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711 results on '"Saidijam, Massoud"'

8. Utilizing an In-silico Approach to Pinpoint Potential Biomarkers for Enhanced Early Detection of Colorectal Cancer.

Author

Gharebaghi, Alireza, Afshar, Saeid, Tapak, Leili, Ranjbar, Hossein, Saidijam, Massoud, and Dinu, Irina

Subjects
ARTIFICIAL neural networks, EARLY detection of cancer, GENE expression, COLORECTAL cancer, DATABASES
Abstract

Objectives: Colorectal cancer (CRC) is a prevalent disease characterized by significant dysregulation of gene expression. Non-invasive tests that utilize microRNAs (miRNAs) have shown promise for early CRC detection. This study aims to determine the association between miRNAs and key genes in CRC. Methods: Two datasets (GSE106817 and GSE23878) were extracted from the NCBI Gene Expression Omnibus database. Penalized logistic regression (PLR) and artificial neural networks (ANN) were used to identify relevant miRNAs and evaluate the classification accuracy of the selected miRNAs. The findings were validated through bipartite miRNA-mRNA interactions. Results: Our analysis identified 3 miRNAs: miR-1228, miR-6765-5p, and miR-6787-5p, achieving a total accuracy of over 90%. Based on the results of the mRNA-miRNA interaction network, CDK1 and MAD2L1 were identified as target genes of miR-6787-5p. Conclusions: Our results suggest that the identified miRNAs and target genes could serve as non-invasive biomarkers for diagnosing colorectal cancer, pending laboratory confirmation. [ABSTRACT FROM AUTHOR]

Published
2024
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38. Effects of Ce[O.sub.2] nanoparticles on the HO-1, NQO1, and GCLC expression in the testes of diabetic rats

Author

Hasanvand, Davood, Amiri, Iraj, Asl, Sara Soleimani, Saidijam, Massoud, Shabab, Nooshin, and Artimani, Tayebe

Subjects
Nanoparticles -- Health aspects, Testis -- Genetic aspects -- Health aspects, Cerium -- Health aspects, Gene expression -- Health aspects, Diabetics -- Genetic aspects, Biological sciences
Abstract

Ce[O.sub.2] nanoparticles (CNPs) as effective ROS scavengers exhibit potent antioxidant activity. In this study the effect of CNPs investigated was on HO-1, NQO1, and GCLC expression in the streptozotocin (STZ)-induced diabetic rats. Twenty-four male Wistar rats were divided into 4 groups: controls did not receive any treatment; diabetic rats received STZ (60 mg/kg daily); CNPs group received CNPs 30 mg/kg daily for 2 weeks; and rats in STZ + CNPs group received CNPs 30 mg/kg daily for 2 weeks following STZ injection. Oxidative stress was evaluated by measurement of total antioxidant capacity (TAC) and total oxidative status (TOS levels). HO-1, NQO1, and GCLC expression was measured using quantitative real-time PCR. Following STZ injection, significant lower levels of TAC and higher levels of TOS were observed. CNPs could alleviate deleterious effects of diabetes through the enhancement of TAC levels and a significant decline in TOS levels. HO-1, NQO1, and GCLC expression in the diabetic rats were lower than controls. HO-1, NQO1, and GCLC was upregulated in the diabetic rats treated with CNPs. There were significant correlations between NQO1 and GCLC, NQO1 and HO-1, and between HO-1 and GCLC expression. Moreover, Nrf2 was associated with NQO1, GCLC, and HO-1 expression. CNPs as Nrf2 upregulator confer protection against oxidative stress in the testes of STZ-induced diabetic rats by upregulating HO-1, GCLC, and NQO1 cytoprotective genes. Key words: CNPs, HO-1, NQO1, GCLC, diabetes mellitus, Nrf2, oxidative stress. Les nanoparticules de Ce[O.sub.2] (NPC) presentent une puissante activite antioxydante en tant que piegeurs efficaces des derives reactifs de l'oxygene. Dans ces travaux, nous avons etudie les effets des NPC sur l'expression des genes HO-1, NQO1 et GCLC dans un modele de diabete provoque par la streptozotocine (STZ) chez le rat. Nous avons reparti 24 rats Wistar males dans 4 groupes : les temoins n'ont rien recu, les rats atteints de diabete ont recu de la STZ (60 mg/kg par jour), les rats du groupe NPC ont recu des NPC a raison de 30 mg/kg par jour pendant 2 semaines et les rats du groupe STZ + NPC ont recu des NPC a raison de 30 mg/kg par jour sur 2 semaines apres l'injection de STZ. Nous avons evalue le stress oxydatif en mesurant les taux de capacite antioxydante totale (TAC) et d'etat antioxydant total (TOS). Nous avons mesure l'expression d'HO-1, de NQO1 et de GCLC a l'aide de la technique de PCR quantitatif en temps reel. A la suite de l'injection de STZ, nous avons observe de nettes diminutions et augmentations des taux de TAC et de TOS, respectivement. Les NPC permettaient d'attenuer les effets deleteres du diabete par l'intermediaire d'augmentations et de nettes diminutions des taux de TAC et de TOS, respectivement. Les taux d'expression d'HO-1, de NQO1 et de GCLC etaient moins eleves chez les rats atteints de diabete que chez les temoins. Chez les rats atteints de diabete, HO-1, NQO1 et GCLC etaient regules a la hausse avec l'administration de NPC. Nous avons observe des correlations entre les expressions de NQO1 et de GCLC, de NQO1 et d'HO-1, ainsi que d'HO-1 et de GCLC. De plus, l'expression de Nrf2 etait associee a l'expression de NQO1, de GCLC et d'HO-1. Chez les rats atteints diabete provoque par la STZ, la regulation a la hausse de Nrf2 par les NPC confere aux testicules une protection contre le stress oxydatif par la regulation a la hausse des genes cytoprotecteurs HO-1, GCLC et NQO1. [Traduit par la Redaction] Mots-cles : nanoparticules de Ce[O.sub.2] (NPC), HO-1, NQO1, GCLC, diabete sucre, Nrf2, stress oxydatif., Introduction Diabetes mellitus (DM) is a group of metabolic disorders characterized by the presence of hyperglycemia that results from impaired insulin function and (or) secretion (American Diabetes Association 2014). DM [...]

Published
2018
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