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1. Fund Black scientists

Author

Stevens, Kelly R, Masters, Kristyn S, Imoukhuede, PI, Haynes, Karmella A, Setton, Lori A, Cosgriff-Hernandez, Elizabeth, Bell, Muyinatu A Lediju, Rangamani, Padmini, Sakiyama-Elbert, Shelly E, Finley, Stacey D, Willits, Rebecca K, Koppes, Abigail N, Chesler, Naomi C, Christman, Karen L, Allen, Josephine B, Wong, Joyce Y, El-Samad, Hana, Desai, Tejal A, and Eniola-Adefeso, Omolola

Subjects
Complementary and Integrative Health, Black or African American, Biomedical Research, Financial Management, Humans, National Institutes of Health (U.S.), Racial Groups, Research Personnel, United States, Biological Sciences, Medical and Health Sciences, Developmental Biology
Abstract

Our nationwide network of BME women faculty collectively argue that racial funding disparity by the National Institutes of Health (NIH) remains the most insidious barrier to success of Black faculty in our profession. We thus refocus attention on this critical barrier and suggest solutions on how it can be dismantled.

Published
2021

7. List of Contributors

Author

Acharya, Abhinav, primary, Ackun-Farmmer, Marian A., additional, Aggas, John R., additional, Andersen, Phillip J., additional, Anderson, James M., additional, Anseth, Kristi, additional, Archer, Paul A., additional, Ashammakhi, Nureddin, additional, Avila, Jose D., additional, Babensee, Julia E., additional, Badylak, Stephen f., additional, Baek, Kiheon, additional, Baker, Aaron B., additional, Bakht, Syeda Mahwish, additional, Bandyopadhyay, Amit, additional, Barchowsky, Aaron, additional, Bass, Garrett, additional, Becker, Matthew L., additional, Belleghem, Sarah Miho Van, additional, Benoit, Danielle S.W., additional, Biesiekierski, Arne, additional, Billiar, Kristen L., additional, Bose, Susmita, additional, Bowman, Christopher, additional, Boyce, Steven, additional, Brown, Bryan N., additional, Brown, Justin L., additional, Capadona, Jeffrey R., additional, Castner, David G., additional, Chang, Calvin, additional, Chang, Philip, additional, Chilkoti, Ashutosh, additional, Christman, Karen L., additional, Chung, Sangwon, additional, Coleman, Kelly P., additional, Conway, Dan, additional, Cook, Keith E., additional, Cooper, Stuart L., additional, Cosgriff-Hernandez, Elizabeth, additional, Coury, Arthur J., additional, Criscione, Joseph D., additional, Culver, Heidi, additional, Curtis, Jim, additional, Deng, Feiyang, additional, Dhavalikar, Prachi, additional, Diaz-Gomez, Luis, additional, Domingues, Rui M.A., additional, Duncan, Elaine, additional, Duran, Pamela, additional, Esbrit, Pedro, additional, Eskin, Suzanne G., additional, Esmail, Michael Y., additional, Ferracane, Jack L., additional, Fischbach, Claudia, additional, Fischman, Gary, additional, Fisher, John P., additional, Francolini, Iolanda, additional, Frey, Steven J., additional, Gaharwar, Akhilesh K., additional, García, Andrés J., additional, Gibson, Iain R., additional, Gilbert, Jeremy L., additional, Ginn, Brian, additional, Goldblatt, Zachary E., additional, Goldenberg, Seth J., additional, Gomes, Manuela E., additional, Gómez-Florit, Manuel, additional, Gonçalves, Inês C., additional, Gorbet, Maud B., additional, Grainger, David W., additional, Grody, Miles, additional, Guda, Teja, additional, Guelcher, Scott A., additional, Guiseppi-Elie, Anthony, additional, Hacking, S. Adam, additional, Hallab, Nadim James, additional, Hall-Stoodley, Luanne, additional, Hanson, Stephen R., additional, Han, Woojin M., additional, Harman, Melinda K., additional, Harrington, Roger, additional, Haschak, Martin J., additional, Heath, Daniel E., additional, Hicks, Emily Anne, additional, Hill, Ryan T., additional, Hoffman, Allan S., additional, Horbett, Thomas A., additional, Hubbell, Jeffrey A., additional, Ipek, Rasim, additional, Jacobs, Joshua J., additional, Jang, Young C., additional, Jiang, Shaoyi, additional, Johnson, Richard J., additional, Jones, Julian R., additional, Jo, Vickie Y., additional, Kane, Ravi S., additional, Kaplan, David L., additional, Kar, Ronit, additional, Keselowsky, Benjamin George, additional, Khademhosseini, Ali, additional, Kim, Yu Seon, additional, King, Martin W., additional, Kohane, Daniel S., additional, Kohn, David H., additional, Kuhn, Liisa T., additional, Kulkarni, Mangesh, additional, Kuo, Catherine K., additional, Lai, Angela, additional, Lambert, Bryron, additional, Lan, Ziyang, additional, Latour, Robert A., additional, Laurencin, Cato T., additional, Lawson, Bryan K., additional, Layland, Shannon Lee, additional, Lee, Jae Sung, additional, Lee-Parritz, David, additional, Lei, Ying, additional, Lemons, Jack E., additional, Levy, Robert J., additional, Lewerenz, Gregory M., additional, Lewis, Jamal S., additional, Liebscher, Simone, additional, Lin, Chien-Chi, additional, Livingston, Natalie K., additional, Li, Yang, additional, Li, Yuncang, additional, Lu, Helen, additional, Macdougall, Laura, additional, Mahadik, Bhushan, additional, Mamidwar, Sachin, additional, Manspeaker, Margaret P., additional, Mao, Hai-Quan, additional, Ma, Peter X., additional, Marcet, Tyler, additional, Martin, Jeffrey, additional, Martins, M. Cristina L., additional, McArthur, Sally L., additional, McGill, Meghan, additional, McIntire, Larry V., additional, Mei, Lei, additional, Mendes, Bárbara B., additional, Mikos, Antonios G., additional, Mitchell, Richard N., additional, Mitra, Indranath, additional, Muirhead, Ben, additional, Munir, Khurram, additional, Murphy, William L., additional, Nguyen, Phong K., additional, Nolfi, Alexis L., additional, Overby, Clyde, additional, Ozan, Sertan, additional, Padera, Robert F., additional, Pearce, Hannah A., additional, Peppas, Nicholas A., additional, Pereira, Andreia T., additional, Pfeifer, Carmem S., additional, Pinto, Artur M., additional, Raia, Nicole R., additional, Rankin, Edward A., additional, Ratner, Buddy D., additional, Regi, Maria Vallet, additional, Reis, Rui L., additional, Ritchie, Alastair Campbell, additional, Sakiyama-Elbert, Shelly E., additional, Salhadar, Karim, additional, Salinas, Antonio J., additional, Schenke-Layland, Katja, additional, Schoen, Frederick J., additional, Schutrum, Brittany E., additional, Sefton, Michael V., additional, Seidman, Michael A., additional, Shah, Darshan S., additional, Sheardown, Heather, additional, Shoffstall, Andrew J., additional, Simon, Carl G., additional, Simon, Josh, additional, Sims Jr., Kenneth R., additional, Slack, Steven M., additional, Slavin, Benjamin, additional, Snodderly, Kirstie Lane, additional, Stayton, Patrick S., additional, Steichen, Stephanie D., additional, Stoodley, Paul, additional, Swanson, W. Benton, additional, Tam, Hobey, additional, Tayab, Aftab, additional, Thomas, Susan N., additional, Traxel, Kellen D., additional, Tuan, Rocky S., additional, Tucker, Erik I., additional, Ukita, Rei, additional, Veith, Austin, additional, Vidal Yucha, Sarah E., additional, Viney, Christopher, additional, Vyavahare, Naren, additional, Wagner, William R., additional, Wang, Min, additional, Wang, Raymond M., additional, Warner, Petra, additional, Wen, Cuie, additional, West, Jennifer L., additional, Whitman, Matthew A., additional, Witte, Frank, additional, Wolf, Michael F., additional, Yao, Zhicheng, additional, Yaszemski, Michael, additional, Yaszemski, Michael J., additional, Yin, Lichen, additional, Zhang, Guigen, additional, Zhang, Peng, additional, Zhong, Zhiyuan, additional, and Ziats, Nicholas P., additional

Published
2020
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10. Preface

Author

Wagner, William R., primary, Sakiyama-Elbert, Shelly E., additional, Zhang, Guigen, additional, Yaszemski, Michael J., additional, Ratner, Buddy D., additional, Hoffman, Allan S., additional, Schoen, Frederick J., additional, and Lemons, Jack E., additional

Published
2020
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11. Introduction to Biomaterials Science

Author

Ratner, Buddy D., primary, Hoffman, Allan S., additional, Schoen, Frederick J., additional, Lemons, Jack E., additional, Wagner, William R., additional, Sakiyama-Elbert, Shelly E., additional, Zhang, Guigen, additional, and Yaszemski, Michael J., additional

Published
2020
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27. Additional file 1 of A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures

Author

Pardieck, Jennifer, Harb, Manwal, and Sakiyama-Elbert, Shelly E.

Abstract

Additional file 1: Fig. S1. Day 11 quantification of Evx1, Lim1, and βIII tubulin by ICC image analysis. V0V IN induction cultures were either unselected or selected with 4 µg/ml puro from day 10 to 11, then fixed and stained on day 11. N = 1 with n = 2 for unselected and n = 5 for selected cultures. Error bars are S.E.M. among technical replicates. Figure S1 is associated with Fig. 3. Additional file 1: Fig. S2. Day 22 quantification of mature neuron and synaptic markers by ICC image analysis. V0V IN induction cultures were selected with 4 µg/ml puro from day 10 to 11 and cultured until day 22, when they were fixed and stained. Percentage of selected cultured cells expressing A VGLUT2, NeuN, and MAP2 or B VAChT, Bassoon, and MAP2 and respective co-staining combinations are shown. N = 2 with n = 3–5 and error bars are S.E.M. Figure S2 is associated with Fig. 4.

Published
2022
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30. Metabolic regulation of intrasynovial flexor tendon repair: The effects of dichloroacetate administration on early tendon healing in a canine model.

Author

Gelberman, Richard H., Lane, Ryan A., Sakiyama‐Elbert, Shelly E., Thomopoulos, Stavros, and Shen, Hua

Subjects
FLEXOR tendons, TENDON injury healing, PYRUVATE dehydrogenase kinase, ORAL drug administration, LACTATES, OXIDATIVE phosphorylation
Abstract

Enriched in glycolytic enzymes, paucicellular and hypovascular intrasynovial flexor tendons fail to mount an effective healing response after injury and repair. In contrast, well‐vascularized extrasynovial flexor tendons possess high levels of oxidative phosphorylation (OXPHOS) enzymes and have a markedly improved healing capacity. This study was designed to compare the metabolic profiles of the two types of tendons and to evaluate the impact of metabolic reprogramming on early intrasynovial tendon healing in a clinically relevant canine model. Results showed that healthy intrasynovial tendons expressed higher levels of PDK1 and GAPDH and lower levels of SCX and IGF1 than did extrasynovial tendons. PDK1 encodes a subtype of pyruvate dehydrogenase kinase (PDK) that inhibits OXPHOS. Consistently, ATP production via glycolysis was favored in intrasynovial tendon cells whereas OXPHOS was the preferred pathway in extrasynovial tendon cells. Inhibition of glycolysis in vitro increased SCX expression in intrasynovial tendon cells. Therefore, dichloroacetate (DCA), a PDK1 inhibitor, was used in vivo to shift intrasynovial tendon ATP production from glycolysis to OXPHOS. Oral DCA administration reduced serum lactate concentration and increased acetyl‐CoA content in repaired intrasynovial tendons and led to reduced TLR4 and IL1B and increased IGF1, SCX, and TGFB3 expressions in treated intrasynovial tendons compared to controls. Immunohistochemistry staining with anti‐Ki67 and anti‐CD31 antibodies revealed marked increases in cellularity and neovascularization in treated intrasynovial tendons. Clinical significance: The findings of this experiment indicate that improved gene expression and histological outcomes can be achieved by regulating glucose metabolism in the early stages following intrasynovial tendon repair. [ABSTRACT FROM AUTHOR]

Published
2023
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33. Passive Clearing and 3D Lightsheet Imaging of the Intact and Injured Spinal Cord in Mice

Author

McCreedy, Dylan A., primary, Jalufka, Frank L., additional, Platt, Madison E., additional, Min, Sun Won, additional, Kirchhoff, Megan A., additional, Pritchard, Anna L., additional, Reid, Shelby K., additional, Manlapaz, Ronald, additional, Mihaly, Eszter, additional, Butts, Jessica C., additional, Iyer, Nisha R., additional, Sakiyama-Elbert, Shelly E., additional, Crone, Steven A., additional, and McDevitt, Todd C., additional

Published
2021
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39. Flexor Tendon Injury and Repair

Author

Shen, Hua, primary, Yoneda, Susumu, additional, Sakiyama-Elbert, Shelly E., additional, Zhang, Qiang, additional, Thomopoulos, Stavros, additional, and Gelberman, Richard H., additional

Published
2021
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42. A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures.

Author

Pardieck, Jennifer, Harb, Manwal, and Sakiyama-Elbert, Shelly E.

Subjects
EMBRYONIC stem cells, INTERNEURONS, NEURAL circuitry, TRANSGENIC mice, PUROMYCIN, CELL lines, CENTRAL nervous system
Abstract

Background: Spinal interneurons (INs) relay sensory and motor control information between the brain and body. When this relay circuitry is disrupted from injury or disease, it is devastating to patients due to the lack of native recovery in central nervous system (CNS) tissues. Obtaining a purified population of INs is necessary to better understand their role in normal function and as potential therapies in CNS. The ventral V0 (V0V) INs are excitatory neurons involved in locomotor circuits and are thus of interest for understanding normal and pathological spinal cord function. To achieve scalable amounts of V0V INs, they can be derived from pluripotent sources, such as mouse embryonic stem cells (mESCs), but the resultant culture is heterogenous, obscuring the specific role of V0V INs. This study generated a transgenic mESC line to enrich V0V INs from induced cultures to allow for a scalable, enriched population for future in vitro and in vivo studies. Methods: The transgenic Evx1-PAC mESC line was created by CRISPR-Cas9-mediated insertion of puromycin-N-acetyltransferase (PAC) into the locus of V0V IN marker Evx1. Evx1 and PAC mRNA expression were measured by qPCR. Viability staining helped establish the selection protocol for V0V INs derived from Evx1-PAC mESCs inductions. Immunostaining was used to examine composition of selected inductions. Cultures were maintained up to 30 days to examine maturation by expression of mature/synaptic markers, determined by immunostaining, and functional activity in co-cultures with selected motor neurons (MNs) and V2a INs on microelectrode arrays (MEAs). Results: V0V IN inductions were best selected with 4 µg/mL puromycin on day 10 to 11 and showed reduction of other IN populations and elimination of proliferative cells. Long-term selected cultures were highly neuronal, expressing neuronal nuclear marker NeuN, dendritic marker MAP2, pre-synaptic marker Bassoon, and glutamatergic marker VGLUT2, with some cholinergic VAChT-expressing cells. Functional studies on MEAs showed that co-cultures with MNs or MNs plus V2a INs created neuronal networks with synchronized bursting. Conclusions: Evx1-PAC mESCs can be used to purify V0V IN cultures for largely glutamatergic neurons that can be used in network formation studies or for rodent models requiring transplanted V0V INs. [ABSTRACT FROM AUTHOR]

Published
2022
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46. Effect of Connective Tissue Growth Factor Delivered Via Porous Sutures on the Proliferative Stage of Intrasynovial Tendon Repair

Author

Linderman, Stephen W., Shen, Hua, Yoneda, Susumu, Jayaram, Rohith, Tanes, Michael L., Sakiyama-Elbert, Shelly E., Xia, Younan, Thomopoulos, Stavros, and Gelberman, Richard H.

Subjects
Inflammation, Proteomics, Principal Component Analysis, Wound Healing, Sutures, Macrophages, Connective Tissue Growth Factor, bcl-X Protein, Antigens, Differentiation, Myelomonocytic, Receptors, Cell Surface, Article, Biomechanical Phenomena, Tendons, Dogs, Microscopy, Electron, Transmission, Antigens, CD, Tendon Injuries, Tensile Strength, Animals, Intercellular Signaling Peptides and Proteins, Female, Collagen, Stress, Mechanical, Porosity, Cell Proliferation
Abstract

Recent growth factor, cell, and scaffold-based experimental interventions for intrasynovial flexor tendon repair have demonstrated therapeutic potential in rodent models. However, these approaches have not achieved consistent functional improvements in large animal trials due to deleterious inflammatory reactions to delivery materials and insufficient induction of targeted biological healing responses. In this study, we achieved porous suture-based sustained delivery of connective tissue growth factor (CTGF) into flexor tendons in a clinically relevant canine model. Repairs with CTGF-laden sutures were mechanically competent and did not show any evidence of adhesions or other negative inflammatory reactions based on histology, gene expression, or proteomics analyses at 14 days following repair. CTGF-laden sutures induced local cellular infiltration and a significant biological response immediately adjacent to the suture, including histological signs of angiogenesis and collagen deposition. There were no evident widespread biological effects throughout the tendon substance. There were significant differences in gene expression of the macrophage marker CD163 and anti-apoptotic factor BCL2L1; however, these differences were not corroborated by proteomics analysis. In summary, this study provided encouraging evidence of sustained delivery of biologically active CTGF from porous sutures without signs of a negative inflammatory reaction. With the development of a safe and effective method for generating a positive local biological response, future studies can explore additional methods for enhancing intrasynovial tendon repair. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2052-2063, 2018.

Published
2018

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