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Introduction. In vitro culture allows the genetic improvement of rose (Rosa hybrida), however, the acclimatization of plantlets in solid sustrate is a difficult process, it leads to malfuntion of stomata in the plants and consequently low carbon dioxide assimilation. Objective. The objective of this research was to evaluate three in vitro rooting media and different substrates for ex vitro acclimatization through solid and liquid media of transgenic rose. Materials and methods. transgenic rose (Rosa hybrida L.) cv. Classy plants were obtained from somatic embryo thatwere cultured in vitro for two years and were subjected rooting in vitro and ex vitro followed by acclimation. For in vitro rooting three different medium and for the ex vitro experiments, seven different combination of substrate were used. The study was carried out between August 2012 to January 2013 in Centro de Investigación y Asistencia de Tecnología y Diseño del Estado Jalisco, A.C. Results. The results from the in vitro and ex vitro experiments showed that the best in vitro rooting medium was the MS medium with the addition of 30 g/l sucrose and 0.5 g/l activated charcoal. The substrate composed of 70% peat moss and 30% pearlite was found to be the best for ex vitro conditions obtaining 20% of rooting. The substrate with 25% humus produced the highest chlorophyll content, however, there was no rooting. Solid treatments produced a higher percentage of seedling survival, high average number of leaves per plant, high chlorophyll content and a better sprout quality than hydroponics. However, the survival rate was 40% in the absence of aeration and 0% with aeration after sixteen days of the experiment. Treatment without aeration did not show any rooting. Higher contents of organic matter in the rooting substrate and the presence of aeration in a hydroponic solution did not guarantee a greater survival during acclimatization. Conclusion. Higher contents of organic matter in the rooting substrate and the presence of aeration in a hydroponic solution may not guarantee a greater survival during acclimatization. The results represent an optimization in the acclimatization of plants and the establishment of hydroponic culture of rose. Introducción. El cultivo in vitro permite el mejoramiento de Rosa hybrida, no obstante, la sobrevivencia de plántulas es difícil durante la aclimatación en sustrato sólido, el proceso conlleva a que las plantas presenten malfuncionamiento de los estomas y por ende, baja asimilación del dióxido de carbono. Objetivo. El objetivo de esta investigación fue evaluar tres medios de enraizamiento in vitro y diferentes sustratos para la aclimatación ex vitro mediante medios sólidos y líquidos de la rosa transgénica. Materiales y métodos. Se utilizaron plantas de rosa (Rosa hybrida L.) cv. Classy transgénica, a partir de embriones somáticos, cultivadas in vitro durante dos años y se sometieron a enraizamiento in vitro y ex vitro, seguido de aclimatación. Para enraizar in vitro tres medios diferentes y para los experimentos ex vitro, se utilizaron siete combinaciones diferentes de sustratos. El estudio se llevó a cabo de agosto de 2012 a enero de 2013 en el Centro de Investigación y Asistencia de Tecnología y Diseño del Estado de Jalisco, A.C. Resultados. Los resultados de los experimentos in vitro y ex vitro demostraron que los mejores resultados se obtuvieron en el medio MS adicionado con sacarosa (30 g/l) y carbón activado (0,5 g/l), y el sustrato compuesto de turba de musgo (70%) y perlita (30%), con 20% de enraizamiento. El sustrato con 25% de humus produjo el mayor contenido de clorofila, sin embargo, no hubo enraizamiento. Los tratamientos con sustrato sólido mostraron mayor porcentaje de sobrevivencia de plántulas, alto promedio de hojas por planta, alto contenido de clorofila y buena calidad del brote en comparación con los hidropónicos. La sobrevivencia en estos últimos fue de 40% sin aireación y 0% con aireación a los dieciséis días de establecimiento; ambos tratamientos no presentaron enraizamiento. Tratamientos sin aireación no mostraron enraizamiento. Conclusión. Mayores contenidos de materia orgánica en sustrato y la presencia de aireación en una solución hidropónica pueden no garantizar una mayor sobrevivencia durante la aclimatación. Los resultados suponen una optimización en la aclimatación de las plantas y el cultivo hidropónico de rosa.

Jatropha curcas brings attention across the developing world for biodiesel production since it grows in tropical and subtropical climates with no other competing food uses. This crop is gaining popularity in Mexico for biofuel production. Currently, there are not many reports on the native varieties and their yield to estimate the feasibility of the particular genetic resource to use it as an economic crop. So in this part of work an agronomical evaluation of yield, oil content, fatty acid types and biodiesel conversion from the two native varieties of Jatropha curcas from Mexico were compared while being produced in the same agro-climatic condition. The varieties used were based on the phorbol ester content like toxic and nontoxic varieties collected from Yucatan and Veracruz. It was found that the oil content in the seeds of toxic variety was 57% and oil content of the nontoxic variety was 54% and also the seed yield is higher. The fatty acid content also varies among the two varieties like 21% and 23% in toxic and nontoxic varieties respectively. Again in the nontoxic variety the content of monosaturated fatty acid was higher, which suggests that better quality biodiesel may be expected from this genotype. Transestrification of the two kinds of oil was done using KOH as a catalyst, but no significant difference was observed in the conversion of biodiesel among the two types of oil from the two genotypes.