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1. Crop plants with improved culture and quality traits for food, feed and other uses

Author

Emmanuel Guiderdoni, Fabien Nogué, Laurence Hibrand-Saint Oyant, Philippe Vergne, Oumaya Bouchabké-Coussa, Annabelle Déjardin, Anne-Marie Chèvre, Séverine Foucrier, Christophe Sallaud, Peter M. Rogowsky, Jean-Eric Chauvin, Marianne Mazier, Pierre Barret, Pierre Devaux, Elisabeth Chevreau, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Amélioration des Plantes et Biotechnologies Végétales (APBV), Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF), Institut National de la Recherche Agronomique (INRA), Génétique et Horticulture (GenHort), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique et Amélioration des Fruits et Légumes (GAFL), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Biogemma, Chappes, France, Pépinières et Roseraies G. Delbard, Germicopa SAS, Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Groupe Sup de Co La Rochelle, Nestlé R&D Tours, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)

Subjects
0106 biological sciences, 0301 basic medicine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, F08 - Systèmes et modes de culture, agroécologie, Arabidopsis, 01 natural sciences, Plant reproduction, F30 - Génétique et amélioration des plantes, New plant breeding techniques, Sustainable agriculture, Agriculture durable, 2. Zero hunger, Gene Editing, biology, Agriculture, Phenotype, Brachypodium, CRISPR-Cas9, Genome, Plant, Biotechnology, Genome editing, Crops, Agricultural, Plant disease resistance, génomique, Crop, 03 medical and health sciences, Résistance aux facteurs nuisibles, Genetics, Animals, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, business.industry, 15. Life on land, Pesticide, biology.organism_classification, Résistance aux maladies, Bryopsida, 030104 developmental biology, Mutagenesis, Animal Science and Zoology, CRISPR-Cas Systems, business, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

The large French research project GENIUS (2012–2019, https://www6.inra.genius-project_eng/ ) provides a good showcase of current genome editing techniques applied to crop plants. It addresses a large variety of agricultural species (rice, wheat, maize, tomato, potato, oilseed rape, poplar, apple and rose) together with some models (Arabidopsis, Brachypodium, Physcomitrella). Using targeted mutagenesis as its work horse, the project is limited to proof of concept under confined conditions. It mainly covers traits linked to crop culture, such as disease resistance to viruses and fungi, flowering time, plant architecture, tolerance to salinity and plant reproduction but also addresses traits improving the quality of agricultural products for industrial purposes. Examples include virus resistant tomato, early flowering apple and low-amylose starch potato. The wide range of traits illustrates the potential of genome editing towards a more sustainable agriculture through the reduction of pesticides and to the emergence of innovative bio-economy sectors based on custom tailored quality traits.

Published
2019
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2. Improvement of in vitro donor plant competence to increase de novo shoot organogenesis in rose genotypes

Author

Séverine Foucrier, Fabrice Foucher, Romain Berruyer, M. Lecerf, Soulaiman Sakr, Linda Voisine, S. Cailleux, J. Bosselut, L. Hibrand-Saint Oyant, D. Cesbron, Laurent Ogé, Latifa Hamama, S. Pierre, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ANR-11-BTBR-0001,GENIUS,Ingénierie cellulaire : amélioration et innovation technologiques pour les plantes d'une agriculture(2011), ANR-11-BTBR-0001/11-BTBR-0001,GENIUS,Ingénierie cellulaire : amélioration et innovation technologiques pour les plantes d'une agriculture(2011), and AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA)

Subjects
0106 biological sciences, 0301 basic medicine, Sucrose, Organogenesis, SUGAR, Horticulture, Rosa, 01 natural sciences, Shoot regeneration, 03 medical and health sciences, Basal shoot, chemistry.chemical_compound, Murashige and Skoog medium, REGENERATION, LEAF EXPLANTS, Rosa chinensis, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture, SUCROSE, INVITRO SHOOT, CARBON-SOURCES, biology, GROWTH-REGULATORS, biology.organism_classification, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, 030104 developmental biology, SORBITOL D-GLUCITOL, chemistry, Shoot, Carbohydrate source KeyWords Plus:SECONDARY SOMATIC EMBRYOGENESIS, Rootstock, MICROPROPAGATION, 010606 plant biology & botany, Explant culture
Abstract

International audience; A procedure was developed for in vitro propagation of Rosa genotypes along with an efficient de novo shoot organogenesis (DNSO) method. We tested, on one genotype (hybrid of Rosa wichurana), the effects of MS basal medium complemented with two growth regulators to achieve either shoot elongation or shoot multiplication of plants. These media were complemented with carbohydrate concentrations from different sources. Then, the impacts of various carbohydrates (fructose, glucose, maltose, sorbitol, sucrose) on the growth and development of several rose genotypes during donor plant subculturing were studied on SMM. The results showed high variability in growth and development between genotypes. Contrary to other members of the Rosaceae family, no correlation was found between the shoot size and number when the amount of sorbitol was increased.Murashige and Skoog medium supplemented with 3.0 mg L-1 BAP and containing fructose or glucose at 30 g L-1 was chosen to induce leaf explants for the DNSO experiments. MS basal medium complemented with TDZ/IBA at three ratios and the same range of carbohydrate sources were tested for DNSO. Significant genotypic variations with regard to the percentage of regeneration was demonstrated with six genotypes. For two genotypes, a hybrid of Rosa wichurana and Rosa 'White Pet', we defined the conditions required to obtain 100% DNSO. For Rosa chinensis 'Old blush' and the rootstock genotype Rosa 'Natal Briar', we obtained 74 and 87.5% DNSO and only 56.67% and 37.5% for Rosa GUY SAVOY (R) ('Delstrimen') and Rosa Felicite et Perpetue' respectively. This adventitious shoot regeneration method may be used for large-scale shoot propagation and genetic engineering studies in Rosa.Keywords

Published
2019
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3. Wheat syntenome unveils new evidences of contrasted evolutionary plasticity between paleo- and neoduplicated subgenomes

Author

Catherine Feuillet, Raphael Flores, Tzion Fahima, Marco Maccaferri, Delphine Steinbach, Hadi Quesneville, Silvio Salvi, Umar Masood Quraishi, Jérôme Salse, Jaroslav Doležel, Michael Alaux, Beat Keller, Caroline Pont, Hikmet Budak, Séverine Foucrier, Sébastien Guizard, Florent Murat, Yannick Bidet, University of Zurich, Salse, Jérôme, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Unité de Recherche Génomique Info (URGI), Institut National de la Recherche Agronomique (INRA), Plateforme GINA, Université de Clermont, Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Departement of Evolutionary and Environmental Biology, University of Haifa [Haifa], Faculty of Engineering and Natural Sciences, Sabanci University [Istanbul], Institut de Biologie des Plantes, Universität Zürich [Zürich] = University of Zurich (UZH), DiSTA- Agronomia, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), DiSTA-Agronomia, INRA (Genetique et Amelioration des Plantes' reference: 'Appel d'Offre AIP Bioressources'), Auvergne region (Pole de competitivite: Cereales Vallee reference: programme 'Semences de Demain'), Agence Nationale de la Recherche (Programs ANRjc-PaleoCereal) [ANR-09-JCJC-0058-01], Agence Nationale de la Recherche (programme ANR Blanc-PAGE) [ANR-2011-BSV6-00801], European 7th Framework Programme (programme 'TRITICEAE GENOME') [FP7-212019], Institute of Experimental Botany, Universität Zürich [Zürich] (UZH), Università di Bologna [Bologna] (UNIBO), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Caroline Pont, Florent Murat, Sébastien Guizard, Raphael Flore, Séverine Foucrier, Yannick Bidet, Umar Masood Quraishi, Michael Alaux, Jaroslav Doležel, Tzion Fahima, Hikmet Budak, Beat Keller, Silvio Salvi, Marco Maccaferri, Delphine Steinbach, Catherine Feuillet, Hadi Quesneville, and Jérôme Salse

Subjects
0106 biological sciences, ble tendre, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Plant Science, 580 Plants (Botany), 01 natural sciences, paléogène, 1307 Cell Biology, 10126 Department of Plant and Microbial Biology, partitioning, 1110 Plant Science, [INFO.INFO-BT]Computer Science [cs]/Biotechnology, Conserved Sequence, Triticum, Genes, Dominant, 2. Zero hunger, Genetics, 0303 health sciences, paleogenomic, conserved orthologous set, food and beverages, Genomics, Gene conservation, gène dominant, Genome, Plant, Recombination, Autre (Sciences du Vivant), Genetic Markers, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], DNA, Plant, SNP, Biotechnologies, Plasticity, Biology, dominance, Models, Biological, Polymorphism, Single Nucleotide, Synteny, Chromosomes, Plant, Evolution, Molecular, Polyploidy, 03 medical and health sciences, 1311 Genetics, COS, Gene, 030304 developmental biology, Single nucleotide polymorphisms, Sequence Analysis, DNA, Cell Biology, paleogenomics, évolution chromosomique, 010606 plant biology & botany
Abstract

édition online; Bread wheat derives from a grass ancestor structured in 7 protochromosomes followed by a paleotetraploidization to reach a 12 chromosomes intermediate and a neohexaploidization (involving subgenomes A, B and D) event that finally shaped the 21 modern chromosomes. Insights into wheat syntenome in sequencing COS (Conserved Orthologous Set) genes unravelled differences in genomic structure (such as gene conservation and diversity) and genetical landscape (such as recombination pattern) between ancestral as well as recent duplicated blocks. Contrasted evolutionary plasticity is observed where the B subgenome appears more sensitive (i.e. plastic) in contrast to A as dominant (i.e. stable) in response to the neotetraploidization and D subgenome as supradominant (i.e. pivotal) in response to the neohexaploidization event. Finally, the wheat syntenome, delivered through a public web interface PlantSyntenyViewer at http://urgi.versailles.inra.fr/synteny-wheat, can be considered as a guide for accelerated dissection of major agronomical traits in wheat. This article is protected by copyright. All rights reserved.

Published
2013
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4. Cross-genome map based dissection of a nitrogen use efficiency ortho-metaQTL in bread wheat unravels concerted cereal genome evolution

Author

Séverine Foucrier, Gilles Charmet, Catherine Feuillet, Gregory Desmaizieres, Michael Abrouk, Alain Murigneux, Umar Masood Quraishi, Jacques Le Gouis, Caroline Pont, Nathalie Rivière, Jérôme Salse, Carole Confolent, Stéphane Lafarge, Laurent Guerreiro, Etienne Paux, and Florent Murat

Subjects
2. Zero hunger, 0106 biological sciences, Molecular breeding, Genetics, 0303 health sciences, Genome evolution, food and beverages, Locus (genetics), Cell Biology, Plant Science, Vernalization, Quantitative trait locus, Biology, 01 natural sciences, Genome, 03 medical and health sciences, Cellular localization, 030304 developmental biology, 010606 plant biology & botany, Synteny
Abstract

Monitoring nitrogen use efficiency (NUE) in plants is becoming essential to maintain yield while reducing fertilizer usage. Optimized NUE application in major crops is essential for long-term sustainability of agriculture production. Here, we report the precise identification of 11 major chromosomal regions controlling NUE in wheat that co-localise with key developmental genes such as Ppd (photoperiod sensitivity), Vrn (vernalization requirement), Rht (reduced height) and can be considered as robust markers from a molecular breeding perspective. Physical mapping, sequencing, annotation and candidate gene validation of an NUE metaQTL on wheat chromosome 3B allowed us to propose that a glutamate synthase (GoGAT) gene that is conserved structurally and functionally at orthologous positions in rice, sorghum and maize genomes may contribute to NUE in wheat and other cereals. We propose an evolutionary model for the NUE locus in cereals from a common ancestral region, involving species specific shuffling events such as gene deletion, inversion, transposition and the invasion of repetitive elements.

Published
2011
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5. The 'PUCE CAFE' Project: the First 15K Coffee Microarray, a New Tool for Discovering Candidate Genes correlated to Agronomic and Quality Traits

Author

Isabelle Privat, Séverine Foucrier, Claudine Campa, Amélie Bardil, David Pot, Alexandre de Kochko, Benoît Bertrand, Lukas A. Mueller, Philippe Lashermes, Dany Severac, Laurent Journot, Christelle Dantec, Marie-Christine Combes, Stéphane Dussert, Thierry Leroy, Ivanna Fuentes, Thierry Joët, Aureliano Bombarely Gomez, Nestlé R&D Tours, Résistance des plantes aux bio-agresseurs (UMR RPB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 2 - Sciences et Techniques (UM2), Boyce Thompson Inst Plant Res, Cornell University, Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Diversité et adaptation des plantes cultivées (UMR DIAPC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Cornell University [New York], Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0106 biological sciences, Candidate gene, lcsh:QH426-470, F60 - Physiologie et biochimie végétale, lcsh:Biotechnology, Canephora, Genomics, Coffea canephora, Coffee, Polymerase Chain Reaction, 01 natural sciences, F30 - Génétique et amélioration des plantes, 03 medical and health sciences, lcsh:TP248.13-248.65, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, ComputingMilieux_MISCELLANEOUS, Oligonucleotide Array Sequence Analysis, 030304 developmental biology, Expressed Sequence Tags, 2. Zero hunger, 0303 health sciences, Expressed sequence tag, biology, business.industry, Methodology Article, Gene Expression Profiling, Coffea arabica, Coffea eugenioides, food and beverages, Agriculture, biology.organism_classification, Biotechnology, lcsh:Genetics, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, DNA microarray, business, Functional genomics, 010606 plant biology & botany
Abstract

Background Understanding the genetic elements that contribute to key aspects of coffee biology will have an impact on future agronomical improvements for this economically important tree. During the past years, EST collections were generated in Coffee, opening the possibility to create new tools for functional genomics. Results The "PUCE CAFE" Project, organized by the scientific consortium NESTLE/IRD/CIRAD, has developed an oligo-based microarray using 15,721 unigenes derived from published coffee EST sequences mostly obtained from different stages of fruit development and leaves in Coffea Canephora (Robusta). Hybridizations for two independent experiments served to compare global gene expression profiles in three types of tissue matter (mature beans, leaves and flowers) in C. canephora as well as in the leaves of three different coffee species (C. canephora, C. eugenoides and C. arabica). Microarray construction, statistical analyses and validation by Q-PCR analysis are presented in this study. Conclusion We have generated the first 15 K coffee array during this PUCE CAFE project, granted by Génoplante (the French consortium for plant genomics). This new tool will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing bean maturation or resistance to pathogens or drought. Furthermore, the use of this array has proven to be valid in different coffee species (diploid or tetraploid), drastically enlarging its impact for high-throughput gene expression in the community of coffee research.

Published
2011
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6. Cross-genome map based dissection of a nitrogen use efficiency ortho-metaQTL in bread wheat unravels concerted cereal genome evolution

Author

Umar Masood, Quraishi, Michael, Abrouk, Florent, Murat, Caroline, Pont, Séverine, Foucrier, Gregory, Desmaizieres, Carole, Confolent, Nathalie, Rivière, Gilles, Charmet, Etienne, Paux, Alain, Murigneux, Laurent, Guerreiro, Stéphane, Lafarge, Jacques, Le Gouis, Catherine, Feuillet, and Jerome, Salse

Subjects
Evolution, Molecular, DNA, Plant, Nitrogen, Quantitative Trait Loci, Sequence Analysis, DNA, Physical Chromosome Mapping, Synteny, Chromosomes, Plant, Genome, Plant, Triticum
Abstract

Monitoring nitrogen use efficiency (NUE) in plants is becoming essential to maintain yield while reducing fertilizer usage. Optimized NUE application in major crops is essential for long-term sustainability of agriculture production. Here, we report the precise identification of 11 major chromosomal regions controlling NUE in wheat that co-localise with key developmental genes such as Ppd (photoperiod sensitivity), Vrn (vernalization requirement), Rht (reduced height) and can be considered as robust markers from a molecular breeding perspective. Physical mapping, sequencing, annotation and candidate gene validation of an NUE metaQTL on wheat chromosome 3B allowed us to propose that a glutamate synthase (GoGAT) gene that is conserved structurally and functionally at orthologous positions in rice, sorghum and maize genomes may contribute to NUE in wheat and other cereals. We propose an evolutionary model for the NUE locus in cereals from a common ancestral region, involving species specific shuffling events such as gene deletion, inversion, transposition and the invasion of repetitive elements.

Published
2011

7. Differential regulation of grain sucrose accumulation and metabolism in Coffea arabica (Arabica) and Coffea canephora (Robusta) revealed through gene expression and enzyme activity analysis

Author

Magali Eychenne, Chenwei Lin, Anneke Prins, Victoria Caillet, Steve Tanksley, Séverine Foucrier, Isabelle Privat, Thibaut Epalle, James Gerard Mccarthy, Laurianne Kandalaft, and Christine H. Foyer

Subjects
Sucrose, DNA, Complementary, Physiology, Coffea, Plant Science, Fructose, Coffea canephora, Genes, Plant, Polymerase Chain Reaction, chemistry.chemical_compound, Gene Expression Regulation, Plant, Botany, Coffee bean, biology, beta-Fructofuranosidase, Coffea arabica, fungi, food and beverages, biology.organism_classification, Plant Leaves, Invertase, Glucose, chemistry, Vacuoles, biology.protein, Sucrose synthase, Sucrose-phosphate synthase, Edible Grain
Abstract

* Coffea arabica (Arabica) and Coffea canephora (Robusta) are the two main cultivated species used for coffee bean production. Arabica genotypes generally produce a higher coffee quality than Robusta genotypes. Understanding the genetic basis for sucrose accumulation during coffee grain maturation is an important goal because sucrose is an important coffee flavor precursor. * Nine new Coffea genes encoding sucrose metabolism enzymes have been identified: sucrose phosphate synthase (CcSPS1, CcSPS2), sucrose phosphate phosphatase (CcSP1), cytoplasmic (CaInv3) and cell wall (CcInv4) invertases and four invertase inhibitors (CcInvI1, 2, 3, 4). * Activities and mRNA abundance of the sucrose metabolism enzymes were compared at different developmental stages in Arabica and Robusta grains, characterized by different sucrose contents in mature grain. * It is concluded that Robusta accumulates less sucrose than Arabica for two reasons: Robusta has higher sucrose synthase and acid invertase activities early in grain development - the expression of CcSS1 and CcInv2 appears to be crucial at this stage and Robusta has a lower SPS activity and low CcSPS1 expression at the final stages of grain development and hence has less capacity for sucrose re-synthesis. Regulation of vacuolar invertase CcInv2 activity by invertase inhibitors CcInvI2 and/or CcInvI3 during Arabica grain development is considered.

Published
2008

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