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Infectious laryngotracheitis (ILT) is an acute contagious upper respiratory tract infection of ‎chickens and other birds, caused by Gallid herpesvirus1 (GaHV-1), which has economic ‎importance in the poultry industry. There was no scientific data about the incidence of the ‎disease in broiler farms in Sulaymaniyah province/Iraq. Therefore, this study aimed to ‎investigate ILTV infection in broiler farms in that region. Clinically infected birds from 89 ‎broiler flocks that had respiratory distress, coughing, gasping, tracheal rales, nasal ocular ‎discharge, and congested trachea with purulent exudate, hemorrhagic tracheitis with/or without ‎necrotic changes were investigated. The DNA was extracted from the pooled samples, ‎including tracheal secretion, trachea, and lung tissue. Primers specific to the thymidine kinase ‎gene (tdk) of ILTV- were used in PCR to detect the virus. A phylogenic tree was generated to ‎track the virus’s origin. The study revealed that the rate of infection with ILTV among broiler ‎farms was 2.2% (2/89) in the region. The sequencing analysis showed that the ILTV isolated in ‎the area was closely related to the reported strains in the United States and Brazil (MN643591.1 ‎and S83714.1); and had a sequence identity of 98.27% to the taxon ‎JQ217378.1. In conclusion, the study reported that one of the causes of the respiratory viral ‎infection in broiler flocks even at younger ages was related to ILTV. Partially sequenced tdk ‎gene of the virus showed that the circulated serovar in the region had some nucleotides and ‎amino acids differences with the worldwide reported serovars. This should be taken into ‎consideration in the poultry industry by doing further investigation‎.

Bone marrow, a soft spongy tissue, is containing mesenchymal stem cells, that are well-recognized according to their self-renewability and stemness. Therefore, we hypothesized that bone marrow aspirate concentrate (BMAC) could have a pivotal influence on the process of wound healing in particular when it is combined with platelet-rich plasma (PRP). Thirty-six albino mice (BALB/c) were used in the study and they were grouped as negative-control, PRP treated, BMAC treated and BMAC plus PRP treated. An incisional wound (1 cm2) was made at the back of mouse and their wounds were treated according to their treatment plan and group allocations. Later, the skin at the treated wound sites was collected on days 7, 14, and 21 for histopathological investigation. The results showed that there was a statistically significant difference in BMAC+PRP-treated wounds over the rest of the treated groups in the acceleration of wound healing throughout the experiment by increasing the rate of wound contraction, re-epithelization process, and granulation tissue intensity with fluctuated infiltration in the number of the neutrophils, macrophages, and lymphocytes, also restoration of the epidermal and dermal thickness with less scarring and hair follicle regeneration vs to the negative-control, PRP and BMAC only treated groups. Our findings indicated that BMAC containing mesenchymal stem cells is an efficient approach, which can be used to enhance a smooth and physiopathological healing process, especially when it is used in combination with PRP. [ABSTRACT FROM AUTHOR]