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Exosomes and microvesicles bear great potential to broaden therapeutic options in the clinical context. They differ in genesis, size, cargo, and composition despite their similarities. They were identified as participating in various processes such as angiogenesis, cell migration, and intracellular communication. Additionally, they are characterized by their natural biocompatibility. Therefore, researchers concluded that they could serve as a novel curative method capable of achieving unprecedented results. Indeed, in experiments, they proved remarkably efficient in enhancing wound regeneration and mitigating inflammation. Despite immense advancements in research on exosomes and microvesicles, the time for their large-scale application is yet to come. This article aims to gather and analyze current knowledge on those promising particles, their characteristics, and their potential clinical implementations. [ABSTRACT FROM AUTHOR]

It is impossible to imagine learning anatomy without properly fixating cadavers. Thanks to accurate preservation techniques, students can differentiate anatomical structures by their sight and touch. The formalin method was first described almost 150 years ago, and as such it may seem primitive, however it is effective and easy to use, which is why it is still in use today. Despite formaldehyde's bactericidal, fungicidal, and insecticidal properties contact with vapors of embalming solutions such as formalin may have a negative health effect. Skin drying, eczema, allergic contact dermatitis and lowered red blood cells (RBCs) and platelets are only a few symptoms that may occur as a result of spending long periods of time in places where cadavers are stored. Due to formalin's features, other techniques were invented. Thiel's method is also well known; after liquid application, tissues remain their natural color as the method is non-irritating and almost odorless. With the rise of technology, more modern methods of cadaver preservation were developed as alternative to formalin, such as the use of N-vinyl-2-pyrrolidone (NVP), which is commonly implemented in Japan and Brazil, and the Modified Larssen solution (MLS), which gives the possibility to reduce formalin concentration without losing the effectiveness of tissue preservation. The aim of this work is to develop an integrated approach to cadaver preservation through the analysis of frequently used preservation techniques. This is a considerable issue because first-year students of medicine and related faculties encounter the preserved material while learning anatomy. In comparison to more modern methods, formalin seems to be the worst choice due to worse joint flexibility, tissue colour and structure and, most importantly, its cancerogenic action. [ABSTRACT FROM AUTHOR]

Leukemia is a prevalent pediatric cancer with significant challenges, particularly in relapsed or refractory cases. Chimeric antigen receptor T-cell (CAR-T) therapy has emerged as a personalized cancer treatment, modifying patients' T cells to target and destroy resistant cancer cells. This study reviews the current therapeutic options of CAR-T therapy for leukemia, addressing the primary obstacles such as antigen escape and T-cell exhaustion. We explore dual-targeting strategies and their potential to improve treatment outcomes by preventing the loss of target antigens. Additionally, we examine the mechanisms of T-cell exhaustion and strategies to enhance CAR-T persistence and effectiveness. Despite remarkable clinical successes, CAR-T therapy poses risks such as cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Our findings highlight the need for ongoing research to optimize CAR-T applications, reduce toxicities, and extend this innovative therapy to a broader range of hematologic malignancies. This comprehensive review aims to provide valuable insights for improving leukemia treatment and advancing the field of cancer immunotherapy.