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Your search keyword '"Sagulenko, Evgeniy"' showing total 4 results
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4 results on '"Sagulenko, Evgeniy"'

1. Role of Agrobacterium VirB11 ATPase in T-pilus assembly and substrate selection

Author

Sagulenko, Evgeniy, Sagulenko, Vitaliya, Chen, Jun, and Christie, Peter J.

Subjects
Bacteriology -- Research, Bacteria -- Genetic aspects, Adenosine triphosphatase -- Physiological aspects, Gene mutations -- Physiological aspects, Proteins -- Genetic aspects, Biological sciences
Abstract

Research has been conducted on the VirB11 ATPase of Agrobacterium tumefaciens. The effects of the virB11 mutations on the VirB protein accumulations, production of the T-pilus and translocation of the substrates have been examined and described.

Published
2001

2. Activities of virE1 and the VirE1 secretion chaperone in export of the multifunctional VirE2 effector via an Agrobacterium type IV secretion pathway

Author

Zhao, Zhenming, Sagulenko, Evgeniy, Ding, Zhiyong, and Christie, Peter J.

Subjects
Agrobacterium tumefaciens -- Research, Secretion -- Regulation, Genetic regulation -- Analysis, Biological transport -- Genetic aspects, Biological sciences
Abstract

Research discusses the role of virE1 gene and its encoded product VirE1 secretion chaperone in mediating multifunctional effector protein VirE2 export via type IV secretion system in Agrobacterium tumefaciens. Data indicate that virE1 regulates virE2 translation, VirE1 protein stabilizes VirE2, and VirE1 forms a VirE1-VirE2 complex.

Published
2001

3. VirB7 lipoprotein is exocellular and associates with the agrobacterium tumefaciens T pilus

Author

Sagulenko, Vitaliya, Sagulenko, Evgeniy, Jakubowski, Simon, Spudich, Elena, and Christie, Peter J.

Subjects
Agrobacterium tumefaciens -- Research, Lipoproteins -- Physiological aspects, Bacterial proteins -- Physiological aspects, Proteins -- Crosslinking, Biological sciences
Abstract

Results indicate that monomer and homodimer of the outer membrane lipoprotein VirB7 copurify with the T pilus and are involved in the biogenesis of T pilus. Data further reveal that intermolecular cross-linking of pilin monomers is not essential for T pilus biogenesis, but is required for T pilus stabilization.

Published
2001

4. Activities of virE1 and the VirE1 Secretion Chaperone in Export of the Multifunctional VirE2...

Author

Zhenming Zhao and Sagulenko, Evgeniy

Subjects
*MOLECULAR chaperones, *BIOLOGICAL transport
Abstract

Examines the function of virE1 gene and its product, VirE1 secretion chaperone in mediating export VirE2 effector. Effects of cis and trans expression of virE1 on virE2 expression; Self-association of VirE1 in vivo; Mediation of VirE1 stabilization by an interaction with the N terminus of VirE2.

Published
2001
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