16 results on '"Ruffini, Francesca"'
Search Results
2. Rapamycin inhibits relapsing experimental autoimmune encephalomyelitis by both effector and regulatory T cells modulation
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Esposito, Marianna, Ruffini, Francesca, Bellone, Matteo, Gagliani, Nicola, Battaglia, Manuela, Martino, Gianvito, and Furlan, Roberto
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RAPAMYCIN , *CHEMICAL inhibitors , *TREATMENT of encephalomyelitis , *MULTIPLE sclerosis treatment , *T cells , *DRUG administration , *LABORATORY mice , *IMMUNOSUPPRESSION - Abstract
Abstract: Rapamycin is an oral immunosuppressant drug previously reported to efficiently induce naturally occurring CD4+CD25+FoxP3+ regulatory T (nTreg) cells re-establishing long-term immune self-tolerance in autoimmune diseases. We investigated the effect of rapamycin administration to SJL/j mice affected by PLP139–151-induced relapsing–remitting experimental autoimmune encephalomyelitis (RR-EAE). We found that oral or intraperitoneal treatment at the peak of disease or at the end of the first clinical attack, dramatically ameliorated the clinical course of RR-EAE. Treatment suspension resulted in early reappearance of disease. Clinical response was associated with reduced central nervous system demyelination and axonal loss. Rapamycin induced suppression of IFN-γ, and IL-17 release from antigen-specific T cells in peripheral lymphoid organs. While CD4+FoxP3+ cells were unaffected, we observed disappearance of CD4+CD45RBhigh effector T (Teff) cells and selective expansion of Treg cells bearing the CD4+CD45RBlowFoxP3+CD25+CD103+ extended phenotype. Finally, the dual action of rapamycin on both Teff and Treg cells resulted in modulation of their ratio that closely paralleled disease course. Our data show that rapamycin inhibits RR-EAE, provide evidence for the immunological mechanisms, and indicate this compound as a potential candidate for the treatment of multiple sclerosis. [Copyright &y& Elsevier]
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- 2010
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3. iAstrocytes do not restrain T cell proliferation in vitro.
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Colombo, Emanuela, De Angelis, Anthea, Bassani, Claudia, Ruffini, Francesca, Ottoboni, Linda, Garzetti, Livia, Finardi, Annamaria, Martino, Gianvito, Furlan, Roberto, and Farina, Cinthia
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T cells , *CELL proliferation , *T helper cells , *CELL physiology , *NERVE tissue - Abstract
The cross-talk between T cells and astrocytes occurring under physiological and, even more, neuroinflammatory conditions may profoundly impact the generation of adaptive immune responses in the nervous tissue. In this study, we used a standardized in vitro co-culture assay to investigate the immunomodulatory properties of astrocytes differing for age, sex, and species. Mouse neonatal astrocytes enhanced T cell vitality but suppressed T lymphocyte proliferation in response to mitogenic stimuli or myelin antigens, regardless of the Th1, Th2 or Th17 T cell phenotype. Studies comparing glia cells from adult and neonatal animals showed that adult astrocytes were more efficient in inhibiting T lymphocyte activation than neonatal astrocytes, regardless of their sex. Differently from primary cultures, mouse and human astrocytes derived from reprogrammed fibroblasts did not interfere with T cell proliferation. Overall, we describe a standardized astrocyte-T cell interaction in vitro assay and demonstrate that primary astrocytes and iAstrocytes may differ in modulating T cell function. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain.
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Luchetti, Alessandro, Milani, Davide, Ruffini, Francesca, Galli, Rossella, Falini, Andrea, Quattrini, Angelo, Scotti, Giuseppe, Comi, Giancarlo, Martino, Gianvito, Furlan, Roberto, and Politi, Letterio S.
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MONOCLONAL antibodies , *IRON oxides , *MAGNETIC resonance imaging , *LYMPHOCYTES , *IMMUNOHISTOCHEMISTRY , *LABORATORY mice , *CENTRAL nervous system - Abstract
We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T2 relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220+ cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3+ infiltrates in amurinemodel ofmultiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle. [ABSTRACT FROM AUTHOR]
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- 2012
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5. Extrinsic immune cell-derived, but not intrinsic oligodendroglial factors contribute to oligodendroglial differentiation block in multiple sclerosis.
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Starost, Laura, Lindner, Maren, Herold, Martin, Xu, Yu Kang T., Drexler, Hannes C. A., Heß, Katharina, Ehrlich, Marc, Ottoboni, Linda, Ruffini, Francesca, Stehling, Martin, Röpke, Albrecht, Thomas, Christian, Schöler, Hans R., Antel, Jack, Winkler, Jürgen, Martino, Gianvito, Klotz, Luisa, and Kuhlmann, Tanja
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OLIGODENDROGLIA , *MULTIPLE sclerosis , *MYELINATION , *DEMYELINATION , *PROGENITOR cells , *BLOOD cells - Abstract
Multiple sclerosis (MS) is the most frequent demyelinating disease in young adults and despite significant advances in immunotherapy, disease progression still cannot be prevented. Promotion of remyelination, an endogenous repair mechanism resulting in the formation of new myelin sheaths around demyelinated axons, represents a promising new treatment approach. However, remyelination frequently fails in MS lesions, which can in part be attributed to impaired differentiation of oligodendroglial progenitor cells into mature, myelinating oligodendrocytes. The reasons for impaired oligodendroglial differentiation and defective remyelination in MS are currently unknown. To determine whether intrinsic oligodendroglial factors contribute to impaired remyelination in relapsing–remitting MS (RRMS), we compared induced pluripotent stem cell-derived oligodendrocytes (hiOL) from RRMS patients and controls, among them two monozygous twin pairs discordant for MS. We found that hiOL from RRMS patients and controls were virtually indistinguishable with respect to remyelination-associated functions and proteomic composition. However, while analyzing the effect of extrinsic factors we discovered that supernatants of activated peripheral blood mononuclear cells (PBMCs) significantly inhibit oligodendroglial differentiation. In particular, we identified CD4+ T cells as mediators of impaired oligodendroglial differentiation; at least partly due to interferon-gamma secretion. Additionally, we observed that blocked oligodendroglial differentiation induced by PBMC supernatants could not be restored by application of oligodendroglial differentiation promoting drugs, whereas treatment of PBMCs with the immunomodulatory drug teriflunomide prior to supernatant collection partly rescued oligodendroglial differentiation. In summary, these data indicate that the oligodendroglial differentiation block is not due to intrinsic oligodendroglial factors but rather caused by the inflammatory environment in RRMS lesions which underlines the need for drug screening approaches taking the inflammatory environment into account. Combined, these findings may contribute to the development of new remyelination promoting strategies. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Convergence between Microglia and Peripheral Macrophages Phenotype during Development and Neuroinflammation.
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Grassivaro, Francesca, Menon, Ramesh, Acquaviva, Massimo, Ottoboni, Linda, Ruffini, Francesca, Bergamaschi, Andrea, Muzio, Luca, Farina, Cinthia, and Martino, Gianvito
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MACROPHAGES , *MICROGLIA , *INFLAMMATION , *BONE marrow , *YOLK sac - Abstract
Differently from other myeloid cells, microglia derive exclusively from precursors originating within the yolk sac and migrate to the CNS under development, without any contribution from fetal liver or postnatal hematopoiesis. Consistent with their unique ontology, microglia may express specific physiological markers, which have been partly described in recent years. Here we wondered whether profiles distinguishing microglia from peripheral macrophages vary with age and under pathology. To this goal, we profiled transcriptomes of microglia throughout the lifespan and included a parallel comparison with peripheral macrophages under physiological and neuroinflammatory settings using age- and sex-matched wild-type and bone marrow chimera mouse models. This comprehensive approach demonstrated that the phenotypic differentiation between microglia and peripheral macrophages is age-dependent and that peripheral macrophages do express some of the most commonly described microglia-specific markers early during development, such as Fcrls, P2ryl2, Tmeml 19, and Trem2. Further, during chronic neuroinflammation CNS-infiltrating macrophages and not peripheral myeloid cells acquire microglial markers, indicating that the CNS niche may instruct peripheral myeloid cells to gain the phenotype and, presumably, the function of the microglia cell. In conclusion, our data provide further evidence about the plasticity of the myeloid cell and suggest caution in the strict definition and application of microglia-specific markers. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Neural Stem Cells of the Sub ventricular Zone Contribute to Neuroprotection of the Corpus Callosum after Cuprizone-Induced Demyelination.
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Butti, Erica, Bacigaluppi, Marco, Chaabane, Linda, Ruffini, Francesca, Brambilla, Elena, Berera, Giulia, Montonati, Carolina, Quattrini, Angelo, and Martino, Gianvito
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NEURAL stem cells , *CORPUS callosum , *DEMYELINATION , *OLIGODENDROGLIA , *MULTIPLE sclerosis - Abstract
Myelin loss occurring in demyelinating diseases, including multiple sclerosis, is the leading cause of long-lasting neurological disability in adults. While endogenous remyelination, driven by resident oligodendrocyte precursor cells (OPCs), might partially compensate myelin loss in the early phases of demyelinating disorders, this spontaneous reparative potential fails at later stages. To investigate the cellular mechanisms sustaining endogenous remyelination in demyelinating disorders, we focused our attention on endogenous neural precursor cells (eNPCs) located within the subventricular zone (SVZ) since this latter area is considered one of the primary sources of new OPCs in the adult forebrain. First, we fate mapped SVZ-eNPCs in cuprizone-induced demyelination and found that SVZ endogenous neural stem/precursor cells are recruited during the remyelination phase to the corpus callosum (CC) and are capable of forming new oligodendrocytes. When we ablated SVZ-derived eNPCs during cuprizone-induced demyelination in female mice, the animals displayed reduced numbers of oligodendrocytes within the lesioned CC. Although this reduction in oligodendrocytes did not impact the ensuing remyelination, eNPC-ablated mice experienced increased axonal loss. Our results indicate that, in toxic models of demyelination, SVZ-derived eNPCs contribute to support axonal survival. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Role of endogenous neural precursor cells in demyelination and remyelination after cuprizone-induced injury.
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Butti, Erica, Berera, Giulia, Ruffini, Francesca, Chaabane, Linda, Dina, Giorgia, Quattrini, Angelo, Comi, Giancarlo, and Martino, Gianvito
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DEMYELINATION , *MYELIN , *OLIGODENDROGLIA , *MULTIPLE sclerosis , *TISSUE wounds - Published
- 2014
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9. IL4 induces IL6-producing M2 macrophages associated to inhibition of neuroinflammation in vitro and in vivo.
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Casella, Giacomo, Garzetti, Livia, Gatta, Alberto T., Finardi, Annamaria, Maiorino, Chiara, Ruffini, Francesca, Martino, Gianvito, Muzio, Luca, and Furlan, Roberto
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INTERLEUKIN-4 , *AUTOIMMUNE disease treatment , *THERAPEUTICS , *NEUROLOGICAL disorders , *ANIMAL models of inflammation , *ANTI-inflammatory agents , *INTERLEUKIN-6 , *MACROPHAGES , *INFLAMMATION prevention , *ANIMAL experimentation , *CELL culture , *DOSE-effect relationship in pharmacology , *INFLAMMATION , *INFLAMMATORY mediators , *INTERLEUKINS , *RESEARCH methodology , *MICE , *TISSUE culture - Abstract
Background: Myeloid cells, such as macrophages and microglia, play a crucial role in neuroinflammation and have been recently identified as a novel therapeutic target, especially for chronic forms. The general aim would be to change the phenotype of myeloid cells from pro- to anti-inflammatory, favoring their tissue-trophic and regenerative functions. Myeloid cells, however, display a number of functional phenotypes, not immediately identifiable as pro- or anti-inflammatory, and associated to ambiguous markers.Methods: We employed in vitro assays to study macrophage polarization/differentiation in the presence of classical polarizing stimuli such as IFNγ (pro-inflammatory) and IL4 (anti-inflammatory). We induced neuroinflammation in mice by immunization with a myelin antigen and treated diseased mice with intracisternal delivery of an IL4-expressing lentiviral vector. We analyzed clinical, pathological, and immunological outcomes with a focus on myeloid cells.Results: We found that IL6, usually considered a pro-inflammatory cytokine, was released in vitro by macrophages treated with the anti-inflammatory cytokine IL4. We show the existence of macrophages expressing IL6 along with classical anti-inflammatory markers such as CD206 and demonstrate that these cells are immunosuppressive in vitro. In neuroinflamed mice, we show that IL4 delivery in the central nervous system (CNS) is associated with clinical and pathological protection from disease, associated with increased IL6 expression in infiltrating macrophages.Conclusions: IL6 is known to mediate both pro- and anti-inflammatory effects, having two distinct ways to induce cell-signaling: either through the membrane bound receptor (anti-inflammatory) or through trans-signaling (pro-inflammatory). We show here that IL6-expressing macrophages are associated to protection from neuroinflammation, suggesting that IL6 anti-inflammatory properties prevail in the CNS, and calling for a general reconsideration of IL6 in macrophage polarization. [ABSTRACT FROM AUTHOR]- Published
- 2016
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10. Influence of experimental autoimmune encephalomyelitis on the localization and viability of neural stem cells after intrathecal transplantation.
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Merlini, Arianna, De Feo, Donatella, Ruffini, Francesca, Brambilla, Elena, Comi, Giancarlo, and Martino, Gianvito
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AUTOIMMUNE diseases , *ENCEPHALOMYELITIS , *CENTRAL nervous system diseases , *NEURAL stem cells , *CELL transplantation , *NEUROIMMUNOLOGY - Published
- 2014
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11. Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis.
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Sardi, Claudia, Zambusi, Laura, Finardi, Annamaria, Ruffini, Francesca, Tolun, Adviye A., Dickerson, Ian M., Righi, Marco, Zacchetti, Daniele, Grohovaz, Fabio, Provini, Luciano, Furlan, Roberto, and Morara, Stefano
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CALCITONIN gene-related peptide , *AUTOIMMUNE diseases , *ENCEPHALOMYELITIS , *MICROGLIA , *INFLAMMATION , *CEREBROSPINAL fluid - Abstract
Abstract: Calcitonin Gene-Related Peptide (CGRP) inhibits microglia inflammatory activation in vitro. We here analyzed the involvement of CGRP and Receptor Component Protein (RCP) in experimental autoimmune encephalomyelitis (EAE). Alpha-CGRP deficiency increased EAE scores which followed the scale alpha-CGRP null>heterozygote>wild type. In wild type mice, CGRP delivery into the cerebrospinal fluid (CSF) 1) reduced chronic EAE (C-EAE) signs, 2) inhibited microglia activation (revealed by quantitative shape analysis), and 3) did not alter GFAP expression, cell density, lymphocyte infiltration, and peripheral lymphocyte production of IFN‐gamma, TNF-alpha, IL-17, IL-2, and IL-4. RCP (probe for receptor involvement) was expressed in white matter microglia, astrocytes, oligodendrocytes, and vascular-endothelial cells: in EAE, also in infiltrating lymphocytes. In relapsing–remitting EAE (R-EAE) RCP increased during relapse, without correlation with lymphocyte density. RCP nuclear localization (stimulated by CGRP in vitro) was I) increased in microglia and decreased in astrocytes (R-EAE), and II) increased in microglia by CGRP CSF delivery (C-EAE). Calcitonin like receptor was rarely localized in nuclei of control and relapse mice. CGRP increased in motoneurons. In conclusion, CGRP can inhibit microglia activation in vivo in EAE. CGRP and its receptor may represent novel protective factors in EAE, apparently acting through the differential cell-specific intracellular translocation of RCP. [Copyright &y& Elsevier]
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- 2014
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12. Myeloid microvesicles are a marker and therapeutic target for neuroinflammation.
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Verderio, Claudia, Muzio, Luca, Turola, Elena, Bergami, Alessandra, Novellino, Luisa, Ruffini, Francesca, Riganti, Loredana, Corradini, Irene, Francolini, Maura, Garzetti, Livia, Maiorino, Chiara, Servida, Federica, Vercelli, Alessandro, Rocca, Mara, Libera, Dacia Dalla, Martinelli, Vittorio, Comi, Giancarlo, Martino, Gianvito, Matteoli, Michela, and Furlan, Roberto
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Objective: Microvesicles (MVs) have been indicated as important mediators of intercellular communication and are emerging as new biomarkers of tissue damage. Our previous data indicate that reactive microglia/macrophages release MVs in vitro. The aim of the study was to evaluate whether MVs are released by microglia/macrophages in vivo and whether their number varies in brain inflammatory conditions, such as multiple sclerosis (MS). Methods: Electron and fluorescence microscopy and flow cytometry were used to detect myeloid MVs in the cerebrospinal fluid (CSF) of healthy controls, MS patients, and rodents affected by experimental autoimmune encephalomyelitis (EAE), the animal model of MS. Results: Myeloid MVs were detected in CSF of healthy controls. In relapsing and remitting EAE mice, the concentration of myeloid MVs in the CSF was significantly increased and closely associated with disease course. Analysis of MVs in the CSF of 28 relapsing patients and 28 patients with clinical isolated syndrome from 2 independent cohorts revealed higher levels of myeloid MVs than in 13 age-matched controls, indicating a clinical value of MVs as a companion tool to capture disease activity. Myeloid MVs were found to spread inflammatory signals both in vitro and in vivo at the site of administration; mice impaired in MV shedding were protected from EAE, suggesting a pathogenic role for MVs in the disease. Finally, FTY720, the first approved oral MS drug, significantly reduced the amount of MVs in the CSF of EAE-treated mice. Interpretation: These findings identify myeloid MVs as a marker and therapeutic target of brain inflammation. ANN NEUROL 2012;72:610-624 [ABSTRACT FROM AUTHOR]
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- 2012
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13. 2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis
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Farinazzo, Alessia, Gini, Beatrice, Milli, Alberto, Ruffini, Francesca, Marconi, Silvia, Turano, Ermanna, Anghileri, Elena, Barbieri, Francesca, Cecconi, Daniela, Furlan, Roberto, and Bonetti, Bruno
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AUTOANTIBODIES , *IMMUNOGLOBULIN G , *MULTIPLE sclerosis , *ENZYME-linked immunosorbent assay , *POLYACRYLAMIDE gel electrophoresis , *MIMICRY (Chemistry) , *ENCEPHALOMYELITIS , *NERVE tissue proteins - Abstract
Abstract: 2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide89–104, total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis. [Copyright &y& Elsevier]
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- 2011
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14. Laquinimod Modulates Human Astrocyte Function and Dampens Astrocyte-Induced Neurotoxicity during Inflammation.
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Colombo, Emanuela, Pascente, Rosaria, Triolo, Daniela, Bassani, Claudia, De Angelis, Anthea, Ruffini, Francesca, Ottoboni, Linda, Comi, Giancarlo, Martino, Gianvito, Farina, Cinthia, and Filippis, Barbara De
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GLUTAMATE transporters , *ARYL hydrocarbon receptors , *NEUROTOXICOLOGY , *ASTROCYTES , *INFLAMMATORY mediators , *INFLAMMATION , *NEURODEGENERATION - Abstract
Astrocytes greatly participate to inflammatory and neurotoxic reactions occurring in neurodegenerative diseases and are valuable pharmacological targets to support neuroprotection. Here we used human astrocytes generated from reprogrammed fibroblasts as a cellular model to study the effect of the compound Laquinimod and its active metabolite de-Laquinimod on astrocyte functions and the astrocyte–neuron interaction. We show that human iAstrocytes expressed the receptor for the inflammatory mediator IL1 and responded to it via nuclear translocation of NFκB, an event that did not occur if cells were treated with Laquinimod, indicating a direct anti-inflammatory activity of the drug on the human astrocyte. Similarly, while exposure to IL1 downregulated glial glutamate transporters GLAST and GLT1, treatment with Laquinimod supported maintenance of physiological levels of these proteins despite the inflammatory milieu. Laquinimod also induced nuclear translocation of the aryl hydrocarbon receptor (AHR), suggesting that drug action was mediated by activation of the AHR pathway. However, the drug was effective despite AHR inhibition via CH223191, indicating that AHR signaling in the astrocyte is dispensable for drug responses. Finally, in vitro experiments with rat spinal neurons showed that laquinimod did not exert neuroprotection directly on the neuron but dampened astrocyte-induced neurodegeneration. Our findings indicate that fibroblast-derived human astrocytes represent a suitable model to study astrocyte–neuron crosstalk and demonstrate indirect, partial neuroprotective efficacy for laquinimod. [ABSTRACT FROM AUTHOR]
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- 2020
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15. Generation and characterization of iPSC-derived neurons from multiple sclerosis patients.
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Laterza, Cecilia, De Ceglia, Roberta, Pinzani, Lorenzo, Ruffini, Francesca, Zoia, Chiara Paola, Peitz, Michael, and Martino, Gianvito
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NEURON analysis , *MULTIPLE sclerosis treatment , *AUTOIMMUNE diseases , *DEMYELINATION , *CENTRAL nervous system diseases - Published
- 2014
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16. Intrathecal transplantation of neural precursor cells impairs the effector phase of experimental autoimmune encephalomyelitis.
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De Feo, Donatella, Merlini, Arianna, Brambilla, Elena, Laterza, Cecilia, Ruffini, Francesca, Menon, Ramesh, Farina, Cinthia, Comi, Giancarlo, Greter, Melanie, and Martino, Gianvito
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NEURAL receptors , *ENCEPHALOMYELITIS , *PROTEIN precursors , *NEUROIMMUNOLOGY ,SPINAL cord transplantation - Published
- 2014
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