratinocyte differentiation, as knocking out these channels (by siRNA strategy) prevented the induction of Ca(2+)-induced differentiation. TRPC1/TRPC4-mediated calcium entry and endoplasmic reticulum Ca(2+) content increased significantly in differentiated keratinocytes. However, the failure of BCC cells to differentiate was related to a lack of TRPC channel expression and calcium entry. In summary, our data demonstrate that TRPC1 and TRPC4 channels are key elements in keratinocyte Ca(2+) homeostasis and differentiation and may therefore be responsible for skin pathologies., Journal Article, info:eu-repo/semantics/published

Published
2008

37. Functional coupling between large-conductance potassium channels and Cav3.2 voltage-dependent calcium channels participates in prostate cancer cell growth

Author

Gackière, Florian, primary, Warnier, Marine, additional, Katsogiannou, Maria, additional, Derouiche, Sandra, additional, Delcourt, Philippe, additional, Dewailly, Etienne, additional, Slomianny, Christian, additional, Humez, Sandrine, additional, Prevarskaya, Natalia, additional, Roudbaraki, Morad, additional, and Mariot, Pascal, additional

Published
2013
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38. Modulation of ER stress and apoptosis by endoplasmic reticulum calcium leakviatranslocon during unfolded protein response: involvement of GRP78

Author

Hammadi, Mehdi, primary, Oulidi, Agathe, additional, Gackière, Florian, additional, Katsogiannou, Maria, additional, Slomianny, Christian, additional, Roudbaraki, Morad, additional, Dewailly, Etienne, additional, Delcourt, Philippe, additional, Lepage, Gilbert, additional, Lotteau, Sabine, additional, Ducreux, Sylvie, additional, Prevarskaya, Natalia, additional, and Van Coppenolle, Fabien, additional

Published
2013
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39. Differential role of transient receptor potential channels in Ca2+ entry and proliferation of prostate cancer epithelial cells.

Author

Thebault, Stéphanie, Flourakis, Matthieu, Vanoverberghe, Karine, Vandermoere, Franck, Roudbaraki, Morad, Lehen'kyi, V'yacheslav, Slomianny, Christian, Beck, Benjamin, Mariot, Pascal, Bonnal, Jean-Louis, Mauroy, Brigitte, Shuba, Yaroslav, Capiod, Thierry, Skryma, Roman, Prevarskaya, Natalia, Thebault, Stéphanie, Flourakis, Matthieu, Vanoverberghe, Karine, Vandermoere, Franck, Roudbaraki, Morad, Lehen'kyi, V'yacheslav, Slomianny, Christian, Beck, Benjamin, Mariot, Pascal, Bonnal, Jean-Louis, Mauroy, Brigitte, Shuba, Yaroslav, Capiod, Thierry, Skryma, Roman, and Prevarskaya, Natalia

Abstract

One major clinical problem with prostate cancer is the cells' ability to survive and proliferate upon androgen withdrawal. Because Ca2+ is central to growth control, understanding the mechanisms of Ca2+ homeostasis involved in prostate cancer cell proliferation is imperative for new therapeutic strategies. Here, we show that agonist-mediated stimulation of alpha1-adrenergic receptors (alpha1-AR) promotes proliferation of the primary human prostate cancer epithelial (hPCE) cells by inducing store-independent Ca2+ entry and subsequent activation of nuclear factor of activated T cells (NFAT) transcription factor. Such an agonist-induced Ca2+ entry (ACE) relied mostly on transient receptor potential canonical 6 (TRPC6) channels, whose silencing by antisense hybrid depletion decreased both hPCE cell proliferation and ACE. In contrast, ACE and related growth arrest associated with purinergic receptors (P2Y-R) stimulation involved neither TRPC6 nor NFAT. Our findings show that alpha1-AR signaling requires the coupled activation of TRPC6 channels and NFAT to promote proliferation of hPCE cells and thereby suggest TRPC6 as a novel potential therapeutic target., Journal Article, Research Support, Non-U.S. Gov't, info:eu-repo/semantics/published

Published
2006

40. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms

Author

Bidaux, Gabriel, primary, Beck, Benjamin, additional, Zholos, Alexander, additional, Gordienko, Dmitri, additional, Lemonnier, Loic, additional, Flourakis, Matthieu, additional, Roudbaraki, Morad, additional, Borowiec, Anne-Sophie, additional, Fernández, José, additional, Delcourt, Philippe, additional, Lepage, Gilbert, additional, Shuba, Yaroslav, additional, Skryma, Roman, additional, and Prevarskaya, Natalia, additional

Published
2012
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41. Role of Cationic Channel TRPV2 in Promoting Prostate Cancer Migration and Progression to Androgen Resistance

Author

Monet, Michaël, primary, Lehen'kyi, V'yacheslav, additional, Gackiere, Florian, additional, Firlej, Virginie, additional, Vandenberghe, Matthieu, additional, Roudbaraki, Morad, additional, Gkika, Dimitra, additional, Pourtier, Albin, additional, Bidaux, Gabriel, additional, Slomianny, Christian, additional, Delcourt, Philippe, additional, Rassendren, François, additional, Bergerat, Jean-Pierre, additional, Ceraline, Jocelyn, additional, Cabon, Florence, additional, Humez, Sandrine, additional, and Prevarskaya, Natalia, additional

Published
2010
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44. Novel Role of Cold/Menthol-sensitive Transient Receptor Potential Melastatine Family Member 8 (TRPM8) in the Activation of Store-operated Channels in LNCaP Human Prostate Cancer Epithelial Cells

Author

Thebault, Stéphanie, primary, Lemonnier, Loïc, additional, Bidaux, Gabriel, additional, Flourakis, Matthieu, additional, Bavencoffe, Alexis, additional, Gordienko, Dimitri, additional, Roudbaraki, Morad, additional, Delcourt, Philippe, additional, Panchin, Yuri, additional, Shuba, Yaroslav, additional, Skryma, Roman, additional, and Prevarskaya, Natalia, additional

Published
2005
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48. Prolactin stimulates cell proliferation through a long form of prolactin receptor and K+ channel activation

Author

VAN COPPENOLLE, Fabien, primary, SKRYMA, Roman, additional, OUADID-AHIDOUCH, Halima, additional, SLOMIANNY, Christian, additional, ROUDBARAKI, Morad, additional, DELCOURT, Philippe, additional, DEWAILLY, Etienne, additional, HUMEZ, Sandrine, additional, CRÉPIN, Alexandre, additional, GOURDOU, Isabelle, additional, DJIANE, Jean, additional, BONNAL, Jean-Louis, additional, MAUROY, Brigitte, additional, and PREVARSKAYA, Natalia, additional

Published
2004
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