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Migrating cells often encounter a wide variety of topographic features-including the presence of obstacles-when navigating through crowded biological environments. Unraveling the impact of topography and crowding on the dynamics of cells is key to better understand many essential physiological processes such as the immune response. We study the impact of geometrical cues on ameboid migration of HL-60 cells differentiated into neutrophils. A microfluidic device is designed to track the cells in confining geometries between two parallel plates with distance h, in which identical micropillars are arranged in regular pillar forests with pillar spacing e. We observe that the cells are temporarily captured near pillars, with a mean contact time that is independent of h and e. By decreasing the vertical confinement h, we find that the cell velocity is not affected, while the persistence reduces; thus, cells are able to preserve their velocity when highly squeezed but lose the ability to control their direction of motion. At a given h, we show that by decreasing the pillar spacing e in the weak lateral confinement regime, the mean escape time of cells from effective local traps between neighboring pillars grows. This effect, together with the increase of cell-pillar contact frequency, leads to the reduction of diffusion constant D. By disentangling the contributions of these two effects on D in numerical simulations, we verify that the impact of cell-pillar contacts on cell diffusivity is more pronounced at smaller pillar spacing., Competing Interests: Declaration of interests The authors declare no conflict of interest., (Copyright © 2022 Biophysical Society. Published by Elsevier Inc. All rights reserved.)

We study the stochastic motion of active particles that undergo spontaneous transitions between two distinct modes of motion. Each mode is characterized by a velocity distribution and an arbitrary (anti)persistence. We present an analytical formalism to provide a quantitative link between these two microscopic statistical properties of the trajectory and macroscopically observable transport quantities of interest. For exponentially distributed residence times in each state, we derive analytical expressions for the initial anomalous exponent, the characteristic crossover time to the asymptotic diffusive dynamics, and the long-term diffusion constant. We also obtain an exact expression for the time evolution of the mean square displacement over all timescales and provide a recipe to obtain higher displacement moments. Our approach enables us to disentangle the combined effects of velocity, persistence, and switching probabilities between the two states on the kinematics of particles in a wide range of stochastic active or passive processes and to optimize the transport quantities of interest with respect to any of the particle dynamics properties.

The orientational memory of particles can serve as an effective measure of diffusivity, spreading, and search efficiency in complex stochastic processes. We develop a theoretical framework to describe the decay of directional correlations in a generic class of stochastic active processes consisting of distinct states of motion characterized by their persistence and switching probabilities between the states. For exponentially distributed sojourn times, the orientation autocorrelation is analytically derived and the characteristic times of its crossovers are obtained in terms of the persistence of each state and the switching probabilities. We show how nonexponential sojourn-time distributions of interest, such as Gaussian and power-law distributions, can result from history-dependent transitions between the states. The relaxation behavior of the correlation function in such non-Markovian processes is governed by the history dependence of the switching probabilities and cannot be solely determined by the mean sojourn times of the states.

The motion of self-propelled particles is modeled as a persistent random walk. An analytical framework is developed that allows the derivation of exact expressions for the time evolution of arbitrary moments of the persistent walk's displacement. It is shown that the interplay of step length and turning angle distributions and self-propulsion produces various signs of anomalous diffusion at short time scales and asymptotically a normal diffusion behavior with a broad range of diffusion coefficients. The crossover from the anomalous short-time behavior to the asymptotic diffusion regime is studied and the parameter dependencies of the crossover time are discussed. Higher moments of the displacement distribution are calculated and analytical expressions for the time evolution of the skewness and the kurtosis of the distribution are presented.

The capillary rise of liquid in asymmetric channel junctions with branches of different radii can lead to long-lasting meniscus arrests in the wider channel, which has important implications for the morphology and dynamical broadening of imbibition fronts in porous materials with elongated pores. Using a microfluidic setup, we experimentally demonstrate the existence of arrest events in Y-shaped junctions, and measure their duration and compare them with theoretical predictions. For various ratios of the channel width and liquid viscosities and for different values of the feeding channel length, we find that the meniscus within the wider branch is arrested for a time that is proportional to the time that the meniscus needed to reach the junction, in very good quantitative agreement with theoretical predictions.

We theoretically study the transport properties of self-propelled particles on complex structures, such as motor proteins on filament networks. A general master equation formalism is developed to investigate the persistent motion of individual random walkers, which enables us to identify the contributions of key parameters: the motor processivity, and the anisotropy and heterogeneity of the underlying network. We prove the existence of different dynamical regimes of anomalous motion, and that the crossover times between these regimes as well as the asymptotic diffusion coefficient can be increased by several orders of magnitude within biologically relevant control parameter ranges. In terms of motion in continuous space, the interplay between stepping strategy and persistency of the walker is established as a source of anomalous diffusion at short and intermediate time scales.

Recently, anomalous scaling properties of front broadening during spontaneous imbibition of water in Vycor glass, a nanoporous medium, were reported: the mean height and the width of the propagating front increase with time t both proportional to t(1/2). Here, we propose a simple lattice imbibition model and elucidate quantitatively how the correlation range of the hydrostatic pressure and the disorder strength of the pore radii affect the scaling properties of the imbibition front. We introduce an effective tension of liquid across neighboring pores, which depends on the aspect ratio of each pore, and show that it leads to a dynamical crossover: both the mean height and the roughness grow faster in the presence of tension in the intermediate-time regime but eventually saturate in the long-time regime. The universality class of the long-time behavior is discussed by examining the associated scaling exponents and their relation to directed percolation.

We present a scaling theory for the long time behavior of spontaneous imbibition in porous media consisting of interconnected pores with a large length-to-width ratio. At pore junctions, the meniscus propagation in one or more branches can come to a halt when the Laplace pressure of the meniscus exceeds the hydrostatic pressure within the junction. We derive the scaling relations for the emerging arrest time distribution and show that the average front width is proportional to the height, yielding a roughness exponent of exactly β = 1/2 and explaining recent experimental results for nanoporous Vycor glass. Extensive simulations of a pore network model confirm these predictions.

During spontaneous imbibition, a wetting liquid is drawn into a porous medium by capillary forces. In systems with comparable pore length and diameter, such as paper and sand, the front of the propagating liquid forms a continuous interface. Sections of this interface advance in a highly correlated manner due to an effective surface tension, which restricts front broadening. Here we investigate water imbibition in a nanoporous glass (Vycor) in which the pores are much longer than they are wide. In this case, no continuous liquid-vapor interface with coalesced menisci can form. Anomalously fast imbibition front roughening is experimentally observed by neutron imaging. We propose a theoretical pore-network model, whose structural details are adapted to the microscopic pore structure of Vycor glass and show that it displays the same large-scale roughening characteristics as observed in the experiment. The model predicts that menisci movements are uncorrelated, indicating that despite the connectivity of the network the smoothening effect of surface tension on the imbibition front roughening is negligible. These results suggest a new universality class of imbibition behavior, which is expected to occur in any matrix with elongated, interconnected pores of random radii.

We study photon diffusion in a two-dimensional random packing of monodisperse disks as a simple model of granular material. We apply ray optics approximation to set up a persistent random walk for the photons. We employ Fresnel's intensity reflectance with its rich dependence on the incidence angle and polarization state of the light. We present an analytic expression for the transport-mean-free path l* in terms of the refractive indices of grains and host medium, grain radius, and packing fraction. We perform numerical simulations to examine our analytical result.

We study the persistent random walk of photons on a one-dimensional lattice of random asymmetric transmittances. Each site is characterized by its intensity transmittance t (t;{'} not equalt) for photons moving to the right (left) direction. Transmittances at different sites are assumed independent, distributed according to a given probability density F(t,t;{'}) . We use the effective medium approximation and identify two classes of F(t,t;{'}) which lead to the normal diffusion of photons. Monte Carlo simulations confirm our predictions. We mention that the metamaterial introduced by Fedetov [Nano Lett. 7, 1996 (2007)] can be used to realize a lattice of random asymmetric transmittances.

We study photon diffusion in a two-dimensional random packing of monodisperse disks as a simple model of granular media and wet foams. We assume that the intensity reflectance of disks is a constant r . We present an analytic expression for the transport mean free path l;{*} in terms of the velocity of light in the disks and host medium, radius R and packing fraction of the disks, and the intensity reflectance. For glass beads immersed in air or water, we estimate transport mean free paths about half the experimental ones. For air bubbles immersed in water, l;{*}R is a linear function of 1epsilon , where epsilon is the liquid volume fraction of the model wet foam. This throws light on the empirical law of Vera [Appl. Opt. 40, 4210 (2001)] and promotes more realistic models.

The origin of diffusive transport of light in dry foams is still under debate. In this paper, we consider the random walks of photons as they are reflected or transmitted by liquid films according to the rules of ray optics. The foams are approximately modeled by three-dimensional Voronoi tessellations with varying degree of disorder. We study two cases: A constant intensity reflectance and the reflectance of thin films. Especially in the second case, we find that in the experimentally important regime for the film thicknesses, the transport-mean-free path l;{ *} does not significantly depend on the topological and geometrical disorder of the Voronoi foams including the periodic Kelvin foam. This may indicate that the detailed structure of foams is not crucial for understanding the diffusive transport of light. Furthermore, our theoretical values for l;{ *} fall in the same range as the experimental values observed in dry foams. One can therefore argue that liquid films contribute substantially to the diffusive transport of light in dry foams.

We study the persistent random walk of photons on a one-dimensional lattice of random transmittances. Transmittances at different sites are assumed independent, distributed according to a given probability density f(t). Depending on the behavior of f(t) near t=0, diffusive and subdiffusive transports are predicted by the disorder expansion of the mean square-displacement and the effective medium approximation. Monte Carlo simulations confirm the anomalous diffusion of photons. To observe photon subdiffusion experimentally, we suggest a dielectric film stack for realization of a distribution f(t).

We construct a stochastic cellular automata model for the description of vehicular traffic at a roundabout designed at the intersection of two perpendicular streets. The vehicular traffic is controlled by a self-organized scheme in which traffic lights are absent. This controlling method incorporates a yield-at-entry strategy for the approaching vehicles to the circulating traffic flow in the roundabout. Vehicular dynamics is simulated and the delay experienced by the traffic at each individual street is evaluated. We discuss the impact of the geometrical properties of the roundabout on the total delay. We compare our results with traffic-light signalization schemes, and obtain the critical traffic volume over which the intersection is optimally controlled through traffic-light signalization schemes.

Sharp debridement is a fast method of achieving a clean leg ulcer, which promotes healing and enables skin grafting. EMLA cream is the only topical anaesthetic for which there is clinical evidence of analgesic efficacy for debridement. Thirteen clinical investigations of EMLA are reviewed. Four double-blind studies and one open randomised controlled study show that EMLA applied to the ulcer for 30-45 min under occlusion significantly reduces the pain from sharp debridement, decreases the incidence of post-debridement pain and reduces the time needed to achieve a clean ulcer, giving potential savings in healthcare costs. Doses of up to 10 g EMLA result in plasma levels of lidocaine and prilocaine well below toxic levels. Repeated treatment does not change the bacterial flora of the ulcer and rarely causes sensitisation. The treatment of pain in leg ulcer patients is important for patient satisfaction and for patient-perceived quality of life.