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152 results on '"Rustichelli Franco"'

10. Binding of the major phasin, PhaP1, from Ralstonia eutropha H16 to poly(3-hydroxybutyrate) granules

Author

Neumann, Liv, Spinozzi, Francesco, Sinibaldi, Raffaele, Rustichelli, Franco, Potter, Markus, and Steinbuchel, Alexander

Subjects
Protein binding -- Research, Burkholderia -- Physiological aspects, Bacterial proteins -- Properties, Biological sciences
Abstract

The surface of polyhydroxybutyrate (PHB) storage granules in bacteria is covered mainly by proteins referred to as phasins. The layer of phasins stabilizes the granules and prevents coalescence of separated granules in the cytoplasm and nonspecific binding of other proteins to the hydrophobic surfaces of the granules. Phasin Pha[P1.sub.Reu] is the major surface protein of PHB granules in Ralstonia eutropha H16 and occurs along with three homologues (PhaP2, PhaP3, and PhaP4) that have the capacity to bind to PHB granules but are present at minor levels. All four phasins lack a highly conserved domain but share homologous hydrophobic regions. To identify the region of [Pha[P1.sub.Reu] which is responsible for the binding of the protein to the granules, N-terminal and C-terminal fusions of enhanced green fluorescent protein with [Pha[P1.sub.Reu] or various regions of [Pha[P1.sub.Reu] were generated by recombinant techniques. The fusions were localized in the cells of various recombinant strains by fluorescence microscopy, and their presence in different subcellular protein fractions was determined by immunodetection of blotted proteins. The fusions were also analyzed to determine their capacities to bind to isolated PHB granules in vitro. The results of these studies indicated that unlike the phasin of Rhodococcus ruber, there is no discrete binding motif; instead, several regions of [Pha[P1.sub.Reu] contribute to the binding of this protein to the surface of the granules. The conclusions are supported by the results of a small-angle X-ray scattering analysis of purified [Pha[P1.sub.Reu], which revealed that [Pha[P1.sub.Reu] is a planar, triangular protein that occurs as trimer. This study provides new insights into the structure of the PHB granule surface, and the results should also have an impact on potential biotechnoiogical applications of phasin fusion proteins and PHB granules in nanobiotechnology.

Published
2008

11. Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements

Author

Sovkova Vera, Rustichelli Franco, Lukasova Vera, Doupník Miroslav, Staffa Andrea, Litvinec Andrej, Filova Eva, Voltrová Barbora, Tonar Zbyněk, Kralickova Milena, Kubíková Tereza, Blahnová Veronika, Amler Evzen, Rampichova Michala, Vocetková Karolína, Buzgo Matej, and Svora Petr

Subjects
0301 basic medicine, Scaffold, Chemistry, General Chemical Engineering, Bone implant, Mesenchymal stem cell, 02 engineering and technology, General Chemistry, 021001 nanoscience & nanotechnology, 03 medical and health sciences, 030104 developmental biology, In vivo, Drug delivery, Term delivery, 0210 nano-technology, Bone regeneration, Spinning, Biomedical engineering
Abstract

Bone regeneration is a long-term process requiring proper scaffolding and drug delivery systems. The current study delivers a three-dimensional (3D) scaffold prepared by blend centrifugal spinning loaded with the osteogenic supplements (OS) β-glycerol phosphate, ascorbate-2-phosphate and dexamethasone. The OS were successfully encapsulated into a fibrous scaffold and showed sustained release for 30 days. Furthermore, biological testing showed the osteoinductive properties of the scaffolds on a model of human mesenchymal stem cells and stimulatory effect on a model of osteoblasts. The osteoinductive properties were further proved in vivo in critical size defects of rabbits. The amount of bone trabecules was bigger compared to control fibers without OS. The results indicate that due to its long-term drug releasing properties, single step fabrication process and 3D structure, the system shows ideal properties for use as a cell-free bone implant in tissue-engineering.

Published
2018
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14. VCAM-1 expression on dystrophic muscle vessels has a critical role in the recruitment of human blood-derived CD133+ stem cells after intra-arterial transplantation

Author

Gavina, Manuela, Belicchi, Marzia, Rossi, Barbara, Ottoboni, Linda, Colombo, Fabio, Meregalli, Mirella, Battistelli, Maurizio, Forzenigo, Laura, Biondetti, Piero, Pisati, Federica, Parolini, Daniele, Farini, Andrea, Issekutz, Andrew C., Bresolin, Nereo, Rustichelli, Franco, Constantin, Gabriela, and Torrente, Yvan

Published
2006
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18. Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Author

Rampichová,Michala, Buzgo,Matej, Míčková,Andrea, Vocetková,Karolína, Sovková,Věra, Lukášová,Vera, Filová,Eva, Rustichelli,Franco, Amler,Evžen, Rampichová,Michala, Buzgo,Matej, Míčková,Andrea, Vocetková,Karolína, Sovková,Věra, Lukášová,Vera, Filová,Eva, Rustichelli,Franco, and Amler,Evžen

Abstract

Michala Rampichová,1,2 Matej Buzgo,1 Andrea Míčková,1,2 Karolína Vocetková,2 Vera Sovková,2 Vera Lukášová,2 Eva Filová,2 Franco Rustichelli,2 Evžen Amler1,2 1Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštehrad, 2Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic Abstract: Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess large, open pores. Centrifugal spinning is a simple method for producing 3D fibrous scaffolds with large and interconnected pores. However, the process of bone regeneration is rather complex and requires additional stimulation by active molecules. In the current study, we introduced a simple composite scaffold based on platelet adhesion to poly-ε-caprolactone 3D fibers. Platelets were used as a natural source of growth factors and cytokines active in the tissue repair process. By immobilization in the fibrous scaffolds, their bioavailability was prolonged. The biological evaluation of the proposed system in the MG-63 model showed improved metabolic activity, proliferation and alkaline phosphatase activity in comparison to nonfunctionalized fibrous scaffold. In addition, the response of cells was dose dependent with improved biocompatibility with increasing platelet concentration. The results demonstrated the suitability of the system for bone tissue. Keywords: centrifugal spinning, 3D scaffold, platelets, growth factors, cytokines, PCL

Published
2017

28. Novel insight into stem cell trafficking in dystrophic muscles

Author

Farini,Andrea, Villa, Manescu, Fiori, Giuliani, Razini, Sitzia, Del Fraro, Belicchi, Meregalli,Mirella, Rustichelli,Franco, Torrente,Yvan, Farini,Andrea, Villa, Manescu, Fiori, Giuliani, Razini, Sitzia, Del Fraro, Belicchi, Meregalli,Mirella, Rustichelli,Franco, and Torrente,Yvan

Abstract

Andrea Farini,1,* Chiara Villa,1,* Adrian Manescu,2 Fabrizio Fiori,2 Alessandra Giuliani,2 Paola Razini,1 Clementina Sitzia,1 Giulia Del Fraro,1 Marzia Belicchi,1 Mirella Meregalli,1 Franco Rustichelli,2 Yvan Torrente1 1Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico di Milano, Centro Dino Ferrari, Università di Milano, Milano, Italy; 2Università Politecnica delle Marche, Di.S.C.O. - Sezione di Biologia, Biochimica e Fisica Ancona, Italy*These authors contributed equally to this paperAbstract: Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications.Keywords: iron nanoparticles, micro-CT, CD133+ stem cells, dystrophic muscles

Published
2012

30. Three Years After Transplants in Human Mandibles, Histological and In-Line Holotomography Revealed That Stem Cells Regenerated a Compact Rather Than a Spongy Bone: Biological and Clinical Implications

Author

Giuliani, Alessandra, primary, Manescu, Adrian, additional, Langer, Max, additional, Rustichelli, Franco, additional, Desiderio, Vincenzo, additional, Paino, Francesca, additional, De Rosa, Alfredo, additional, Laino, Luigi, additional, d'Aquino, Riccardo, additional, Tirino, Virginia, additional, and Papaccio, Gianpaolo, additional

Published
2013
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31. Bone Turnover in Wild Type and Pleiotrophin-Transgenic Mice Housed for Three Months in the International Space Station (ISS)

Author

Tavella, Sara, primary, Ruggiu, Alessandra, additional, Giuliani, Alessandra, additional, Brun, Francesco, additional, Canciani, Barbara, additional, Manescu, Adrian, additional, Marozzi, Katia, additional, Cilli, Michele, additional, Costa, Delfina, additional, Liu, Yi, additional, Piccardi, Federica, additional, Tasso, Roberta, additional, Tromba, Giuliana, additional, Rustichelli, Franco, additional, and Cancedda, Ranieri, additional

Published
2012
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32. High-resolution X-ray microtomography for three-dimensional imaging of cardiac progenitor cell homing in infarcted rat hearts

Author

Giuliani, Alessandra, primary, Frati, Caterina, additional, Rossini, Alessandra, additional, Komlev, Vladimir S., additional, Lagrasta, Costanza, additional, Savi, Monia, additional, Cavalli, Stefano, additional, Gaetano, Carlo, additional, Quaini, Federico, additional, Manescu, Adrian, additional, and Rustichelli, Franco, additional

Published
2011
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33. Neutron and Synchrotron Non-Destructive Methods for the Characterisation of Materials for Different Applications

Author

ANCONA UNIV (ITALY), Rustichelli, Franco, ANCONA UNIV (ITALY), and Rustichelli, Franco

Abstract

Viewgraphs of presentation on neutron and synchrotron non-destructive methods for the characterization of materials for different applications., See also ADM001672. The original document contains color images. Presented at the NATO Advanced Research Workshop on Metallic Materials with High Structural Efficiency held in Kiev, Ukraine on 7-13 Sep 2003.

Published
2003

34. Stem Cell Tracking by Nanotechnologies

Author

Villa, Chiara, primary, Erratico, Silvia, additional, Razini, Paola, additional, Fiori, Fabrizio, additional, Rustichelli, Franco, additional, Torrente, Yvan, additional, and Belicchi, Marzia, additional

Published
2010
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36. Organization of Extracellular Matrix Fibers Within Polyglycolic Acid–Polylactic Acid Scaffolds Analyzed Using X-Ray Synchrotron-Radiation Phase-Contrast Micro Computed Tomography

Author

Albertini, Gianni, primary, Giuliani, Alessandra, additional, Komlev, Vladimir, additional, Moroncini, Francesca, additional, Pugnaloni, Armanda, additional, Pennesi, Giuseppina, additional, Belicchi, Marzia, additional, Rubini, Corrado, additional, Rustichelli, Franco, additional, Tasso, Roberta, additional, and Torrente, Yvan, additional

Published
2009
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44. Microstructural Analysis and Residual Stress Determination based on Scattering of Neutrons and X-ray Synchrotron Radiation.

Author

Skrzypek, Jacek J., Ganczarski, Artur W., Egner, Halina, and Rustichelli, Franco

Abstract

A detailed treatment of the subjects of this section can be found in Batterman and Cole [16], Brüuckel [26], Cantor and Schimmel [30], Cowley [52], Dianoux and Lander[55], Feigin and Svergun [74], Glatter and Kratky [88], Guinier and Fournet [90], Jackson [117], Jacrot [118], Landau and Lifshitz [140], Sears [200], Squires [214]. [ABSTRACT FROM AUTHOR]

Published
2008
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45. Developing and Implementing Selected Constitutive Models for Elasto-Plastic-Damage Materials.

Author

Skrzypek, Jacek J., Ganczarski, Artur W., Egner, Halina, and Rustichelli, Franco

Abstract

The general thermodynamically based theory of the constitutive and evolution equations of elastic-brittle damaged materials in a total stress-strain formulation, developed by Murakami and Kamiya [162], is the key for a further extension presented in the paper by Kuna-Ciska l and Skrzypek [208]. The Helmholtz free energy is a function of the elastic strain tensor εe, the second-rank damage tensor D, and another scalar damage variable β. The following Helmholtz free energy decomposition into the elastic and the damage terms is postulated as the state potential (2.20): 3.1$$ \rho \Psi (\varepsilon ^e ,D,\beta ) = \rho \Psi ^e (\varepsilon ^e ,D) + \rho \Psi ^d (\beta ) $$ [ABSTRACT FROM AUTHOR]

Published
2008
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47. Thermodynamics of Constitutive Modelling of Damaged Materials.

Author

Skrzypek, Jacek J., Ganczarski, Artur W., Egner, Halina, and Rustichelli, Franco

Abstract

For nonhomogeneous materials the heat balance equation reads: 2.1$$ div[q(x,t)] + \frac{\partial } {{\partial t}}\{ \rho (x,t)c[x,T(x,t)]T(x,t)\} - Q(x,t) = 0 $$ where T (x, t) is the absolute temperature field, c[x, T (x, t)] is the specific heat, q(x, t) is the heat flux vector, and Q(x, t) is the rate of internal heat source. The heat flux vector is given by the Fourier heat conduction law2.2$$ q(x,t) = - \lambda [x,T(x,t)] \cdot gradT(x,t) $$ where λ[x, T (x, t)] is the second-rank temperature-dependent tensor of thermal conductivity for a nonhomogeneous material. If internal heat sources are neglected, the steady state heat flux equation for time-independent, nonhomogeneous but isotropic material holds (Tanigawa [216, 217]): 2.3$$ \begin{gathered} \frac{\partial } {{\partial x}}\left[ {\lambda (x,y,z)\frac{{\partial T(x,y,z)}} {{\partial x}}} \right] + \frac{\partial } {{\partial y}}\left[ {\lambda (x,y,z)\frac{{\partial T(x,y,z)}} {{\partial y}}} \right] \hfill \\ + \frac{\partial } {{\partial z}}\left[ {\lambda (x,y,z)\frac{{\partial T(x,y,z)}} {{\partial z}}} \right] = 0 \hfill \\ \end{gathered} $$ [ABSTRACT FROM AUTHOR]

Published
2008
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48. Material Properties for High Temperature Applications.

Author

Skrzypek, Jacek J., Ganczarski, Artur W., Egner, Halina, and Rustichelli, Franco

Abstract

In many applications, especially in the space industry (e.g. jet engines and rockets) as well as electronic industry (e.g. superheat resistant materials for thermal protection, vehicle and personal body armour, electromagnetic sensors), structures or part of structures are exposed to high temperature, usually up to 2000K or even 3500K in some parts of rocket engines (Schulz et al. [202]), high temperature gradients, and/or cyclic temperature changes or impact loading. Conventionalmetallic materials, such as carbon steels or stainless steels: ASTM 321, ASTM 310, nickel- or aluminium-based alloys cannot resist such high temperatures (Odqvist [175]). The first method to improve the resistance of metallic structures against extreme temperature conditions consists in covering the structure (a substrate) with a ceramic layer (Thermal Barrier Coating — TBC), since ceramics are known for their high thermal resistance. For instance, in a metal-ceramic composite: Al-SiC the thermal conductivities ratio is approximately equal: λm/λc = 3.6, the thermal expansion coefficients ratio: αm/αc = 5, whereas the elastic moduli ratio: Em/Ec = 0.16 (Poterasu et al. [181]). In the case of Ni-Al2O3 composite the corresponding ratios are: λm/λc = 2.95, αm/αc = 1.51 and Em/Ec = 0.50 (Chen and Tong [44]). Hence, at the metal-ceramic interface, severe discontinuity of thermomechanical properties occurs, which results in high strain and stress mismatch at the interface; as a consequence, delamination or failure of the coating is rapidly observed. [ABSTRACT FROM AUTHOR]

Published
2008
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