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21 results on '"Sambe-Ba, Bissoume"'

1. High Prevalence of Virulence Genes and <i>in Vitro</i> Biofilm Production in Clinical Multidrug-Resistant <i>Escherichia coli</i> in Dakar Senegal

Author

Mawunyo Dossouvi, Komla, primary, Sambe Ba, Bissoume, additional, Lo, Gora, additional, Ndiaye, Issa, additional, Ba-Diallo, Awa, additional, Sow, Ousmane, additional, Dieng, Assane, additional, Talla, Fatimata, additional, Cissé, Abdoulaye, additional, Mbaye Lo Ndiaye, Serigne, additional, Tine, Alioune, additional, Karam, Farba, additional, Diagne-Samb, Habsa, additional, Ngom-Cisse, Safietou, additional, Diop-Ndiaye, Halimatou, additional, Maman, Issaka, additional, Toure-Kane, Coumba, additional, Gaye-Diallo, Aïssatou, additional, Dossim, Sika, additional, Mboup, Souleymane, additional, Saad Bouh Boye, Cheikh, additional, Seck, Abdoulaye, additional, and Camara, Makhtar, additional

Published
2023
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2. African women in science and development, bridging the gender gap

Author

Founou, Luria Leslie, primary, Yamba, Kaunda, additional, Kouamou, Vinie, additional, Asare Yeboah, Esther Eyram, additional, Saidy, Binta, additional, Jawara, Lalia A., additional, Bah, Haddy, additional, Sambe Ba, Bissoume, additional, Aworh, Mabel Kamweli, additional, and Darboe, Saffiatou, additional

Published
2023
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13. First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal

Author

Diop, Amadou, primary, Sambe-Ba, Bissoume, additional, Seck, Abdoulaye, additional, Dia, Mouhamadou Lamine, additional, Timbiné, Lassina Gadi, additional, Niang, Aïssatou Ameth, additional, Ndiaye, El Hadji Momar, additional, Sonko, Mouhamadou Abdoulaye, additional, Wane, Abdoul Aziz, additional, Bercion, Raymond, additional, Ndiaye, Ousmane, additional, Cissé, Moussa Fafa, additional, and Gassama-Sow, Amy, additional

Published
2016
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14. High-quality draft genome sequence and description of Haemophilus massiliensis sp. nov.

Author

Lo, Cheikh Ibrahima, primary, Sankar, Senthil Alias, additional, Fall, Bécaye, additional, Sambe-Ba, Bissoume, additional, Diawara, Silman, additional, Gueye, Mamadou Wague, additional, Mediannikov, Oleg, additional, Blanc-Tailleur, Caroline, additional, Wade, Boubacar, additional, Raoult, Didier, additional, Fournier, Pierre-Edouard, additional, and Fenollar, Florence, additional

Published
2016
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15. MALDI-TOF Mass Spectrometry: A Powerful Tool for Clinical Microbiology at Hôpital Principal de Dakar, Senegal (West Africa)

Author

Lo, Cheikh I., primary, Fall, Bécaye, additional, Sambe-Ba, Bissoume, additional, Diawara, Silman, additional, Gueye, Mamadou W., additional, Mediannikov, Oleg, additional, Sokhna, Cheikh, additional, Faye, Ngor, additional, Diemé, Yaya, additional, Wade, Boubacar, additional, Raoult, Didier, additional, and Fenollar, Florence, additional

Published
2015
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16. Antimicrobial Resistance Profile of SalmonellaIsolates in Chicken Carcasses in Dakar, Senegal

Author

Fall-Niang, Ndeye Khota, Sambe-Ba, Bissoume, Seck, Abdoulaye, Deme, Saidou Nourou, Wane, Abdoul Aziz, Bercion, Raymond, Alambedji-Bada, Rianatou, and Gassama-Sow, Amy

Abstract

AbstractThe incidence of the Salmonellacontamination of poultry products in Senegal is unknown. Salmonellacontamination and antimicrobial drug resistance profiles in chicken carcasses were investigated. Between July 2012 and July 2013, three types of chicken carcasses (broilers, laying hens, and premises chickens) obtained from retailers in the markets of Dakar and its suburbs were tested for Salmonellacontamination. Salmonellastrains were isolated from 300 chicken carcasses according to International Organization for Standardization ISO 6579 (2002) guidelines. In these samples, 273 isolates were obtained, belonging to 22 serovars, and 53% samples were contaminated with at least 1 serovar. Standardized techniques were used for the susceptibility testing and serotyping of isolates. Hygiene conditions, in terms of the cleanliness of stalls, the packing of chicken carcasses in bags, and the maintenance of the cold chain at the stall, were moderately poor. The three serovars most frequently identified were SalmonellaIstanbul (28%), SalmonellaBrancaster (19%), and SalmonellaKentucky (13%). Overall, 21% of isolates were resistant to quinolones and fluoroquinolones. Serovar Istanbul was resistant to tetracycline (TE) and trimethoprim + sulfamethoxazole (SXT). Serovars Brancaster and Kentucky were resistant to betalactams and to quinolones or fluoroquinolones. The uncommon serovar Senftenberg had the strongest resistance profile, displaying resistance to betalactams including imipenem (IMP). Large numbers of isolates were resistant to TE (66%) and SXT (47%). Resistance to cephalosporins (5%), chloramphenicol (2%), gentamicin (8%), and IMP (1%) was less frequent. A large proportion of the broilers sold in Dakar markets were contaminated with Salmonella. This situation probably resulted from poor hygiene conditions in chicken farms and slaughterhouses and from breaks in the cold chain at some point in the distribution of poultry products.

Published
2019
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17. Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

Author

Sambou, Masse, primary, Aubadie-Ladrix, Maxence, additional, Fenollar, Florence, additional, Fall, Becaye, additional, Bassene, Hubert, additional, Almeras, Lionel, additional, Sambe-Ba, Bissoume, additional, Perrot, Nadine, additional, Chatellier, Sonia, additional, Faye, Ngor, additional, Parola, Philippe, additional, Wade, Boubacar, additional, Raoult, Didier, additional, and Mediannikov, Oleg, additional

Published
2015
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18. Identification of Atypical El Tor V. cholerae O1 Ogawa Hosting SXT Element in Senegal, Africa.

Author

Sambe-Ba, Bissoume, Diallo, Mamadou H., Seck, Abdoulaye, Wane, Abdoul A., de Magny, Guillaume Constantin, Boye, Cheikh S.-B., Sow, Ahmad I., and Gassama-Sow, Amy

Subjects
VIBRIO cholerae, CHOLERA, PHENOTYPES, VIBRIO, VIBRIO infections
Abstract

Vibrio cholerae O1 is the causative agent of cholera with classical and El Tor, two well-established biotypes. In last 20 years, hybrid strains of classical and El Tor and variant El Tor which carry classical ctxB have emerged worldwide. In 2004-2005, Senegal experienced major cholera epidemic with a number of cases totalling more than 31719 with approximately 458 fatal outcomes (CFR, 1.44%). In this retrospective study, fifty isolates out of a total of 403 V. cholerae biotype El Tor serovar Ogawa isolates from all areas in Senegal during the 2004-2005 cholera outbreak were randomly selected. Isolates were characterized using phenotypic and genotypic methods. The analysis of antibiotic resistance patterns revealed the predominance of the S-Su-TCY-Tsu phenotype (90% of isolates). The molecular characterization of antibiotic resistance revealed the presence of the SXT element, a self-transmissible chromosomally integrating element in all isolates. Most of V. cholerae isolates had an intact virulence cassette (86%) (ctx, zot, ace genes). All isolates tested gave amplification with primers for classical CT, and 10/50 (20%) of isolates carried classical and El Tor ctxB. The study reveals the presence of atypical V. cholerae O1 El Tor during cholera outbreak in Senegal in 2004-2005. [ABSTRACT FROM AUTHOR]

Published
2017
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20. First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal.

Author

Diop, Amadou, Sambe-Ba, Bissoume, Seck, Abdoulaye, Dia, Mouhamadou Lamine, Timbiné, Lassina Gadi, Niang, Aïssatou Ameth, Ndiaye, El Hadji Momar, Sonko, Mouhamadou Abdoulaye, Wane, Abdoul Aziz, Bercion, Raymond, Ndiaye, Ousmane, Cissé, Moussa Fafa, and Gassama-Sow, Amy

Subjects
*SALMONELLA, *BETA lactamases, *NOSOCOMIAL infections, *HOSPITAL patients, *ESCHERICHIA coli
Abstract

Nosocomial infections are very common in African hospitals, particularly in neonatal units. These infections are most often caused by bacteria such as Escherichia coli, Klebsiella spp and Staphylococcus spp. Salmonella strains are rarely involved in nosocomial infections. Here, we report the first description of S. Grumpensis in neonatal infections in Senegal. Seventeen Salmonella strains were isolated from hospitalized infants’ stool samples. The following resistance phenotype was described in strains: AMXRTICRCFR FOXRCFXRCTXRCAZRIMPSATMRNARNORRCIPRTMRGMRTERSXTR. All isolates were susceptible to imipenem, 15 out of 17 produced an extended spectrum ß-lactamase (ESBL). blaOXA-1, blaSHV-1, blaTEM-1, blaCTX-M1 genes were detected in strains 8, 13, 5 and 8, respectively. blaCTX-M1 sequencing revealed the presence of blaCTX-M-109. Thirteen of the 17 Salmonella Grumpensis strains were analyzed by PFGE. These 13 isolates belonged to a single pulsotype and were genotypically identical. This is the first report of neonatal S. Grumpensis infections in Senegal, and the first report of blaCTX-M-109 in the genus Salmonella. [ABSTRACT FROM AUTHOR]

Published
2016
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21. Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides(Diptera: Ceratopogonidae) Biting Midges in Senegal

Author

Sambou, Masse, Aubadie-Ladrix, Maxence, Fenollar, Florence, Fall, Becaye, Bassene, Hubert, Almeras, Lionel, Sambe-Ba, Bissoume, Perrot, Nadine, Chatellier, Sonia, Faye, Ngor, Parola, Philippe, Wade, Boubacar, Raoult, Didier, and Mediannikov, Oleg

Abstract

ABSTRACTBiting midges of the genus Culicoidesare implicated as vectors for a wide variety of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in Africa. However, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthropod identification at the species level. This study established a spectrum database of Culicoidesspp. from Senegal using MALDI-TOF. Identification of Culicoidesinsects to the species level before mass spectrometry was performed on the basis of morphological characters. MALDI-TOF MS reference spectra were determined for 437 field-caught Culicoidesof 10 species. The protein profiles of all tested Culicoidesrevealed several peaks with mass ranges of 2 to 20 kDa. In a validation study, 72 Culicoidesspecimens in the target species were correctly identified at the species level with a similarity of 95 to 99.9%. Four Culicoidesprotein profiles were misidentified. Nevertheless, six SuperSpectra (C. imicola, C. enderleini, C. oxystoma, C. kingi, C. magnus, and C. fulvithorax) were created. Abdomens of midges were used to amplify and sequence a portion of the mitochondrial cytochrome oxidase I gene (COI). The results obtained using the MALDI-TOF MS method were consistent with the morphological identification and similar to the genetic identification. Protein profiling using MALDI-TOF is an efficient approach for the identification of Culicoidesspp., and it is economically advantageous for approaches that require detailed and quantitative information of vector species that are collected in field. The database of African CulicoidesMS spectra created is the first database in Africa. The COI sequences of five Culicoidesspecies that were previously noncharacterized using molecular methods were deposited in GenBank.

Published
2015
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