15 results on '"Saavedra Cervera, Belén"'
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2. A stool based qPCR for the diagnosis of TB in children and people living with HIV in Uganda, Eswatini and Mozambique (Stool4TB): a protocol for a multicenter diagnostic evaluation
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Carratala-Castro, Lucia, Ssengooba, Willy, Kay, Alex, Acácio, Sozinho, Ehrlich, Joanna, DiNardo, Andrew R, Shiba, Nosisa, Nsubuga, Joachim K, Munguambe, Shilzia, Saavedra-Cervera, Belén, Manjate, Patricia, Mulengwa, Durbbin, Sibandze, Busizwe, Ziyane, Mangaliso, Kasule, George, Mambuque, Edson, Sekadde, Moorine Penninah, Wobudeya, Eric, Joloba, Moses L, Heyckendorf, Jan, Lange, Christoph, Hermans, Sabine, Mandalakas, Anna, García-Basteiro, Alberto L., and Lopez-Varela, Elisa
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- 2024
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3. Monitoring of First-line Drug Resistance Mutations Outside the Scope of Xpert MTB/RIF Ultra is Needed for Successful Control of DR-TB in Southern Mozambique
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National Institutes of Health (US), European Research Council, Generalitat Valenciana, Ministerio de Ciencia e Innovación (España), Comas, Iñaki [0000-0001-5504-9408], López, Mariana G. [0000-0002-2216-9232], Torres-Puente, Manuela [0000-0002-8352-180X], Mariner-Llicer, Carla, Saavedra Cervera, Belén, Mambuque, Edson, Gomes, Neide, Munguambe, Shilzia, Villamayor, Luis, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Nguenha, Dinis, Respeito, Durval, Tembe, Gustavo, López, Mariana G., Comas, Iñaki, García-Basteiro, Alberto L., National Institutes of Health (US), European Research Council, Generalitat Valenciana, Ministerio de Ciencia e Innovación (España), Comas, Iñaki [0000-0001-5504-9408], López, Mariana G. [0000-0002-2216-9232], Torres-Puente, Manuela [0000-0002-8352-180X], Mariner-Llicer, Carla, Saavedra Cervera, Belén, Mambuque, Edson, Gomes, Neide, Munguambe, Shilzia, Villamayor, Luis, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Nguenha, Dinis, Respeito, Durval, Tembe, Gustavo, López, Mariana G., Comas, Iñaki, and García-Basteiro, Alberto L.
- Abstract
Multidrug-resistant(MDR) tuberculosis in Southern Africa is of great concern, exacerbated by the spread of a clone harboring a mutation missed by Xpert Ultra. In Southern Mozambique, the presence of such mutation and rising cases of non-MDR isoniazid resistance highlights the need to ensure accurate detection of antimicrobial-resistance in the country.
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- 2024
4. Genetic diversity within diagnostic sputum samples is mirrored in the culture of Mycobacterium tuberculosis
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Mariner-Llicer, Carla, primary, Goig, Galo A., additional, Torres-Puente, Manuela, additional, Vashakidze, Sergo, additional, Villamayor, Luis M., additional, Saavedra-Cervera, Belén, additional, Mambuque, Edson, additional, Khurtsilava, Iza, additional, Avaliani, Zaza, additional, Rosenthal, Alex, additional, Gabrielian, Andrei, additional, Shurgaia, Marika, additional, Shubladze, Natalia, additional, García-Basteiro, Alberto L., additional, López, Mariana G., additional, and Comas, Iñaki, additional
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- 2024
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5. Monitoring of First-line Drug Resistance Mutations Outside the Scope of Xpert MTB/RIF Ultra is Needed for Successful Control of DR-TB in Southern Mozambique
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Mariner-Llicer, Carla, primary, Saavedra Cervera, Belén, additional, Mambuque, Edson, additional, Gomes, Neide, additional, Munguambe, Shilzia, additional, Villamayor, Luis, additional, Cancino-Muñoz, Irving, additional, Torres-Puente, Manuela, additional, Nguenha, Dinis, additional, Respeito, Durval, additional, Tembe, Gustavo, additional, López, Mariana G, additional, Comas, Iñaki, additional, and García-Basteiro, Alberto L, additional
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- 2023
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6. Population-based surveillance of drug-resistant tuberculosis in Southern Mozambique based on whole genome sequencing
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Mariner-Llicer, Carla, primary, Saavedra-Cervera, Belén, additional, Mambuque, Edson, additional, Munguambe, Shilzia, additional, Gomes, Neide, additional, Villamayor, Luis, additional, Cancino-Muñoz, Irving, additional, Torres-Puente, Manuela, additional, Nguenha, Dinis, additional, Respeito, Durval Domingos, additional, Tembe, Gustavo, additional, Lopez, Mariana Gabriela, additional, Comas, Iñaki, additional, and Garcia-Basteiro, Alberto, additional
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- 2023
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7. Unravelling the population structure and transmission patterns of Mycobacterium tuberculosis in Mozambique, a high TB/HIV burden country
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Generalitat de Catalunya, European Commission, European Research Council, Saavedra Cervera, Belén [0000-0002-4057-0214], López, Mariana G. [0000-0002-2216-9232], Chiner-Oms, Álvaro [0000-0002-0463-0101], Cancino-Muñoz, Irving [0000-0002-5261-1634], Torres-Puente, Manuela [0000-0002-8352-180X], Mambuque, Edson [0000-0002-9106-1444], Sequera, V. G. [0000-0002-7707-1363], Respeito, Durval [0000-0002-3767-2519], Augusto, Orvalho [0000-0002-0005-3968], López-Varela, Elisa [0000-0002-6725-5687], Garcia-Basteiro, Alberto L. [0000-0002-2038-5505], Comas, Iñaki [0000-0001-5504-9408], Saavedra Cervera, Belén, López, Mariana G., Chiner-Oms, Álvaro, García Marín, Ana María, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Villamayor, Luis M., Madrazo-Moya, Carlos Francisco, Mambuque, Edson, Sequera, V. G., Respeito, Durval, Blanco, S., Augusto, Orvalho, López-Varela, Elisa, Garcia-Basteiro, Alberto L., Comas, Iñaki, Generalitat de Catalunya, European Commission, European Research Council, Saavedra Cervera, Belén [0000-0002-4057-0214], López, Mariana G. [0000-0002-2216-9232], Chiner-Oms, Álvaro [0000-0002-0463-0101], Cancino-Muñoz, Irving [0000-0002-5261-1634], Torres-Puente, Manuela [0000-0002-8352-180X], Mambuque, Edson [0000-0002-9106-1444], Sequera, V. G. [0000-0002-7707-1363], Respeito, Durval [0000-0002-3767-2519], Augusto, Orvalho [0000-0002-0005-3968], López-Varela, Elisa [0000-0002-6725-5687], Garcia-Basteiro, Alberto L. [0000-0002-2038-5505], Comas, Iñaki [0000-0001-5504-9408], Saavedra Cervera, Belén, López, Mariana G., Chiner-Oms, Álvaro, García Marín, Ana María, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Villamayor, Luis M., Madrazo-Moya, Carlos Francisco, Mambuque, Edson, Sequera, V. G., Respeito, Durval, Blanco, S., Augusto, Orvalho, López-Varela, Elisa, Garcia-Basteiro, Alberto L., and Comas, Iñaki
- Abstract
Genomic studies of Mycobacterium tuberculosis complex (MTBC) might shed light on the dynamics of its transmission, especially in high-burden settings, where recent outbreaks are embedded in the complex natural history of the disease. We applied Whole-genome sequencing (WGS) to characterize the local population of MTBC, unravel potential transmission links and evaluate associations with host and pathogen factors. Methods A one-year prospective study was conducted in Mozambique, a high HIV/TB burden country. WGS was applied to 295 positive cultures. We combined phylogenetic, geographical and clustering analysis, and investigated associations between risk factors of transmission. Findings A significant high proportion of strains were in recent transmission (45.5%). We fully characterized MTBC isolates by using phylogenetic approaches and dating evaluation. We found two likely endemic clades, comprised of 67 strains, belonging to L1.2, dating from the late XIX century and associated with recent spread among PLHIV. Interpretation Our results unveil the population structure of MTBC in our setting. The clustering analysis revealed an unexpected pattern of spread and high rates of progression, suggesting the failure of control measures. The long-term presence of local strains in Mozambique, which were responsible for large transmission among HIV/TB coinfected patients, hint at possible coevolution with sympatric host populations and challenge the role of HIV in TB transmission.
- Published
- 2022
8. Aplicaciones de la secuenciación del genoma completo de Mycobacterium tuberculosis para la predicción de resistencias a antibióticos en muestras de Mozambique
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Mariner-Llicer, Carla, Saavedra Cervera, Belén, Mambuque, Edson, Gomes, Neide, Munguambe, Shilzia, Villamayor, Luis, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Nguenha, Dinis, Respeito, Durval, Tembe, Gustavo, López, Mariana G., Comas, Iñaki, García-Basteiro, Alberto L., Mariner-Llicer, Carla, Saavedra Cervera, Belén, Mambuque, Edson, Gomes, Neide, Munguambe, Shilzia, Villamayor, Luis, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Nguenha, Dinis, Respeito, Durval, Tembe, Gustavo, López, Mariana G., Comas, Iñaki, and García-Basteiro, Alberto L.
- Published
- 2023
9. Fine-grain population structure and transmission patterns of Mycobacterium tuberculosis in southern Mozambique, a high TB/HIV burden area
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Saavedra Cervera, Belén, primary, López, Mariana G., additional, Chiner-Oms, Álvaro, additional, García, Ana María, additional, Cancino-Muñoz, Irving, additional, Torres-Puente, Manuela, additional, Villamayor, Luis, additional, Madrazo-Moya, Carlos, additional, Mambuque, Edson, additional, Sequera, Guillermo Victor, additional, Respeito, Durval, additional, Blanco, Silvia, additional, Augusto, Orvalho, additional, López-Varela, Elisa, additional, García-Basteiro, Alberto L., additional, and Comas, Iñaki, additional
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- 2022
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10. Fine-grain population structure and transmission patterns of Mycobacterium tuberculosis in southern Mozambique, a high TB/HIV burden area
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European Research Council, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, Comas, Iñaki [0000-0001-5504-9408], Cancino-Munoz, Irving [0000-0002-5261-1634], Torres-Puente, Manuela [0000-0002-8352-180X], López, Mariana G. [0000-0002-2216-9232], Chiner-Oms, Álvaro [0000-0002-0463-0101], Saavedra Cervera, Belén, López, Mariana G., Chiner-Oms, Álvaro, García Marín, Ana María, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Villamayor, Luis, Madrazo-Moya, Carlos Francisco, Mambuque, Edson, Sequera, Guillermo Víctor, Respeito, Durval, Blanco, Silvia, Augusto, Orvalho, López-Varela, Elisa, Garcia-Basteiro, Alberto L., Comas, Iñaki, European Research Council, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, Comas, Iñaki [0000-0001-5504-9408], Cancino-Munoz, Irving [0000-0002-5261-1634], Torres-Puente, Manuela [0000-0002-8352-180X], López, Mariana G. [0000-0002-2216-9232], Chiner-Oms, Álvaro [0000-0002-0463-0101], Saavedra Cervera, Belén, López, Mariana G., Chiner-Oms, Álvaro, García Marín, Ana María, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Villamayor, Luis, Madrazo-Moya, Carlos Francisco, Mambuque, Edson, Sequera, Guillermo Víctor, Respeito, Durval, Blanco, Silvia, Augusto, Orvalho, López-Varela, Elisa, Garcia-Basteiro, Alberto L., and Comas, Iñaki
- Abstract
Genomic studies of the Mycobacterium tuberculosis complex (MTBC) might shed light on the dynamics of its transmission, especially in high-burden settings, where recent outbreaks are embedded in the complex natural history of the disease. To this end, we conducted a 1 year prospective surveillance-based study in Mozambique. We applied whole-genome sequencing (WGS) to 295 positive cultures. We fully characterized MTBC isolates by phylogenetics and dating evaluation, and carried out a molecular epidemiology analysis to investigate further associations with pre-defined transmission risk factors. The majority of strains (49.5%, 136/275) belonged to lineage (L) 4; 57.8 % of them (159/275) were in genomic transmission clusters (cut-off 5 SNPs), and a strikingly high proportion (45.5%) shared an identical genotype (0 SNP pairwise distance). We found two 'likely endemic' clades, comprising 67 strains, belonging to L1.2, which dated back to the late 19th century and were associated with recent spread among people living with human immunodeficiency virus (PLHIV). We describe for the first time the population structure of MTBC in our region, a high tuberculosis (TB)/HIV burden area. Clustering analysis revealed an unforeseen pattern of spread and high rates of progression to active TB, suggesting weaknesses in TB control activities. The long-term presence of local strains in Mozambique, which were responsible for large transmission among HIV/TB-coinfected patients, calls into question the role of HIV in TB transmission.
- Published
- 2022
11. Colaboradores
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Alou Cervera, Luis, Andrade Lobato, Raquel, Arribi Vilela, Ana, Baos Muñoz, Elvira, Basaras Ibarzabal, Miren, Blázquez de Castro, Ana M.ª, Bonilla Hernández, Ignacio, Cafini Barrado, Fabio, Candel González, F. Javier, de Cueto López, Marina, Culebras López, Esther, Chiarella, Flavia C., Esteban Moreno, Jaime, Fernández Calderón, María Coronada, Fernández-Roblas, Ricardo, Fresnadillo Martínez, M.ª José, García García, Ana Belén, García García, M.ª Inmaculada, García Salguero, Cristina, García Sánchez, Enrique, González Hidalgo, Natalia, Gutiérrez Aroca, Juan Bautista, Gutiérrez Rodríguez, M.ª Purificación, Gutiérrez Zufiaurre, M.ª Nieves, Lepe Jiménez, José Antonio, Linares Sicilia, María José, López González, Laura, Maestre Vera, Juan Ramón, Morán Domínguez, Francisco Jesús, Muñoz Criado, Santiago, Palomo Lastra, María, Pena Viña, Irene, Peñuelas Martínez, Marina, Pérez Giraldo, Ciro, Ramos Tejera, Carmen, Rodríguez-Avial, Iciar, Rodríguez López, Fernando, Saavedra Cervera, Belén, Salso Ortiz, Santiago, Santana Rodríguez, Évora, Sevillano Fernández, David, Simarro Grande, María, Suárez Moya, Avelina, Trujillano Martín, Ignacio, Valle Rodríguez, José Luis, and Yagüe Guirao, Genoveva
- Published
- 2016
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12. Osteomielitis esternal y mediastinitis por Fusobacterium necrophorum.
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Saavedra-Cervera, Belén, Merino-Amador, Paloma, Collado, Lucía M., Picazo, Juan José, Hernando, Florentino, and Gómez, Ana María
- Subjects
OSTEOMYELITIS ,STERNAL gland ,FUSOBACTERIUM ,MEDIASTINITIS ,THORACIC surgery ,ESOPHAGEAL cancer ,CHEST pain ,INTERNAL medicine ,DIAGNOSIS - Published
- 2014
13. Unravelling the population structure and transmission patterns of Mycobacterium tuberculosis in Mozambique, a high TB/HIV burden country
- Author
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B. Saavedra, M.G. López, Á. Chiner-Oms, A.M. García, I Cancino, M. Torres-Puente, L. Villamayor, C. Madrazo, E. Mambuque, VG. Sequera, D. Respeito, S. Blanco, O. Augusto, E. López-Varela, AL. García-Basteiro, I. Comas, Generalitat de Catalunya, European Commission, European Research Council, Saavedra Cervera, Belén, López, Mariana G., Chiner-Oms, Álvaro, Cancino-Muñoz, Irving, Torres-Puente, Manuela, Mambuque, Edson, Sequera, V. G., Respeito, Durval, Augusto, Orvalho, López-Varela, Elisa, Garcia-Basteiro, Alberto L., Comas, Iñaki, Saavedra Cervera, Belén [0000-0002-4057-0214], López, Mariana G. [0000-0002-2216-9232], Chiner-Oms, Álvaro [0000-0002-0463-0101], Cancino-Muñoz, Irving [0000-0002-5261-1634], Torres-Puente, Manuela [0000-0002-8352-180X], Mambuque, Edson [0000-0002-9106-1444], Sequera, V. G. [0000-0002-7707-1363], Respeito, Durval [0000-0002-3767-2519], Augusto, Orvalho [0000-0002-0005-3968], López-Varela, Elisa [0000-0002-6725-5687], Garcia-Basteiro, Alberto L. [0000-0002-2038-5505], and Comas, Iñaki [0000-0001-5504-9408]
- Subjects
Molecular epidemiology ,Tuberculosis ,Transmission ,Genomics ,Mycobacterium tuberculosis - Abstract
Genomic studies of Mycobacterium tuberculosis complex (MTBC) might shed light on the dynamics of its transmission, especially in high-burden settings, where recent outbreaks are embedded in the complex natural history of the disease. We applied Whole-genome sequencing (WGS) to characterize the local population of MTBC, unravel potential transmission links and evaluate associations with host and pathogen factors. Methods A one-year prospective study was conducted in Mozambique, a high HIV/TB burden country. WGS was applied to 295 positive cultures. We combined phylogenetic, geographical and clustering analysis, and investigated associations between risk factors of transmission. Findings A significant high proportion of strains were in recent transmission (45.5%). We fully characterized MTBC isolates by using phylogenetic approaches and dating evaluation. We found two likely endemic clades, comprised of 67 strains, belonging to L1.2, dating from the late XIX century and associated with recent spread among PLHIV. Interpretation Our results unveil the population structure of MTBC in our setting. The clustering analysis revealed an unexpected pattern of spread and high rates of progression, suggesting the failure of control measures. The long-term presence of local strains in Mozambique, which were responsible for large transmission among HIV/TB coinfected patients, hint at possible coevolution with sympatric host populations and challenge the role of HIV in TB transmission., Ministry of Enterprise and Knowledge (Government of Catalonia & European Social Fund, AGAUR fellowship); European Research Council (ERC) European Union’s Horizon 2020.
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- 2022
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14. Performance of stool-based molecular tests and processing methods for paediatric tuberculosis diagnosis: a systematic review and meta-analysis.
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Carratalà-Castro L, Munguambe S, Saavedra-Cervera B, de Haas P, Kay A, Marcy O, Nabeta P, Ssengooba W, Ghimenton-Walters E, Acácio S, Bonnet M, Ehrlich J, DiNardo AR, Vasiliu A, Lange C, Hermans S, Mandalakas AM, López-Varela E, and García-Basteiro AL
- Abstract
Background: There has been a global pursuit to improve the diagnosis of tuberculosis in young children by applying diagnostic methods on accessible biospecimens such as stool. We aimed to conduct a systematic review on the accuracy of stool-based molecular tests for tuberculosis diagnosis in children and to assess the impact of the available pre-processing methods and other design characteristics., Methods: In this systematic review and meta-analysis, we evaluated studies in children younger than 16 years with presumptive tuberculosis that were published in English, Spanish, French, and Portuguese from Jan 1, 2000, to May 3, 2024, in MEDLINE, Embase, and Embase Classic, comparing the molecular detection of Mycobacterium tuberculosis DNA in stool with microbiological tests on other samples or a clinical diagnosis. We did not exclude studies based on geographical location, sample size, or study design if they were reporting primary data. Two independent reviewers (LC-C and SM) screened titles, abstracts, and full-text articles for eligibility and extracted data on study characteristics, study population, and diagnostic performance. If information relevant to the main analysis was not reported in the article, the corresponding authors were contacted. Point estimates and 95% CIs were calculated for sensitivity and specificity for each study and for the different molecular tests (Xpert MTB/RIF, Xpert Ultra MTB/RIF [Cepheid, Sunnyvale, CA, USA], and other tests) versus a reference standard (culture only, any bacteriological confirmation, and tuberculosis case definition). Sensitivity and specificity were stratified by the stool processing method. We also quantified the additionality of stool Xpert Ultra tests for tuberculosis bacteriological confirmation. The protocol was registered with PROSPERO, CRD42022341514., Findings: A total of 4521 records were identified through the database search, one record was identified from an article bibliography, and 67 studies were retained for full-text reading. 39 studies were included in the qualitative synthesis, 35 of which were included in the meta-analyses. When using any bacteriological confirmation from a respiratory sample as the reference standard, stool Xpert sensitivity was 0·60 (95% CI 0·48-0·71), stool Xpert Ultra sensitivity was 0·73 (0·63-0·81), and sensitivity was 0·44 (0·29-0·60) for other in-house molecular methods combined. When using tuberculosis case definition as the reference standard, stool Xpert sensitivity was 0·23 (0·11-0·41), stool Xpert Ultra sensitivity was 0·38 (0·22-0·56), and sensitivity was 0·17 (0·09-0·23) for other in-house molecular methods. The addition of stool Xpert Ultra increased bacteriological confirmation of tuberculosis by 38·6% overall. Further, the utilisation of centrifuge-free simplified methods improved the sensitivity of stool Xpert Ultra when using any bacteriological confirmation as a reference standard (0·77 [0·66-0·85] for centrifuge-free methods vs 0·61 [0·41-0·78] for non-centrifuge-free methods)., Interpretation: This systematic review and meta-analysis supports the use of Xpert Ultra in stool samples as a diagnostic tool for paediatric tuberculosis diagnosis. Stool-based Xpert Ultra can contribute to increase the bacteriological confirmation in this population, even when respiratory specimens are also tested., Funding: The EDCTP2 programme supported by the EU via Stool4TB Project and the European Society of Pediatric Infectious Diseases., Competing Interests: Declaration of interests MB's institution received UNITAID funding for the conduct of one of the studies included in the systematic review (Marcy et al 2022(60)). All other authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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15. Monitoring of First-line Drug Resistance Mutations Outside the Scope of Xpert MTB/RIF Ultra is Needed for Successful Control of DR-TB in Southern Mozambique.
- Author
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Mariner-Llicer C, Saavedra Cervera B, Mambuque E, Gomes N, Munguambe S, Villamayor L, Cancino-Muñoz I, Torres-Puente M, Nguenha D, Respeito D, Tembe G, López MG, Comas I, and García-Basteiro AL
- Subjects
- Humans, Rifampin pharmacology, Rifampin therapeutic use, Drug Resistance, Bacterial genetics, Mozambique, Mutation, Sensitivity and Specificity, Mycobacterium tuberculosis genetics, Tuberculosis, Multidrug-Resistant diagnosis, Tuberculosis, Multidrug-Resistant drug therapy
- Abstract
Multidrug-resistant(MDR) tuberculosis in Southern Africa is of great concern, exacerbated by the spread of a clone harboring a mutation missed by Xpert Ultra. In Southern Mozambique, the presence of such mutation and rising cases of non-MDR isoniazid resistance highlights the need to ensure accurate detection of antimicrobial-resistance in the country., Competing Interests: Potential conflicts of interest. I. C. received consultancy fees from Foundation for innovative new diagnostics for the development of the WHO mutation catalogue v1. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
- Published
- 2024
- Full Text
- View/download PDF
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