28 results on '"Sánchez-Arreguin, A."'
Search Results
2. The Developmental History of Ustilago maydis: A Saprophytic Yeast, a Mycelial Fungus, Mushroom-Like, and a Smut
- Author
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Ruiz-Herrera, José, Cabrera-Ponce, José L., León-Ramírez, Claudia, Pérez-Rodríguez, Fernando, Salazar-Chávez, Mayela, Sánchez-Arreguín, Alejandro, Vélez-Haro, John, Satyanarayana, Tulasi, editor, Deshmukh, Sunil Kumar, editor, and Deshpande, Mukund V., editor
- Published
- 2019
- Full Text
- View/download PDF
3. Analysis of the photoreceptors involved in the light-depending basidiocarp formation in Ustilago maydis
- Author
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Sánchez-Arreguin, José Alejandro, Cabrera-Ponce, José Luis, León-Ramírez, Claudia Geraldine, Camargo-Escalante, Martín Orlando, and Ruiz-Herrera, José
- Published
- 2020
- Full Text
- View/download PDF
4. Chitosan Is Necessary for the Structure of the Cell Wall, and Full Virulence of Ustilago maydis
- Author
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José Alejandro Sánchez-Arreguin, M. Lucila Ortiz-Castellanos, Angélica Mariana Robledo-Briones, Claudia Geraldine León-Ramírez, Domingo Martínez-Soto, and José Ruiz-Herrera
- Subjects
Ustilago maydis ,chitosan ,cell wall ,filamentation ,virulence ,Biology (General) ,QH301-705.5 - Abstract
Smut fungi comprise a large group of biotrophic phytopathogens infecting important crops, such as wheat and corn. U. maydis is a plant pathogenic fungus responsible for common smut in maize and teocintle. Through our analysis of the transcriptome of the yeast-to-mycelium dimorphic transition at acid pH, we determined the number of genes encoding chitin deacetylases of the fungus, and observed that the gene encoding one of them (UMAG_11922; CDA1) was the only one up-regulated. The mutation of this gene and the analysis of the mutants revealed that they contained reduced amounts of chitosan, were severely affected in their virulence, and showed aberrant mycelial morphology when grown at acid pH. When the CDA1 gene was reinserted into the mutants by the use of an autonomous replication plasmid, virulence and chitosan levels were recovered in the retro mutant strains, indicating that the CDA1 gene was involved in these features. These data revealed that chitosan plays a crucial role in the structure and morphogenesis of the cell wall during mycelial development of the fungus, and that in its absence, the cell wall becomes altered and is unable to support the stress imposed by the defense mechanism mounted on by the plant host during the infection process.
- Published
- 2022
- Full Text
- View/download PDF
5. Acid pH Strategy Adaptation through NRG1 in Ustilago maydis
- Author
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José Alejandro Sánchez-Arreguin, José Ruiz-Herrera, F. de Jesus Mares-Rodriguez, Claudia Geraldine León-Ramírez, Lino Sánchez-Segura, Patricio Adrián Zapata-Morín, Jordan Coronado-Gallegos, and Elva Teresa Aréchiga-Carvajal
- Subjects
Ustilago maydis ,NRG1 ,pH ,transcript regulation ,hyphae ,filamentation ,Biology (General) ,QH301-705.5 - Abstract
The role of the Ustilago maydis putative homolog of the transcriptional repressor ScNRG1, previously described in Saccharomyces cerevisiae, Candida albicans and Cryptococcus neoformans, was analyzed by means of its mutation. In S. cerevisiae this gene regulates a set of stress-responsive genes, and in C. neoformans it is involved in pathogenesis. It was observed that the U. maydisNRG1 gene regulates several aspects of the cell response to acid pH, such as the production of mannosyl-erythritol lipids, inhibition of the expression of the siderophore cluster genes, filamentous growth, virulence and oxidative stress. A comparison of the gene expression pattern of the wild type strain versus the nrg1 mutant strain of the fungus, through RNA Seq analyses, showed that this transcriptional factor alters the expression of 368 genes when growing at acid pH (205 up-regulated, 163 down-regulated). The most relevant genes affected by NRG1 were those previously reported as the key ones for particular cellular stress responses, such as HOG1 for osmotic stress and RIM101 for alkaline pH. Four of the seven genes included WCO1 codifying PAS domain ( These has been shown as the key structural motif involved in protein-protein interactions of the circadian clock, and it is also a common motif found in signaling proteins, where it functions as a signaling sensor) domains sensors of blue light, two of the three previously reported to encode opsins, one vacuolar and non-pH-responsive, and another one whose role in the acid pH response was already known. It appears that all these light-reactive cell components are possibly involved in membrane potential equilibrium and as virulence sensors. Among previously described specific functions of this transcriptional regulator, it was found to be involved in glucose repression, metabolic adaptation to adverse conditions, cellular transport, cell rescue, defense and interaction with an acidic pH environment.
- Published
- 2021
- Full Text
- View/download PDF
6. Tec1, a member of the TEA transcription factors family, is involved in virulence and basidiocarp development in Ustilago maydis
- Author
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José Luis Cabrera-Ponce, Lino Sánchez-Segura, M Lucila Ortiz-Castellanos, José Alejandro Sánchez-Arreguin, Claudia G. León-Ramírez, José Ruiz-Herrera, Elva T. Aréchiga-Carvajal, Domingo Martínez-Soto, and Mayela Fernanda Salazar-Chávez
- Subjects
Microbiology (medical) ,Genetics ,0303 health sciences ,Virulence ,Ascomycota ,030306 microbiology ,Ustilago ,Basidiomycota ,Mutant ,Biology ,biology.organism_classification ,Microbiology ,Fungal Proteins ,03 medical and health sciences ,Basidiocarp ,Fruiting Bodies, Fungal ,Transcription factor ,Gene ,Transcription Factors ,030304 developmental biology ,Dikaryon - Abstract
The life cycle of Ustilago maydis involves alternation of a haploid saprophytic yeast-like stage and a dikaryotic hyphal virulent form. Under in vitro conditions, basidiocarps are formed. Analysis of the transcriptional network of basidiocarp formation revealed the possible involvement of a Tec transcription factor (Tec1, UMAG_02835) in the process. In some Ascomycota, Tec factors are involved in mycelial formation, pathogenesis, and interaction with other regulatory elements, but their role in Basidiomycota species is almost unknown. Accordingly, we proceeded to determine the role of this gene in U. maydis by its mutation. Tec1 was found to be a crucial factor for normal mating, basidiocarp development, and virulence, all of the functions related to the dikaryotic stage dependent of the b genes, whereas dimorphism and resistance to different stress conditions occurring in the haploid stage were not affected in tec1 mutants. The observation that mutants showed a low residual wild-type phenotype suggests the presence of a secondary mechanism that partially compensates the loss of Tec1.
- Published
- 2021
7. Chitosan Is Necessary for the Structure of the Cell Wall, and Full Virulence of Ustilago maydis
- Author
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Sánchez-Arreguin, José Alejandro, primary, Ortiz-Castellanos, M. Lucila, additional, Robledo-Briones, Angélica Mariana, additional, León-Ramírez, Claudia Geraldine, additional, Martínez-Soto, Domingo, additional, and Ruiz-Herrera, José, additional
- Published
- 2022
- Full Text
- View/download PDF
8. Generation of BSA-capsaicin Nanoparticles and Their Hormesis Effect on the Rhodotorula mucilaginosa Yeast
- Author
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Alejandro Sánchez-Arreguin, Ramón Carriles, Neftalí Ochoa-Alejo, Mercedes G. López, and Lino Sánchez-Segura
- Subjects
capsaicin ,bovine serum albumin ,nanoparticles ,fungi ,Rhodoturola ,hormesis ,Organic chemistry ,QD241-441 - Abstract
Capsaicin is a chemical compound found in pungent chili peppers (Capsicum spp.). In biotechnology, capsaicin has been proposed as a pathogen control; however, its low solubility in water and high instability limits its uses. The aim of this work was to study the effect of high concentrations of capsaicin on the synthesis of nanoparticles and to evaluate their inhibitory effect on the growth of Rhodotorula mucilaginosa yeast. Bovine serum albumin (BSA)-capsaicin nanoparticles were formulated at 0, 16.2, 32.5, 48.7 and 65.0 µg of capsaicin per mg of BSA. Nanoparticle properties were evaluated and they were added to cultures of R. mucilaginosa to quantify their effect on cell viability. We found that increased capsaicin levels caused several changes to the physicochemical parameters, probably due to changes in the hydrophobicity sites of the albumin during the nanostructuration. The administration of nanoparticles to cultures of R. mucilaginosa produced a maximal viability with nanoparticles at 16.2 µg/mg; on the contrary, nanoparticles at 65.0 µg/mg caused maximal cell death. R. mucilaginosa cells displayed a hormesis effect in response to the nanoparticle dose concentration. The nanoparticles showed different responses during the uptake process, probably as a consequence of the nanostructural properties of capsaicin in the BSA molecules.
- Published
- 2019
- Full Text
- View/download PDF
9. Alumbrado Exterior Fotovoltaico en la Universidad Tecnológica de Puebla.
- Author
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Cervantes De La Rosa, Juan Pedro, Muñoz Mata, José Lorenzo, Rojas Garnica, Juan Carlos, Bonilla Trejo, Juan Carlos, and Sánchez Arreguin, Judith
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
10. Tec1, a member of the TEA transcription factors family, is involved in virulence and basidiocarp development in Ustilago maydis
- Author
-
León-Ramírez, Claudia Geraldine, primary, Sánchez-Arreguin, José Alejandro, additional, Cabrera-Ponce, José Luis, additional, Martínez-Soto, Domingo, additional, Ortiz-Castellanos, M. Lucila, additional, Aréchiga-Carvajal, Elva Teresa, additional, Salazar-Chávez, Mayela Fernanda, additional, Sánchez-Segura, Lino, additional, and Ruiz-Herrera, José, additional
- Published
- 2021
- Full Text
- View/download PDF
11. Acid pH Strategy Adaptation through NRG1 in Ustilago maydis
- Author
-
Elva T. Aréchiga-Carvajal, F. de Jesus Mares-Rodriguez, Claudia G. León-Ramírez, José Alejandro Sánchez-Arreguin, José Ruiz-Herrera, Lino Sánchez-Segura, Jordan Coronado-Gallegos, and Patricio A. Zapata-Morin
- Subjects
Microbiology (medical) ,Ustilago ,Saccharomyces cerevisiae ,Ustilago maydis ,Plant Science ,medicine.disease_cause ,Article ,03 medical and health sciences ,PAS domain ,Gene expression ,medicine ,Transcriptional regulation ,hyphae ,transcript regulation ,Structural motif ,Gene ,lcsh:QH301-705.5 ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,0303 health sciences ,Mutation ,NRG1 ,biology ,030306 microbiology ,Chemistry ,pH ,biology.organism_classification ,Cell biology ,filamentation ,lcsh:Biology (General) - Abstract
The role of the Ustilago maydis putative homolog of the transcriptional repressor ScNRG1, previously described in Saccharomyces cerevisiae, Candida albicans and Cryptococcus neoformans, was analyzed by means of its mutation. In S. cerevisiae this gene regulates a set of stress-responsive genes, and in C. neoformans it is involved in pathogenesis. It was observed that the U. maydisNRG1 gene regulates several aspects of the cell response to acid pH, such as the production of mannosyl-erythritol lipids, inhibition of the expression of the siderophore cluster genes, filamentous growth, virulence and oxidative stress. A comparison of the gene expression pattern of the wild type strain versus the nrg1 mutant strain of the fungus, through RNA Seq analyses, showed that this transcriptional factor alters the expression of 368 genes when growing at acid pH (205 up-regulated, 163 down-regulated). The most relevant genes affected by NRG1 were those previously reported as the key ones for particular cellular stress responses, such as HOG1 for osmotic stress and RIM101 for alkaline pH. Four of the seven genes included WCO1 codifying PAS domain ( These has been shown as the key structural motif involved in protein-protein interactions of the circadian clock, and it is also a common motif found in signaling proteins, where it functions as a signaling sensor) domains sensors of blue light, two of the three previously reported to encode opsins, one vacuolar and non-pH-responsive, and another one whose role in the acid pH response was already known. It appears that all these light-reactive cell components are possibly involved in membrane potential equilibrium and as virulence sensors. Among previously described specific functions of this transcriptional regulator, it was found to be involved in glucose repression, metabolic adaptation to adverse conditions, cellular transport, cell rescue, defense and interaction with an acidic pH environment.
- Published
- 2021
12. Acid pH Strategy Adaptation through NRG1 in Ustilago maydis
- Author
-
Sánchez-Arreguin, José Alejandro, primary, Ruiz-Herrera, José, additional, Mares-Rodriguez, F. de Jesus, additional, León-Ramírez, Claudia Geraldine, additional, Sánchez-Segura, Lino, additional, Zapata-Morín, Patricio Adrián, additional, Coronado-Gallegos, Jordan, additional, and Aréchiga-Carvajal, Elva Teresa, additional
- Published
- 2021
- Full Text
- View/download PDF
13. Analysis of the photoreceptors involved in the light-depending basidiocarp formation in Ustilago maydis
- Author
-
Sánchez-Arreguin, José Alejandro, primary, Cabrera-Ponce, José Luis, additional, León-Ramírez, Claudia Geraldine, additional, Camargo-Escalante, Martín Orlando, additional, and Ruiz-Herrera, José, additional
- Published
- 2019
- Full Text
- View/download PDF
14. Transcriptional analysis of the adaptation ofUstilago maydisduring growth under nitrogen fixation conditions
- Author
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Claudia G. León-Ramírez, Miguel Ángel Hernández-Oñate, José Alejandro Sánchez-Arreguin, and José Ruiz-Herrera
- Subjects
0106 biological sciences ,0301 basic medicine ,Nitrogen ,Ustilago ,Down-Regulation ,Secondary Metabolism ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,Gene Expression Regulation, Fungal ,Nitrogen Fixation ,Botany ,Gene expression ,Peroxisomes ,Secondary metabolism ,Nitrogen cycle ,Gene ,chemistry.chemical_classification ,Nitrates ,biology ,Gene Expression Profiling ,High-Throughput Nucleotide Sequencing ,General Medicine ,biology.organism_classification ,Actin cytoskeleton ,Adaptation, Physiological ,Actins ,Amino acid ,030104 developmental biology ,chemistry ,Biochemistry ,Nitrogen fixation ,010606 plant biology & botany - Abstract
Regulation of genes involved in nitrogen metabolism likely plays a role in the ability of fungi to exploit and survive under different environmental situations. To learn about the mechanism of adaptation of the biotrophic fungus Ustilago maydis from a medium containing a source of fixed nitrogen, to a medium depending on the ability to fix N2 by its bacterial endosymbiont, we explored gene expression profiles using RNA-Seq analyses under these two conditions. The differentially expressed (DE) fungal genes were analyzed, identifying 90 genes that were regulated 24 h after shifting the fungus to media lacking ammonium nitrate as a nitrogen source. From these, mRNA levels were increased for 49 genes, whereas 41 were down-regulated. The functional description associated to the regulated genes revealed that nine key pathways were represented, including, secondary metabolism, the metabolism of nitrogen, amino acid, fatty acid, amino sugar and nucleotide sugar, purine, peroxisome, and the regulation of actin cytoskeleton. These results suggest that the interplay of U. maydis with its N2 fixing bacterial endosymbiont is a flexible process that may be active during the adaptation of the fungus to the different nitrogen sources.
- Published
- 2017
15. Analysis of the photoreceptors involved in the light-depending basidiocarp formation in Ustilago maydis
- Author
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José Alejandro Sánchez-Arreguin, José Luis Cabrera-Ponce, Claudia G. León-Ramírez, Martín Orlando Camargo-Escalante, and José Ruiz-Herrera
- Subjects
0303 health sciences ,Phytochrome ,biology ,030306 microbiology ,Ustilago ,Chemistry ,Mutant ,General Medicine ,biology.organism_classification ,Photoreceptors, Microbial ,Biochemistry ,Microbiology ,Cell biology ,White (mutation) ,Fungal Proteins ,03 medical and health sciences ,Genetics ,Basidiocarp ,Fruiting Bodies, Fungal ,Receptor ,Molecular Biology ,Gene ,Transcription factor ,030304 developmental biology - Abstract
We have described that formation of basidiocarps by Ustilago maydis requires illumination. In the current research, we have proceeded to analyze what kind of light receptors are involved in this phenomenon. Accordingly, we investigated whether the homologues of the White Collar (WC), and the phytochrome (PHY) genes played a role in this process. Mutants deficient in either one of the three U. maydis WC homologue genes (WCO1a, WCO1b, WCO2), or the phytochrome-encoding the PHY gene were obtained. Phenotypic analysis of the mutants showed that ∆wco1a mutants formed similar numbers of basidiocarps than wild-type strain, whereas ∆wco1b mutants were severely affected in basidiocarp formation when illuminated with white, blue or red light. ∆wco2 and ∆phy1 mutants did not form basidiocarps under any illumination condition. These data indicate that Wco1a is the main blue light receptor, and Wco1b may operate as a secondary blue light receptor; Phy1 is the red light receptor, and Wco2 the transcription factor that controls the photo stimulation of the genes involved in the formation of fruiting bodies. It is suggested that effectiveness of the light receptors depends on the whole structure of the complex, possibly, because their association is necessary to maintain their functional structure.
- Published
- 2019
16. AUTOMATIZACIÓN EMBOBINADORA DE MOTORES ELÉCTRICOS.
- Author
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De La Rosa, Juan Pedro Cervantes, Muñoz Mata, José Lorenzo, Sánchez Arreguin, Judith, Saldaña González, Griselda, and Guzmán Quiroz, Ignacio
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
17. MODELADO DE UN MOTOR DE CD.
- Author
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De La Rosa, Juan Pedro Cervantes, Muñoz Mata, José Lorenzo, Rojas Garnica, Juan Carlos, Saldaña González, Griselda, and Sánchez Arreguin, Judith
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
18. Somatic Embryogenesis in Common BeanPhaseolus vulgaris L
- Author
-
Alba E. Jofre y Garfias, José Alejandro Sánchez-Arreguin, Claudia G. León-Ramírez, Itzel Anayetzi González-Gómez, and José Luis Cabrera-Ponce
- Subjects
0106 biological sciences ,0301 basic medicine ,Osmotic shock ,Somatic embryogenesis ,fungi ,Embryogenesis ,food and beverages ,Embryo ,Agrobacterium tumefaciens ,Meristem ,Biology ,biology.organism_classification ,01 natural sciences ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Cytokinin ,Phaseolus ,010606 plant biology & botany - Abstract
Common bean Phaseolus vulgaris L. has been shown to be a recalcitrant plant to induce somatic embryogenesis (SE) under in vitro conditions. An alternative strategy to yield SE is based upon the use of a cytokinin (benzyladenine) coupled with osmotic stress adaptation instead of the auxin-inducing SE in common bean. Here we described the induction of proembryogenic masses (PEM) derived from the apical meristem and cotyledonary zone of zygotic embryos, from which secondary SE indirect embryogenesis emerged. Maturation of SE was achieved by using osmotic stress medium and converted to plants. Long-term recurrent SE was demonstrated by propagation of PEM at early stages of SE. This protocol is currently being applied for stable genetic transformation by means of Agrobacterium tumefaciens and biobalistics as well as basic biochemical and molecular biology research.
- Published
- 2018
19. Generation of BSA-capsaicin Nanoparticles and Their Hormesis Effect on the Rhodotorula mucilaginosa Yeast
- Author
-
Sánchez-Arreguin, Alejandro, primary, Carriles, Ramón, additional, Ochoa-Alejo, Neftalí, additional, López, Mercedes G., additional, and Sánchez-Segura, Lino, additional
- Published
- 2019
- Full Text
- View/download PDF
20. Ustilago maydis, a Delicacy of the Aztec Cuisine and a Model for Research
- Author
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José Ruiz-Herrera, Claudia G. León-Ramírez, and José Alejandro Sánchez-Arreguin
- Subjects
Corn smut ,Nutraceutical ,biology ,Ustilago ,Delicacy ,Botany ,Basidiocarp ,food and beverages ,Ustilaginales ,Fungus ,biology.organism_classification ,Flavor - Abstract
Ustilago maydis is the causal agent of the disease known as corn smut or Huitlacohe; their natural hosts are maize (Zea mays L.) and its putative ancestor teocintle (Zea mays ssp. parviglumis and ssp. mexicana). In Mexico there is an evidence of its use as human food since pre-hispanic times. Huitlacoche is a typical Mexican food with interesting nutritional properties and distinctive flavor. Its use in cuisine is considered as a culinary delicacy in Mexico and several parts of the world and the current consumption is increasing markedly, mainly due to its exclusive flavor different from any other known food. Huitlacoche contains proteins, carbohydrates, fats, minerals and vitamins that contribute to its nutritional value, also it has been reported that it contains proteins with balanced levels of essential amino acids, something which does not occur in corn having a deficiency mostly in lysine. The fungus also contains compounds with antioxidant properties, consequently it can be included in what are now known as nutraceutical foods. Besides its use in the food, this fungus has been used in different kinds of basic investigation, such as DNA recombination, signaling, cell biology, biotrophic plant-pathogen interactions and others. Its characteristics of dimorphism, and the detailed knowledge we have on its pathogenic development, that we describe in detail, make it an ideal subject for the analysis of fungal differentiation, and for the knowledge of the behavior of the highly aggressive plant pathogens that are members of its taxonomic group, the Ustilaginales. In the present communication we make a thorough review of the nutritional characteristics of U. maydis, its life cycle, and the molecular bases of its differentiation, morpohogenesis and pathogenicity.
- Published
- 2014
21. Transcriptomic analysis of basidiocarp development in Ustilago maydis (DC) Cda
- Author
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José Luis Cabrera-Ponce, Claudia G. León-Ramírez, José Ruiz-Herrera, A. Sánchez-Arreguin, Elva T. Aréchiga-Carvajal, and Domingo Martínez-Soto
- Subjects
0301 basic medicine ,Ustilago ,030106 microbiology ,Mutant ,Fungus ,Microbiology ,Zea mays ,Transcriptome ,Fungal Proteins ,03 medical and health sciences ,Gene Expression Regulation, Fungal ,Genetics ,Fruiting Bodies, Fungal ,Gene ,Plant Diseases ,Corn smut ,biology ,Gene Expression Profiling ,Dicamba ,biology.organism_classification ,030104 developmental biology ,Basidiocarp ,Function (biology) - Abstract
Previously, we demonstrated that when Ustilago maydis (DC) Cda., a phytopathogenic basidiomycete and the causal agent of corn smut, is grown in the vicinity of maize embryogenic calli in a medium supplemented with the herbicide Dicamba, it developed gastroid-like basidiocarps. To elucidate the molecular mechanisms involved in the basidiocarp development by the fungus, we proceeded to analyze the transcriptome of the process, identifying a total of 2002 and 1064 differentially expressed genes at two developmental stages, young and mature basidiocarps, respectively. Function of these genes was analyzed with the use of different databases. MIPS analysis revealed that in the stage of young basidiocarp, among the ca. two thousand differentially expressed genes, there were some previously described for basidiocarp development in other fungal species. Additional elements that operated at this stage included, among others, genes encoding the transcription factors FOXO3, MIG3, PRO1, TEC1, copper and MFS transporters, and cytochromes P450. During mature basidiocarp development, important up-regulated genes included those encoding hydrophobins, laccases, and ferric reductase (FRE/NOX). The demonstration that a mapkk mutant was unable to form basidiocarps, indicated the importance of the MAPK signaling pathway in this developmental process.
- Published
- 2016
22. DISEÑO DE EQUIPO DE PRUEBA PARA TARJETAS ELECTRÓNICAS DE CONTROL PARA TELARES.
- Author
-
De La Rosa, Juan Pedro Cervantes, Barrientos Granda, Jorge Armando, Muñoz Mata, José Lorenzo, and Sánchez Arreguin, Judith
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
23. DIMENSIONAMIENTO DE UN SISTEMA DE ENERGÍA SOLAR FOTOVOLTAICO PARA EL EDIFICIO DE DOCENCIA DE LA UTP.
- Author
-
Sánchez Arreguin, Judith and De La Rosa, Juan Pedro Cervantes
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
24. PROTOTIPO DE SEGUIDOR SOLAR HIBRIDO.
- Author
-
Cervantes De La Rosa, Juan Pedro, Muñoz Mata, José Lorenzo, Sánchez Arreguin, Judith, and Saldaña González, Griselda
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
25. AUTOMATIZACIÓN DE UNA MÁQUINA TEJEDORA CIRCULAR CON PLC.
- Author
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De La Rosa, Juan Pedro Cervantes, Mata, José Lorenzo Muñoz, Garnica, Juan Carlos Rojas, Hernández, Alán Salvador Sombrero, and Sánchez Arreguin, Judith
- Abstract
Copyright of Congreso Internacional de Investigacion Academia Journals is the property of PDHTech, LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
26. Transcriptional analysis of the adaptation ofUstilago maydisduring growth under nitrogen fixation conditions
- Author
-
Sánchez-Arreguin, José Alejandro, primary, Hernandez-Oñate, Miguel Angel, additional, León-Ramirez, Claudia Geraldine, additional, and Ruiz-Herrera, José, additional
- Published
- 2017
- Full Text
- View/download PDF
27. Transcriptomic analysis of basidiocarp development in Ustilago maydis (DC) Cda.
- Author
-
León-Ramírez, C.G., primary, Cabrera-Ponce, J.L., additional, Martínez-Soto, D., additional, Sánchez-Arreguin, A., additional, Aréchiga-Carvajal, E.T., additional, and Ruiz-Herrera, J., additional
- Published
- 2017
- Full Text
- View/download PDF
28. Transcriptional analysis of the adaptation of Ustilago maydis during growth under nitrogen fixation conditions.
- Author
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Sánchez‐Arreguin, José Alejandro, Hernandez‐Oñate, Miguel Angel, León‐Ramirez, Claudia Geraldine, and Ruiz‐Herrera, José
- Subjects
USTILAGO maydis ,NITROGEN metabolism ,RNA sequencing ,NITROGEN fixation ,FUNGAL genes - Abstract
Regulation of genes involved in nitrogen metabolism likely plays a role in the ability of fungi to exploit and survive under different environmental situations. To learn about the mechanism of adaptation of the biotrophic fungus Ustilago maydis from a medium containing a source of fixed nitrogen, to a medium depending on the ability to fix N
2 by its bacterial endosymbiont, we explored gene expression profiles using RNA-Seq analyses under these two conditions. The differentially expressed (DE) fungal genes were analyzed, identifying 90 genes that were regulated 24 h after shifting the fungus to media lacking ammonium nitrate as a nitrogen source. From these, mRNA levels were increased for 49 genes, whereas 41 were down-regulated. The functional description associated to the regulated genes revealed that nine key pathways were represented, including, secondary metabolism, the metabolism of nitrogen, amino acid, fatty acid, amino sugar and nucleotide sugar, purine, peroxisome, and the regulation of actin cytoskeleton. These results suggest that the interplay of U. maydis with its N2 fixing bacterial endosymbiont is a flexible process that may be active during the adaptation of the fungus to the different nitrogen sources. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
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