1. Increased resistance against tellurite is conferred by a mutation in the promoter region of uncommon tellurite resistance gene tehB in the ter-negative Shiga toxin-producing Escherichia coli O157:H7.
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Yuko Matsumoto, Kenichi Lee, Ryuya Akasaka, Hayato Honjo, Mitsumasa Koizumi, Toshio Sato, Akiko Kubomura, Nozomi Ishijima, Yukihiro Akeda, Makoto Ohnishi, and Sunao Iyoda
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ESCHERICHIA coli O157:H7 , *PROMOTERS (Genetics) , *GENE expression , *ESCHERICHIA coli , *WHOLE genome sequencing , *TOXINS - Abstract
Resistance to potassium tellurite (PT) is an important indicator in isolating Shiga toxin-producing Escherichia coli (STEC) O157:H7 and other major STEC serogroups. Common resistance determinant genes are encoded in the ter gene cluster. We found an O157:H7 isolate that does not harbor ter but is resistant to PT. One nonsynonymous mutation was found in another PT resistance gene, tehA, through whole-genome sequence analyses. To elucidate the contribution of this mutation to PT resistance, complementation of tehA and the related gene tehB in isogenic strains and quantitative RT-PCR were performed. The results indicated that the point mutation not only changed an amino acid of tehA, but also was positioned on a putative internal promoter of tehB and increased PT resistance by elevating tehB mRNA expression. Meanwhile, the amino acid change in tehA had negligible impact on the PT resistance. Comprehensive screening revealed that 2.3% of O157:H7 isolates in Japan did not harbor the ter gene cluster, but the same mutation in tehA was not found. These results suggested that PT resistance in E. coli can be enhanced through one mutational event even in ter-negative strains. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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