Search

Your search keyword '"Sabrina Coqueran"' showing total 9 results
Start Over Author "Sabrina Coqueran"
9 results on '"Sabrina Coqueran"'

1. Excessive self-grooming, gene dysregulation and imbalance between the striosome and matrix compartments in the striatum of Shank3 mutant mice

Author

Allain-Thibeault Ferhat, Elisabeth Verpy, Anne Biton, Benoît Forget, Fabrice De Chaumont, Florian Mueller, Anne-Marie Le Sourd, Sabrina Coqueran, Julien Schmitt, Christelle Rochefort, Laure Rondi-Reig, Aziliz Leboucher, Anne Boland, Bertrand Fin, Jean-François Deleuze, Tobias M. Boeckers, Elodie Ey, and Thomas Bourgeron

Subjects
autism, Shank3, mouse model, stereotyped behavior, striatum compartmentation, GAD65, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Autism is characterized by atypical social communication and stereotyped behaviors. Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are detected in 1–2% of patients with autism and intellectual disability, but the mechanisms underpinning the symptoms remain largely unknown. Here, we characterized the behavior of Shank3Δ11/Δ11 mice from 3 to 12 months of age. We observed decreased locomotor activity, increased stereotyped self-grooming and modification of socio-sexual interaction compared to wild-type littermates. We then used RNAseq on four brain regions of the same animals to identify differentially expressed genes (DEGs). DEGs were identified mainly in the striatum and were associated with synaptic transmission (e.g., Grm2, Dlgap1), G-protein-signaling pathways (e.g., Gnal, Prkcg1, and Camk2g), as well as excitation/inhibition balance (e.g., Gad2). Downregulated and upregulated genes were enriched in the gene clusters of medium-sized spiny neurons expressing the dopamine 1 (D1-MSN) and the dopamine 2 receptor (D2-MSN), respectively. Several DEGs (Cnr1, Gnal, Gad2, and Drd4) were reported as striosome markers. By studying the distribution of the glutamate decarboxylase GAD65, encoded by Gad2, we showed that the striosome compartment of Shank3Δ11/Δ11 mice was enlarged and displayed much higher expression of GAD65 compared to wild-type mice. Altogether, these results indicate altered gene expression in the striatum of Shank3-deficient mice and strongly suggest, for the first time, that the excessive self-grooming of these mice is related to an imbalance in the striatal striosome and matrix compartments.

Published
2023
Full Text
View/download PDF

2. Detecting Central Auditory Processing Disorders in Awake Mice

Author

Camille Dejean, Typhaine Dupont, Elisabeth Verpy, Noémi Gonçalves, Sabrina Coqueran, Nicolas Michalski, Sylvie Pucheu, Thomas Bourgeron, and Boris Gourévitch

Subjects
central auditory processing disorders, autism spectrum disorders, awake mice, auditory steady-state response, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Mice are increasingly used as models of human-acquired neurological or neurodevelopmental conditions, such as autism, schizophrenia, and Alzheimer’s disease. All these conditions involve central auditory processing disorders, which have been little investigated despite their potential for providing interesting insights into the mechanisms behind such disorders. Alterations of the auditory steady-state response to 40 Hz click trains are associated with an imbalance between neuronal excitation and inhibition, a mechanism thought to be common to many neurological disorders. Here, we demonstrate the value of presenting click trains at various rates to mice with chronically implanted pins above the inferior colliculus and the auditory cortex for obtaining easy, reliable, and long-lasting access to subcortical and cortical complex auditory processing in awake mice. Using this protocol on a mutant mouse model of autism with a defect of the Shank3 gene, we show that the neural response is impaired at high click rates (above 60 Hz) and that this impairment is visible subcortically—two results that cannot be obtained with classical protocols for cortical EEG recordings in response to stimulation at 40 Hz. These results demonstrate the value and necessity of a more complete investigation of central auditory processing disorders in mouse models of neurological or neurodevelopmental disorders.

Published
2023
Full Text
View/download PDF

3. LMT USV Toolbox, a Novel Methodological Approach to Place Mouse Ultrasonic Vocalizations in Their Behavioral Contexts—A Study in Female and Male C57BL/6J Mice and in Shank3 Mutant Females

Author

Fabrice de Chaumont, Nathalie Lemière, Sabrina Coqueran, Thomas Bourgeron, and Elodie Ey

Subjects
mouse, ultrasonic vocalization (USV), social behavior analysis, mouse model, autism, age, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Ultrasonic vocalizations (USVs) are used as a phenotypic marker in mouse models of neuropsychiatric disorders. Nevertheless, current methodologies still require time-consuming manual input or sound recordings clean of any background noise. We developed a method to overcome these two restraints to boost knowledge on mouse USVs. The methods are freely available and the USV analysis runs online at https://usv.pasteur.cloud. As little is currently known about usage and structure of ultrasonic vocalizations during social interactions over the long-term and in unconstrained context, we investigated mouse spontaneous communication by coupling the analysis of USVs with automatic labeling of behaviors. We continuously recorded during 3 days undisturbed interactions of same-sex pairs of C57BL/6J sexually naive males and females at 5 weeks and 3 and 7 months of age. In same-sex interactions, we observed robust differences between males and females in the amount of USVs produced, in the acoustic structure and in the contexts of emission. The context-specific acoustic variations emerged with increasing age. The emission of USVs also reflected a high level of excitement during social interactions. We finally highlighted the importance of studying long-term spontaneous communication by investigating female mice lacking Shank3, a synaptic protein associated with autism. While the previous short-time constrained investigations could not detect USV emission abnormalities, our analysis revealed robust differences in the usage and structure of the USVs emitted by mutant mice compared to wild-type female pairs.

Published
2021
Full Text
View/download PDF

4. Compensation between Wnt-driven tumorigenesis and cellular responses to ribosome biogenesis inhibition in the murine intestinal epithelium

Author

Sabrina Coqueran, Aurélien Raveux, Béatrice Romagnolo, Michel Cohen-Tannoudji, Sandrine Vandormael-Pournin, Aline Stedman, Nick D.L. Owens, Embryon précoce de mammifères et cellules souches, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Epigénétique des Cellules Souches - Epigenetics of Stem Cells, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Fondation ARC pour le Recherche sur le cancer (PGA120140200873), the Institut Pasteur, the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE) and the Institut National du Cancer (INCa 2007-1-COL-6-IC-1 and PLBIO09-070). A.R. was supported by the Université Pierre et Marie Curie and received fellowship from the French Ministère de l’Enseignement Supérieur et de la recherche, the Fondation ARC pour la Recherche sur le Cancer and the REVIVE Labex. AS received support from the Fondation des Treilles., Imaging analyses was performed at the Imagopole and the histopathology Unit of the Institut Pasteur. We are grateful to the staff of the animal facility of Institut Pasteur for animal care and their help during this work. We thank G. Comai for technical help and advices on RNAscope ISH., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collège Doctoral, Laboratoire de Biologie du Développement [IBPS] (LBD), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Adenomatous Polyposis Coli Protein, Ribosome biogenesis, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, medicine.disease_cause, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Intestinal Mucosa, Progenitor cell, Wnt Signaling Pathway, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Molecular Biology, Organelle Biogenesis, Wnt signaling pathway, Membrane Proteins, Cell Biology, Cell cycle, Wnt signaling, Colorectal cancer, Intestinal epithelium, 3. Good health, Cell biology, Mice, Inbred C57BL, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, Cell Transformation, Neoplastic, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer cell, Stem cell, Carcinogenesis, Ribosomes
Abstract

International audience; Ribosome biogenesis inhibition causes cell cycle arrest and apoptosis through the activation of tumor suppressor-dependent surveillance pathways. These responses are exacerbated in cancer cells, suggesting that targeting ribosome synthesis may be beneficial to patients. Here, we characterize the effect of the loss-of-function of Notchless (Nle), an essential actor of ribosome biogenesis, on the intestinal epithelium undergoing tumor initiation due to acute Apc loss-of-function. We show that ribosome biogenesis dysfunction strongly alleviates Wnt-driven tumor initiation by restoring cell cycle exit and differentiation in Apc-deficient progenitors. Conversely Wnt hyperactivation attenuates the cellular responses to surveillance pathways activation induced by ribosome biogenesis dysfunction, as proliferation was maintained at control-like levels in the stem cells and progenitors of double mutants. Thus, our data indicate that, while ribosome biogenesis inhibition efficiently reduces cancer cell proliferation in the intestinal epithelium, enhanced resistance of Apc-deficient stem and progenitor cells to ribosome biogenesis defects may be an important concern when using a therapeutic strategy targeting ribosome production for the treatment of Wnt-dependent tumorigenesis.

Published
2020

5. Excessive self-grooming, gene dysregulation and imbalance between the striosome and matrix compartments in the striatum ofShank3mutant mice

Author

Allain-Thibeault, Ferhat, primary, Elisabeth, Verpy, additional, Anne, Biton, additional, Benoît, Forget, additional, de Chaumont, Fabrice, additional, Florian, Mueller, additional, Anne-Marie, Le Sourd, additional, Sabrina, Coqueran, additional, Julien, Schmitt, additional, Christelle, Rochefort, additional, Laure, Rondi-Reig, additional, Aziliz, Leboucher, additional, Anne, Boland, additional, Bertrand, Fin, additional, François, Deleuze Jean, additional, Boeckers, Tobias M., additional, Elodie, Ey, additional, and Thomas, Bourgeron, additional

Published
2022
Full Text
View/download PDF

6. PDGF signaling in primitive endoderm cell survival is mediated by PI3K-mTOR through p53-independent mechanism

Author

Sabrina Coqueran, Michel Cohen-Tannoudji, Sylvain Bessonnard, Jérôme Artus, Sandrine Vandormael-Pournin, Embryon précoce de mammifères et cellules souches, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE and ANR-14CE11-0017 PrEpiSpec). S.B. was supported by the Fondation pour laRecherche Médicale (ARF20150934222). J.A. was supported by the European program Marie Curie (International Incoming Fellowship, Seventh European Community Framework Programme)., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-14-CE11-0017,Prepispec,Mécanismes moléculaires de la spécification des lignages cellulaires pendant l'embryogenèse précoce de la souris(2014), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Male, Receptor, Platelet-Derived Growth Factor alpha, Cell Survival, Cell, Fibroblast growth factor, Receptor tyrosine kinase, 03 medical and health sciences, Mice, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, PDGF signaling, medicine, Animals, Cell Lineage, [SDV.BDD]Life Sciences [q-bio]/Development Biology, PI3K/AKT/mTOR pathway, primitive endoderm, Platelet-Derived Growth Factor, mouse embryo, biology, blastocyst, TOR Serine-Threonine Kinases, Endoderm, apoptosis, Gene Expression Regulation, Developmental, Cell Biology, Cell biology, Fibroblast Growth Factors, Proto-Oncogene Proteins c-kit, 030104 developmental biology, medicine.anatomical_structure, Epiblast, Blastocyst Inner Cell Mass, embryonic structures, biology.protein, Molecular Medicine, Female, Signal transduction, Stem cell, Tumor Suppressor Protein p53, 030217 neurology & neurosurgery, Platelet-derived growth factor receptor, Developmental Biology, Signal Transduction
Abstract

International audience; Receptor tyrosine kinase signaling (RTK) are key regulators of the formation of the primitive endoderm (PrE) and the epiblast (Epi) from the inner cell mass of the mouse preimplantation embryo. Among them, FGF signaling is critical for PrE cell specification while PDGF signaling is critical for the survival of committed PrE cells. Here, we investigated possible functional redundancies between FGF, PDGF and KIT signaling and showed that only PDGF signaling is involved in PrE cell survival. In addition, we analyzed the effectors downstream of PDGFRα. Our results suggest that the role of PDGF signaling in PrE cell survival is mediated through PI3K-mTOR and independently from p53. Lastly, we uncovered a role for PI3K-mTOR signaling in the survival of Epi cells. Taken together, we propose that survival of ICM cell lineages relies on the regulation of PI3K-mTOR signaling through the regulation of multiple signaling pathways. SIGNIFICANCE STATEMENT: Segregation between embryonic and extra-embryonic lineages occurs at the beginning of mammalian development and requires multiple processes participating in the acquisition and the maintenance of cell identities. In this study, we have characterized the intracellular factors involved in the survival of the epiblast, that will form the embryo proper, and the primitive endoderm, an extraembryonic lineage participating to the formation of the yolk sac, just after their formation. Such better understanding of early embryonic development could benefit to the fields of stem cell biology and assisted reproduction technologies. © AlphaMed Press 2019.

Published
2019

7. Mouse adult hematopoietic stem cells actively synthetize ribosomal RNA

Author

Ana Cumano, Alexandre David, Sandrine Vandormael-Pournin, Sabrina Coqueran, Michel Cohen-Tannoudji, Marie Le Bouteiller, Aurélien Raveux, Léonard Jarzebowski, Embryon précoce de mammifères et cellules souches, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Développement des Lymphocytes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique, and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE). L.J. was supported by the DIM biotherapies Ile-de-France, the Société Française d'Hématologie, and the REVIVE Labex. M.L.B. was supported by the Ligue Nationale Contre le Cancer, Imaging and cell sorting analyses were performed at the Imagopole of Institut Pasteur. We are grateful to P.-H. Commere for his assistance with FACS analysis and sorting. We thank F. Morlé, L. Da Costa, and all members of the laboratory for technical advice and helpful discussions., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
0301 basic medicine, Ribosomal Proteins, Cell, Ribosome biogenesis, Biology, rRNA maturation, Article, 03 medical and health sciences, Mice, medicine, RNA Precursors, Animals, Progenitor cell, RNA Processing, Post-Transcriptional, Molecular Biology, Tissue homeostasis, Hematopoietic stem cell, Cell Differentiation, Hematopoietic Stem Cell, Ribosomal RNA, Hematopoietic Stem Cells, Cell biology, Haematopoiesis, 030104 developmental biology, medicine.anatomical_structure, RNA, Ribosomal, [SDV.IMM]Life Sciences [q-bio]/Immunology, Stem cell, Ribosomes
Abstract

International audience; Contribution of basal cellular processes to the regulation of tissue homeostasis has just started to be appreciated. However, our knowledge on the modulation of ribosome biogenesis activity in situ within specific lineages remains very limited. This is largely due to the lack of assays that enable quantitation of ribosome biogenesis in small numbers of cells in vivo. We used a technique, named Flow-FISH, combining cell surface antibody staining and flow cytometry with intracellular ribosomal RNA (rRNA) FISH, to measure the levels of pre-rRNAs of hematopoietic cells in vivo. Here, we show that Flow-FISH reports and quantifies ribosome biogenesis activity in hematopoietic cell populations, thereby providing original data on this fundamental process notably in rare populations such as hematopoietic stem and progenitor cells. We unravel variations in pre-rRNA levels between different hematopoietic progenitor compartments and during erythroid differentiation. In particular, our data indicate that, contrary to what may be anticipated from their quiescent state, hematopoietic stem cells have significant ribosome biogenesis activity. Moreover, variations in pre-rRNA levels do not correlate with proliferation rate suggesting that cell type specific mechanisms might regulate ribosome biogenesis in hematopoietic stem cells and progenitors. Our study contributes to a better understanding of the cellular physiology of the hematopoietic system in vivo in unperturbed situation.

Published
2018

8. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation

Author

Sabrina Coqueran, Jérôme Artus, Sylvain Bessonnard, Sandrine Vandormael-Pournin, Alexandre Dufour, Michel Cohen-Tannoudji, Génétique fonctionnelle de la Souris, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Analyse d'images biologiques - Biological Image Analysis (BIA), Université Paris-Sud - Paris 11 - Faculté de médecine (UP11 UFR Médecine), Université Paris-Sud - Paris 11 (UP11), This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE and ANR-14CE11-0017 PrEpiSpec). S.B. was supported by the Fondation pour la Recherche Médicale (ARF20150934222). J.A. was supported by the European program Marie Curie (International Incoming Fellowship, Seventh European Community Framework Programme)., Imaging was performed for most parts at the Imagopole of Institut., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-14-CE11-0017,Prepispec,Mécanismes moléculaires de la spécification des lignages cellulaires pendant l'embryogenèse précoce de la souris(2014), European Project: 291784,EC:FP7:PEOPLE,FP7-PEOPLE-2011-COFUND,ZIF-MARIE-CURIE(2012), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
MAPK/ERK pathway, Time Factors, Transcription, Genetic, MAP Kinase Signaling System, Cell Plasticity, lcsh:Medicine, Biology, Protein degradation, Fibroblast growth factor, Bioinformatics, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Animals, Inner cell mass, Progenitor cell, lcsh:Science, [SDV.BDD]Life Sciences [q-bio]/Development Biology, reproductive and urinary physiology, 030304 developmental biology, 0303 health sciences, lcsh:R, Cell Differentiation, Cell biology, Fibroblast Growth Factors, Epiblast, Blastocyst Inner Cell Mass, Proteolysis, embryonic structures, lcsh:Q, Developmental biology, Germ Layers, 030217 neurology & neurosurgery
Abstract

International audience; Inner cell Mass (ICM) specification into epiblast (Epi) and primitive endoderm (PrE) is an asynchronous and progressive process taking place between E3.0 to E3.75 under the control of the Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway. Here, we have analyzed in details the kinetics of specification and found that ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. We also showed that PrE progenitors are generated later than Epi progenitors. We further demonstrated that, during this late phase of specification, a 4 hours period of FGF/ERK inhibition prior E3.75 is sufficient to convert ICM cells into Epi. Finally, we showed that ICM conversion into Epi in response to inhibition during this short time window requires both transcription and proteasome degradation. Collectively, our data give new insights into the timing and mechanisms involved in the process of ICM specification.

Published
2017

9. Progressive developmental restriction, acquisition of left-right identity, and cell growth behavior during lobe formation in mouse liver development

Author

Mary C. Weiss, Helene Strick-Marchand, Margaret Buckingham, Sabrina Coqueran, and Jean-François Le Garrec

Subjects
0301 basic medicine, Organogenesis, Morphogenesis, Mice, Transgenic, Biology, Mice, 03 medical and health sciences, medicine, Animals, Gene Knock-In Techniques, Progenitor cell, Molecular Biology, Cells, Cultured, Body Patterning, Cell Proliferation, Retrospective Studies, Genetics, Cell growth, Stem Cells, beta-Galactosidase, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Hepatocyte Nuclear Factor 4, Lac Operon, Liver, Hepatocyte nuclear factor 4, Liver Lobe, Mice, Inbred DBA, Hepatocytes, Hepatic stellate cell, Endoderm, Stem cell, Developmental Biology
Abstract

To identify cell-based decisions implicated in morphogenesis of the mammalian liver, we performed clonal analysis of hepatocytes/hepatoblasts in mouse liver development, using a knock-in allele of HNF4α/laacZ. This transgene randomly undergoes a low frequency of recombination that generates a functional lacZ gene that produces β-galactosidase in tissues where HNF4α is expressed. Two types of β-galactosidase-positive clones were found. Most have undergone 3-8 cell divisions and result from independent events (Luria-Delbruck fluctuation test); we calculate that they arose between E8.5 and E13.5. A second class was mega-clones derived from early endoderm progenitors, generating many descendants. Some originated from multi-potential founder cells, with labeled cells in the liver, pancreas and/or intestine. A few mega-clones populate only one side of the liver, indicating hepatic cell chirality. The patterns of labeled cells indicate cohesive and often oriented growth, notably in broad radial stripes, potentially implicated in the formation of liver lobes. This retrospective clonal analysis gives novel insights into clonal origins, cell behavior of progenitors and distinct properties of endoderm cells that underlie the formation and morphogenesis of the liver.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results