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Your search keyword '"Sagnella, Sharon M."' showing total 39 results
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39 results on '"Sagnella, Sharon M."'

7. Cyto-Immuno-Therapy for Cancer: A Pathway Elicited by Tumor-Targeted, Cytotoxic Drug-Packaged Bacterially Derived Nanocells

Author

Sagnella, Sharon M., primary, Yang, Lu, additional, Stubbs, Gemma E., additional, Boslem, Ebru, additional, Martino-Echarri, Estefania, additional, Smolarczyk, Katarzyna, additional, Pattison, Stacey L., additional, Vanegas, Natasha, additional, St. Clair, Eva, additional, Clarke, Stephen, additional, Boockvar, John, additional, MacDiarmid, Jennifer A., additional, and Brahmbhatt, Himanshu, additional

Published
2020
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9. Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

Author

Tjandra, Kristel C., primary, McCarthy, Nigel, additional, Yang, Lu, additional, Laos, Alistair J., additional, Sharbeen, George, additional, Phillips, Phoebe A., additional, Forgham, Helen, additional, Sagnella, Sharon M., additional, Whan, Renee M., additional, Kavallaris, Maria, additional, Thordarson, Pall, additional, and McCarroll, Joshua A., additional

Published
2019
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11. Targeted Doxorubicin-Loaded Bacterially Derived Nano-Cells for the Treatment of Neuroblastoma

Author

Sagnella, Sharon M., primary, Trieu, Jennifer, additional, Brahmbhatt, Himanshu, additional, MacDiarmid, Jennifer A., additional, MacMillan, Alex, additional, Whan, Renee M., additional, Fife, Christopher M., additional, McCarroll, Joshua A., additional, Gifford, Andrew J., additional, Ziegler, David S., additional, and Kavallaris, Maria, additional

Published
2018
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12. Endogenous nonionic saturated monoethanolamide lipids: solid state, lyotropic liquid crystalline, and solid lipid nanoparticle dispersion behavior

Author

Sagnella, Sharon M., Conn, Charlotte E., Krodkiewska, Irena, Moghaddam, Minoo, and Drummond, Calum J.

Subjects
Amides -- Chemical properties, Drug delivery systems -- Analysis, Lipids -- Chemical properties, Liquid crystals -- Chemical properties, Liquid crystals -- Structure, Drugs -- Vehicles, Drugs -- Analysis, Chemicals, plastics and rubber industries
Published
2010

14. Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

Author

Tjandra, Kristel C., McCarthy, Nigel, Yang, Lu, Laos, Alistair J., Sharbeen, George, Phillips, Phoebe A., Forgham, Helen, Sagnella, Sharon M., Whan, Renee M., Kavallaris, Maria, Thordarson, Pall, and McCarroll, Joshua A.

Abstract

Medulloblastoma is a malignant brain tumor diagnosed in children. Chemotherapy has improved survival rates to approximately 70%; however, children are often left with long-term treatment side effects. New therapies that maintain a high cure rate while reducing off-target toxicity are required. We describe for the first time the use of a bacteriophage-peptide display library to identify heptapeptides that bind to medulloblastoma cells. Two heptapeptides that demonstrated high [E1-3 (1)] or low [E1-7 (2)] medulloblastoma cell binding affinity were synthesized. The potential of the peptides to deliver a therapeutic drug to medulloblastoma cells with specificity was investigated by conjugating E1-3 (1) or E1-7 (2) to doxorubicin (5). Both peptide–drug conjugates were cytotoxic to medulloblastoma cells. E1-3 doxorubicin (3) could permeabilize an in vitro blood–brain barrier and showed a marked reduction in cytotoxicity compared to free doxorubicin (5) in nontumor cells. This study provides proof-of-concept for developing peptide–drug conjugates to inhibit medulloblastoma cell growth while minimizing off-target toxicity.

Published
2020
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15. A Rationally Optimized Nanoparticle System for the Delivery of RNA Interference Therapeutics into Pancreatic Tumors in Vivo

Author

Teo, Joann, primary, McCarroll, Joshua A., additional, Boyer, Cyrille, additional, Youkhana, Janet, additional, Sagnella, Sharon M., additional, Duong, Hien T. T., additional, Liu, Jie, additional, Sharbeen, George, additional, Goldstein, David, additional, Davis, Thomas P., additional, Kavallaris, Maria, additional, and Phillips, Phoebe A., additional

Published
2016
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16. Therapeutic targeting of polo-like kinase 1 using RNA-interfering nanoparticles (iNOPs) for the treatment of non-small cell lung cancer

Author

McCarroll, Joshua A., primary, Dwarte, Tanya, additional, Baigude, Huricha, additional, Dang, Jason, additional, Yang, Lu, additional, Erlich, Rafael B., additional, Kimpton, Kathleen, additional, Teo, Joann, additional, Sagnella, Sharon M., additional, Akerfeldt, Mia C., additional, Liu, Jie, additional, Phillips, Phoebe A., additional, Rana, Tariq M., additional, and Kavallaris, Maria, additional

Published
2015
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18. Choice of Capping Group in Tripeptide Hydrogels Influences Viability in the Three-Dimensional Cell Culture of Tumor Spheroids.

Author

Wojciechowski, Jonathan P., Martin, Adam D., Mason, Alexander F., Fife, Christopher M., Sagnella, Sharon M., Kavallaris, Maria, and Thordarson, Pall

Subjects
PHENOTHIAZINE, HYDROGELS, TUMORS, CELL culture, PEPTIDES
Abstract

Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety. [ABSTRACT FROM AUTHOR]

Published
2017
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26. Nanostructured self-assembly materials from neat and aqueous solutions of C18 lipid pro-drug analogues of Capecitabine—a chemotherapy agent. Focus on nanoparticulate cubosomes™ of the oleyl analogue

Author

Gong, Xiaojuan, primary, Moghaddam, Minoo J., additional, Sagnella, Sharon M., additional, Conn, Charlotte E., additional, Mulet, Xavier, additional, Danon, Stephen J., additional, Waddington, Lynne J., additional, and Drummond, Calum J., additional

Published
2011
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36. Stimuli-responsive functionalized mesoporous silica nanoparticles for drug release in response to various biological stimuliElectronic supplementary information (ESI) available: Supplementary figures associated with this article can be found in the online version. See DOI: 10.1039/c3bm60148j

Author

Chen, Xin, Cheng, Xiaoyu, Soeriyadi, Alexander H., Sagnella, Sharon M., Lu, Xun, Scott, Jason A., Lowe, Stuart B., Kavallaris, Maria, and Gooding, J. Justin

Abstract

A silica-based mesoporous nanosphere (MSN) controlled-release drug delivery system has been synthesized and characterized. The system uses l-cysteine derivatized gold nanoparticles (AuNPs), bound to the MSNs using Cu2+as a bridging ion. The AuNPs serve as removable caps that hinder the release of drug molecules inside the amino functionalized MSN mesoporous framework. The modified MSNs themselves exhibit negligible cytotoxicity to living cells, as revealed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The drug delivery system requires one of two biological stimuli to trigger drug release. These stimuli are either: low pH (pH < 5); or elevated levels of adenosine triphosphate (ATP) (concentration > 4 mM). The feasibility of biologically controlled release was demonstrated through the stimuli-induced removal of the AuNP caps over the MSN releasing the anticancer drug doxorubicin. We envisage that this MSN system could play a significant role in developing new generations of controlled-release delivery vehicles.

Published
2013
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37. Dextran-based doxorubicin nanocarriers with improved tumor penetration.

Author

Sagnella SM, Duong H, MacMillan A, Boyer C, Whan R, McCarroll JA, Davis TP, and Kavallaris M

Subjects
Antineoplastic Agents metabolism, Cell Line, Tumor, Cell Survival drug effects, Cell Survival physiology, Dextrans metabolism, Doxorubicin metabolism, Drug Carriers metabolism, Endocytosis physiology, Humans, Antineoplastic Agents administration & dosage, Dextrans administration & dosage, Doxorubicin administration & dosage, Drug Carriers administration & dosage, Endocytosis drug effects
Abstract

Drug delivery systems with improved tumor penetration are valuable assets as anticancer agents. A dextran-based nanocarrier system with aldehyde functionalities capable of forming an acid labile linkage with the chemotherapy drug doxorubicin was developed. Aldehyde dextran nanocarriers (ald-dex-dox) demonstrated efficacy as delivery vehicles with an IC50 of ∼300 nM against two-dimensional (2D) SK-N-BE(2) monolayers. Confocal imaging showed that the ald-dex-dox nanocarriers were rapidly internalized by SK-N-BE(2) cells. Fluorescence lifetime imaging microscopy (FLIM) analysis indicated that ald-dex-dox particles were internalized as intact complexes with the majority of the doxorubicin released from the particle four hours post uptake. Accumulation of the ald-dex-dox particles was significantly enhanced by ∼30% in the absence of glucose indicating a role for glucose and its receptors in their endocytosis. However, inhibition of clathrin dependent and independent endocytosis and macropinocytosis as well as membrane cholesterol depletion had no effect on ald-dex-dox particle accumulation. In three-dimensional (3D) SK-N-BE(2) tumor spheroids, which more closely resemble a solid tumor, the ald-dex-dox nanoparticles showed a significant improvement in efficacy over free doxorubicin, as evidenced by decreased spheroid outgrowth. Drug penetration studies in 3D demonstrated the ability of the ald-dex-dox nanocarriers to fully penetrate into a SK-N-BE(2) tumor spheroids, while doxorubicin only penetrates to a maximum distance of 50 μM. The ald-dex-dox nanocarriers represent a promising therapeutic delivery system for the treatment of solid tumors due to their unique enhanced penetration ability combined with their improved efficacy over the parent drug in 3D.

Published
2014
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38. Lyotropic liquid crystalline self-assembly material behavior and nanoparticulate dispersions of a phytanyl pro-drug analogue of capecitabine-a chemotherapy agent.

Author

Gong X, Moghaddam MJ, Sagnella SM, Conn CE, Danon SJ, Waddington LJ, and Drummond CJ

Subjects
Animals, Antineoplastic Agents adverse effects, Antineoplastic Agents chemistry, Breast Neoplasms drug therapy, Capecitabine, Cell Survival, Deoxycytidine administration & dosage, Deoxycytidine adverse effects, Deoxycytidine chemistry, Deoxycytidine metabolism, Drug Stability, Female, Fluorouracil administration & dosage, Fluorouracil adverse effects, Fluorouracil chemistry, Fluorouracil metabolism, Inhibitory Concentration 50, Mice, Phase Transition, Prodrugs adverse effects, Prodrugs chemistry, Treatment Outcome, Antineoplastic Agents administration & dosage, Antineoplastic Agents metabolism, Deoxycytidine analogs & derivatives, Fluorouracil analogs & derivatives, Liquid Crystals chemistry, Prodrugs administration & dosage, Prodrugs metabolism
Abstract

An amphiphile prodrug, 5'-deoxy-5-fluoro-N⁴-(phytanyloxycarbonyl) cytidine (5-FCPhy) has been prepared and investigated for its self-assembly material properties, in vitro cytotoxicity, and in vivo efficacy as a chemotherapy agent. The phase transitions and stability of the neat amphiphile were characterized by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). X-ray diffraction (XRD) was used to confirm the structure of the neat amphiphile, which was an amorphous glassy material. The lyotropic liquid crystalline self-assembly behavior of the amphiphile prodrug in water was examined by cross polarizing optical microscopy (POM) and small-angle X-ray scattering (SAXS). Under excess water conditions at room temperature, the amphiphile prodrug self-assembles into lyotropic liquid crystalline mesophases of inverse bicontinuous cubic symmetry. Upon aging, the inverse cubic phase slowly transformed to an inverse hexagonal phase. This amphiphile was successfully dispersed into nanoparticles of cubic and hexagonal symmetry. The in vitro cytotoxicity of dispersed nanoparticles was evaluated in seven different normal and cancer cell types and exhibited IC₅₀ values between 70 and 90 μM for all cell types. Evaluation of 5-FCPhy in vivo against a mouse 4T1 breast tumor model displayed a trend of increasing efficacy with increasing dose. Furthermore, after 21 days, tumor volumes in the 0.5 mmol 5-FCPhy treatment group were significantly smaller than all other treatment groups including mice receiving a short chain water-soluble analogue, Capecitabine (a commercially available oral chemotherapy agent), delivered at the same dosage.

Published
2011
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39. Ordered nanostructured amphiphile self-assembly materials from endogenous nonionic unsaturated monoethanolamide lipids in water.

Author

Sagnella SM, Conn CE, Krodkiewska I, Moghaddam M, Seddon JM, and Drummond CJ

Abstract

The self-assembly, solid state and lyotropic liquid crystalline phase behavior of a series of endogenous n-acylethanolamides (NAEs) with differing degrees of unsaturation, viz., oleoyl monoethanolamide, linoleoyl monoethanolamide, and linolenoyl monoethanolamide, have been examined. The studied molecules are known to possess inherent biological function. Both the monoethanolamide headgroup and the unsaturated hydrophobe are found to be important in dictating the self-assembly behavior of these molecules. In addition, all three molecules form lyotropic liquid crystalline phases in water, including the inverse bicontinuous cubic diamond (Q(II)(D)) and gyroid (Q(II)(G)) phases. The ability of the NAE's to form inverse cubic phases and to be dispersed into ordered nanostructured colloidal particles, cubosomes, in excess water, combined with their endogenous nature and natural medicinal properties, makes this new class of soft mesoporous amphiphile self-assembly materials suitable candidates for investigation in a variety of advanced multifunctional applications, including encapsulation and controlled release of therapeutic agents and incorporation of medical imaging agents.

Published
2010
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