Search

Your search keyword '"Salminen, Tiina"' showing total 737 results
Start Over Author "Salminen, Tiina"
737 results on '"Salminen, Tiina"'

9. A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila.

Author

Salminen, Tiina S., Vesala, Laura, Basikhina, Yuliya, Kutzer, Megan, Tuomela, Tea, Lucas, Ryan, Monteith, Katy, Prakash, Arun, Tietz, Tilman, and Vale, Pedro F.

Subjects
*FLIES as carriers of disease, *CELLULAR immunity, *DROSOPHILA melanogaster, *SYMPTOMS, *FRUIT flies, *MITOCHONDRIAL DNA
Abstract

The role of mitochondria in immunity is increasingly recognized, but it is unclear how variation in mitochondrial DNA (mtDNA) contributes to variable infection outcomes. To quantify the effect of mtDNA variation on humoral and cell-mediated innate immune responses, we utilized a panel of fruit fly Drosophila melanogaster cytoplasmic hybrids (cybrids), where unique mtDNAs (mitotypes) were introgressed into a controlled isogenic nuclear background. We observed substantial heterogeneity in infection outcomes within the cybrid panel upon bacterial, viral and parasitoid infections, driven by the mitotype. One of the mitotypes, mtKSA2 protected against bacterial, parasitoid, and to a lesser extent, viral infections. Enhanced survival was not a result of improved bacterial clearance, suggesting mtKSA2 confers increased disease tolerance. Transcriptome sequencing showed that the mtKSA2 mitotype had an upregulation of genes related to mitochondrial respiration and phagocytosis in uninfected flies. Upon infection, mtKSA2 flies exhibited infection type and duration specific transcriptomic changes. Furthermore, uninfected mtKSA2 larvae showed immune activation of hemocytes (immune cells), increased hemocyte numbers and ROS production, and enhanced encapsulation response against parasitoid wasp eggs and larvae. Our results show that mtDNA variation acts as an immunomodulatory factor in both humoral and cell-mediated innate immunity and that specific mitotypes can provide broad protection against infections. Author summary: The strength of immune response and the disease symptoms vary among individuals even when exposed to the same pathogen. Much of this variation is due to the genes directly involved in adaptive and innate immune pathways. In addition, mitochondria are emerging as one of the factors modulating immune responses. Mitochondria are cellular organelles with their own genome (mtDNA) involved in many important processes, including producing ATP, the energy currency of the cells. mtDNA mutations leading to mitochondrial dysfunction are often considered harmful upon exposure to pathogens. Here, we used a fruit fly model where unique mtDNA variants are placed in a controlled nuclear genomic background to find out how mtDNA shapes infection outcomes upon bacterial, viral and parasitoid infections. We found that mtDNA variation alters the efficiency of immune response and describe a mtDNA variant that confers protection against variety of pathogens. This protection was at least partially caused by enhanced cell-mediated innate immunity, including higher numbers of immune cells prior to and during infection. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

15. Exposure to loud noise and risk of vestibular schwannoma : results from the INTERPHONE international case–control study

Author

INTERPHONE Study Group, Deltour, Isabelle, Schlehofer, Brigitte, Massardier-Pilonchéry, Amélie, Schlaefer, Klaus, Armstrong, Bruce, Giles, Graham G, Siemiatycki, Jack, Parent, Marie-Elise, Krewski, Daniel, McBride, Mary, Johansen, Christoffer, Auvinen, Anssi, Salminen, Tiina, Hours, Martine, Montestrucq, Lucile, Blettner, Maria, Berg-Beckhoff, Gabriele, Sadetzki, Siegal, Chetrit, Angela, Lagorio, Susanna, Iavarone, Ivano, Yamaguchi, Naohito, Takebayashi, Toru, Woodward, Alistair, Cook, Angus, Tynes, Tore, Klaeboe, Lars, Feychting, Maria, Lönn, Stefan, Fleming, Sarah, Swerdlow, Anthony J, Schoemaker, Minouk J, Moissonnier, Monika, Kesminiene, Ausrele, Cardis, Elisabeth, and Schüz, Joachim

Published
2019

32. Exogenous Trehalose Improves Growth, Glycogen and Poly-3-Hydroxybutyrate (PHB) Contents in Photoautotrophically Grown Arthrospira platensis under Nitrogen Deprivation.

Author

Mudtham, Nat-Anong, Promariya, Authen, Duangsri, Chanchanok, Maneeruttanarungroj, Cherdsak, Ngamkala, Suchanit, Akrimajirachoote, Nattaphong, Powtongsook, Sorawit, Salminen, Tiina A., and Raksajit, Wuttinun

Subjects
POLY-beta-hydroxybutyrate, TREHALOSE, GLYCOGEN, DISACCHARIDES, BIOPOLYMERS, CHEMICAL structure, NITROGEN
Abstract

Simple Summary: Trehalose is an easily and economically obtainable non-reducing disaccharide, readily taken up by living organisms upon external application. Arthrospira platensis grew photoautotrophically under nitrogen-deficient conditions supplied with the appropriate trehalose, promoting glycogen and PHB production. This is supported by a notable increase in the upregulation of glgA and phaC expression, along with the induction of enzyme activity, including glycogen synthase encoded by glgA and PHA synthase encoded by phaC. The present study offers new insights into the contribution of exogenous trehalose in the regulation of glycogen and PHB production in A. platensis. Glycogen and poly-3-hydroxybutyrate (PHB) are excellent biopolymer products from cyanobacteria. In this study, we demonstrate that nitrogen metabolism is positively influenced by the exogenous application of trehalose (Tre) in Arthrospira platensis under nitrogen-deprived (−N) conditions. Cells were cultivated photoautotrophically for 5 days under −N conditions, with or without the addition of exogenous Tre. The results revealed that biomass and chlorophyll-a content of A. platensis experienced enhancement with the addition of 0.003 M and 0.03 M Tre in the −N medium after one day, indicating relief from growth inhibition caused by nitrogen deprivation. The highest glycogen content (54.09 ± 1.6% (w/w) DW) was observed in cells grown for 2 days under the −N + 0.003 M Tre condition (p < 0.05), while the highest PHB content (15.2 ± 0.2% (w/w) DW) was observed in cells grown for 3 days under the −N + 0.03 M Tre condition (p < 0.05). The RT-PCR analysis showed a significant increase in glgA and phaC transcript levels, representing approximately 1.2- and 1.3-fold increases, respectively, in A. platensis grown under −N + 0.003 M Tre and −N + 0.03 M Tre conditions. This was accompanied by the induction of enzyme activities, including glycogen synthase and PHA synthase with maximal values of 89.15 and 0.68 µmol min−1 mg−1 protein, respectively. The chemical structure identification of glycogen and PHB from A. platensis was confirmed by FTIR and NMR analysis. This research represents the first study examining the performance of trehalose in promoting glycogen and PHB production in cyanobacteria under nitrogen-deprived conditions. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

34. Characterization and Homology Modeling of Catalytically Active Recombinant PhaCAp Protein from Arthrospira platensis

Author

Duangsri, Chanchanok, primary, Salminen, Tiina A., additional, Alix, Marion, additional, Kaewmongkol, Sarawan, additional, Akrimajirachoote, Nattaphong, additional, Khetkorn, Wanthanee, additional, Jittapalapong, Sathaporn, additional, Mäenpää, Pirkko, additional, Incharoensakdi, Aran, additional, and Raksajit, Wuttinun, additional

Published
2023
Full Text
View/download PDF

37. Deciphering tissue-induced Klebsiella pneumoniae lipid A structure

Author

Llobet, Enrique, Martínez-Moliner, Verónica, Moranta, David, Dahlström, Käthe M., Regueiro, Verónica, Tomás, Anna, Cano, Victoria, Pérez-Gutiérrez, Camino, Frank, Christian G., Fernández-Carrasco, Helena, Insua, José Luis, Salminen, Tiina A., Garmendia, Junkal, and Bengoechea, José A.

Published
2015

39. Tailored Approaches in Drug Development and Diagnostics: From Molecular Design to Biological Model Systems

Author

Sahlgren, Cecilia, Meinander, Annika, Zhang, Hongbo, Cheng, Fang, Preis, Maren, Xu, Chunlin, Salminen, Tiina A., Toivola, Diana, Abankwa, Daniel, Rosling, Ari, Karaman, Didem Şen, Salo‐Ahen, Outi M. H., Österbacka, Ronald, Eriksson, John E., Willför, Stefan, Petre, Ion, Peltonen, Jouko, Leino, Reko, Johnson, Mark, Rosenholm, Jessica, and Sandler, Niklas

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results