1. Preparative separation of the diastereomers of methyl branched-chain phosphatidylcholines
- Author
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Bodo Dobner, Barbara Elsner, Frank Bringezu, Runald Stritzel, and Peter Nuhn
- Subjects
Chromatography ,Chemistry ,Organic Chemistry ,Diastereomer ,General Medicine ,Branching (polymer chemistry) ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,chemistry.chemical_compound ,Phosphatidylcholine ,Glycerol ,Separation method ,Organic chemistry ,Enantiomer - Abstract
Phosphatidylcholines with methyl branched-chain fatty acids linked to the sn-2-position of the glycerol backbone were prepared. Diastereomers were isolated if the methyl branching was at the 2-position of the fatty acids. The separation of the diastereomers was connected with the distance between the chiral centres, which was varied systematically. An increasing distance resulted in a decrease in the separation factors calculated from HPLC data. Starting the synthesis from chiral glycerol educts after separation, optically pure diastereomers of α-methyl branched chain phospholipids were obtained.
- Published
- 1996
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