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Some species of planktic foraminifera inhabiting oligotrophic surface water environments are in an obligate symbiotic relationship with dinoflagellate microalgae, which can assimilate carbon (C) through photosynthesis. However, the mechanism and dynamics of C photosynthate translocation to the foraminiferal host, and related benefits for the dinoflagellates in this symbiotic association, are poorly constrained. As a consequence, the role of planktic foraminifera as autotroph organisms in ocean surface ecosystems is not well understood. Here, we performed pulse-chase experiments with 13C-enriched dissolved inorganic carbon, followed by TEM and quantitative NanoSIMS isotopic imaging to visualize photosynthetic C assimilation by individual symbiotic dinoflagellates and subsequent translocation to their Orbulina universa host. Although most of the dinoflagellate population migrates out of the host endoplasm onto external spines during the day, our observations show that a small fraction remains inside the host cell during daytime. All symbionts, whether outside or inside the foraminifera cell, effectively assimilate C into starch nodules during daytime photosynthesis. At the onset of night, all dinoflagellates from the exterior spine–ectoplasm region migrate back into the foraminiferal cell. During the night, respiration by dinoflagellates and carbon translocation to the host, likely in the form of lipids, greatly reduces the abundance of starch in dinoflagellates. Dinoflagellate mitosis is only observed at night, with a substantial contribution of carbon fixed during the previous day contributing to the production of new biomass.

Mg/Ca ratio paleothermometry in foraminifera is an important tool for the reconstruction and interpretation of past environments. However, existing Mg/Ca:temperature relationships for planktic species inhabiting middle- and high-latitude environments are limited by a lack of information about the development and impact of low-Mg/Ca ratio “crusts” and the influence of the carbonate system on Mg/Ca ratios in these groups. To address this, we cultured individual specimens of Neogloboquadrina incompta and Neogloboquadrina pachyderma in seawater across a range of temperature (6°–12°C) and pH (7.4–8.2). We found by laser ablation inductively couple mass spectrometry analyses of shells that culture-grown crust calcite in N. incompta had a lower Mg/Ca ratio than ontogenetic calcite formed at the same temperature, suggesting that temperature is not responsible for the low-Mg/Ca ratio of neogloboquadrinid crusts. The Mg/Ca:temperature relationship for ontogenetic calcite in N. incompta was consistent with the previously published culture-based relationship, and no significant relationship was found between Mg/Ca ratios and pH in this species. However, the Mg/Ca ratio in laboratory-cultured N. pachyderma was much higher than that reported in previous core top and sediment trap samples, due to lack of crust formation in culture. Application of our ontogenetic calcite-specific Mg/Ca:temperature relationships to fossil N. pachyderma and N. incompta from five intervals in cores from the Santa Barbara Basin and the Bering Sea shows that excluding crust calcite in fossil specimens may improve Mg/Ca-based temperature estimates.

Characterizing the behavior of larvae prior to settlement is integral to understanding population dynamics because coastal oceanography may facilitate or limit settlement. Otolith microchemistry can be used to determine patterns of fish movement, although there is a limited understanding of how this tool can be applied in coastal marine systems. Our goal in this study was to evaluate the application of otolith microchemistry to characterize water mass associations of settlement-stage marine fish in a coastal upwelling region using a 3-step approach. First, we characterized seawater chemistry of coastal water mass types across multiple years, finding differences in the chemical signatures of strong upwelling, weak upwelling, and relaxation events. Second, we experimentally determined the effect of temperature on the partitioning of trace elements in otoliths for 2 rockfishes (Sebastes spp.) to find that the effect of temperature on otolith partition coefficients was element-and species-specific. Finally, we compared coeval changes in seawater and otolith chemistry of settlement-stage rockfishes that were exposed to naturally variable conditions over an upwelling-relaxation cycle. We subsequently evaluate whether laser ablation inductively coupled plasma mass spectrometry effectively measures otolith chemistry over ecologically relevant time scales. We discovered that elemental concentrations in otoliths respond rapidly to changes in seawater chemistry and reflect equivalent proportional changes. This study provides evidence that elemental signatures are valuable tools for reconstructing larval histories of marine fish in coastal upwelling regions.

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is becoming a widespread technique for analyzing elemental ratios in foraminiferal calcite. Here we focus on optimizing LA-ICP-MS for high-resolution depth profiling of elemental ratios through shell walls. This application reveals intrashell variability and provides a unique opportunity to quantify trace element incorporation over short time scales of calcification by an individual foraminifer. High-resolution depth profiling requires careful consideration of both ablation and analytical conditions required to resolve differences in shell chemistry across sub-micron shell thickness. We present laser ablation profiles of NIST SRM 610 standard glass data (in cps) and elemental/Ca ratios (in mmol/mol) from foraminiferal calcite obtained over a range of operating conditions using a Photon Machines 193nm UV excimer laser-ablation system, equipped with a dual-volume ANU HelEx chamber, coupled to an Agilent 7700x quadrupole ICP-MS. Different combinations of energy density, repetition rate, and mass spectrometer cycle time can yield varying elemental profiles. This variability can mimic and/or mask real intrashell trace element heterogeneity in foraminifer shells. At low (

Geochemical observations indicate that planktic foraminifer test Mg/Ca is heterogeneous in many species, thereby challenging its use as a paleotemperature proxy for paleoceanographic reconstructions. We present Mg/Ca and Ba/Ca data collected by laser ablation ICP-MS from the shells of Orbulina universa cultured in controlled laboratory experiments. Test calcite was labeled with Ba-spiked seawater for 12 h day or night calcification periods to quantify the timing of intratest Mg-banding across multiple diurnal cycles. Results demonstrate that high Mg bands are precipitated during the night whereas low Mg bands are precipitated during the day. Data obtained from specimens growing at 20 °C and 25 °C show that Mg/Ca ratios in both high and low Mg bands increase with temperature, and average test Mg/Ca ratios are in excellent agreement with previously published empirical calibrations based on bulk solution ICP-MS analyses. In general, Mg band concentrations decrease with increasing pH and/or [CO2-3] but this effect decreases as experimental temperatures increase from 20 °C to 25 °C. We suggest that mitochondrial uptake of Mg2+ from the thin calcifying fluid beneath streaming rhizopodial filaments may provide the primary locus for Mg2+ removal during test calcification, and that diurnal variations in either mitochondrial density or activity produce Mg banding. These results demonstrate that Mg banding is an inherent component of test biomineralization in O. universa and show that the Mg/Ca paleothermometer remains a fundamental tool for reconstructing past ocean temperatures from fossil foraminifers.

Willows (Salix SPP.) are increasingly being grown as a source of biomass which can be harnessed for energy production at a commercial scale as one example of a Short Rotation Coppice (SRC) crop. What is less widely understood is the role of the plants in reducing nutrient loads when planted as a nature-based solution to mitigate diffuse pollution from agricultural lands. Strategically planted woodland and forest can play a major role in Nature Based Solutions (NBS) approaches. SRC willows were planted in a 22-ha grassland micro-catchment located on a research farm in Northern Ireland, which is regularly grazed by cattle and sheep. The micro-catchment comprises a small ditch with upstream and downstream monitoring points where a pair of autosamplers were used to collect water samples for nutrient analysis. It was possible to reconstruct a timeseries of hourly discharge in the ungauged micro-catchment using the SHETRAN hydrological model. Subsequently, the modelled flows were used to estimate daily phosphorus (P) loads at both upstream and downstream monitoring points from the measured P concentrations. The nearly four years (June 2018–April 2022) of monitoring data allowed P loads to be calculated for pre-planting, juvenile trees and mature trees phases. A baseline scenario was run without any changes to the model; this evaluated the changes in P loads from the catchment. A further Post-Willows scenario “P–W” modified some model parameters and evapotranspiration to represent the land use change due to the willow plantation. The results from the baseline were evaluated and showed that (i) there was a statistically significant reduction in particulate P (PP) and total P (TP) concentrations at the outlet of the micro-catchment; (ii) a statistically significant reduction in PP and TP loads, indicating that the willows were reducing PP export (losses) probably by trapping fine sediment in the ditch. Results from the P–W scenario showed a smaller than expected decrease in discharge from the model results. The overall export of TP from the micro-catchment could be further decreased by additional planting of SRC willows, but the export of soluble forms of P did not decrease by a significant amount post-planting which is a recommendation for further study in mitigation programmes. The use of SRC willows in riparian buffer strips (RBS) which are a common form of NBS, has shown promising results in terms of trapping particulate forms of P. The layout of the RBS should also be carefully designed in terms of maximising the buffer strip width, here the areas planted was optimised to make best use of unproductive farmland. If the RBS can be designed to maximise the interception of runoff flowing downhill from agricultural fields then the removal rate should be even higher.

Abstract Background Persisting symptoms after concussion (PSaC) include physical, cognitive, and psychological symptoms which contribute to rehabilitation challenges. Previous research has not thoroughly investigated the association between PSaC and pain‐related psychological factors. Therefore, there is an opportunity to use current pain models, such as the Fear Avoidance Model (FAM), as a framework to explore these relationships. The goals of this integrative review are to (1) identify and describe range of evidence that explores relationships between psychological factors and clinical outcomes in patients with PSaC, and (2) develop a comprehensive understanding of FAM‐specific psychological factors that have been identified as potential predictors of clinical outcomes in patients with PSaC. Methods This review will be based on principles and stages of an integrative review which will allow for inclusion of diverse methodologies: (1) problem formulation, (2) literature search, (3) data evaluation, (4) data analysis, and (5) presentation. Methods for reporting this review will be informed by the 2020 PRISMA guidelines for systematic reviews. Discussion The findings from this integrative review will inform healthcare professionals working in post‐concussion rehabilitation settings regarding relationships between FAM psychological factors and PSaC—an area that until recently has not been thoroughly explored. Additionally, this review will inform the development of other reviews and clinical studies to further investigate relationships between FAM psychological factors and PSaC. Integrative Review Registration OSF DOI 10.17605/OSF.IO/CNGPW.

Catchment models are essential tools to identify and predict water quality problems linked to excessive nutrient applications (in this case phosphorus (P)). The Catchment Runoff Attenuation Flux Tool (CRAFT) has been successfully used to model nutrient fluxes and concentrations in north-western European catchments. The model is extremely parsimonious due to the relatively small number of parameters. However, an improvement to the representation of soluble P and particulate P fluxes in the fast-subsurface and surface runoff flow pathways was required. A case study in the north of Ireland applied the original and the new, enhanced (Dynamic) version of the CRAFT to the trans-border Blackwater catchment (UK and Republic of Ireland) covering nearly 1500 km2, with the land use predominantly livestock grazing. The larger size of the Blackwater also required a nested modeling approach to be implemented using a multiple sub-catchment variant (MultiCRAFT). P load reductions in the different sub-catchments were first identified using a simple approach based on the gap between the Water Framework Directive (WFD) limits for “Good” ecological status for soluble reactive P (SRP) concentrations and the recently observed concentrations. Modeling of different mitigation scenarios was then conducted using the MultiCRAFT framework with the best-performing variant of the CRAFT model embedded. The catchment was found to have flashy, episodic delivery of high concentrations of SRP and PP during runoff events which will require different sources (i.e., diffuse and point) of P to be targeted to achieve the WFD targets by the end of the decade. The modeling results thus showed that the required SRP load reductions could be best achieved using a combined scenario of mitigation measures that targeted diffuse sources contributing to both the surface runoff and fast-subsurface flow pathways, with point sources also identified as needing reduction in some sub-catchments.

As our climate continues to respond to anthropogenic forcing, the magnitude and frequency of individual weather events and the intensity of the weather extremes associated with these, remains highly uncertain. This is a particular concern for our infrastructure networks, as increasing storm-related damage to these vital lifelines has significant consequences for our communities. Effective first response is hence becoming an increasingly important part of the management of infrastructure systems. Here, we propose a novel and rational framework for ‘consequence forecasting’ that enables probabilistic, pre-event decision-making for first responders to effectively target resources prior to an extreme weather event and thus reduce the societal consequences. Our method is unique in that it minimises model bias by using the same numerical weather prediction model for both fault attribution and fault prediction. Our framework can predict failure rates that are within 50% of the true value for more than 50% of the events considered, some 24 h in advance, therefore demonstrating that it can be an important part of increasing societal climate resilience by reducing reinstatement times.

A water retention curve (WRC) enables the quick determination of suction in a soil or tailings using the gravimetric water content or degree of saturation. However, establishing the WRC is reliant on time consuming laboratory testing. Here an empirical method is presented that allows the WRC to be estimated quickly, including its void ratio dependency, by relating the fractal and other characteristics of the soil or tailings particle size distributions (PSD) to the parameters which define the WRC. The defining parameters, from a fractal mathematics viewpoint, depend on the particle and pore surface areas and shapes as well as their fractal dimensions. More generally, the clay percentage must also have an important role as it has a major influence on these properties, especially the surface areas. Twelve WRCs for soils and tailings were compiled and compared and used to establish the new empirical expressions. Estimated WRCs using these new empirical expressions compare well with those fitted directly to experimental results. Guidelines are also given on how to quantify suction’s contribution to strength using the location of the hydraulic state on the WRC, adding to the practical appeal of this work.

Instabilities mostly happen in fully saturated and loose non-cohesive geomaterials like sands or silts or tailings, but it is also possible in unsaturated geomaterials. When unsaturated they can experience a reduction in effective stress and strain soften during water (and air) undrained loading, attaining a very low residual strength. This study focuses on modelling the conditions required to cause instability in unsaturated silty tailings, giving particular consideration to the presence of air and the way it alters the ability for volume change when it remains trapped inside the tailings. A gold tailings is used to calibrate the UNSW boundingsurface plasticity model. The effect of air, including the volumetric change caused by air compression, the alteration of air pressure, the contribution of suction to the effective stress, and suction hardening, are explored. Collapse lines (sometimes referred to as instability lines or flow liquefaction lines which represent boundaries between stable and potentially unstable stress states) in the 𝑞𝑞 − 𝑝𝑝′ plane are explored. The undrained shear strength ratios and slopes of the collapse lines are compared to those of other tailings andsands when unsaturated.

Laboratory-controlled cone penetration test results of a silty gold tailings in a variety of saturated and unsaturated states, obtained using two calibration chambers, are presented then interpreted using a state parameter-based approach. The research was conducted as part of the TAILLIQ project (tailliq.com), funded by the Australian Research Council and six mining companies, and involving four Australian universities.Cone penetration resistances, which increase due to the presence of suction when the tailings are unsaturated, can be normalised using the initial mean effective stress to establish a relationship with the initial state parameter. The relationship is applicable to saturated and unsaturated conditions, as long as the presence of suction hardening as well as the influence of suction on the mean effective stress are accounted for, and as long as the cone penetrations occur under pseudo drained conditions. The relationships enable state parameters and void ratios to be back-calculated from normalised cone penetration resistances.

Unsaturated loess and loess-derived soils in the Akaroa harbour area of New Zealand are vulnerable to shallow landsliding during rainfall events. Laboratory testing and long-term field instrumentation has been conducted to characterise the water retention and unsaturated shear strength of these materials, and better understand temporal changings in slope stability. Laboratory test results indicate that the same soil-water characteristic curve can be applied to both recompacted and intact loess when suction is normalised by the air entry value. Conversely the stress-strain behaviours of the recompacted and intact loess were different due to the unique microstructure of the intact loess that contributes to its shear strength. Long-term field instrumentation data showed that, for the duration of the monitoring period, the hydraulic state of the loess remained on a scanning curve. These data, combined with the laboratory testing, confirm that temporal variation in slope stability can be attributed to seasonal variability in suction and its contribution to unsaturated shear strength. These hydromechanical variabilities, resulting from wetting and drying, are affected by rainfall intensity and duration that occurs at the site.

Shallow rainfall triggered slope failures occur frequently in loess and loess-derived deposits across the South Island, New Zealand. These failures, which occur in both natural slopes and engineered cuttings, impact road infrastructure, residential housing and rural land use. When dry, the loess can form near vertical cuttings. However, with increase in moisture content loess slopes become susceptible to shallow slope failures. To date, the influence of negative pore-water pressure (suction) on the stability of loess slopes in New Zealand has not been well understood. In this paper, data from long term in situ field monitoring of rainfall, suction and volumetric water content from a loess slope in Banks Peninsula, Canterbury are presented with laboratory triaxial test results undertaken on undisturbed unsaturated loess samples. Field and laboratory soil responses to wetting and drying are compared, and the characteristics of rainfall events which reduce suction in situ and therefore slope stability are discussed.

Internal erosion (suffusion) is caused by water seeping through the matrix of coarse soil and progressively transporting out fine particles. The mechanical strength of soils within water retaining structures may be affected after internal erosion occurs. However, most experimental investigations on the mechanical consequences of internal erosion have used triaxial tests on samples having nonhomogeneous particle size distributions along their lengths. Such nonhomogeneities arise from the most commonly used sample formation procedure, in which seeping water enters one end of a sample and washes fine particles out the other. In this paper a new soil sample formation procedure is presented which results in homogeneous particle size distributions along the direction of seepage, that is at all locations along a sample's length.

An important issue surrounding the identification of liquefaction susceptibility using laboratory testing is how well the soil sample being tested represents the soil in the field. Undisturbed samples are difficult and costly to obtain, while reconstituted soil samples must have a structure and fabric that represents in situ conditions as closely as possible. Recent laboratory tests on sand samples revealed that liquefaction resistance is strongly affected by the sample preparation technique, as different techniques result in different fabrics and structures. The same may be true for silty sand samples, although they have not been given the same research attention. Thus, this paper presents cyclic triaxial test results on non-plastic silty sand samples (a decomposed granite) and determines the number of cycles required to cause liquefaction, considering different failure criterion as well as different confining pressures, cyclic strength ratios and sample preparation techniques. The techniques include dry and slurry deposition. The experimental results show that the preparation technique does not have a significant influence on cyclic resistance.

Synthetic fibres may be used to reinforce soils. Fibre reinforcement may, for example, improve the mechanical behaviour of very loose sand which is usually susceptible to static liquefaction. In this study, two types of polypropylene fibres are mixed into sand to explore the effect of fibre reinforcement on drained volumetric behaviour and undrained static liquefaction. Drained and undrained stress-controlled triaxial compression tests are conducted on both unreinforced and fibre reinforced samples which are in very loose states. It is observed that, under drained compression, both unreinforced and fibre reinforced samples show volumetric contraction. In undrained compression the excess pore water pressure eventually becomes almost equal to the initial confining stress in all samples. This represents a state of liquefaction in unreinforced samples, and they become fluidised indicating the effective stress has become zero. However, in reinforced samples, the fluidised condition is absent, indicating that a conventional type of liquefaction has not occurred. It is concluded that static liquefaction in very loose sand can be prevented by fibre reinforcement, as the induced tensile stress in fibres makes the effective stress (that is the stress carried by the soil skeleton) remain above zero even when the excess pore water pressure is equal to the confining stress.

Up to 89% of patients with herpes simplex virus type-1 (HSV-1) encephalitis can have seizures. Possibly, viruses are environmental triggers for seizures in genetically vulnerable individuals. Inherited dynorphin promoter polymorphisms are associated with temporal lobe epilepsy and febrile seizures in man. In animals, the dynorphin system in the hippocampus regulates excitability. The hypothesis that reduced dynorphin expression in dentate gyrus of hippocampus due to HSV-1 infection leads to epileptic responses was tested in a rat model of HSV-1 encephalitis using EEG recording, histopathological and neuropharmacologic probes. HSV-1 infection causes loss of dynorphin A-like immunoreactivity in hippocampus, an effect independent of direct viral interference and cell loss. A kappa opioid receptor agonist U50488 effectively blocks ictal activity, linking absence of dynorphin to propensity for epileptic activity. These findings show a vulnerability of hippocampal dynorphin during infection, suggesting a neurochemical basis for seizures that may be generalizable to other encephalitic viruses.

This paper considers the anisotropy associated with the interparticle contact network and interconnected pore space, which is quantified through a scalar quantity based on the fabric tensor. The study focused on the anisotropy associated with subsets of contacts and pores. It is shown that the stress ratio can be directly related to the geometric anisotropy of strong and non-sliding contacts, and that the anisotropy of pores is correlated with the anisotropy of contacts.

Very little is known about how to interpret the cone penetration test (CPT) when performed in unsaturated soils. The few published studies on the CPT in unsaturated soils have focused on either clean sands or a silt. In this study new results of laboratory-controlled CPTs in an unsaturated silty sand are presented. The silty sand exhibits hydraulic hysteresis and suction hardening. Suction is observed to have a pronounced affect on measured cone penetration resistance. For an isotropic net confining stress of 60 kPa it is observed that higher suctions give rise to cone penetration resistances that are 50% larger than those for lower suctions. A semi-theoretical correlation is presented that links measured cone penetration resistances to initial relative density and mean effective stress. For this silty sand it is shown that failing to account for suction may result in significant overestimations and unsafe predictions of soil properties from measured cone penetration resistances.

The bearing capacity of strip footings on unsaturated soils is studied using slip line theory. The suction profiles considered are non-uniform with depth and correspond to vertical flow of water by infiltration or evaporation. The slip line theory assumes a plastic equilibrium state of a Mohr-Coulomb soil in which suction influences are included using the effective stress concept. This paper shows that the influence of a non-uniform suction profile on bearing capacity is significant. When the contribution of suction to the effective stress can be approximated as a function that varies linearly with depth, the effects of suction on bearing capacity can be represented in dimensionless form in separate charts for smooth and rough footings. Using the charts the bearing capacity can be determined for any combination of friction angle, footing width, surcharge, soil unit weight and linear profiles of cohesion and the contribution of suction to the effective stress.

Background: Opportunities for home-monitoring are increasing exponentially. Home- spirometry is reproducible and reliable in interstitial lung disease (ILD), yet patients' experiences are not reported. Given the morbidity and mortality associated with ILDs, maintaining health-related quality-of-life is vital. We report our findings from a codesigned, qualitative study capturing the perspectives and experiences of patients using home-spirometry in a UK regional ILD National Health Service England (NHSE) commissioned service., Methods: Patients eligible for home-spirometry as routine clinical care, able to give consent and able to access a smart phone were invited to participate. In-depth, semistructured interviews were conducted at serial time points (baseline, 1, 3 and 6 months), recorded, transcribed and analysed thematically., Results: We report on the experiences of 10 recruited patients (8 males; median age 66 years, range 50-82 years; 7 diagnosed with idiopathic pulmonary fibrosis, 3 other ILDs) who generally found spirometry convenient and easy to use, but their relationships with forced vital capacity results were complex. Main themes emerging were: (1) anticipated benefits-to identify change, trigger action and aid understanding of condition; (2) needs-clinical oversight and feedback, understanding of results, ownership, need for data and a need 'to know'; (3) emotional impact-worry, reassurance, ambivalence/conflicting feelings, reminder of health issues, indifference; (4) ease of home-spirometry-simplicity, convenience and (5) difficulties with home-spirometry-technical issues, technique, physical effort., Conclusion: Home-spirometry has many benefits, but in view of the potential risks to psychological well-being, must be considered on an individual basis. Informed consent and decision-making are essential and should be ongoing, acknowledging potential limitations as well as benefits. Healthcare support is vital., Competing Interests: Competing interests: JM and JL are supported by NIHR funding to undertake this work. SL received honorarium from Boerhinger Ingelheim; MN received speaker fees from Boehringer-Ingelheim, AstraZeneca and Hoffman La Roche and advisory board payments from Boehringer Ingelheim. MN is co-chair of Interstitial Lung Disease Interdisciplinary Network (unpaid) and chair of the Interstitial Lung Disease Pharmacist Network (paid). JL is in receipt of grants from European Respiratory Society, AstraZeneca and GlaxoSmithKline. AMR received speaker fees from Boehringer-Ingelheim, Hoffman La Roche and the Irish Lung Fibrosis Association. AMR is a National Institute for Health Research (NIHR) Senior Research Leader., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY. Published by BMJ.)

The spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), an invasive planthopper discovered in Pennsylvania, U.S. in 2014, has spread to many surrounding states despite quarantines and control efforts, and further spread is anticipated. A classical (importation) biological control program would contribute to the long-term management of L. delicatula in the eastern U.S. In its native range of China, Anastatus orientalis (Hymenoptera: Eupelmidae), an egg parasitoid, causes significant mortality. Anastatus orientalis consists of multiple haplotypes that differ in important biological parameters. To delineate the physiological host range of A. orientalis Haplotype C, we completed no-choice and choice testing. No-choice testing of non-target eggs from 36 insect species spanning six orders and 18 families showed that physiologically this haplotype of A. orientalis can develop in a variety of host species eggs from the families Coreidae, Fulgoridae, Pentatomidae, and Saturniidae. Ten of the 16 species that were attacked in the no-choice tests were also attacked in the choice tests. The production of progeny on non-target egg masses was significantly lower than on the controls ( L. delicatula egg masses run simultaneously) in the no-choice and choice tests. For the non-target species that were attacked and resulted in female wasp progeny, these females were able to produce their own progeny at the same rate as control females that were reared from the L. delicatula eggs. Larger host eggs corresponded to an increased female-biased sex ratio of the progeny, suggesting that gravid females select them for fertilized eggs. Results from these studies suggest that A. orientalis Haplotype C prefers to parasitize L. delicatula egg masses but is capable of developing in some non-target species., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Broadley, Sipolski, Pitt, Hoelmer, Wang, Cao, Tewksbury, Hagerty, Bartlett, Russell, Wu, Davis, Kaser, Elkinton and Gould.)

The biodegradable, aliphatic polyester poly(lactic acid), PLA, is a leading bio-based alternative to petrochemical-derived plastic materials across a range of applications. Widely reported in the available literature as a benchmark for PLA production via the bulk ring-opening polymerization of lactides is the use of divalent tin catalysts, and particularly tin(II) bis(2-ethylhexanoate). We present an alternative zirconium-based system that combines an inexpensive Group IV metal with the robustness, high activity, control, and designed compatibility with existing facilities and processes, that are required for industrial use. We have carried out a comprehensive kinetic study and applied a combined experimental and theoretical approach to understanding the mechanism by which the polymerization of lactide proceeds in the presence of this system. In the laboratory-scale (20 g) polymerization of recrystallized racemic d,l-lactide ( rac -lactide), we have measured catalyst turnover frequencies up to at least 56,000 h -1 , and confirmed the reported protocols' resistance toward undesirable epimerization, transesterification, and chain scission processes, deleterious to the properties of the polymer product. Further optimization and scale-up under industrial conditions have confirmed the relevance of the catalytic protocol to the commercial production of melt-polymerized PLA. We were able to undertake the efficient preparation of high-molecular-weight PLA on the 500-2000 g scale, via the selective and well-controlled polymerization of commercial polymer-grade l-lactide under challenging, industrially relevant conditions, and at metal concentrations as low as 8-12 ppm Zr by weight ([Zr] = 1.3 × 10 -3 to 1.9 × 10 -3 mol %). Under those conditions, a catalyst turnover number of at least 60,000 was attained, and the activity of the catalyst was comparable to that of tin(II) bis(2-ethylhexanoate)., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

This Letter presents the results from the MiniBooNE experiment within a full "3+1" scenario where one sterile neutrino is introduced to the three-active-neutrino picture. In addition to electron-neutrino appearance at short baselines, this scenario also allows for disappearance of the muon-neutrino and electron-neutrino fluxes in the Booster Neutrino Beam, which is shared by the MicroBooNE experiment. We present the 3+1 fit to the MiniBooNE electron-(anti)neutrino and muon-(anti)neutrino data alone and in combination with MicroBooNE electron-neutrino data. The best-fit parameters of the combined fit with the exclusive charged-current quasielastic analysis (inclusive analysis) are Δm^{2}=0.209  eV^{2}(0.033  eV^{2}), |U_{e4}|^{2}=0.016(0.500), |U_{μ4}|^{2}=0.500(0.500), and sin^{2}(2θ_{μe})=0.0316(1.0). Comparing the no-oscillation scenario to the 3+1 model, the data prefer the 3+1 model with a Δχ^{2}/d.o.f.=24.7/3(17.3/3), a 4.3σ(3.4σ) preference assuming the asymptotic approximation given by Wilks's theorem.

Nitrogen availability often limits biological productivity in marine systems, where inorganic nitrogen, such as ammonium is assimilated into the food web by bacteria and photoautotrophic eukaryotes. Recently, ammonium assimilation was observed in kleptoplast-containing protists of the phylum foraminifera, possibly via the glutamine synthetase/glutamate synthase (GS/GOGAT) assimilation pathway imported with the kleptoplasts. However, it is not known if the ubiquitous and diverse heterotrophic protists have an innate ability for ammonium assimilation. Using stable isotope incubations ( 15 N-ammonium and 13 C-bicarbonate) and combining transmission electron microscopy (TEM) with quantitative nanoscale secondary ion mass spectrometry (NanoSIMS) imaging, we investigated the uptake and assimilation of dissolved inorganic ammonium by two heterotrophic foraminifera; a non-kleptoplastic benthic species, Ammonia sp., and a planktonic species, Globigerina bulloides . These species are heterotrophic and not capable of photosynthesis. Accordingly, they did not assimilate 13 C-bicarbonate. However, both species assimilated dissolved 15 N-ammonium and incorporated it into organelles of direct importance for ontogenetic growth and development of the cell. These observations demonstrate that at least some heterotrophic protists have an innate cellular mechanism for inorganic ammonium assimilation, highlighting a newly discovered pathway for dissolved inorganic nitrogen (DIN) assimilation within the marine microbial loop., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Bird, LeKieffre, Jauffrais, Meibom, Geslin, Filipsson, Maire, Russell and Fehrenbacher.)

The symbiotic planktonic foraminifera Orbulina universa inhabits open ocean oligotrophic ecosystems where dissolved nutrients are scarce and often limit biological productivity. It has previously been proposed that O. universa meets its nitrogen (N) requirements by preying on zooplankton, and that its symbiotic dinoflagellates recycle metabolic 'waste ammonium' for their N pool. However, these conclusions were derived from bulk 15 N-enrichment experiments and model calculations, and our understanding of N assimilation and exchange between the foraminifer host cell and its symbiotic dinoflagellates remains poorly constrained. Here, we present data from pulse-chase experiments with 13 C-enriched inorganic carbon, 15 N-nitrate, and 15 N-ammonium, as well as a 13 C- and 15 N- enriched heterotrophic food source, followed by TEM (transmission electron microscopy) coupled to NanoSIMS (nanoscale secondary ion mass spectrometry) isotopic imaging to visualize and quantify C and N assimilation and translocation in the symbiotic system. High levels of 15 N-labelling were observed in the dinoflagellates and in foraminiferal organelles and cytoplasm after incubation with 15 N-ammonium, indicating efficient ammonium assimilation. Only weak 15 N-assimilation was observed after incubation with 15 N-nitrate. Feeding foraminifers with 13 C- and 15 N-labelled food resulted in dinoflagellates that were labelled with 15 N, thereby confirming the transfer of 15 N-compounds from the digestive vacuoles of the foraminifer to the symbiotic dinoflagellates, likely through recycling of ammonium. These observations are important for N isotope-based palaeoceanographic reconstructions, as they show that δ 15 N values recorded in the organic matrix in symbiotic species likely reflect ammonium recycling rather than alternative N sources, such as nitrates.

Polymetallocenes based on ferrocene, and to a lesser extent cobaltocene, have been well-studied, whereas analogous systems based on nickelocene are virtually unexplored. It has been previously shown that poly(nickelocenylpropylene) [Ni(η 5 -C 5 H 4 ) 2 (CH 2 ) 3 ] n is formed as a mixture of cyclic (6 x ) and linear (7) components by the reversible ring-opening polymerisation (ROP) of tricarba[3]nickelocenophane [Ni(η 5 -C 5 H 4 ) 2 (CH 2 ) 3 ] (5). Herein the generality of this approach to main-chain polynickelocenes is demonstrated and the ROP of tetracarba[4]nickelocenophane [Ni(η 5 -C 5 H 4 ) 2 (CH 2 ) 4 ] (8), and disila[2]nickelocenophane [Ni(η 5 -C 5 H 4 ) 2 (SiMe 2 ) 2 ] (12) is described, to yield predominantly insoluble homopolymers poly(nickelocenylbutylene) [Ni(η 5 -C 5 H 4 ) 2 (CH 2 ) 4 ] n (13) and poly(tetramethyldisilylnickelocene) [Ni(η 5 -C 5 H 4 ) 2 (SiMe 2 ) 2 ] n (14), respectively. The ROP of 8 and 12 was also found to be reversible at elevated temperature. To access soluble high molar mass materials, copolymerisations of 5, 8, and 12 were performed. Superconducting quantum interference device (SQUID) magnetometry measurements of 13 and 14 indicated that these homopolymers behave as simple paramagnets at temperatures greater than 50 K, with significant antiferromagnetic coupling that is notably larger in carbon-bridged 6 x /7 and 13 compared to the disilyl-bridged 14. However, the behaviour of these polynickelocenes deviates substantially from the Curie-Weiss law at low temperatures due to considerable zero-field splitting., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

The MiniBooNE experiment at Fermilab reports results from an analysis of ν_{e} appearance data from 12.84×10^{20} protons on target in neutrino mode, an increase of approximately a factor of 2 over previously reported results. A ν_{e} charged-current quasielastic event excess of 381.2±85.2 events (4.5σ) is observed in the energy range 200

We report the first measurement of monoenergetic muon neutrino charged current interactions. MiniBooNE has isolated 236 MeV muon neutrino events originating from charged kaon decay at rest (K^{+}→μ^{+}ν_{μ}) at the NuMI beamline absorber. These signal ν_{μ}-carbon events are distinguished from primarily pion decay in flight ν_{μ} and ν[over ¯]_{μ} backgrounds produced at the target station and decay pipe using their arrival time and reconstructed muon energy. The significance of the signal observation is at the 3.9σ level. The muon kinetic energy, neutrino-nucleus energy transfer (ω=E_{ν}-E_{μ}), and total cross section for these events are extracted. This result is the first known-energy, weak-interaction-only probe of the nucleus to yield a measurement of ω using neutrinos, a quantity thus far only accessible through electron scattering.

We describe two new types of reactivity involving silicon-bridged [1]ferrocenophanes. In an attempt to form a [1]ferrocenophane with a bridging silyl cation, the reaction of sila[1]ferrocenophane [Fe(η-C 5 H 4 ) 2 Si(H)TMP] (12) (TMP = 2,2,6,6-tetramethylpiperidyl) towards the hydride-abstraction reagent trityl tetrakis(pentafluorophenyl)borate ([CPh 3 ][B(C 6 F 5 ) 4 ]) was explored. This yielded the unusual dinuclear species [Fe(η-C 5 H 4 ) 2 Si(TMP·H)(η-C 5 H 3 )Fe(η-C 5 H 4 )Si(H)TMP][B(C 6 F 5 ) 4 ] [13][B(C6F5)4] in low yield. The formation of [13]+ is proposed to involve abstraction of hydride from the silicon bridge in 12 with subsequent C-H bond cleavage of a cyclopentadienyl group by the resulting electrophilic transient silyl cation intermediate. We also explored the reaction of dimethylsila[1]ferrocenophane [Fe(η-C 5 H 4 ) 2 SiMe 2 ] (1) with the Au(i) species AuCl(PMe 3 ). This was found to result in addition of the Au-Cl bond across the Cp ipso -Si bond to yield the ring-opened species [1'-(chlorodimethylsilyl)-ferrocenyl](trimethylphosphine)gold(i), [Fe(C 5 H 4 SiMe 2 Cl){C 5 H 4 Au(PMe 3 )}] (14). This represents the first example of ring-opening addition of a metallocenophane with a reagent possessing a transition metal-halogen bond.

Uncovering the complexities of trophic and metabolic interactions among microorganisms is essential for the understanding of marine biogeochemical cycling and modelling climate-driven ecosystem shifts. High-throughput DNA sequencing methods provide valuable tools for examining these complex interactions, although this remains challenging, as many microorganisms are difficult to isolate, identify and culture. We use two species of planktonic foraminifera from the climatically susceptible, palaeoceanographically important genus Neogloboquadrina, as ideal test microorganisms for the application of 16S rRNA gene metabarcoding. Neogloboquadrina dutertrei and Neogloboquadrina incompta were collected from the California Current and subjected to either 16S rRNA gene metabarcoding, fluorescence microscopy, or transmission electron microscopy (TEM) to investigate their species-specific trophic interactions and potential symbiotic associations. 53-99% of 16S rRNA gene sequences recovered from two specimens of N. dutertrei were assigned to a single operational taxonomic unit (OTU) from a chloroplast of the phylum Stramenopile. TEM observations confirmed the presence of numerous intact coccoid algae within the host cell, consistent with algal symbionts. Based on sequence data and observed ultrastructure, we taxonomically assign the putative algal symbionts to Pelagophyceae and not Chrysophyceae, as previously reported in this species. In addition, our data shows that N. dutertrei feeds on protists within particulate organic matter (POM), but not on bacteria as a major food source. In total contrast, of OTUs recovered from three N. incompta specimens, 83-95% were assigned to bacterial classes Alteromonadales and Vibrionales of the order Gammaproteobacteria. TEM demonstrates that these bacteria are a food source, not putative symbionts. Contrary to the current view that non-spinose foraminifera are predominantly herbivorous, neither N. dutertrei nor N. incompta contained significant numbers of phytoplankton OTUs. We present an alternative view of their trophic interactions and discuss these results within the context of modelling global planktonic foraminiferal abundances in response to high-latitude climate change.

Anthropogenically-forced changes in ocean chemistry at both the global and regional scale have the potential to negatively impact calcifying plankton, which play a key role in ecosystem functioning and marine carbon cycling. We cultured a globally important calcifying marine plankter (the foraminifer, Globigerina bulloides) under an ecologically relevant range of seawater pH (7.5 to 8.3 total scale). Multiple metrics of calcification and physiological performance varied with pH. At pH > 8.0, increased calcification occurred without a concomitant rise in respiration rates. However, as pH declined from 8.0 to 7.5, calcification and oxygen consumption both decreased, suggesting a reduced ability to precipitate shell material accompanied by metabolic depression. Repair of spines, important for both buoyancy and feeding, was also reduced at pH < 7.7. The dependence of calcification, respiration, and spine repair on seawater pH suggests that foraminifera will likely be challenged by future ocean conditions. Furthermore, the nature of these effects has the potential to actuate changes in vertical transport of organic and inorganic carbon, perturbing feedbacks to regional and global marine carbon cycling. The biological impacts of seawater pH have additional, important implications for the use of foraminifera as paleoceanographic indicators.

The relationship between seawater temperature and the average Mg/Ca ratios in planktic foraminifera is well established, providing an essential tool for reconstructing past ocean temperatures. However, many species display alternating high and low Mg-bands within their shell walls that cannot be explained by temperature alone. Recent experiments demonstrate that intrashell Mg variability in Orbulina universa, which forms a spherical terminal shell, is paced by the diurnal light/dark cycle. Whether Mg-heterogeneity is also diurnally paced in species with more complex shell morphologies is unknown. Here we show that high Mg/Ca-calcite forms at night in cultured specimens of the multi-chambered species Neogloboquadrina dutertrei. Our results demonstrate that N. dutertrei adds a significant amount of calcite, and nearly all Mg-bands, after the final chamber forms. These results have implications for interpreting patterns of calcification in N. dutertrei and suggest that diurnal Mg-banding is an intrinsic component of biomineralization in planktic foraminifera.

Plankton, corals, and other organisms produce calcium carbonate skeletons that are integral to their survival, form a key component of the global carbon cycle, and record an archive of past oceanographic conditions in their geochemistry. A key aspect of the formation of these biominerals is the interaction between organic templating structures and mineral precipitation processes. Laboratory-based studies have shown that these atomic-scale processes can profoundly influence the architecture and composition of minerals, but their importance in calcifying organisms is poorly understood because it is difficult to measure the chemistry of in vivo biomineral interfaces at spatially relevant scales. Understanding the role of templates in biomineral nucleation, and their importance in skeletal geochemistry requires an integrated, multiscale approach, which can place atom-scale observations of organic-mineral interfaces within a broader structural and geochemical context. Here we map the chemistry of an embedded organic template structure within a carbonate skeleton of the foraminifera Orbulina universa using both atom probe tomography (APT), a 3D chemical imaging technique with Ångström-level spatial resolution, and time-of-flight secondary ionization mass spectrometry (ToF-SIMS), a 2D chemical imaging technique with submicron resolution. We quantitatively link these observations, revealing that the organic template in O. universa is uniquely enriched in both Na and Mg, and contributes to intraskeletal chemical heterogeneity. Our APT analyses reveal the cation composition of the organic surface, offering evidence to suggest that cations other than Ca 2+ , previously considered passive spectator ions in biomineral templating, may be important in defining the energetics of carbonate nucleation on organic templates., Competing Interests: The authors declare no conflict of interest.