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35 results on '"Sacerdoti, Flavia"'

7. EFFECTS OF BACTERIAL LIPOPOLYSACCHARIDE AND SHIGA TOXIN ON INDUCED PLURIPOTENT STEM CELL-DERIVED MESENCHYMAL STEM CELLS

Author

Martire-Greco, Daiana, La Greca, Alejandro, Castillo Montañez, Luis, Biani, Celeste, Lombardi, Antonella, Birnberg-Weiss, Federico, Norris, Alessandra, Sacerdoti, Flavia, Amaral, María Marta, Rodrigues-Rodriguez, Nahuel, Pittaluga, Jose Ramón, Furmento, Verónica Alejandra, Landoni, Verónica Inés, Miriuka, Santiago Gabriel, Luzzani, Carlos, and Fernández, Gabriela Cristina

Published
2023
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13. Detection of Shiga Toxin-Producing Escherichia coli (STEC) in the Endocervix of Asymptomatic Pregnant Women. Can STEC Be a Risk Factor for Adverse Pregnancy Outcomes?

Author

Scalise, María Luján, primary, Garimano, Nicolás, additional, Sanz, Marcelo, additional, Padola, Nora Lia, additional, Leonino, Patricia, additional, Pereyra, Adriana, additional, Casale, Roberto, additional, Amaral, María Marta, additional, Sacerdoti, Flavia, additional, and Ibarra, Cristina, additional

Published
2022
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16. The Deleterious Effects of Shiga Toxin Type 2 Are Neutralized In Vitro by FabF8:Stx2 Recombinant Monoclonal Antibody

Author

Luz, Daniela, primary, Gómez, Fernando D., additional, Ferreira, Raíssa L., additional, Melo, Bruna S., additional, Guth, Beatriz E. C., additional, Quintilio, Wagner, additional, Moro, Ana Maria, additional, Presta, Agostina, additional, Sacerdoti, Flavia, additional, Ibarra, Cristina, additional, Chen, Gang, additional, Sidhu, Sachdev S., additional, Amaral, María Marta, additional, and Piazza, Roxane M. F., additional

Published
2021
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17. Effects of bacterial lipopolysaccharide and Shiga Toxin on induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells

Author

Martire-Greco, Daiana, primary, La Greca, Alejandro, additional, Castillo Montañez, Luis, additional, Biani, Celeste, additional, Lombardi, Antonella, additional, Birnberg-Weiss, Federico, additional, Norris, Alessandra, additional, Sacerdoti, Flavia, additional, Amaral, María Marta, additional, Rodrigues-Rodriguez, Nahuel, additional, Pittaluga, José Ramón, additional, Furmento, Verónica Alejandra, additional, Landoni, Verónica Inés, additional, Miriuka, Santiago Gabriel, additional, Luzzani, Carlos D, additional, and Fernández, Gabriela Cristina, additional

Published
2021
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20. Detection of Shiga Toxin-Producing Escherichia coli (STEC) in the Endocervix of Asymptomatic Pregnant Women. Can STEC Be a Risk Factor for Adverse Pregnancy Outcomes?

Author

Luján Scalise, María, Garimano, Nicolás, Sanz, Marcelo, Lia Padola, Nora, Leonino, Patricia, Pereyra, Adriana, Casale, Roberto, Marta Amaral, María, Sacerdoti, Flavia, and Ibarra, Cristina

Abstract

The presence of Escherichia coli in the vaginal microbiome has been associated with pregnancy complications. In previous works, we demonstrated that Shiga toxin-producing Escherichia coli (STEC) can produce abortion and premature delivery in rats and that Shiga toxin type 2 (Stx2) can impair human trophoblast cell lines. The hypothesis of this work was that STEC may colonize the lower female reproductive tract and be responsible for adverse pregnancy outcomes. Thus, the aim of this work was to evaluate the presence and prevalence of virulence factor genes from STEC in the endocervix of asymptomatic pregnant women. For that purpose, endocervical swabs were collected from pregnant women during their prenatal examination. Swab samples were enriched in a differential medium to select Enterobacteria. Then, positive samples were analyzed by PCR to detect genes characteristic of Escherichia sp. (such as uidA and yaiO), genes specific for portions of the rfb (O-antigen-encoding) regions of STEC O157 (rfbO157), and STEC virulence factor genes (such as stx1, stx2, eae, lpfAO113, hcpA, iha, sab, subAB). The cytotoxic effects of stx2-positive supernatants from E. coli recovered from the endocervix were evaluated in Vero cells. Our results showed that 11.7% of the endocervical samples were positive for E. coli. Additionally, we found samples positive for stx2 and other virulence factors for STEC. The bacterial supernatant from an isolate identified as E. coli O113:NT, carrying the stx2 gene, exhibited cytotoxic activity in Vero, Swan 71 and Hela cells. Our results open a new perspective regarding the presence of STEC during pregnancy. [ABSTRACT FROM AUTHOR]

Published
2022
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22. The limited knowledge of placental damage due to neglected infections: ongoing problems in Latin America

Author

Ribeiro, Isabela Moreti, primary, Souto, Paula Cristina Souza, additional, Borbely, Alexandre U., additional, Tanabe, Eloiza Lopes Lira, additional, Cadavid, Angela, additional, Alvarez, Angela M., additional, Bueno, Julio, additional, Agudelo, Olga, additional, Robles, Reggie García, additional, Ayala-Ramírez, Paola, additional, Sacerdoti, Flavia, additional, Szasz, Theodora, additional, Damiano, Alicia E., additional, Ibarra, Cristina, additional, Escudero, Carlos, additional, Lima, Victor V., additional, and Giachini, Fernanda R., additional

Published
2020
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34. Detection of plasma anti-lipopolysaccharide (LPS) antibodies against enterohemorrhagic E. coli(EHEC) in asymptomatic kindergarten teachers from Buenos Aires province

Author

Fernandez-Brando, Romina J., Sacerdoti, Flavia, Amaral, María M., Bernal, Alan M., Da Rocha, Marcelo, Belardo, Marcela, Palermo, Marina S., and Ibarra, Cristina A.

Abstract

•Fifty-one percent of teachers had circulating antibodies against O157, O145, O121 and O103 LPS.•Anti-O157 antibodies were the most abundant found in 43% of the samples.•Twenty-one of the samples reacted against more than one LPS serotype.•Twenty percent showed IgM antibodies against at least one LPS serotype.•Continuous educational programs could help to reduce cases of HUS.

Published
2023
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35. Involvement of aquaporins in Shiga toxin-induced swelling and water transport dysfunction in human renal microvascular endothelial cells.

Author

Gomez FD, Reppetti J, Alvarez RS, Girón Reyes DC, Sacerdoti F, Balestracci A, Damiano AE, Martínez NA, Di Giusto G, and Amaral MM

Abstract

One of the hallmarks of Shiga toxin-producing Escherichia coli-associated hemolytic uremic syndrome (STEC-HUS) is kidney damage. Our previous research demonstrated that Shiga toxin type 2 (Stx2a) decreases cell viability and induces swelling of human glomerular endothelial cells (HGEC). However, Stx2a can disrupt net water transport across HGEC monolayers without affecting cell viability. This work aimed to elucidate the possible mechanisms involved in the water transport disruption caused by Stx2a across HGEC monolayers. We investigated paracellular and transcellular water transfer across HGEC by analyzing the passage of FITC-Dextran and the hydrostatic pressure (Phydr) and measuring the osmotic pressure (Posm), respectively. Stx2a selectively affected the transcellular pathway without impacting the paracellular route. Furthermore, Stx2a cell swelling was prevented by pretreatment with aquaporin inhibitors tetraethylammonium chloride (TEA), Mercury (II) chloride (HgCl 2 ) or TGN-020, suggesting aquaporin involvement in this process. Confocal microscopy revealed that Stx2a increased HGEC total volume, which TEA and TGN-020 counteracted. Additionally, we identified in HGEC not only the expression of aquaporin-1 (AQP1) but also the expression of aquaporin-4 (AQP4). Surprisingly, we observed a decrease in the expression of both AQPs after Stx2a exposure. Our findings suggest that Stx2a may induce water movement into HGEC via AQP1 and AQP4, increasing total cell volume. Subsequently, decreased AQP1 and AQP4 expression could inhibit transcellular water transfer, potentially as a protective mechanism against excessive water entry and cell lysis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Published
2024
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