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1. Genomic analysis of Plasmodium vivax describes patterns of connectivity and putative drivers of adaptation in Ethiopia

Author

Kebede, Alebachew Messele, Sutanto, Edwin, Trimarsanto, Hidayat, Benavente, Ernest Diez, Barnes, Mariana, Pearson, Richard D., Siegel, Sasha V., Erko, Berhanu, Assefa, Ashenafi, Getachew, Sisay, Aseffa, Abraham, Petros, Beyene, Lo, Eugenia, Mohammed, Rezika, Yilma, Daniel, Rumaseb, Angela, Nosten, Francois, Noviyanti, Rintis, Rayner, Julian C., Kwiatkowski, Dominic P., Price, Ric N., Golassa, Lemu, and Auburn, Sarah

Published
2023
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2. Genomics of Plasmodium vivax in Colombia reveals evidence of local bottle-necking and inter-country connectivity in the Americas

Author

Sutanto, Edwin, Pava, Zuleima, Echeverry, Diego F., Lopera-Mesa, Tatiana M., Montenegro, Lidia Madeline, Yasnot-Acosta, Maria F., Benavente, Ernest Diez, Pearson, Richard D., Herrera, Sócrates, Arévalo-Herrera, Myriam, Trimarsanto, Hidayat, Rumaseb, Angela, Noviyanti, Rintis, Kwiatkowski, Dominic P., Price, Ric N., and Auburn, Sarah

Published
2023
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3. Primaquine radical cure in patients with Plasmodium falciparum malaria in areas co-endemic for P falciparum and Plasmodium vivax (PRIMA): a multicentre, open-label, superiority randomised controlled trial

Author

Thriemer, Kamala, Degaga, Tamiru Shibiru, Christian, Michael, Alam, Mohammad Shafiul, Rajasekhar, Megha, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagamawie Tadesse, Weston, Sophie, Mnjala, Hellen, Rumaseb, Angela, Satyagraha, Ari Winasti, Sadhewa, Arkasha, Panggalo, Lydia Vista, Ekawati, Lenny L, Lee, Grant, Anose, Rodas Temesgen, Kiros, Fitsum Getahun, Simpson, Julie A, Karahalios, Amalia, Woyessa, Adugna, Baird, J Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N

Published
2023
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4. Reducing the risk of Plasmodium vivax after falciparum infections in co-endemic areas—a randomized controlled trial (PRIMA)

Author

Thriemer, Kamala, Degaga, Tamiru Shibru, Christian, Michael, Alam, Mohammad Shafiul, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagimawie Tadesse, Weston, Sophie, Karahalios, Amalia, Rajasekhar, Megha, Simpson, Julie A., Rumaseb, Angela, Mnjala, Hellen, Lee, Grant, Anose, Rodas Temesgen, Kidane, Fitsum Getahun, Woyessa, Adugna, Baird, Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N.

Published
2022
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6. Longitudinal ex vivo and molecular trends of chloroquine and piperaquine activity against Plasmodium falciparum and P. vivax before and after introduction of artemisinin-based combination therapy in Papua, Indonesia

Author

Marfurt, Jutta, Wirjanata, Grennady, Prayoga, Pak, Chalfein, Ferryanto, Leonardo, Leo, Sebayang, Boni F., Apriyanti, Dwi, Sihombing, Maic A.E.M., Trianty, Leily, Suwanarusk, Rossarin, Brockman, Alan, Piera, Kim A., Luo, Irene, Rumaseb, Angela, MacHunter, Barbara, Auburn, Sarah, Anstey, Nicholas M., Kenangalem, Enny, Noviyanti, Rintis, Russell, Bruce, Poespoprodjo, Jeanne R., and Price, Ric N.

Published
2021
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7. Field assessment of the operating procedures of a semi-quantitative G6PD Biosensor to improve repeatability of routine testing

Author

Sadhewa, Arkasha, primary, Chaudhary, Alina, additional, Panggalo, Lydia V., additional, Rumaseb, Angela, additional, Adhikari, Nabaraj, additional, Adhikari, Sanjib, additional, Rijal, Komal Raj, additional, Banjara, Megha Raj, additional, Price, Ric N., additional, Thriemer, Kamala, additional, Ghimire, Prakash, additional, Ley, Benedikt, additional, and Satyagraha, Ari Winasti, additional

Published
2024
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8. Genomic analysis of Plasmodium vivax identifies putative drivers of adaptation and connectivity across diverse districts in Ethiopia

Author

Kebede, Alebachew Messele, primary, Sutanto, Edwin, additional, Trimarsanto, Hidayat, additional, Benavente, Ernest Diez, additional, Barnes, Mariana, additional, Pearson, Richard, additional, Siegel, Sasha, additional, Erko, Berhanu, additional, Assefa, Ashenafi, additional, Getachew, Sisay, additional, Aseffa, Abraham, additional, Petros, Beyene, additional, Lo, Eugenia, additional, Mohammed, Rezika, additional, Yilma, Daniel, additional, Rumaseb, Angela, additional, Nosten, Francois, additional, Noviyanti, Rintis, additional, Rayner, Julian, additional, Kwiatkowski, Dominic, additional, Price, Ric, additional, Golassa, Lemu, additional, and Auburn, Sarah, additional

Published
2023
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9. Primaquine radical cure in patients with Plasmodium falciparummalaria in areas co-endemic for P falciparumand Plasmodium vivax(PRIMA): a multicentre, open-label, superiority randomised controlled trial

Author

Thriemer, Kamala, Degaga, Tamiru Shibiru, Christian, Michael, Alam, Mohammad Shafiul, Rajasekhar, Megha, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagamawie Tadesse, Weston, Sophie, Mnjala, Hellen, Rumaseb, Angela, Satyagraha, Ari Winasti, Sadhewa, Arkasha, Panggalo, Lydia Vista, Ekawati, Lenny L, Lee, Grant, Anose, Rodas Temesgen, Kiros, Fitsum Getahun, Simpson, Julie A, Karahalios, Amalia, Woyessa, Adugna, Baird, J Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N

Abstract

In areas co-endemic for Plasmodium vivaxand Plasmodium falciparumthere is an increased risk of P vivaxparasitaemia following P falciparummalaria. Radical cure is currently only recommended for patients presenting with P vivaxmalaria. Expanding the indication for radical cure to patients presenting with P falciparummalaria could reduce their risk of subsequent P vivaxparasitaemia.

Published
2023
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10. Adapting international clinical trials during COVID-19 and beyond

Author

Thriemer, Kamala, primary, Degaga, Tamiru Shibiru, additional, Alam, Mohammad Shafiul, additional, Adhikari, Bipin, additional, Tripura, Rupam, additional, Hossain, Mohammad Sharif, additional, Christian, Michael, additional, Ghanchi, Najia K, additional, Mnjala, Hellen, additional, Weston, Sophie, additional, Ley, Benedikt, additional, Rumaseb, Angela, additional, Tadesse, Dagimawie, additional, Teferi, Tedla, additional, Yilma, Daniel, additional, Lee, Grant, additional, Unger, Holger, additional, Sutanto, Inge, additional, Pasaribu, Ayodhia Pitaloka, additional, Ghimire, Prakash, additional, Beg, Mohammad Asim, additional, and Price, Ric N, additional

Published
2023
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11. Can non‐typeable Haemophilus influenzae carriage surveillance data infer antimicrobial resistance associated with otitis media?

Author

Bowman‐Derrick, Sophia, primary, Harris, Tegan M., additional, Beissbarth, Jemima, additional, Kleinecke, Mariana, additional, Lawrence, Katrina, additional, Wozniak, Teresa M., additional, Bleakley, Amy, additional, Rumaseb, Angela, additional, Binks, Michael J., additional, Marsh, Robyn L., additional, Morris, Peter S., additional, Leach, Amanda J., additional, and Smith‐Vaughan, Heidi, additional

Published
2023
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13. No association between thePlasmodium vivax crt-oMS334 or In9pvcrtpolymorphisms and chloroquine failure in a clinical cohort from Malaysia

Author

Rumaseb, Angela, primary, Moraes Barros, Roberto R., additional, Sá, Juliana M., additional, Juliano, Jonathan J., additional, William, Timothy, additional, Braima, Kamil A., additional, Barber, Bridget E., additional, Anstey, Nicholas M, additional, Price, Ric N., additional, Grigg, Matthew J., additional, Auburn, Sarah, additional, and Marfurt, Jutta, additional

Published
2022
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14. An open dataset of Plasmodium vivax genome variation in 1,895 worldwide samples

Author

Adam, Ishag, Alam, Mohammad Shafiul, Alemu, Sisay, Amaratunga, Chanaki, Amato, Roberto, Andrianaranjaka, Voahangy, Anstey, Nicholas M, Aseffa, Abraham, Ashley, Elizabeth, Assefa, Ashenafi, Auburn, Sarah, Barber, Bridget E, Barry, Alyssa, Batista Pereira, Dhelio, Cao, Jun, Chau, Nguyen Hoang, Chotivanich, Kesinee, Chu, Cindy, Dondorp, Arjen M, Drury, Eleanor, Echeverry, Diego F, Erko, Berhanu, Espino, Fe, Fairhurst, Rick, Faiz, Abdul, Fernanda Villegas, María, Gao, Qi, Golassa, Lemu, Goncalves, Sonia, Grigg, Matthew J, Hamedi, Yaghoob, Hien, Tran Tinh, Htut, Ye, Johnson, Kimberly J, Karunaweera, Nadira, Khan, Wasif, Krudsood, Srivicha, Kwiatkowski, Dominic P, Lacerda, Marcus, Ley, Benedikt, Lim, Pharath, Liu, Yaobao, Llanos-Cuentas, Alejandro, Lon, Chanthap, Lopera-Mesa, Tatiana, Marfurt, Jutta, Michon, Pascal, Miotto, Olivo, Mohammed, Rezika, Mueller, Ivo, Namaik-Larp, Chayadol, Newton, Paul N, Nguyen, Thuy-Nhien, Nosten, Francois, Noviyanti, Rintis, Pava, Zuleima, Pearson, Richard D, Petros, Beyene, Phyo, Aung P, Price, Ric N, Pukrittayakamee, Sasithon, Rahim, Awab Ghulam, Randrianarivelojosia, Milijaona, Rayner, Julian C, Rumaseb, Angela, Siegel, Sasha V, Simpson, Victoria J, Thriemer, Kamala, Tobon-Castano, Alberto, Trimarsanto, Hidayat, Urbano Ferreira, Marcelo, Vélez, Ivan D, Wangchuk, Sonam, Wellems, Thomas E, White, Nicholas J, William, Timothy, Yasnot, Maria F, Yilma, Daniel, Alam, Mohammad Shafiul [0000-0001-8330-5499], Ashley, Elizabeth [0000-0002-7620-4822], Barber, Bridget E [0000-0003-1066-7960], Batista Pereira, Dhelio [0000-0002-7761-5498], Chu, Cindy [0000-0001-9465-8214], Dondorp, Arjen M [0000-0001-5190-2395], Echeverry, Diego F [0000-0003-0301-4478], Espino, Fe [0000-0003-1690-1711], Faiz, Abdul [0000-0002-3460-7535], Golassa, Lemu [0000-0002-1216-8711], Grigg, Matthew J [0000-0001-9914-8352], Karunaweera, Nadira [0000-0003-3985-1817], Kwiatkowski, Dominic P [0000-0002-5023-0176], Ley, Benedikt [0000-0002-5734-0845], Miotto, Olivo [0000-0001-8060-6771], Nguyen, Thuy-Nhien [0000-0002-4101-5706], Nosten, Francois [0000-0002-7951-0745], Pearson, Richard D [0000-0002-7386-3566], Phyo, Aung P [0000-0002-0383-9624], Price, Ric N [0000-0003-2000-2874], Rayner, Julian C [0000-0002-9835-1014], Urbano Ferreira, Marcelo [0000-0002-5293-9090], Wellems, Thomas E [0000-0003-3899-8454], Yasnot, Maria F [0000-0001-8081-4212], Yilma, Daniel [0000-0001-6058-2696], and Apollo - University of Cambridge Repository

Subjects
parasitic diseases, Genomics, Genomic Epidemiology, Plasmodium vivax, Malaria, Data Resource
Abstract

This report describes the MalariaGEN Pv4 dataset, a new release of curated genome variation data on 1,895 samples of Plasmodium vivax collected at 88 worldwide locations between 2001 and 2017. It includes 1,370 new samples contributed by MalariaGEN and VivaxGEN partner studies in addition to previously published samples from these and other sources. We provide genotype calls at over 4.5 million variable positions including over 3 million single nucleotide polymorphisms (SNPs), as well as short indels and tandem duplications. This enlarged dataset highlights major compartments of parasite population structure, with clear differentiation between Africa, Latin America, Oceania, Western Asia and different parts of Southeast Asia. Each sample has been classified for drug resistance to sulfadoxine, pyrimethamine and mefloquine based on known markers at the dhfr, dhps and mdr1 loci. The prevalence of all of these resistance markers was much higher in Southeast Asia and Oceania than elsewhere. This open resource of analysis-ready genome variation data from the MalariaGEN and VivaxGEN networks is driven by our collective goal to advance research into the complex biology of P. vivax and to accelerate genomic surveillance for malaria control and elimination.

Published
2022

15. Additional file 1 of A fluorometric assay to determine the protective effect of glucose-6-phosphate dehydrogenase (G6PD) against a Plasmodium spp. infection in females heterozygous for the G6PD gene: proof of concept in Plasmodium falciparum

Author

Rumaseb, Angela, Marfurt, Jutta, Kho, Steven, Kahn, Maria, Price, Ric N., and Ley, Benedikt

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, hemic and lymphatic diseases, parasitic diseases, nutritional and metabolic diseases
Abstract

Additional file 1. Standard operating procedure for G6PD analysis in Plasmodium-infected red blood cells.

Published
2022
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16. Additional file 1 of Reducing the risk of Plasmodium vivax after falciparum infections in co-endemic areas—a randomized controlled trial (PRIMA)

Author

Thriemer, Kamala, Degaga, Tamiru Shibru, Christian, Michael, Alam, Mohammad Shafiul, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagimawie Tadesse, Weston, Sophie, Karahalios, Amalia, Rajasekhar, Megha, Simpson, Julie A., Rumaseb, Angela, Mnjala, Hellen, Lee, Grant, Anose, Rodas Temesgen, Kidane, Fitsum Getahun, Woyessa, Adugna, Baird, Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N.

Subjects
Data_FILES
Abstract

Additional file 1. Rationale for study.

Published
2022
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17. Additional file 2 of A fluorometric assay to determine the protective effect of glucose-6-phosphate dehydrogenase (G6PD) against a Plasmodium spp. infection in females heterozygous for the G6PD gene: proof of concept in Plasmodium falciparum

Author

Rumaseb, Angela, Marfurt, Jutta, Kho, Steven, Kahn, Maria, Price, Ric N., and Ley, Benedikt

Subjects
parasitic diseases
Abstract

Additional file 2. Flow cytometry data from schizont and ring stages of P. falciparum FC27.

Published
2022
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18. Additional file 3 of Reducing the risk of Plasmodium vivax after falciparum infections in co-endemic areas—a randomized controlled trial (PRIMA)

Author

Thriemer, Kamala, Degaga, Tamiru Shibru, Christian, Michael, Alam, Mohammad Shafiul, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagimawie Tadesse, Weston, Sophie, Karahalios, Amalia, Rajasekhar, Megha, Simpson, Julie A., Rumaseb, Angela, Mnjala, Hellen, Lee, Grant, Anose, Rodas Temesgen, Kidane, Fitsum Getahun, Woyessa, Adugna, Baird, Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N.

Subjects
Data_FILES
Abstract

Additional file 3. Statistical Analysis Plan.

Published
2022
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19. An open dataset of Plasmodium vivax genome variation in 1,895 worldwide samples

Author

MalariaGEN, Adam, Ishag, Alam, Mohammad Shafiul, Alemu, Sisay, Amaratunga, Chanaki, Amato, Roberto, Andrianaranjaka, Voahangy, Anstey, Nicholas M, Aseffa, Abraham, Ashley, Elizabeth, Assefa, Ashenafi, Auburn, Sarah, Barber, Bridget E, Barry, Alyssa, Batista Pereira, Dhelio, Cao, Jun, Chau, Nguyen Hoang, Chotivanich, Kesinee, Chu, Cindy, Dondorp, Arjen M, Drury, Eleanor, Echeverry, Diego F, Erko, Berhanu, Espino, Fe, Fairhurst, Rick, Faiz, Abdul, Fernanda Villegas, María, Gao, Qi, Golassa, Lemu, Goncalves, Sonia, Grigg, Matthew J, Hamedi, Yaghoob, Hien, Tran Tinh, Htut, Ye, Johnson, Kimberly J, Karunaweera, Nadira, Khan, Wasif, Krudsood, Srivicha, Kwiatkowski, Dominic P, Lacerda, Marcus, Ley, Benedikt, Lim, Pharath, Liu, Yaobao, Llanos-Cuentas, Alejandro, Lon, Chanthap, Lopera-Mesa, Tatiana, Marfurt, Jutta, Michon, Pascal, Miotto, Olivo, Mohammed, Rezika, Mueller, Ivo, Namaik-Larp, Chayadol, Newton, Paul N, Nguyen, Thuy-Nhien, Nosten, Francois, Noviyanti, Rintis, Pava, Zuleima, Pearson, Richard D, Petros, Beyene, Phyo, Aung P, Price, Ric N, Pukrittayakamee, Sasithon, Rahim, Awab Ghulam, Randrianarivelojosia, Milijaona, Rayner, Julian C, Rumaseb, Angela, Siegel, Sasha V, Simpson, Victoria J, Thriemer, Kamala, Tobon-Castano, Alberto, Trimarsanto, Hidayat, Urbano Ferreira, Marcelo, Vélez, Ivan D, Wangchuk, Sonam, Wellems, Thomas E, White, Nicholas J, William, Timothy, Yasnot, Maria F, Yilma, Daniel, AII - Infectious diseases, Intensive Care Medicine, MalariaGEN, Alam, Mohammad Shafiul [0000-0001-8330-5499], Ashley, Elizabeth [0000-0002-7620-4822], Barber, Bridget E [0000-0003-1066-7960], Batista Pereira, Dhelio [0000-0002-7761-5498], Chu, Cindy [0000-0001-9465-8214], Dondorp, Arjen M [0000-0001-5190-2395], Echeverry, Diego F [0000-0003-0301-4478], Espino, Fe [0000-0003-1690-1711], Faiz, Abdul [0000-0002-3460-7535], Golassa, Lemu [0000-0002-1216-8711], Karunaweera, Nadira [0000-0003-3985-1817], Kwiatkowski, Dominic P [0000-0002-5023-0176], Ley, Benedikt [0000-0002-5734-0845], Miotto, Olivo [0000-0001-8060-6771], Nguyen, Thuy-Nhien [0000-0002-4101-5706], Nosten, Francois [0000-0002-7951-0745], Pearson, Richard D [0000-0002-7386-3566], Phyo, Aung P [0000-0002-0383-9624], Price, Ric N [0000-0003-2000-2874], Rayner, Julian C [0000-0002-9835-1014], Urbano Ferreira, Marcelo [0000-0002-5293-9090], Wellems, Thomas E [0000-0003-3899-8454], Yasnot, Maria F [0000-0001-8081-4212], Yilma, Daniel [0000-0001-6058-2696], and Apollo - University of Cambridge Repository

Subjects
Data resource, parasitic diseases, Medicine (miscellaneous), Genomics, Genomic epidemiology, Plasmodium vivax, General Biochemistry, Genetics and Molecular Biology, Malaria
Abstract

This report describes the MalariaGEN Pv4 dataset, a new release of curated genome variation data on 1,895 samples of Plasmodium vivax collected at 88 worldwide locations between 2001 and 2017. It includes 1,370 new samples contributed by MalariaGEN and VivaxGEN partner studies in addition to previously published samples from these and other sources. We provide genotype calls at over 4.5 million variable positions including over 3 million single nucleotide polymorphisms (SNPs), as well as short indels and tandem duplications. This enlarged dataset highlights major compartments of parasite population structure, with clear differentiation between Africa, Latin America, Oceania, Western Asia and different parts of Southeast Asia. Each sample has been classified for drug resistance to sulfadoxine, pyrimethamine and mefloquine based on known markers at the dhfr, dhps and mdr1 loci. The prevalence of all of these resistance markers was much higher in Southeast Asia and Oceania than elsewhere. This open resource of analysis-ready genome variation data from the MalariaGEN and VivaxGEN networks is driven by our collective goal to advance research into the complex biology of P. vivax and to accelerate genomic surveillance for malaria control and elimination.

Published
2022

20. Additional file 2 of Reducing the risk of Plasmodium vivax after falciparum infections in co-endemic areas—a randomized controlled trial (PRIMA)

Author

Thriemer, Kamala, Degaga, Tamiru Shibru, Christian, Michael, Alam, Mohammad Shafiul, Ley, Benedikt, Hossain, Mohammad Sharif, Kibria, Mohammad Golam, Tego, Tedla Teferi, Abate, Dagimawie Tadesse, Weston, Sophie, Karahalios, Amalia, Rajasekhar, Megha, Simpson, Julie A., Rumaseb, Angela, Mnjala, Hellen, Lee, Grant, Anose, Rodas Temesgen, Kidane, Fitsum Getahun, Woyessa, Adugna, Baird, Kevin, Sutanto, Inge, Hailu, Asrat, and Price, Ric N.

Subjects
InformationSystems_INFORMATIONINTERFACESANDPRESENTATION(e.g.,HCI), ComputingMilieux_PERSONALCOMPUTING, Data_FILES, InformationSystems_MISCELLANEOUS
Abstract

Additional file 2. Participant timeline.

Published
2022
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21. Molecular surveillance over 14 years confirms reduction of Plasmodium vivax and falciparum transmission after implementation of Artemisinin-based combination therapy in Papua, Indonesia

Author

Pava, Zuleima, primary, Puspitasari, Agatha M., additional, Rumaseb, Angela, additional, Handayuni, Irene, additional, Trianty, Leily, additional, Utami, Retno A. S., additional, Tirta, Yusrifar K., additional, Burdam, Faustina, additional, Kenangalem, Enny, additional, Wirjanata, Grennady, additional, Kho, Steven, additional, Trimarsanto, Hidayat, additional, Anstey, Nicholas M., additional, Poespoprodjo, Jeanne Rini, additional, Noviyanti, Rintis, additional, Price, Ric N., additional, Marfurt, Jutta, additional, and Auburn, Sarah, additional

Published
2020
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22. Genomic Analysis of Plasmodium vivax in Southern Ethiopia Reveals Selective Pressures in Multiple Parasite Mechanisms

Author

Auburn, Sarah, primary, Getachew, Sisay, additional, Pearson, Richard D, additional, Amato, Roberto, additional, Miotto, Olivo, additional, Trimarsanto, Hidayat, additional, Zhu, Sha Joe, additional, Rumaseb, Angela, additional, Marfurt, Jutta, additional, Noviyanti, Rintis, additional, Grigg, Matthew J, additional, Barber, Bridget, additional, William, Timothy, additional, Goncalves, Sonia Morgado, additional, Drury, Eleanor, additional, Sriprawat, Kanlaya, additional, Anstey, Nicholas M, additional, Nosten, Francois, additional, Petros, Beyene, additional, Aseffa, Abraham, additional, McVean, Gil, additional, Kwiatkowski, Dominic P, additional, and Price, Ric N, additional

Published
2019
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24. No Association between the Plasmodium vivax crt-o MS334 or In9 pvcrt Polymorphisms and Chloroquine Failure in a Pre-Elimination Clinical Cohort from Malaysia with a Large Clonal Expansion.

Author

Rumaseb A, Moraes Barros RR, Sá JM, Juliano JJ, William T, Braima KA, Barber BE, Anstey NM, Price RN, Grigg MJ, Marfurt J, and Auburn S

Subjects
Humans, Chloroquine pharmacology, Chloroquine therapeutic use, Plasmodium vivax genetics, Malaysia, Drug Resistance genetics, Alleles, Protozoan Proteins genetics, Protozoan Proteins therapeutic use, Antimalarials pharmacology, Antimalarials therapeutic use, Malaria, Vivax epidemiology
Abstract

Increasing reports of resistance to a frontline malaria blood-stage treatment, chloroquine (CQ), raises concerns for the elimination of Plasmodium vivax. The absence of an effective molecular marker of CQ resistance in P. vivax greatly constrains surveillance of this emerging threat. A recent genetic cross between CQ sensitive (CQS) and CQ resistant (CQR) NIH-1993 strains of P. vivax linked a moderate CQR phenotype with two candidate markers in P. vivax CQ resistance transporter gene ( pvcrt-o ): MS334 and In9 pvcrt . Longer TGAAGH motif lengths at MS334 were associated with CQ resistance, as were shorter motifs at the In9 pvcrt locus. In this study, high-grade CQR clinical isolates of P. vivax from a low endemic setting in Malaysia were used to investigate the association between the MS334 and In9 pvcrt variants and treatment efficacy. Among a total of 49 independent monoclonal P. vivax isolates assessed, high-quality MS334 and In9 pvcrt sequences could be derived from 30 (61%) and 23 (47%), respectively. Five MS334 and six In9 pvcrt alleles were observed, with allele frequencies ranging from 2 to 76% and 3 to 71%, respectively. None of the clinical isolates had the same variant as the NIH-1993 CQR strain, and none of the variants were associated with CQ treatment failure (all P >  0.05). Multi-locus genotypes (MLGs) at 9 neutral microsatellites revealed a predominant P. vivax strain (MLG6) accounting for 52% of Day 0 infections. The MLG6 strain comprised equal proportions of CQS and CQR infections. Our study reveals complexity in the genetic basis of CQ resistance in the Malaysian P. vivax pre-elimination setting and suggests that the proposed pvcrt-o MS334 and In9 pvcrt markers are not reliable markers of CQ treatment efficacy in this setting. Further studies are needed in other endemic settings, applying hypothesis-free genome-wide approaches, and functional approaches to understand the biological impact of the TGAAGH repeats linked to CQ response in a cross are warranted to comprehend and track CQR P. vivax.

Published
2023
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25. Primaquine-induced Severe Hemolysis in the Absence of Concomitant Malaria: Effects on G6PD Activity and Renal Function.

Author

Douglas NM, Piera KA, Rumaseb A, Ley B, Anstey NM, and Price RN

Subjects
Male, Humans, Primaquine adverse effects, Hemolysis, Australia, Antimalarials adverse effects, Malaria drug therapy, Glucosephosphate Dehydrogenase Deficiency complications, Malaria, Vivax complications, Malaria, Vivax drug therapy, Malaria, Vivax prevention & control
Abstract

Primaquine prevents relapses of Plasmodium vivax malaria but can cause severe hemolysis in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. The clinical and laboratory features of this outcome are usually confounded by the clinical and hemolytic effects of concomitant malaria. We describe a case of severe hemolysis occurring after a total dose of 2.04 mg/kg of primaquine used for prophylaxis in a young, G6PD-deficient (Kaiping variant), Australian man without malaria. During acute hemolysis, he had markedly elevated urinary beta-2-microglobulin, suggestive of renal tubular injury (a well-recognized complication of primaquine-induced hemolysis). He also had albuminuria and significantly increased excretion of glycocalyx metabolites, suggestive of glomerular glycocalyx degradation and injury. We show that regularly dosed paracetamol given for its putative renoprotective effect is safe in the context of severe oxidative hemolysis. Acute drug-induced hemolysis transiently increases G6PD activity. Cases such as this improve our understanding of primaquine-induced hemolysis and ultimately will help facilitate widespread safe and effective use of this critically important drug.

Published
2022
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26. An open dataset of Plasmodium vivax genome variation in 1,895 worldwide samples.

Author

Adam I, Alam MS, Alemu S, Amaratunga C, Amato R, Andrianaranjaka V, Anstey NM, Aseffa A, Ashley E, Assefa A, Auburn S, Barber BE, Barry A, Batista Pereira D, Cao J, Chau NH, Chotivanich K, Chu C, Dondorp AM, Drury E, Echeverry DF, Erko B, Espino F, Fairhurst R, Faiz A, Fernanda Villegas M, Gao Q, Golassa L, Goncalves S, Grigg MJ, Hamedi Y, Hien TT, Htut Y, Johnson KJ, Karunaweera N, Khan W, Krudsood S, Kwiatkowski DP, Lacerda M, Ley B, Lim P, Liu Y, Llanos-Cuentas A, Lon C, Lopera-Mesa T, Marfurt J, Michon P, Miotto O, Mohammed R, Mueller I, Namaik-Larp C, Newton PN, Nguyen TN, Nosten F, Noviyanti R, Pava Z, Pearson RD, Petros B, Phyo AP, Price RN, Pukrittayakamee S, Rahim AG, Randrianarivelojosia M, Rayner JC, Rumaseb A, Siegel SV, Simpson VJ, Thriemer K, Tobon-Castano A, Trimarsanto H, Urbano Ferreira M, Vélez ID, Wangchuk S, Wellems TE, White NJ, William T, Yasnot MF, and Yilma D

Abstract

This report describes the MalariaGEN Pv4 dataset, a new release of curated genome variation data on 1,895 samples of Plasmodium vivax collected at 88 worldwide locations between 2001 and 2017. It includes 1,370 new samples contributed by MalariaGEN and VivaxGEN partner studies in addition to previously published samples from these and other sources. We provide genotype calls at over 4.5 million variable positions including over 3 million single nucleotide polymorphisms (SNPs), as well as short indels and tandem duplications. This enlarged dataset highlights major compartments of parasite population structure, with clear differentiation between Africa, Latin America, Oceania, Western Asia and different parts of Southeast Asia. Each sample has been classified for drug resistance to sulfadoxine, pyrimethamine and mefloquine based on known markers at the dhfr , dhps and mdr1 loci. The prevalence of all of these resistance markers was much higher in Southeast Asia and Oceania than elsewhere. This open resource of analysis-ready genome variation data from the MalariaGEN and VivaxGEN networks is driven by our collective goal to advance research into the complex biology of P. vivax and to accelerate genomic surveillance for malaria control and elimination., Competing Interests: No competing interests were disclosed., (Copyright: © 2022 MalariaGEN et al.)

Published
2022
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