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Linear resonant actuators (LRAs) are used extensively in consumer electronic devices such as cell phones and fitness trackers to provide haptic signals to users. This work presents a dynamic model of LRAs based, principally, on the electromagnetic aspects of the these devices. The model predicts that the natural frequency exhibited by an LRA can deviate significantly from that predicted by conventional spring-mass-damper dynamic models. Preliminary experimental results appear to support theoretical findings.

Novel strategies for initiation and continuation of buprenorphine are critical, especially during a pandemic when traditional opioid use disorder treatment pathways may be disrupted. We describe an innovative outpatient to inpatient reallocation initiative for extended-release buprenorphine (XR-BUP) designed to repurpose an expensive medication for use in hospitalized patients facing treatment barriers upon discharge and pilot the feasibility of XR-BUP use in the inpatient setting.We collaborated with our institution's inpatient pharmacy and a New Jersey Medicaid managed care organization to create an alternate pathway to make XR-BUP available to hospitalized patients insured by the same payor. In this process, XR-BUP doses were deidentified and transferred to the inpatient controlled substance inventory for administration to hospitalized patients at no charge by our Addiction Medicine Consult Service after a period of sublingual buprenorphine stabilization. Our reallocation pathway bypassed several existing XR-BUP regulatory barriers to allow for inpatient administration.To date, we have transferred approximately 85 XR-BUP 300 mg doses to the inpatient controlled substance inventory. This equates to a cost savings of nearly $145,000.Reallocation of XR-BUP from an outpatient to inpatient setting increased postdischarge buprenorphine treatment access while also reducing health care costs by repurposing an expensive medication that would otherwise go to waste. Use of reallocated XR-BUP in the inpatient setting may pave the way for addition of XR-BUP to the hospital's formulary to minimize treatment gaps after discharge.

Background: With the creation of photo-based plant identification applications (apps), the ability to attain basic identifications of plants in the field is seemingly available to anyone who has access to a smartphone. The use of such apps as an educational tool for students and as a major identification resource for some community science projects calls into question the accuracy of the identifications they provide. We created a study based on the context of local tree species in order to offer an informed response to students asking for guidance when choosing a tool for their support in classes. Methods: This study tested 6 mobile plant identification apps on a set of 440 photographs representing the leaves and bark of 55 tree species common to the state of New Jersey (USA). Results: Of the 6 apps tested, PictureThis was the most accurate, followed by iNaturalist, with PlantSnap failing to offer consistently accurate identifications. Overall, these apps are much more accurate in identifying leaf photos as compared to bark photos, and while these apps offer accurate identifications to the genus-level, there seems to be little accuracy in successfully identifying photos to the species-level. Conclusions: While these apps cannot replace traditional field identification, they can be used with high confidence as a tool to assist inexperienced or unsure arborists, foresters, or ecologists by helping to refine the pool of possible species for further identification.

Inherited retinal diseases (IRDs) are a diverse set of visual disorders that collectively represent a major cause of early-onset blindness. With the reduction in sequencing costs in recent years, whole-genome sequencing (WGS) is being used more frequently, particularly when targeted gene panels and whole-exome sequencing (WES) fail to detect pathogenic mutations in patients. In this study, we performed mutation screens using WGS for a cohort of 311 IRD patients whose mutations were undetermined. A total of nine putative pathogenic mutations in six IRD patients were identified, including six novel mutations. Among them, four were deep intronic mutations that affected mRNA splicing, while the other five affected protein-coding sequences. Our results suggested that the rate of resolution of unsolved cases via targeted gene panels and WES can be further enhanced with WGS; however, the overall improvement may be limited.

Accurate same-day sexually transmitted infection (STI) diagnostic testing is generally unavailable, leading to syndromic management with high rates of overtreatment and undertreatment. We analyzed the ease of integration of the Visby STI Panel into clinical practice, studied acceptance by patients and clinic personnel, and assessed the potential to inform accurate treatment decisions.In a cross-sectional single-visit study of 55 women aged 18 to 56 years, women self-collected vaginal swab samples that were analyzed using the Visby STI Panel for Chlamydia trachomatis, Neisseria gonorrhoeae (NG), and Trichomonas vaginalis (TV). Results were compared with standard-of-care clinic results from send-out laboratory polymerase chain reaction tests. Surveys assessed patient and device operator experiences with the Visby STI Panel and clinicians' perceived need for and acceptance of the device. Time parameters were measured to evaluate the impact on clinical workflow, and syndromic treatment decisions were compared with anticipated treatment based on the Visby STI Panel results.Patients strongly agreed that sample self-collection was easy, and operators reported the device easy to use. Clinicians valued the rapid return of results, and patients were comfortable waiting up to 30 minutes to receive them. In 13 of 15 cases, the Visby STI Panel correctly identified undertreated patients as infected and correctly identified all 33 incidences of overtreatment.Clinical adoption of the Visby STI Panel into primary care clinics and doctors' offices could reduce overtreatment and undertreatment of STIs. If integrated efficiently into the clinical workflow, the test would have minimal impact on staff time and visit duration for patients.

Trauma holds a sacred space in the knowledge bundles of many Indigenous helpers, including myself.A knowledge bundle represents the knowledge that an individual holds within themselves. This bundleconsists of experiential knowledge, knowledge shared through teachings, and knowledge received throughgenetic memory. This article explores the potential trauma Indigenous social workers may face priorto practice, within post-secondary programs, and during practice. The literature written by Indigenoushelpers working alongside trauma in the field of social work provides incredibly valuable knowledge forcurrent helpers, as well as for students just beginning their journey into the social work field. How mightIndigenous helpers working within the field of social work care for the trauma they may carry within theirown knowledge bundles? This article examines how Indigenous helpers prepare to work with trauma,how they may navigate trauma within practice, and what happens after re-stimulation of traumatic events.

Domesticated dogs have been present in the Iberian Peninsula long before other domesticated species, back to the late Palaeolithic period. Their origin is still uncertain, but dogs were already well established during the Chalcolithic period (ca. 5000–4000 BP). This study employed a multidisciplinary approach comprising osteometric, radiographic and palaeogenomic analyses to characterize Chalcolithic Iberian Canis remains. Two Chalcolithic archaeological sites – Leceia, Oeiras, in Portugal, and El Casetón de la Era, Villalba de los Alcores, Valladolid, in Spain – were the main focus of this study. Osteometric and odontometric data from eleven other sites in Iberia were also included. Osteometric results show signs of phenotypic variability, likely the result of human-driven selective pressure. Dental radiographic and dental wear analyses allowed age at death estimation for four individuals (two juvenile and two adults). Three Chalcolithic Iberian dogs had their mitogenomes resequenced and the mitochondrial DNA analysis allowed to assign each individual to two of the major known haplogroups – A and C. Molecular sex infered by the chromosomeX/chromosome1 coverage ratio allowed to identify one female and two males. This study unveils some aspects of the Iberian Chalcolithic dogs: these dogs already exhibited various morphotypes whose profiles might be associated to the performance of certain tasks, as well as mitogenomes of two distinct lineages that help tracking the evolutionary paths of Iberian dogs., The research leading to these results has received funding from FEDER Funds through the Operational Competitiveness Factors Program “COMPETE”, and by National Funds through the Foundation for Science and Technology (FCT Grant POCI-01-0145-FEDER-029545 – PTDC/HAR-ARQ/29545/2017), by the project UIDB/CVT/00772/2020 and associated laboratory AL4AnimalS, as well as from individual grants and contracts: DL57/2016/CP1440/CT0029 (A.E. Pires), DL57/2016/CP1375/CT0003 (I.R. Amorim), 2020.02754.CEECIND (C. Ginja), CEECINS/00127/2018 (J. Requicha), DL 57/2016/CP1443/CT0029 (C. Detry), 2020.06605.BD (A.B. Santos) and 2020.05503.BD (C. Delicado). A considerable amount of the osteometric data included in this research were obtained during the development of the Master thesis and PhD of A. Daza-Perea at Universidad Autónoma de Madrid, thanks to the granting of materials and expertise from C. Liesau, P. Ríos, Argea Consultores S.L., and Trébede, Patrimonio y Cultura S.L. We must thank the support of the FPI PhD BES-2012-056461 grant, which was associated with the HAR2011-28731 Project, both financed by the Spanish Ministry of Economy and Competitiveness.

Xylazine is an alpha-2 adrenergic agonist commonly used as a large animal anesthetic. It is used as an adulterant in illicit opioids, and it is now well established that its synergistic effect with opioids increases lethality. The amount of xylazine adulterating illicit opioids is growing at an alarming rate, present in almost one-third of opioid overdose deaths reported in Philadelphia in 2019. Despite this, there are no reports considering the management of patients using xylazine chronically. In particular, there are no reported cases detailing the management of xylazine withdrawal or exploring the potential for ongoing treatment for those in recovery from xylazine use.We present the case of a 29 year old female with opioid use disorder and chronic xylazine use, admitted to the intensive care unit for treatment of chronic lower extremity wounds thought to be due to xylazine injection. Her xylazine withdrawal was managed with a combination of dexmedetomidine infusion, phenobarbital and tizanidine, later transitioned to clonidine. By hospital day 4 she was no longer experiencing withdrawal symptoms. She was transitioned from full-agonist opioids for pain to buprenorphine via a buprenorphine "micro-induction" and was ultimately discharged on buprenorphine, clonidine, and gabapentin on day 19 of admission.This case illustrates a potential treatment pathway that allows for safe and comfortable xylazine withdrawal in hospitalized patients. It also provides an introduction into several medical concerns affecting this patient population specifically, including xylazine-mediated soft tissue wounds.

The transition to agriculture occurred relatively late in Eastern Europe, leading researchers to debate whether it was a gradual, interactive process or a colonization event. In the forest and forest-steppe regions of Ukraine, farming appeared during the fifth millennium BCE, associated with the Cucuteni-Trypillian Archaeological Complex (CTCC, 4800-3000 BCE). Across Europe, the Neolithization process was highly variable across space and over time. Here, we investigate the population dynamics of early agriculturalists from the eastern forest-steppe region based on analyses of 20 ancient genomes from the Verteba Cave site (3789-980 BCE). The results reveal that the CTCC individuals’ ancestry is related to both western hunter gatherers and Near Eastern farmers, lacks local ancestry associated with Ukrainian Neolithic hunter gatherers and has steppe ancestry. An Early Bronze Age individual has an ancestry profile related to the Yamnaya expansions but with 20% ancestry related to the other Trypillian individuals, which suggests admixture between the Trypillians and the incoming populations carrying steppe-related ancestry. A Late Bronze Age individual dated to 980-948 BCE has a genetic profile indicating affinity to Beaker-related populations, detected close to 1,000 years after the end of the Bell Beaker phenomenon during the Third millennium BCE.

Background: The addition of an addiction medicine consult service has been shown to improve mortality and decrease hospital costs but its impact on the proportion of patients discharged against medical advice (DAMA) and in-hospital initiation of medication for opioid use disorder (MOUD) has not been examined. Methods: A retrospective before-after cohort study was performed at an urban, academic medical center between January 1, 2015 and November 1, 2019. We included adult patients with infective endocarditis and injection drug use determined by admitting diagnosis ICD-9 or ICD-10 codes or documentation within the history section of electronic health recordEHR. Our institution implemented a formal addiction medicine consult service on July 1, 2018. We determined the proportion of patients DAMA and the proportion of patients started on MOUD among patients in the pre-intervention (i.e. hospitalized before July 1, 2018) and intervention (i.e. hospitalized July 1, 2018 or after) groups. Results: A total of 171 patients among hospitalized patients with injection drug use-associated infective endocarditis were included with 119 patients in the pre-intervention group and 52 patients in the intervention group. There was no statistically significant difference in patients DAMA [19% vs 15%, absolute risk difference 4.6% (95% confidence interval -8.6% to 17.7%)] between the intervention and pre-intervention groups. However, there was an increase in the proportion of inpatient MOUD initiation in the intervention group compared to the pre-intervention group [56% vs 21%, absolute risk difference 35% (95% confidence interval 19% to 50%)]. Conclusions: The initiation of an addiction medicine consult service was associated with a higher proportion of MOUD initiation but there was no statistically significant association with the proportion of patients DAMA.

Humans settled the Caribbean about 6,000 years ago, and ceramic use and intensified agriculture mark a shift from the Archaic to the Ceramic Age at around 2,500 years ago1,2,3. Here we report genome-wide data from 174 ancient individuals from The Bahamas, Haiti and the Dominican Republic (collectively, Hispaniola), Puerto Rico, Curaçao and Venezuela, which we co-analysed with 89 previously published ancient individuals. Stone-tool-using Caribbean people, who first entered the Caribbean during the Archaic Age, derive from a deeply divergent population that is closest to Central and northern South American individuals; contrary to previous work4, we find no support for ancestry contributed by a population related to North American individuals. Archaic-related lineages were >98% replaced by a genetically homogeneous ceramic-using population related to speakers of languages in the Arawak family from northeast South America; these people moved through the Lesser Antilles and into the Greater Antilles at least 1,700 years ago, introducing ancestry that is still present. Ancient Caribbean people avoided close kin unions despite limited mate pools that reflect small effective population sizes, which we estimate to be a minimum of 500–1,500 and a maximum of 1,530–8,150 individuals on the combined islands of Puerto Rico and Hispaniola in the dozens of generations before the individuals who we analysed lived. Census sizes are unlikely to be more than tenfold larger than effective population sizes, so previous pan-Caribbean estimates of hundreds of thousands of people are too large5,6. Confirming a small and interconnected Ceramic Age population7, we detect 19 pairs of cross-island cousins, close relatives buried around 75 km apart in Hispaniola and low genetic differentiation across islands. Genetic continuity across transitions in pottery styles reveals that cultural changes during the Ceramic Age were not driven by migration of genetically differentiated groups from the mainland, but instead reflected interactions within an interconnected Caribbean world1,8., This work was supported by a grant from the National Geographic Society to M. Pateman to facilitate analysis of skeletal material from The Bahamas and by a grant from the Italian ‘Ministry of Foreign Affairs and International Cooperation’ (Italian archaeological, anthropological and ethnological missions abroad, DGPSP Ufficio VI). D.R. was funded by NSF HOMINID grant BCS-1032255, NIH (NIGMS) grant GM100233, the Paul Allen Foundation, the John Templeton Foundation grant 61220 and the Howard Hughes Medical Institute.

Funder: The excavation of the Ikawazu Jomon individual was supported by Grant-in-Aid for Scientific Research (B) (25284157) to YY. The Ikawazu Jomon genome project was organized by HI, and TH & HO who were supported by MEXT KAKENHI Grant Numbers 16H06408 and 17H05132, by Grant-in-Aid for Scientific Research on Innovative Areas (Cultural History of Paleoasia), and by Grant-in-Aid for Challenging Exploratory Research (23657167) and for Scientific Research (B) (17H03738). The Ikawazu Jomon genome sequencing was supported by JSPS KAKENHI Grant Number 16H06279 to ATo, and partly supported in the CHOZEN project in Kanazawa University, and in the Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University. Computations for the Ikawazu Jomon genome were partially performed on the NIG supercomputer at ROIS National Institute of Genetics., Anatomically modern humans reached East Asia more than 40,000 years ago. However, key questions still remain unanswered with regard to the route(s) and the number of wave(s) in the dispersal into East Eurasia. Ancient genomes at the edge of the region may elucidate a more detailed picture of the peopling of East Eurasia. Here, we analyze the whole-genome sequence of a 2,500-year-old individual (IK002) from the main-island of Japan that is characterized with a typical Jomon culture. The phylogenetic analyses support multiple waves of migration, with IK002 forming a basal lineage to the East and Northeast Asian genomes examined, likely representing some of the earliest-wave migrants who went north from Southeast Asia to East Asia. Furthermore, IK002 shows strong genetic affinity with the indigenous Taiwan aborigines, which may support a coastal route of the Jomon-ancestry migration. This study highlights the power of ancient genomics to provide new insights into the complex history of human migration into East Eurasia.

Humans settled the Caribbean ~6,000 years ago, with intensified agriculture and ceramic use marking a shift from the Archaic Age to the Ceramic Age ~2,500 years ago. To shed new light on the history of Caribbean people, we report genome-wide data from 184 individuals predating European contact from The Bahamas, Cuba, Hispaniola, Puerto Rico, Curaçao, and northwestern Venezuela. A largely homogeneous ceramic-using population most likely originating in northeastern South America and related to present-day Arawak-speaking groups moved throughout the Caribbean at least 1,800 years ago, spreading ancestry that is still detected in parts of the region today. These people eventually almost entirely replaced Archaic-related lineages in Hispaniola but not in northwestern Cuba, where unadmixed Archaic-related ancestry persisted into the last millennium. We document high mobility and inter-island connectivity throughout the Ceramic Age as reflected in relatives buried ~75 kilometers apart in Hispaniola and low genetic differentiation across many Caribbean islands, albeit with subtle population structure distinguishing the Bahamian islands we studied from the rest of the Caribbean and from each other, and long-term population continuity in southeastern coastal Hispaniola differentiating this region from the rest of the island. Ceramic-associated people avoided close kin unions despite limited mate pools reflecting low effective population sizes (2Ne=1000-2000) even at sites on the large Caribbean islands. While census population sizes can be an order of magnitude larger than effective population sizes, pan-Caribbean population size estimates of hundreds of thousands are likely too large. Transitions in pottery styles show no evidence of being driven by waves of migration of new people from mainland South America; instead, they more likely reflect the spread of ideas and people within an interconnected Caribbean world.

Inside-outside determination is a basic building block for higher-level geometry processing operations. Generalized winding numbers provide a robust answer for triangle meshes, regardless of defects such as self-intersections, holes or degeneracies. In this paper, we further generalize the winding number to point clouds. Previous methods for evaluating the winding number are slow for completely disconnected surfaces, such as triangle soups or-in the extreme case- point clouds. We propose a tree-based algorithm to reduce the asymptotic complexity of generalized winding number computation, while closely approximating the exact value. Armed with a fast evaluation, we demonstrate the winding number in a variety of new applications: voxelization, signing distances, generating 3D printer paths, defect-tolerant mesh booleans and point set surfaces.

Excavations at several locations in Verteba Cave have uncovered a large amount of human skeletal remains in association with faunal bones and Tripolye material culture. We aim to establish radiocarbon (14C) dates for eight sites and to evaluate whether these deposits are singular events, or slow accumulations over time.14C measurements, along with stable carbon and nitrogen isotope data from human and faunal remains, were collected from 18 specimens. Stable isotope values were used to evaluate human and animal diet, and whether freshwater reservoir effects offset measured dates. We found diets of the sampled species had limited to no influence from freshwater resources. Human diet appears to be dominated by terrestrial plants and herbivores. Four new sites were identified as Eneolithic. Comparisons of dates from top and bottom strata for two sites (7 and 20) reveal coeval dates, and we suggest that these deposits represent discrete events rather than slow continuous use. Lastly, we identified dates from the Mesolithic (8490±45 BP, 8765±30 BP), Iron Age (2505±20 BP), Slavic state era (1315±25 BP), and Medieval Period (585±15 BP), demonstrating periodic use of the cave by humans prior to and after the Eneolithic.

Many researchers have pointed to the huge “megasites” and construction of fortifications as evidence of intergroup hostilities among the Late Neolithic Tripolye archaeological culture. However, to date, very few skeletal remains have been analyzed for the types of traumatic injury that serve as direct evidence for violent conflict. In this study, we examine trauma on human remains from the Tripolye site of Verteba Cave in western Ukraine. The remains of 36 individuals, including 25 crania, were buried in the gypsum cave as secondary interments. The frequency of cranial trauma is 30-44% among the 25 crania, six males, four females and one adult of indeterminate sex displayed cranial trauma. Of the 18 total fractures, 10 were significantly large and penetrating suggesting lethal force. Over half of the trauma is located on the posterior aspect of the crania, suggesting the victims were attacked from behind. Sixteen of the fractures observed were perimortem and two were antemortem. The distribution and characteristics of the fractures suggest that some of the Tripolye individuals buried at Verteba Cave were victims of a lethal surprise attack. Resources were limited due to population growth and migration, leading to conflict over resource access. It is hypothesized that during this time of change burial in this cave aided in development of identity and ownership of the local territory.

Frantz, Laurent/0000-0001-8030-3885; Ersmark, Erik/0000-0003-4186-7498; Sjogren, Karl-Goran/0000-0003-1791-3175; Bergstrom, Anders/0000-0002-4096-9268 WOS:000583031800042 PubMed ID: 33122379 Dogs were the first domestic animal, but little is known about their population history and to what extent it was linked to humans. We sequenced 27 ancient dog genomes and found that all dogs share a common ancestry distinct from present-day wolves, with limited gene flow from wolves since domestication but substantial dog-to-wolf gene flow. By 11,000 years ago, at least five major ancestry lineages had diversified, demonstrating a deep genetic history of dogs during the Paleolithic. Coanalysis with human genomes reveals aspects of dog population history that mirror humans, including Levant-related ancestry in Africa and early agricultural Europe. Other aspects differ, including the impacts of steppe pastoralist expansions in West and East Eurasia and a near-complete turnover of Neolithic European dog ancestry. SciLifeLab National Projects; Erik Philip Sorensen Foundation; Francis Crick Institute from Cancer Research UK [FC001595]; UK Medical Research CouncilMedical Research Council UK (MRC); Wellcome TrustWellcome Trust [210119/Z/18/Z]; European Research CouncilEuropean Research Council (ERC) [852558]; Wellcome Trust Investigator awardWellcome Trust [217223/Z/19/Z]; Vallee Foundation; Social Sciences and Humanities Research Council of CanadaSocial Sciences and Humanities Research Council of Canada (SSHRC) [SSHRC IG 435-2014-0075]; State Assignment of the Sobolev Institute of Geology and Mineralogy; ZIN RAS [AAA-A19119032590102-7]; Smithsonian's Peter Buck Postdoctoral Fellowship; AHRC [AH/J001406/1]; SNIC-UPPMAX [b2016004]; UOXF ARC facilityAustralian Research Council; Wolfson College (University of Oxford); ERCEuropean Research Council (ERC) [ERC-2013-StG-337574-UNDEAD]; Natural Environmental Research CouncilNERC Natural Environment Research Council [NE/K005243/1, NE/K003259/1]; NERC Radiocarbon Facility [NF/2016/2/4] Ancient genome sequencing was supported by SciLifeLab National Projects and the Erik Philip Sorensen Foundation (to P.S.). A.B., T.D., and P.S. were supported by the Francis Crick Institute core funding (FC001595) from Cancer Research UK, the UK Medical Research Council, and the Wellcome Trust. P.S. was also supported by the European Research Council (grant no. 852558), a Wellcome Trust Investigator award (217223/Z/19/Z) and the Vallee Foundation. R.J.L. was supported by the Social Sciences and Humanities Research Council of Canada (#SSHRC IG 435-2014-0075). Y.K. was supported by State Assignment of the Sobolev Institute of Geology and Mineralogy. M.S. was supported by ZIN RAS (state assignment no. AAA-A19119032590102-7). A.T.L. was supported by the Smithsonian's Peter Buck Postdoctoral Fellowship. Archaeological work in Serbia was supported by AHRC grant AH/J001406/1. Computations were supported by SNIC-UPPMAX (b2016004) and the UOXF ARC facility. L.F. was supported by the Wellcome Trust (grant 210119/Z/18/Z) and by Wolfson College (University of Oxford). G.L. was supported by the ERC (grant ERC-2013-StG-337574-UNDEAD). G.L. and K.D. were supported by the Natural Environmental Research Council (grants NE/K005243/1 and NE/K003259/1). Dating was supported by the NERC Radiocarbon Facility (NF/2016/2/4).

The acquisition of biological information and assessment of the most probable geographic origin of unidentified individuals for obtaining positive identification is central in forensic sciences. Identification based on forensic DNA, however, varies greatly in relation to degradation of DNA. Our primary aim is to assess the applicability of a petrous bone sampling method in combination with Next Generation Sequencing to evaluate the quality and quantity of DNA in taphonomically degraded petrous bones from forensic and cemetery cases. A related aim is to analyse the genomic data to obtain the molecular sex of each individual, and their most probable geographic origin. Six of seven subjects were previously identified and used for comparison with the results. To analyse their probable geographic origin, samples were genotyped for the 627.719 SNP positions. Results show that the inner ear cochlear region of the petrous bone provides good percentages of endogenous DNA (14.61–66.89%), even in the case of burnt bodies. All comparisons between forensic records and genetic results agree (sex) and are compatible (geographic origin). The application of the proposed methodology may be a powerful tool for use in forensic scenarios, ranging from missing persons to unidentified migrants who perish when crossing borders. Irish Research Council University of Vienna 2019-06-10 JG: PDF updated with published version (OA CC license)

Anatomical modern humans reached East Asia by >40,000 years ago (kya). However, key questions still remain elusive with regard to the route(s) and the number of wave(s) in the dispersal into East Eurasia. Ancient genomes at the edge of East Eurasia may shed light on the detail picture of peopling to East Eurasia. Here, we analyze the whole-genome sequence of a 2.5 kya individual (IK002) characterized with a typical Jomon culture that started in the Japanese archipelago >16 kya. The phylogenetic analyses support multiple waves of migration, with IK002 forming a lineage basal to the rest of the ancient/present-day East Eurasians examined, likely to represent some of the earliest-wave migrants who went north toward East Asia from Southeast Asia. Furthermore, IK002 has the extra genetic affinity with the indigenous Taiwan aborigines, which may support a coastal route of the Jomon-ancestry migration from Southeast Asia to the Japanese archipelago. This study highlight the power of ancient genomics with the isolated population to provide new insights into complex history in East Eurasia.

This paper presents an interactive design interface for three-dimensional free-form musical wind instruments. The sound of a wind instrument is governed by the acoustic resonance as a result of complicated interactions of sound waves and internal geometries of the instrument. Thus, creating an original free-form wind instrument by manual methods is a challenging problem. Our interface provides interactive sound simulation feedback as the user edits, allowing exploration of original wind instrument designs. Sound simulation of a 3D wind musical instrument is known to be computationally expensive. To overcome this problem, we first model the wind instruments as a passive resonator, where we ignore coupled oscillation excitation from the mouthpiece. Then we present a novel efficient method to estimate the resonance frequency based on the boundary element method by formulating the resonance problem as a minimum eigenvalue problem. Furthermore, we can efficiently compute an approximate resonance frequency using a new technique based on a generalized eigenvalue problem. The designs can be fabricated using a 3D printer, thus we call the results "print-wind instruments" in association with woodwind instruments. We demonstrate our approach with examples of unconventional shapes performing familiar songs.

This paper examines Iron Age Mongolia during a time when nomadic tribes created the world's first steppe empire in Inner Asia. These aggregated tribes, known as Xiongnu (3rd century BC to the 2nd century AD), came to define steppe polity construction, later used by the Mongol Empire under the reign of Genghis Khan. They moved extensively over the eastern steppe and interacted, both in trade and intermarriage, with peoples from southern Siberia to Xinjiang. However, the Xiongnu as a people are relatively unknown to scholars, as they did not possess a written language. This study assesses Xiongnu population history and biological structure by analyzing craniofacial diversity via geometric morphometrics. Twenty-four coordinate cranial landmarks were used to test relationships among groups in the region and infer potential biological origins. The Relethford–Blangero R-matrix method was used to test hypotheses of phenotypic variation resulting from microevolutionary processes. This study hypothesizes biological continuity among Xiongnu individuals extending into modern Mongolian populations. Alternatively, long-range gene flow from adjacent geographic regions might suggest a complex population structure among the Xiongnu indicative of multiple populations controlling administrative functions. Results indicate the Xiongnu were potentially composed of at least two biologically distinct groups. Individuals from the elite cemetery of Borkhan Tolgoi (Egiin Gol) share their ancestry with a Bronze Age population from western Mongolia, and possibly, to a later migration of Turks, who came to rule the eastern steppe from the 6th to 8th centuries AD. The Xiongnu also evidence biological similarity with nomads from the Mongol Empire during the medieval period and modern Mongolians, as well as modern and ancient Central Asian, Chinese, and Siberian groups. These results are similar to ancient DNA studies that suggest a mix of Eastern and Western Eurasian haplogroups in the Xiongnu while also achieving consensus with models of steppe polity formation proposed by archaeologists who suggest local ties to extra-local groups through interactive exchange networks.

A 10,000-year transect of Roman populations Rome wasn't built (or settled) in a day. Antonio et al. performed an ancestral DNA analysis to investigate the genetic changes that occurred in Rome and central Italy from the Mesolithic into modern times. By examining 127 Roman genomes and their archaeological context, the authors demonstrate a major ancestry shift in the Neolithic between hunter gatherers and farmers. A second ancestry shift is observed in the Bronze Age, likely coinciding with trade and an increased movement of populations. Genetic changes track the historical changes occurring in Rome and reflect gene flow from across the Mediterranean, Europe, and North Africa over time. Science , this issue p. 708

THE PROBLEM A common cause of fraud and abuse in the medical system is the assignment of services with incorrect Current Procedural Terminology (CPT) codes, leading to billing for services that were not performed or misrepresentation of the actual services that were provided [1-3]. Additionally, incorrect CPT coding has been shown to result in “undercoding” of the service provided, ultimately resulting in a loss of potential revenue [3,4]. Only a few studies have evaluated the accuracy of CPT coding for imaging-related interventions, demonstrating that CPT coding errors are commonplace [3,5]. Application of correct CPT codes is essential for physicians, regardless of experience or the coding arrangements within their practices. A general review of our coding compliance found a surprising number of monthly coding errors involving our musculoskeletal interventional service. As a section, we believed that it was important to improve our coding accuracy and chose to initiate a project with the goal of decreasing our CPT coding errors. Additionally, because the ultimate responsibility for coding accuracy rests on the radiologist, this initiative also further educated our

3D printing technologies allow for more diverse shapes than are possible with molds and the cost of making just one single object is negligible compared to traditional production methods. However, not all shapes are suitable for 3D print. One of the remaining costs is therefore human time spent on analyzing and editing a shape in order to ensure that it is fit for production. In this paper, we seek to automate one of these analysis and editing tasks, namely improving the balance of a model to ensure that it stands. The presented method is based on solving an optimization problem. This problem is solved by creating cavities of air and distributing dense materials inside the model. Consequently, the surface is not deformed. However, printing materials with significantly different densities is often not possible and adding cavities of air is often not enough to make the model balance. Consequently, in these cases, we will apply a rotation of the object which only deforms the shape a little near the base. No user input is required but it is possible to specify manufacturing constraints related to specific 3D print technologies. Several models have successfully been balanced and printed using both polyjet and fused deposition modeling printers. We revisit a number of 3D print technologies and discuss their characteristics.We present an automatic, optimization based method for balancing 3D models.The balance is improved by creating internal cavities and by rotating the model.We pay special attention to make FDM printed models stand.

BackgroundThe Eneolithic (~ 5,500 yrBP) site of Verteba Cave in Western Ukraine contains the largest collection of human skeletal remains associated with the archaeological Cucuteni-Tripolye Culture. Their subsistence economy is based largely on agro-pastoralism and had some of the largest and most dense settlement sites during the Middle Neolithic in all of Europe. To help understand the evolutionary history of the Tripolye people, we performed mtDNA analyses on ancient human remains excavated from several chambers within the cave.ResultsBurials at Verteba Cave are largely commingled and secondary in nature. A total of 68 individual bone specimens were analyzed. Most of these specimens were found in association with well-defined Tripolye artifacts. We determined 28 mtDNA D-Loop (368 bp) sequences and defined 8 sequence types, belonging to haplogroups H, HV, W, K, and T. These results do not suggest continuity with local pre-Eneolithic peoples, but rather complete population replacement. We constructed maximum parsimonious networks from the data and generated population genetic statistics. Nucleotide diversity (π) is low among all sequence types and our network analysis indicates highly similar mtDNA sequence types for samples in chamber G3. Using different sample sizes due to the uncertainly in number of individuals (11, 28, or 15), we found Tajima’s D statistic to vary. When all sequence types are included (11 or 28), we do not find a trend for demographic expansion (negative but not significantly different from zero); however, when only samples from Site 7 (peak occupation) are included, we find a significantly negative value, indicative of demographic expansion.ConclusionsOur results suggest individuals buried at Verteba Cave had overall low mtDNA diversity, most likely due to increased conflict among sedentary farmers and nomadic pastoralists to the East and North. Early Farmers tend to show demographic expansion. We find different signatures of demographic expansion for the Tripolye people that may be caused by existing population structure or the spatiotemporal nature of ancient data. Regardless, peoples of the Tripolye Culture are more closely related to early European farmers and lack genetic continuity with Mesolithic hunter-gatherers or pre-Eneolithic groups in Ukraine.

The SurfCuit system integrates circuits into 3D prints by mounting them on the printed surface. SurfCuit does not require tedious circuit casing design or expensive setups, allowing users to build complex, highly conductive circuit patterns for consumer-level desktop fused decomposition modeling (FDM) 3D printers and thus expediting the process of circuit construction for 3D models.https://extras.computer.org/extra/mcg2017030052s1.mp4.

Interactive, smart objects-customized to individuals and uses-are central to many movements, such as tangibles, the internet of things (IoT), and ubiquitous computing. Yet, rapid prototyping both the form and function of these custom objects can be problematic, particularly for those with limited electronics or programming experience. Designers often need to embed custom circuitry; program its workings; and create a form factor that not only reflects the desired user experience but can also house the required circuitry and electronics. To mitigate this, we created Pineal, a design tool that lets end-users: (1) modify 3D models to include a smart watch or phone as its heart; (2) specify high-level interactive behaviours through visual programming; and (3) have the phone or watch act out such behaviours as the objects' "smarts". Furthermore, a series of prototypes show how Pineal exploits mobile sensing and output, and automatically generates 3D printed form-factors for rich, interactive, objects.

This paper introduces novel interactive techniques for designing original hand-launched free-flight glider airplanes which can actually fly. The aerodynamic properties of a glider aircraft depend on their shape, imposing significant design constraints. We present a compact and efficient representation of glider aerodynamics that can be fit to real-world conditions using a data-driven method. To do so, we acquire a sample set of glider flight trajectories using a video camera and the system learns a nonlinear relationship between forces on the wing and wing shape. Our acquisition system is much simpler to construct than a wind tunnel, but using it we can efficiently discover a wing model for simple gliding aircraft. Our resulting model can handle general free-form wing shapes and yet agrees sufficiently well with the acquired airplane flight trajectories. Based on this compact aerodynamics model, we present a design tool in which the wing configuration created by a user is interactively optimized to maximize flight-ability. To demonstrate the effectiveness of our tool for glider design by novice users, we compare it with a traditional design workflow.

This study quantifies the population history of Iron Age nomads of southern Siberia by analyzing craniofacial diversity among contemporaneous Bronze and Iron Age (7th–2nd centuries BC) groups and compares them to a larger geographic sample of modern Siberian and Central Asian populations. In our analyses, we focus on peoples of the Tagar and Pazyryk cultures, and Iron Age peoples of the Tuva region. Twenty­six cranial landmarks of the vault and facial skeleton were analyzed on a total of 461 ancient and modern individuals using geometric morphometric techniques. Male and female crania were separated to assess potential sex­biased migration patterns. We explore southern Siberian population history by including Turkic­speaking peoples, a Xiongnu Iron Age sample from Mongolia, and a Bronze Age sample from Xinjiang. Results show that male Pazyryk cluster closer to Iron Age Tuvans, while Pazyryk females are more isolated. Conversely, Tagar males seem more isolated, while Tagar females cluster amongst an Early Iron Age southern Siberian sample. When additional modern Siberian samples are included, Tagar and Pazyryk males cluster more closely with each other than females, suggesting possible sex­biased migration amongst different Siberian groups. This is evident in modern female Tuva, who cluster with modern female Kalmyk, while modern Tuvan males do not. Male and female Iron Age Tuvans are not closely related to modern Tuvan peoples living in the region today, resulting from the influx of the Xiongnu beginning in the Late Iron Age. Both male and female Pazyryk and Tagar crania appear more similar to Central Asia groups, especially the Kazakh and Uzbek samples. However, there is evidence that Tagar females have a common origin with the Yakut, a modern nomadic population that resides in northeastern Siberia. These results would suggest variable genetic contributions for both sexes from Central and East Asia.

We present ChronoFab, a 3D modeling tool to craft motion sculptures, tangible representations of 3D animated models, visualizing an object's motion with static, transient, ephemeral visuals that are left behind. Our tool casts 3D modeling as a dynamic art-form by employing 3D animation and dynamic simulation for the modeling of motion sculptures. Our work is inspired by the rich history of stylized motion depiction techniques in existing 3D motion sculptures and 2D comic art. Based on a survey of such techniques, we present an interface that enables users to rapidly explore and craft a variety of static 3D motion depiction techniques, including motion lines, multiple stroboscopic stamps, sweeps and particle systems, using a 3D animated object as input. In a set of professional and non-professional usage sessions, ChronoFab was found to be a superior tool for the authoring of motion sculptures, compared to traditional 3D modeling workflows, reducing task completion times by 79%.

Consider an algorithm for generating a triangle mesh interpolating a fixed set of 3D point samples, where the generated triangle set varies depending on some underlying parameters. In this paper we treat such an algorithm as a means of sampling the space of possible interpolant meshes, and then define a more robust algorithm based on drawing multiple such samples from this process and averaging them. As mesh connectivity graphs cannot be trivially averaged, we compute triangle statistics and then attempt to find a set of compatible triangles which maximize agreement between the sample meshes while also forming a manifold mesh. Essentially, each sample mesh ''votes'' for triangles, and hence we call our result a consensus mesh. Finding the optimal consensus mesh is combinatorially intractable, so we present an efficient greedy algorithm. We apply this strategy to two mesh generation processes-ball pivoting and localized tangent-space Delaunay triangulations. We then demonstrate that consensus meshing enables a generic decomposition of the meshing problem which supports trivial parallelization.

Forensic anthropology has long established the use of sex estimation and assessment of geographical origin in the identification of unidentified subjects, including victims of human rights atrocities and migrant diasporas. This research explores and develops innovative anthropological methods for the application of the recent advances in geometric morphometry and ancient DNA (aDNA) to the above post-mortem approaches of identification. The aim of this research is to develop novel standards for diagnosing sex and geographic origins useful for the identification of unknown human remains. Phase I of the project involves the use of 3D digitization and geometric morphometric approaches to increase the accuracy in sub-adult sex estimation and to analyze size, shape and degree of maturation of skeletal elements to detect patterns of similarities and differences between sexes. A representative sample of a population of known origin was generated using contemporary human skeletal remains of Italian sub-adults who died during the 1990s. Bone surfaces were digitized using a 3D laser scanner and 3D landmarks and semi-landmarks were developed to capture the variation of skeletal morphology. GPA and PCA will be used as multivariate analyses. Phase II focuses on geographic origin and uses 20 modern samples from different geographic origins and taphonomic contexts. An aDNA protocol was used to maximize the results in degraded modern bones. DNA extraction, library and sequenced using a Shotgun-sequencing approach were carried out in genomics laboratories at UCD. The geographic information will be predicted by comparing the results obtained by means of SNP genotypes with databases of known populations. This approach enables the attribution of the samples to different regions and creates a basis for personal identification.

In this article we derive an equation for the velocity of an arbitrary time-evolving implicit surface. Strictly speaking, only the normal component of the velocity is unambiguously defined. This is because an implicit surface does not have a unique parametrization. However, by enforcing a constraint on the evolution of the normal field we obtain a unique tangential component. We apply our formulas to surface tracking and to the problem of computing velocity vectors of a motion blurred blobby surface. Other possible applications are mentioned at the end of the article.