1. Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol
- Author
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Evander de Jesus Oliveira Batista, Anderson Manoel Herculano, Mateus Santos-Silva, Tayana Silva de Carvalho, Amauri Gouveia, Waldo Lucas Luz, Sávio Lima-Bastos, Carlomagno Pacheco Bahia, Nadyme Assad, Patrick Bruno Cardoso, Alódia Brasil, Karen Renata Matos Herculano Oliveira, Suellen Alessandra Soares de Moraes, Adelaide da Conceição Fonseca Passos, and Nayara Kauffmann
- Subjects
Aging ,medicine.medical_specialty ,endocrine system ,Article Subject ,alpha-Tocopherol ,Anxiety ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Neurochemical ,Internal medicine ,Caffeine ,medicine ,Animals ,lcsh:QH573-671 ,Zebrafish ,biology ,Chemistry ,lcsh:Cytology ,High caffeine ,Cell Biology ,General Medicine ,biology.organism_classification ,030227 psychiatry ,Disease Models, Animal ,Oxidative Stress ,Endocrinology ,Female ,medicine.symptom ,030217 neurology & neurosurgery ,Oxidative stress ,Research Article - Abstract
Anxiety is a common symptom associated with high caffeine intake. Although the neurochemical mechanisms of caffeine-induced anxiety remain unclear, there are some evidences suggesting participation of oxidative stress. Based on these evidences, the current study is aimed at evaluating the possible protective effect of alpha-tocopherol (TPH) against anxiety-like behavior induced by caffeine (CAF) in zebrafish. Adult animals were treated with CAF (100 mg/kg) or TPH (1 mg/kg)+CAF before behavioral and biochemical evaluations. Oxidative stress in the zebrafish brain was evaluated by a lipid peroxidation assay, and anxiety-like behavior was monitored using light/dark preference and novel tank diving test. Caffeine treatment evoked significant elevation of brain MDA levels in the zebrafish brain, and TPH treatment prevented this increase. Caffeine treatment also induced anxiety-like behavior, while this effect was not observed in the TPH+CAF group. Taken together, the current study suggests that TPH treatment is able to inhibit oxidative stress and anxiety-like behavior evoked by caffeine.
- Published
- 2019