Search

INTRODUCTION: According to the WHO report 2022, 2.21 million new cases and 1.80 million deaths were reported for lung cancer in the year 2020. Therefore, there is an urgent need to explore novel, safe, and effective therapeutic interventions for lung cancer. METHOD: Hence, in the present study, we explored the anticancer potential of vincamine by using network pharmacology, molecular docking, and in vitro approaches. Network pharmacology studies demonstrated that the most common targets of vincamine are G-protein coupled receptors, cytosolic proteins, and enzymes. Among these targets, two targets, ALK and ERBB2 protein, were common between vincamine and non-small cell lung cancer. RESULT: We discovered a link between these two targets and their companion proteins, as well as cancer-related pathways. In addition, a docking investigation between the ligand for vincamine and two targeted genes revealed a strong affinity toward these targeted proteins. Further, the in vitro study demonstrated that vincamine treatment for 72 h led to dose-dependent (0-500 μM) cytotoxicity on the A549 lung cancer cell line with an IC50 value of 291.7 μΜ. The wound-healing assay showed that vincamine treatment (150 and 300 μM) significantly inhibited cell migration and invasion. Interestingly, acridine orange/ethidium bromide dual staining demonstrated that vincamine treatment induces apoptosis in A549 cells. Additionally, the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay showed an increased level of reactive oxygen species (ROS) after the vincamine treatment, indicating ROS-mediated apoptosis in A549 cells. CONCLUSION: Altogether, based on our findings, we hypothesize that vincamine-induced apoptosis of lung cancer cells via ALK and ERBB2 protein modulation may be an attractive futuristic strategy for managing lung cancer in combination with chemotherapeutic agents to obtain synergistic effects with reduced side effects.

Bacterial microflora which is present in gastrointestinal tract cause various disorders. Probiotics provides beneficial effects in gut related disease. Previously probiotics are used as alternative medicine but now entering in mainstream medicine. Probiotics help in restoring normal bacterial microflora and regulate the gastrointestinal tract functioning through different mechanisms. There are only few evidences available which show potential benefits of probiotics. The efficiency of probiotics used as a single strain or multiple formulation is tested in various diseases such oral health, diabetes, multiple sclerosis, inflammatory bowel disease, necrotising enterocolitis and many more. The purpose of this paper is to focus on diseases related to gastrointestinal tract including inflammatory bowel disease and necrotising enterocolitis based on the literature review available and propose future perspective to reduce the translocation of bacterial microflora in gastrointestinal tract and reducing subsequent inflammation of gut. Keywords were searched in goggle scholar, academic research, PubMed and Embase databases.

The diagnosis of keratitis is based on visual exam, tissue cytology, and standard microbial culturing to determine the type of the infectious pathogen. To prescribe appropriate therapy, it is important to distinguish between bacterial, fungal, and viral keratitis, as the treatments are quite different. Diagnosis of the causative organism has a substantial prognostic importance. Further, timely knowledge of the nature of the pathogen is also critical to adapt therapy in patients unresponsive to empiric treatment options, which occurs in 10% of all cases. Currently, the identification of the nature of the pathogen that causes keratitis is achieved via microbial culture screening, which is laboratory-based, expensive, and time-consuming. The most frequent pathogens that cause the corneal ulcers are P. aeruginosa and S. aureus. Here, we report a microchip for rapid (

SAG University Indore, M.P., India, {"references":["Sharma, D. S. T. \"Tagore's Gitanjali: A Note on Publics of Performance\". IJOHMN (International Journal Online of Humanities), Vol. 4, no. 4, Aug. 2018, p. 10, . < https://www.ijohmn.com/index.php/ijohmn/article/view/56/198 >. Date accessed: 9 January 2019. doi:10.24113/ijohmn.v4i4.56.","Chanda, R. (2001). Selected Sotries of Rabindranath Tagore. https://www.parabaas.com/translation/database/reviews/tagoreshortstories1.html , 2.Date accessed: 19 March 2019.","Mukherjee, R. (2012). RABINDRANATH TAGORE'S GITANJALI: A SOURCE. http://www.abhinavjournal.com/images/Arts_&_Education/Apr12/1.pdf, 1 (4), 9. Date accessed: 22 March 2019.","Musing, A. B. (2013). Book Review: Gitanjali. https://abookwormsmusing.wordpress.com/2013/07/03/book-review-gitanjali-by- rabindranath-tagore/ , 1.Date accessed: 28 March 2019","Sharma, D. N. (2016). Tagore's Spiritual Discourse in Gitanjali. https://ijellh.com/OJS/index.php/OJS/article/view/1332/1302, IV (IV), 5.Date accessed: 22 May 2019"]}