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In the present study an attempt has been made to investigate the combined effect of sub-lethal dose of Cypermethrin 25% EC and pH on histoarchitecture of gonads and GnRH hormone of Puntius javanicus. 96h LC50 value was determined by method of Karber and it was found to be 3.65 µg/L for Puntius javanicus. Adult fish were exposed to sub-lethal concentration (0.365 µg/L) of Cypermethrin 25% EC in different pH of water, i.e. pH 5, pH 6, pH 7, pH 8.5 and pH 9.5 for 15 days. Significant histopathological alterations were found in testis and ovary of the fish subjected to sub-lethal concentration of cypermethrin 25% EC. Significant decrease (p?0.001) of GnRH concentration was recorded in pesticide exposed fishes. Significantly more histopathological alterations of gonads and decrease of GnRH concentration were recorded when fish were exposed to sub-lethal concentration of cypermethrin 25% EC in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin 25% EC in pH 7 water. A significant increase of GnRH concentration (p?0.05) was documented in fishes exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fishes exposed to sub-lethal concentration of cypermethrin in pH 7 and the concentration is found to be almost equal to the control group (pH 7, without toxicant). At this medium histoarchitecture of testis and ovary retained almost normal histology with least alterations.

In present study an attempt has been made to investigate the combined impact of sub lethal toxicity of Cypermethrin 25% EC and pH on certain blood parameters of Puntius javanicus. Adult fish were exposed to sublethal concentration (0.36 µg/L) of cypermethrin 25% EC in different pH water i.e. pH 5, pH 6, pH 7, pH 8.5 and pH 9.5 for 15 days. Significant changes in red blood cell (RBCs) count, total white blood cells (WBCs), haemoglobin % (Hb%), haematocrit value, total serum protein and blood glucose level were observed in fish exposed to sub-lethal concentration of cypermethrin. Significant decrease in red blood cell (RBCs) count, haemoglobin % (Hb %), haematocrit value and total serum protein content were found in fish exposed to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. A significant increase in these parameters were documented in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. On the other hand a significant increase of total white blood cells (WBCs) and blood glucose level were recorded in fish subjected to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. Values of these parameters were decreased significantly in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. The values of all studied blood parameters in fish subjected to sub-lethal concentration of cypermethrin in pH 8.5 were almost found to retain the values of control group (pH 7, without toxicant).