Search

Increasing evidence has linked ambient fine particulate matter (ie, particulate matter no larger than 2.5 μm [PMProspective cohort study.72,425 participants (age ≥18 years) without CKD were recruited from 121 counties in Hunan Province, China.Annual mean PMIncident CKD during the interval between the baseline examination of each participant (2005-2017) and the end of follow-up through 2018.Cox proportional hazards models were used to estimate the independent association of PMOver a median follow-up of 3.79 (IQR, 2.03-5.48) years, a total of 2,188 participants with incident CKD were identified. PMLack of information on participants' activity patterns such as time spent outdoors.Greater long-term ambient PMExposure to a form of air pollution known as fine particulate matter (ie, particulate matter ≤2.5 μm [PM

The β1‑adrenergic receptor (AR) is the primary β‑AR subtype in the heart and is the target of metoprolol (Met), which is commonly used to treat angina and hypertension. Previous studies have revealed a positive correlation between the methylation levels of the adrenoreceptor β1 gene (Adrb1) promoter in the myocardium with the antihypertensive activity of Met in spontaneously hypertensive rats (SHR), which affects β1‑AR expression in H9C2 cells. The aim of the present study was to investigate the effects of myocardial β1‑AR downregulation using short‑hairpin RNA (shRNA) against Adrb1 on the antihypertensive activity of Met in SHR. Recombinant adeno‑associated virus type 9 (rAAV9) vectors carrying Adrb1 shRNA (rAAV9‑Adrb1) or a negative control sequence (rAAV9‑NC) were generated and used to infect rat hearts via the pericardial cavity. The results of reverse transcription‑quantitative polymerase chain reaction, immunohistochemistry and western blotting analyses demonstrated that cardiac β1‑AR expression in the rAAV9‑Adrb1 group was significantly downregulated when compared with the rAAV9‑NC group (P