Search

Your search keyword '"Salvi, Prafull"' showing total 172 results
Start Over Author "Salvi, Prafull"
172 results on '"Salvi, Prafull"'

18. PROTEIN L‐ISOASPARTYL METHYLTRANSFERASE protects enolase dysfunction by repairing isoaspartyl‐induced damage and is positively implicated in agronomically important seed traits

Author

Kamble, Nitin Uttam, primary, Ghosh, Shraboni, additional, Petla, Bhanu Prakash, additional, Achary, Rakesh Kumar, additional, Gautam, Shikha, additional, Rao, Venkateswara, additional, Salvi, Prafull, additional, Hazra, Abhijit, additional, Varshney, Vishal, additional, and Majee, Manoj, additional

Published
2024
Full Text
View/download PDF

34. Molecular cloning, expression and Insilco analysis of drought stress inducible MYB transcription factor encoding gene from C4 plant Eleusine coracana

Author

Bhatt, Megha, Salvi , Prafull, Chauhan, Ankita, Joshi, Mihir, Lohani, Pushpa, Bhatt, Megha, Salvi , Prafull, Chauhan, Ankita, Joshi, Mihir, and Lohani, Pushpa

Abstract

Drought is one of the key abiotic stresses that critically influences the crops by restraining their growth and yield potential. Being sessile, plant tackle the detrimental effects of drought stress by modulating the cellular state by changing the gene expression. The transcriptional syndicate essentially drives such alteration of gene expression. Transcription factors (TF) are the key regulatory protein that controls the expression of their target gene by binding to the cis-regulatory elements present in the promoter region. Myb-TF, ubiquitously present in all eukaryotes belong to one of the largest TF family, and play a wide array of biological functions in plants, including anthocyanin biosynthesis, vasculature system, cell signalling, seed maturation and abiotc stress responses. The present performed isolation and molecular cloning of full length Myb TF from Eleusine corocana. The isolated full-length coding sequence has 1053 bp and 350 aa was submitted to NCBI (Accession number MT312253). The transcript level of EcMYB increases under different abiotic stress treatments including dehydration, salinity, and high-temperature stress. The promoter region of EcMyb1 was found to be enriched in stress-responsive cis-regulatory elements such as DRE, HSE, ABRE etc. In phylogenetic analysis, EcMyb1 appeared to have high homology with its monocot orthologs particularly Sateria italica, Hordeum vulgare, Saccharum barberi and Oryza sativa. The three-dimension protein structure was generated based on homology modeling and structural aspects were discussed. Further, Insilco analysis was conducted to explore the physiological properties, subcellular localization, potential posttranslational modification sites (phosphorylation and glycosylation sites), and molecular and biological function of the full-length protein. Overall, the expression profiling and Insilco analysis of EcMyb1 strongly indicated its potential role in abiotic stress response in Eleusine corocana.

Published
2023

39. Recent Advances in Nanomaterials-Based Targeted Drug Delivery for Preclinical Cancer Diagnosis and Therapeutics.

Author

Tiwari, Harshita, Rai, Nilesh, Singh, Swati, Gupta, Priyamvada, Verma, Ashish, Singh, Akhilesh Kumar, Kajal, Salvi, Prafull, Singh, Santosh Kumar, and Gautam, Vibhav

Subjects
CANCER diagnosis, THERAPEUTICS, MEDICAL research, MULTIDRUG resistance, QUANTUM dots, TUMOR markers, LIPOSOMES, DENDRIMERS
Abstract

Nano-oncology is a branch of biomedical research and engineering that focuses on using nanotechnology in cancer diagnosis and treatment. Nanomaterials are extensively employed in the field of oncology because of their minute size and ultra-specificity. A wide range of nanocarriers, such as dendrimers, micelles, PEGylated liposomes, and polymeric nanoparticles are used to facilitate the efficient transport of anti-cancer drugs at the target tumor site. Real-time labeling and monitoring of cancer cells using quantum dots is essential for determining the level of therapy needed for treatment. The drug is targeted to the tumor site either by passive or active means. Passive targeting makes use of the tumor microenvironment and enhanced permeability and retention effect, while active targeting involves the use of ligand-coated nanoparticles. Nanotechnology is being used to diagnose the early stage of cancer by detecting cancer-specific biomarkers using tumor imaging. The implication of nanotechnology in cancer therapy employs photoinduced nanosensitizers, reverse multidrug resistance, and enabling efficient delivery of CRISPR/Cas9 and RNA molecules for therapeutic applications. However, despite recent advancements in nano-oncology, there is a need to delve deeper into the domain of designing and applying nanoparticles for improved cancer diagnostics. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

40. Intrinsically disordered protein, DNA binding with one finger transcription factor (OsDOF27) implicates thermotolerance in yeast and rice

Author

Gandass, Nishu, Kajal, and Salvi, Prafull

Subjects
Plant Science
Abstract

Intrinsically disorder regions or proteins (IDRs or IDPs) constitute a large subset of the eukaryotic proteome, which challenges the protein structure–function paradigm. These IDPs lack a stable tertiary structure, yet they play a crucial role in the diverse biological process of plants. This study represents the intrinsically disordered nature of a plant-specific DNA binding with one finger transcription factor (DOF-TF). Here, we have investigated the role of OsDOF27 and characterized it as an intrinsically disordered protein. Furthermore, the molecular role of OsDOF27 in thermal stress tolerance has been elucidated. The qRT-PCR analysis revealed that OsDOF27 was significantly upregulated under different abiotic stress treatments in rice, particularly under heat stress. The stress-responsive transcript induction of OsDOF27 was further correlated with enriched abiotic stress-related cis-regulatory elements present in its promoter region. The in vivo functional analysis of the potential role of OsDOF27 in thermotolerance was further studied in yeast and in planta. Ectopic expression of OsDOF27 in yeast implicates thermotolerance response. Furthermore, the rice transgenic lines with overexpressing OsDOF27 revealed a positive role in mitigating heat stress tolerance. Collectively, our results evidently show the intrinsically disorderedness in OsDOF27 and its role in thermal stress response in rice.

Published
2022
Full Text
View/download PDF

45. List of contributors

Author

Ade, Prasad, Aminedi, Raghavendra, Aung, Me Me, Aye, Myint, Badoni, Saurabh, Banik, Diptanu, Bartwal, Arti, Bharti, Mahesh Kumar, Bhat, Deepa, Bhati, Pradeep Kumar, Bhatt, Deepesh, Bhatt, Megha D., Bhattacharjee, Sougata, Bhowmick, Rakesh, Biswas, Koushik, Bohra, Abhishek, Butani, Naresh, Chakraborty, Anwesha, Chakrawarti, Neha, Chandra, Deepika, Chaudhary, Reeku, Chauhan, Harsh, Chung, Sang-Min, Dabral, Surbhi, Dagla, Manesh Chandra, Devi, Priyanka, Dey, Shipa Rani, Dhatwalia, Deepa, Dinesh, Govindaraj Kamalam, Dwivedi, Vikas, Elakkya, M., Elangovan, Allimuthu, Gantait, Saikat, Garg, Esha, Gusain, Suman, Harkhani, Khyati, Htun, Than Myint, Islam, Tofazzal, Ismail, Abdelbagi M., Jaiswal, V.P., Jha, Rajesh Kumar, Joshi, A.B., Joshi, Arun Kumar, Joshi, Rohit, Joshi, Shubham, Kalwan, Gopal, Kapadia, Chintan, Karthika, P., Kaur, Simardeep, Kesawat, Mahipal Singh, Khan, Laiba, Khan, M. Iqbal R., Koramutla, Murali Krishna, Kumar, Gaurav, Kumar, Gunti Vinod, Kumar, Manu, Kumar, Pankaj, Kumar, Pawan, Kumar, Prasann, Kumar, Sanjeev, Kumar, Suresh, Kumari, Anita, Kumari, Khushbu, Kumari, Priya, Mehta, Chandra Mohan, Mondal, Satyen, Mukherjee, Eashan, Nath, Jhilmil, Nath, Manoj, Nazir, Faroza, Negi, Manisha, Pal, Lalita, Palanisamy, Karvembu, Pandey, Indra Deo, Pandit, Jyotsana, Pandya, Nilesh, Patel, Helina, Patel, Nafisa, Patel, Rutu, Patra, Subhashree, Paul, Krishnayan, Quddus, Md. Ruhul, Ram, Chet, Ranjan, K., Rawat, Meenakshi, Rudakiya, Darshan M., Saikia, Purabi, Salvi, Prafull, Seem, Karishma, Selvan, Tamil, Shah, Gaurav, Shankar, Rama, Sharma, Anish Kumar, Sharma, Lalan, Shilky, Shukla, S.K., Siddique, R.A., Soni, Ravindra, Subrahmanyeswari, Tsama, Suprasanna, Penna, Tiwari, Abha, Trivedi, Sneha, Umar, Shahid, Vaishnav, Pujan B., Valarmathi, Ramanathan, Vasupalli, Naresh, Zar, Thu, and Zhu, Guanglong

Published
2024
Full Text
View/download PDF

47. Gateway cloning and in-planta transformation of drought stress responsive Ecmyb1 gene isolated from Eleusine coracana var.PRM 6107

Author

Bhatt, Megha, Salvi, Prafull, Lohani, Pushpa, Bhatt, Megha, Salvi, Prafull, and Lohani, Pushpa

Abstract

Drought is one of the key abiotic stress that critically influences the crops by restraining their growth and yield potential. Being sessile, plants tackle the detrimental effects of drought stress via modulating the cellular state by changing the gene expression. Such alteration of gene expression is essentially driven by the transcriptional syndicate. Transcription factors (TF) are the key regulatory protein that controls the expression of their target gene by binding to the cis-regulatory elements present in the promoter region. Myb-TFs ubiquitously present in all eukaryotes belong to one of the largest TF family, and play wide array of biological functions in plants including anthocyanin biosynthesis, vasculature system, cell signaling, seed maturation and abiotc stress responses. In the present study the full length Myb TF from Eleusine corocana was subcloned using Gateway cloning system and further transformed into Arabidopsis thaliana through floral dip method. Transgenic Arabidopsis thaliana plants harbouring Ecmyb1 gene were screened and grown in transgenic glasshouse under controlled conditions.

Published
2021

49. Advancement in the molecular perspective of plant-endophytic interaction to mitigate drought stress in plants.

Author

Salvi, Prafull, Mahawar, Himanshu, Agarrwal, Ruchi, Kajal, Gautam, Vibhav, and Deshmukh, Rupesh

Subjects
DROUGHTS, DROUGHT management, DROUGHT tolerance, FUNCTIONAL genomics, WHOLE genome sequencing, WATER shortages, COMPARATIVE genomics
Abstract

Change in global climate has started to show its effect in the form of extremes of temperatures and water scarcity which is bound to impact adversely the global food security in near future. In the current review we discuss the impact of drought on plants and highlight the ability of endophytes, microbes that inhabit the plants asymptomatically, to confer stress tolerance to their host. For this we first describe the symbiotic association between plant and the endophytes and then focus on the molecular and physiological strategies/mechanisms adopted by these endophytes to confer stress tolerance. These include root alteration, osmotic adjustment, ROS scavenging, detoxification, production of phytohormones, and promoting plant growth under adverse conditions. The review further elaborates on how omics-based techniques have advanced our understanding of molecular basis of endophyte mediated drought tolerance of host plant. Detailed analysis of whole genome sequences of endophytes followed by comparative genomics facilitates in identification of genes involved in endophyte-host interaction while functional genomics further unveils the microbial targets that can be exploited for enhancing the stress tolerance of the host. Thus, an amalgamation of endophytes with other sustainable agricultural practices seems to be an appeasing approach to produce climate-resilient crops. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results