Search

Your search keyword '"Saldaña, Azael"' showing total 295 results
Start Over Author "Saldaña, Azael"
Sorry, I don't understand your search. ×
295 results on '"Saldaña, Azael"'

5. Epidemiology and Genetic Characterization of Leishmania RNA Virus in Leishmania (Viannia) spp. Isolates from Cutaneous Leishmaniasis Endemic Areas in Panama.

Author

Bonilla, Armando Assair, Pineda, Vanessa, Calzada, José Eduardo, Saldaña, Azael, Laurenti, Marcia Dalastra, Goya, Stephanie, Abrego, Leyda, and González, Kadir

Subjects
CUTANEOUS leishmaniasis, DOUBLE-stranded RNA, GENETIC epidemiology, GENETIC variation, LEISHMANIASIS, RNA viruses
Abstract

Leishmania (Viannia) spp. can harbor a double-stranded RNA virus known as Leishmania RNA virus 1 (LRV-1), whose presence has been reported in nine countries across the Americas and seven Leishmania species. Here, we studied 100 Leishmania (Viannia) isolates from patients with cutaneous leishmaniasis collected from different endemic areas in Panama from 2016 to 2022. We identified L. (V.) panamensis, L. (V.) guyanensis, L. (V.) braziliensis/guyanensis hybrid, and L. (V.) panamensis sp.1. (genetic variant). LRV-1 was detected by RT-PCR in 9% of L. (Viannia) isolates (eight cases in L. (V.) panamensis, and one in L. (V.) guyanensis). Phylogenetic analysis based on sequencing data classified all LRV-1 isolates within genotype A, suggesting that LRV phylogenetic proximity is closely aligned with geographical distribution or to the phylogenetic proximity of the Leishmania host in the case of the L. (V.) panamensis and L. (V.) guyanensis in Panama. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

12. Detection of Leishmania RNA Virus 1 in Leishmania (Viannia) panamensis Isolates, Panama

Author

Gonzalez, Kadir, primary, De León, Santiago S., additional, Pineda, Vanessa, additional, Samudio, Franklyn, additional, Capitan-Barrios, Zeuz, additional, Suarez, José Antonio, additional, Weeden, Adriana, additional, Ortiz, Betsi, additional, Rios, Margarita, additional, Moreno, Brechla, additional, Gundacker, Nathan D., additional, Pascale, Juan M., additional, López-Vergès, Sandra, additional, Sosa, Néstor, additional, Saldaña, Azael, additional, and Ábrego, Leyda E., additional

Published
2023
Full Text
View/download PDF

17. On palms, bugs, and Chagas disease in the Americas

Author

Abad-Franch, Fernando, Lima, Marli M., Sarquis, Otília, Gurgel-Gonçalves, Rodrigo, Sánchez-Martín, María, Calzada, José, Saldaña, Azael, Monteiro, Fernando A., Palomeque, Francisco S., Santos, Walter S., Angulo, Victor M., Esteban, Lyda, Dias, Fernando B.S., Diotaiuti, Liléia, Bar, María Esther, and Gottdenker, Nicole L.

Published
2015
Full Text
View/download PDF

26. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp

Author

Sandoval Pacheco, Carmen M., primary, Araujo Flores, Gabriela V., additional, Gonzalez, Kadir, additional, de Castro Gomes, Claudia M., additional, Passero, Luiz F. D., additional, Tomokane, Thaise Y., additional, Sosa-Ochoa, Wilfredo, additional, Zúniga, Concepción, additional, Calzada, Jose, additional, Saldaña, Azael, additional, Corbett, Carlos E. P., additional, Silveira, Fernando T., additional, and Laurenti, Marcia D., additional

Published
2021
Full Text
View/download PDF

28. Molecular Identification of Parasites Causing Cutaneous Leishmaniasis in Panama

Author

Miranda, Aracelis del C., primary, González, Kadir A., additional, Samudio, Franklyn, additional, Pineda, Vanessa J., additional, Calzada, José E., additional, Capitan-Barrios, Zeuz, additional, Jiménez, Ana, additional, Castillo, Juan, additional, Mendoza, Yaxelis, additional, Suárez, José A., additional, Ortiz, Betsi, additional, Méndez, Juan, additional, Pascale, Juan M., additional, Grögl, Max, additional, Sosa, Néstor, additional, and Saldaña, Azael, additional

Published
2021
Full Text
View/download PDF

29. Repeat-Driven Generation of Antigenic Diversity in a Major Human Pathogen, Trypanosoma cruzi

Author

Talavera-López, Carlos, primary, Messenger, Louisa A., additional, Lewis, Michael D., additional, Yeo, Matthew, additional, Reis-Cunha, João Luís, additional, Matos, Gabriel Machado, additional, Bartholomeu, Daniella C., additional, Calzada, José E., additional, Saldaña, Azael, additional, Ramírez, Juan David, additional, Guhl, Felipe, additional, Ocaña-Mayorga, Sofía, additional, Costales, Jaime A., additional, Gorchakov, Rodion, additional, Jones, Kathryn, additional, Nolan, Melissa S., additional, Teixeira, Santuza M. R., additional, Carrasco, Hernán José, additional, Bottazzi, Maria Elena, additional, Hotez, Peter J., additional, Murray, Kristy O., additional, Grijalva, Mario J., additional, Burleigh, Barbara, additional, Grisard, Edmundo C., additional, Miles, Michael A., additional, and Andersson, Björn, additional

Published
2021
Full Text
View/download PDF

35. In situ study of cellular immune response in human cutaneous lesions caused by Leishmania (Viannia) panamensis in Panama

Author

Gonzalez, Kadir, primary, Calzada, José Eduardo, additional, Tomokane, Thaise Yumie, additional, Pacheco, Carmen Maria Sandoval, additional, Flores, Gabriela Venicia Araujo, additional, Castro Gomes, Cláudia Maria, additional, Pereira Corbett, Carlos Eduardo, additional, Saldaña, Azael, additional, and Laurenti, Marcia Dalastra, additional

Published
2020
Full Text
View/download PDF

37. Long-term transmission patterns and public health policies leading to malaria elimination in Panamá

Author

Hurtado, Lisbeth, primary, Cumbrera, Alberto, additional, Rigg, Chystrie, additional, Perea, Milixa, additional, Santamaría, Ana María, additional, Chaves, Luis Fernando, additional, Moreno, Dianik, additional, Romero, Luis, additional, Lasso, Jose, additional, Caceres, Lorenzo, additional, Saldaña, Azael, additional, and Calzada, Jose Eduardo, additional

Published
2020
Full Text
View/download PDF

39. Correction: Synanthropic Mammals as Potential Hosts of Tick-Borne Pathogens in Panama

Author

Bermúdez, Sergio E., primary, Gottdenker, Nicole, additional, Krishnavajhala, Aparna, additional, Fox, Amy, additional, Wilder, Hannah K., additional, González, Kadir, additional, Smith, Diorene, additional, López, Marielena, additional, Perea, Milixa, additional, Rigg, Chystrie, additional, Montilla, Santiago, additional, Calzada, José E., additional, Saldaña, Azael, additional, Caballero, Carlos M., additional, and Lopez, Job E., additional

Published
2019
Full Text
View/download PDF

42. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of sp.

Author

Sandoval Pacheco, Carmen M., Araujo Flores, Gabriela V., Gonzalez, Kadir, de Castro Gomes, Claudia M., Passero, Luiz F. D., Tomokane, Thaise Y., Sosa-Ochoa, Wilfredo, Zúniga, Concepción, Calzada, Jose, Saldaña, Azael, Corbett, Carlos E. P., Silveira, Fernando T., and Laurenti, Marcia D.

Subjects
MACROPHAGES, CUTANEOUS leishmaniasis, LEISHMANIA, INTRACELLULAR pathogens, IMMUNE response
Abstract

Macrophages play important roles in the innate and acquired immune responses against Leishmania parasites. Depending on the subset and activation status, macrophages may eliminate intracellular parasites; however, these host cells also can offer a safe environment for Leishmania replication. In this sense, the fate of the parasite may be influenced by the phenotype of the infected macrophage, linked to the subtype of classically activated (M1) or alternatively activated (M2) macrophages. In the present study, M1 and M2 macrophage subsets were analyzed by double-staining immunohistochemistry in skin biopsies from patients with American cutaneous leishmaniasis (ACL) caused by L. (L.) amazonensis, L. (V.) braziliensis, L. (V.) panamensis ,and L. (L.) infantum chagasi. High number of M1 macrophages was detected in nonulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi (M1 = 112 ± 12, M2 = 43 ± 12 cells/mm2). On the other side, high density of M2 macrophages was observed in the skin lesions of patients with anergic diffuse cutaneous leishmaniasis (ADCL) (M1 = 195 ± 25, M2 = 616 ± 114), followed by cases of localized cutaneous leishmaniasis (LCL) caused by L. (L.) amazonensis (M1 = 97 ± 24, M2 = 219 ± 29), L. (V.) panamensis (M1 = 71 ± 14, M2 = 164 ± 14), and L. (V.) braziliensis (M1 = 50 ± 13, M2 = 53 ± 10); however, low density of M2 macrophages was observed in NUCL. The data presented herein show the polarization of macrophages in skin lesions caused by different Leishmania species that may be related with the outcome of the disease. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

43. In situ study of cellular immune response in human cutaneous lesions caused by Leishmania (Viannia) panamensis in Panama.

Author

Gonzalez, Kadir, Calzada, José Eduardo, Tomokane, Thaise Yumie, Pacheco, Carmen Maria Sandoval, Flores, Gabriela Venicia Araujo, Castro Gomes, Cláudia Maria, Pereira Corbett, Carlos Eduardo, Saldaña, Azael, and Laurenti, Marcia Dalastra

Subjects
IMMUNE response, CUTANEOUS leishmaniasis, HISTOLOGICAL techniques, LEISHMANIA, T cells, HISTOCHEMISTRY
Abstract

Aims: Leishmaniasis is considered a disease with multiple clinical/immunopathological characteristics, depending on the immunity of the host and the species of the parasite. In Panama, the most prevalent species that causes localized cutaneous leishmaniasis (LCL) is Leishmania (Viannia) panamensis, and its immune response is poorly studied. Therefore, we evaluated by immunohistochemistry, the in situ immune response during this infection. Methods and Results: Biopsies from Panamanian patients with LCL were collected and processed by histological techniques. Infection by L. (V.) panamensis was demonstrated by isolation in culture and molecular characterization by Hsp70‐RFLP. The in situ immune response was assessed by immunohistochemistry. The immune response was characterized by predominance of T cells, mainly CD8 cells that showed positive correlation with IFN‐γ and Granzyme B. CD4 cells presented positive correlation with both IFN‐γ and IL‐13, pointed by mixed cellular immune response. Regulatory response was characterized by FoxP3 cells, which showed positive correlation to IL‐10 but not with TGF‐β. Conclusions: L. (V.) panamensis infection triggers a mixed cellular immune response, characterized by the presence of pro‐inflammatory, anti‐inflammatory and regulatory elements in the skin lesion of Panamanian patients. These data contribute to a better understanding of the immunopathogenesis of Leishmania Viannia infection in Panama. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

45. Repeat-driven generation of antigenic diversity in a major human pathogen Trypanosoma cruzi

Author

Talavera-López, Carlos, primary, Messenger, Louisa A., additional, Lewis, Michael D., additional, Yeo, Matthew, additional, Reis-Cunha, João Luís, additional, Bartholomeu, Daniella C., additional, Calzada, José E., additional, Saldaña, Azael, additional, Ramírez, Juan David, additional, Guhl, Felipe, additional, Ocaña-Mayorga, Sofía, additional, Costales, Jaime A., additional, Gorchakov, Rodion, additional, Jones, Kathryn, additional, Garcia, Melissa Nolan, additional, Grisard, Edmundo C., additional, Teixeira, Santuza M. R., additional, Carrasco, Hernán, additional, Bottazzi, Maria Elena, additional, Hotez, Peter J., additional, Murray, Kristy O., additional, Grijalva, Mario J., additional, Burleigh, Barbara, additional, Miles, Michael A., additional, and Andersson, Björn, additional

Published
2018
Full Text
View/download PDF

49. Experimental Trypanosoma rangeli infection in a murine model

Author

Horna, Aura E, Saldaña, Azael, Örn, Anders, and Sousa, Octavio E

Subjects
parasitic diseases
Abstract

Trypanosoma rangeli experimental murine infections were peñormed in order to study parasitemias and anti-parasite antibody levels. Three groups of miee were used: a) miee infected with metatrypomastigotes derived from infected bugs; b) miee which received four reinoculations of metatrypomastigotes and c) miee immunosuppressed with cyclophosphamide. The results showed that bloodstream parasites can be found from the first day post inoculation reaching a peak at day 5 or 7 and then start to decline. Parasites disappeared completely from the circulation after 20-25 days. However in the imunosuppressed group, parasites were found in blood up to 45 days post infection. The humoral immune response was monitored using an ELISA test and low levels of specific IgG and IgM immunoglobulins were found. However the IgG titers were lower than the IgM. One could conclude that IgM was the predominant immunoglobulin isotype induced in a T. rangeli experimental infection because the highest titers were observed in the reinoculated group. IgM antibodies a1so showed the most prominent crossreactivities with T. cruzi antigens. Se estudió la parasitemia y la respuesta inmune humoral en ratones experimentalmente infectados con Trypanosoma rangeli . En ratones inmunocompetentes los parásitos sanguineos mostraron una máxima parasitemia a los 5-7 días post-inoculación para luego desaparecer de la circulación a los 20-25 días. Sin embargo en ratones tratados con ciclofosfamida la parasitemia se prolongó hasta los 45 días post-inoculación. Durante este tiempo se detectaron, por medio de una prueba de ELISA, anticuerpos específicos IgG e IgM siendo estos últimos los predominantes y de posible importancia en el control de la infección. De forma similar los anticuerpos IgM mostraron mayor reconocimiento de los antígenos de Trypanosoma cruzi . Esta observación enfatiza la necesidad de considerar las infecciones por T. rangeli en el diagnóstico y patología de la enfermedad de Chagas.

Published
2015
Full Text
View/download PDF

50. Risk factors associated with Trypanosoma cruziexposure in domestic dogs from a rural community in Panama

Author

Saldaña, Azael, Calzada, José E, Pineda, Vanessa, Perea, Milixa, Rigg, Chystrie, González, Kadir, Santamaria, Ana Maria, Gottdenker, Nicole L, and Chaves, Luis F

Subjects
Rhodnius pallescens, Chagas disease, Panama, Trypanosoma cruzi, education, parasitic diseases, food and beverages, Attalea butyracea, Canis familiaris
Abstract

Chagas disease, caused by Trypanosoma cruzi infection, is a zoonosis of humans, wild and domestic mammals, including dogs. In Panama, the main T. cruzi vector is Rhodnius pallescens, a triatomine bug whose main natural habitat is the royal palm, Attalea butyracea. In this paper, we present results from three T. cruzi serological tests (immunochromatographic dipstick, indirect immunofluorescence and ELISA) performed in 51 dogs from 24 houses in Trinidad de Las Minas, western Panama. We found that nine dogs were seropositive (17.6% prevalence). Dogs were 1.6 times more likely to become T. cruziseropositive with each year of age and 11.6 times if royal palms where present in the peridomiciliary area of the dog’s household or its two nearest neighbours. Mouse-baited-adhesive traps were employed to evaluate 12 peridomestic royal palms. All palms were found infested with R. pallescens with an average of 25.50 triatomines captured per palm. Of 35 adult bugs analysed, 88.6% showed protozoa flagellates in their intestinal contents. In addition, dogs were five times more likely to be infected by the presence of an additional domestic animal species in the dog’s peridomiciliary environment. Our results suggest that interventions focused on royal palms might reduce the exposure to T. cruzi infection.

Published
2015

Catalog

Books, media, physical & digital resources
See catalog results