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Introduction: Sensitive methods are necessary to identify the residual structure in an unfolded protein, which may be similar to the functionally native structure. Signal intensity in NMR experiments is useful for analyzing the line width for a dynamic structure; however, another contribution is contained. Methods: Here, the signal-intensity difference along the sequence was used for probability to calculate the standard deviation. Results: The relative values of the standard deviations were 0.57, 0.57, and 0.66 for alpha-synuclein wild-type, A53T, and A30P, respectively. This revealed that the flexible region was mainly in the Cterminal region of alpha-synuclein at higher temperatures as observed by the amide-proton exchange studies. Conclusion: In particular, the flexible structure was induced by the A30P mutation.

Indirect choroidal rupture due to blunt ocular trauma involves rupture of the choriocapillaris or the full thickness of the choroid and Bruch's membrane. The overlying retinal pigment epithelium and the sensory retina are usually intact or atrophic, but rarely ruptured. We report a case of choroidal rupture healed with proliferative tissue protruding through the sensory retina into the vitreous cavity.A 22-year-old man was punched in the left eye. Hypotony maculopathy and choroidal rupture passing through the macula were noted.The intraocular pressure was normalized after suturing of the dialyzed ciliary body to the sclera and hypotony maculopathy was cured. Proliferative tissue formation protruding through the sensory retina into the vitreous cavity was observed 1 month after the injury. Corrected visual acuity was improved from 0.09 to 0.6. Optical coherence tomography showed that the site of the proliferative tissue was located immediately temporal to the fovea and that the fovea kept its normal form.Optical coherence tomography was useful in monitoring the morphological change in the macula and predicting visual acuity in a case of blunt ocular trauma involving the macular area.