1. Comprehensive evaluation of methods to assess overall and cell-specific immune infiltrates in breast cancer
- Author
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Nederlof, I. (Iris), De Bortoli, D. (Davide), Bareche, Y. (Yacine), Nguyen, B. (Bastien), De Maaker, M. (Michiel), Hooijer, J., Buisseret, L. (Laurence), Kok, M. (Marleen), Smid, M. (Marcel), Eynden, G. van den, Brinkman, A.B. (Arie B.), Hudecek, J. (Jan), Koster, J. (Jan), Sotiriou, C. (Christos), Larsimont, D. (Denis), Martens, J.W.M. (John), Vijver, M.J. (Marc ), Horlings, H.M. (Hugo M.), Salgado, R. (Roberto), Biganzoli, L. (Laura), Desmedt, C. (Christine), Nederlof, I. (Iris), De Bortoli, D. (Davide), Bareche, Y. (Yacine), Nguyen, B. (Bastien), De Maaker, M. (Michiel), Hooijer, J., Buisseret, L. (Laurence), Kok, M. (Marleen), Smid, M. (Marcel), Eynden, G. van den, Brinkman, A.B. (Arie B.), Hudecek, J. (Jan), Koster, J. (Jan), Sotiriou, C. (Christos), Larsimont, D. (Denis), Martens, J.W.M. (John), Vijver, M.J. (Marc ), Horlings, H.M. (Hugo M.), Salgado, R. (Roberto), Biganzoli, L. (Laura), and Desmedt, C. (Christine)
- Abstract
Background: Breast cancer (BC) immune infiltrates play a critical role in tumor progression and response to treatment. Besides stromal tumor infiltrating lymphocytes (sTILs) which have recently reached level 1B evidence as a prognostic marker in triple negative BC, a plethora of methods to assess immune infiltration exists, and it is unclear how these compare to each other and if they can be used interchangeably. Methods: Two experienced pathologists scored sTIL, intra-tumoral TIL (itTIL), and 6 immune cell types (CD3+, CD4+, CD8+, CD20+, CD68+, FOXP3+) in the International Cancer Genomics Consortium breast cancer cohort using hematoxylin and eosin-stained (n = 243) and immunohistochemistry-stained tissue microarrays (n = 254) and whole slides (n = 82). The same traits were evaluated using transcriptomic- and methylomic-based deconvolution methods or signatures. Results: The concordance correlation coefficient (CCC) between pathologists for sTIL was very good (0.84) and for cell-specific immune infiltrates slightly lower (0.63-0.66). Comparison between tissue microarray and whole slide pathology scores revealed systematically higher values in whole slides (ratio 2.60-5.98). The Spearman correlations between microscopic sTIL and transcriptomic- or methylomic-based assessment of immune infiltr
- Published
- 2019
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