1. Blood-brain barrier transport using a high-affinity, brain-selective VNAR (Variable Domain of New Antigen Receptor) antibody targeting transferrin receptor 1
- Author
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Mykhaylo Demydchuk, Pawel Stocki, Frank S. Walsh, Logan Db, Thei L, Torben Moos, Jaroslaw Szary, Leandra Northall, Rutkowski Jl, Aziz Gauhar, and Rasmussen Cl
- Subjects
chemistry.chemical_classification ,Phage display ,biology ,Transferrin receptor ,Pharmacology ,Blood–brain barrier ,Immunolabeling ,medicine.anatomical_structure ,chemistry ,Ectodomain ,Transferrin ,In vivo ,medicine ,biology.protein ,Antibody - Abstract
Transfer across the blood-brain barrier (BBB) remains a significant hurdle for the development of biopharmaceuticals with therapeutic effects within the central nervous system. We established a functional selection method to identify high-affinity single domain antibodies to the transferrin receptor 1 (TfR1) with efficient biotherapeutic delivery across the BBB.MethodsA synthetic phage display library based on the variable domain of new antigen receptor (VNAR) was used forin vitroselection against recombinant human TfR1 ectodomain (rh-TfR1-ECD) followed byin vivoselection in mouse for brain parenchyma penetrating antibodies. Phage formatted VNARs cross-reactive to recombinant human and mouse TfR1-ECD were fused to Fc domain of human IgG1 (hFc) and tested for TfR1-ECD binding by ELISA and surface plasmon resonance. The pharmacokinetics and biodistribution of VNAR-hFcs were studied in mice by ELISA and immunolabeling following intravenous (IV) injection and cardiac perfusion. Functional activity was measured by body temperature reduction following the IV injection of neurotensin fused to a TXB2-hFc (TXB2-hFc-NT).ResultsTXB2 was identified as a high-affinity, species cross-reactive VNAR antibody against TfR1-ECD, that does not to compete with transferrin or ferritin for receptor binding. IV dosing of TXB2-hFc at 25 nmol/kg (1.875 mg/kg) in mice resulted in rapid binding to brain capillaries with subsequent transport into the brain parenchyma and specific uptake into TfR1-positive neurons. Likewise, IV dosing of TXB2-hFc-NT at 25 nmol/kg resulted in a rapid and reversible pharmacological response as measured by body temperature reduction. TXB2-hFc did not elicit any acute adverse reactions, bind or deplete circulating reticulocytes or reduce BBB-expressed endogenous TfR1 in mice. There was no evidence of target-mediated clearance or accumulation in peripheral organs except lung.ConclusionsA species cross-reactive and brain-selective VNAR antibody to TfR1 was identified by a combination ofin vitroandin vivophage selection. As a high-affinity, bivalent Fc fusion protein, TXB2 rapidly crossed the BBB and exhibited a favorable pharmacokinetic and safety profile and can be readily adapted to carry a wide variety of biotherapeutics from blood to brain.
- Published
- 2019