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Background Plasmodium falciparum malaria is associated with anaemia-related morbidity, attributable to host, parasite and drug factors. We quantified the haematological response following treatment of uncomplicated P. falciparum malaria to identify the factors associated with malarial anaemia. Methods Individual patient data from eligible antimalarial efficacy studies of uncomplicated P. falciparum malaria, available through the WorldWide Antimalarial Resistance Network data repository prior to August 2015, were pooled using standardised methodology. The haematological response over time was quantified using a multivariable linear mixed effects model with nonlinear terms for time, and the model was then used to estimate the mean haemoglobin at day of nadir and day 7. Multivariable logistic regression quantified risk factors for moderately severe anaemia (haemoglobin < 7 g/dL) at day 0, day 3 and day 7 as well as a fractional fall ≥ 25% at day 3 and day 7. Results A total of 70,226 patients, recruited into 200 studies between 1991 and 2013, were included in the analysis: 50,859 (72.4%) enrolled in Africa, 18,451 (26.3%) in Asia and 916 (1.3%) in South America. The median haemoglobin concentration at presentation was 9.9 g/dL (range 5.0–19.7 g/dL) in Africa, 11.6 g/dL (range 5.0–20.0 g/dL) in Asia and 12.3 g/dL (range 6.9–17.9 g/dL) in South America. Moderately severe anaemia (Hb < 7g/dl) was present in 8.4% (4284/50,859) of patients from Africa, 3.3% (606/18,451) from Asia and 0.1% (1/916) from South America. The nadir haemoglobin occurred on day 2 post treatment with a mean fall from baseline of 0.57 g/dL in Africa and 1.13 g/dL in Asia. Independent risk factors for moderately severe anaemia on day 7, in both Africa and Asia, included moderately severe anaemia at baseline (adjusted odds ratio (AOR) = 16.10 and AOR = 23.00, respectively), young age (age < 1 compared to ≥ 12 years AOR = 12.81 and AOR = 6.79, respectively), high parasitaemia (AOR = 1.78 and AOR = 1.58, respectively) and delayed parasite clearance (AOR = 2.44 and AOR = 2.59, respectively). In Asia, patients treated with an artemisinin-based regimen were at significantly greater risk of moderately severe anaemia on day 7 compared to those treated with a non-artemisinin-based regimen (AOR = 2.06 [95%CI 1.39–3.05], p < 0.001). Conclusions In patients with uncomplicated P. falciparum malaria, the nadir haemoglobin occurs 2 days after starting treatment. Although artemisinin-based treatments increase the rate of parasite clearance, in Asia they are associated with a greater risk of anaemia during recovery.

BackgroundIntestinal parasitic infections remain of public health concern worldwide, especially among rural and poorest populations as a consequence of precariousness, lack of sanitation, non-availability of potable water and poor hygiene conditions. The present study aimed to better understand the epidemiology of intestinal parasitic infections in rural areas of forested Cameroon.MethodologyA cross-sectional survey was conducted in three Health Areas (Ndelele, Kentzou and Lolo) of the Ndelele Health District (East Region, Cameroon). Information on socio-demographic characteristics, knowledge, attitudes, and practices regarding intestinal parasitic infections were collected using a semi-structured questionnaire. Stool samples were collected and analyzed by the Kato-Katz and formalin-ether concentration techniques to complement simple direct examination.Principal FindingsA total of 406 individuals belonging to three main groups (Kako or Bantu, Baka or Pygmies and Central African Republic refugees) were enrolled in the study. The overall intestinal parasitic infection rate was 74.9%, including 57.2% cases of polyparasitism. Fourteen parasite species were identified, 89.1% being intestinal protozoa and 41.8% belonging to helminths. Infections with helminths were associated with Baka (P < 0.0001). Spring water consumption was associated with hookworm infection (OR = 3.87; P = 0.008). Garbage deposited near houses was positively associated with infection with Giardia lamblia (OR = 3.41; P = 0.003). Polyparasitism was positively linked to washing hand without soap before meal (OR= 11.64; p= 0.002).Conclusion/SignificanceIntestinal parasitic infections exhibited high rates in the Ndelele Health District, especially among indigenous and hard-to-reach populations (Pygmies). Hygiene measures appear as the main drivers sustaining transmission, and targeted strategies should be developed to efficiently fight against these debilitating diseases.Author summaryIntestinal parasitic infections such as soil transmitted helminthiasis and schistosomiasis, remain a public health concern in Cameroon. To better understand the epidemiology of these infections in hard-to-reach populations in rural areas, a cross-sectional study was carried out in three health areas of Ndelele health District in the Eastern-Cameroon. The rate of intestinal parasitic infections was high (74.9%) especially in indigenous populations (Baka-Pygmies) which exhibited high prevalence of soil transmitted helminth infections (83.3%). The study confirmed that the lack of sanitation and poor hygiene largely contribute to the endemicity of intestinal parasitic infections, particularly among indigenous populations. Targeted control strategies seem mandatory to reach these populations and offer them appropriate care to interrupt or reduce the transmission of these diseases.

Background Female Genital Schistosomiasis (FGS) is most often caused by presence of Schistosoma haematobium eggs lodged in the female reproductive tract which results in chronic fibrosis and scarring. In Cameroon, despite high community prevalences of urine-patent S. haematobium infections, FGS has yet to be studied in depth. To shed light on the clinical prevalence and socioeconomic effects of FGS, we undertook a formative community-based epidemiological and qualitative survey. Method A cross sectional multidisciplinary study of 304 girls and women from 11 remote rural fishing communities in Cameroon was undertaken using parasitological sampling, clinical colposcopy, and interviews. The lived experiences of those with FGS were documented using a process of ethnography with participant observation and in-depth interviews. Result Amongst 304 women and girls aged >5 years (Median age: 18; Interquartile range: 9.6–28), 198 females were eligible for FGS testing and 58 adult women were examined by clinical colposcopy. Of these, 34 were positive for FGS (proportion: 58.6%; 95% CI: 45.8–70.4), younger girls showing a higher FGS prevalence, and older women not shedding eggs showing a pattern for cervical lesions from earlier infection. In a subset of women with FGS selected purposively (12/58), in-depth interviews with participant observation revealed out-of-pocket expenditures of up to 500USD related health spending for repeated diagnosis and treatment of gynecological illnesses, and 9 hours daily lost reproductive labour. Psychosocial unrest, loss in social capital, and despair were linked with sub-fertility and persistent vaginal itch. Conclusion With our first formative evidence on prevalence, socioeconomic effects and experiences of FGS amongst women and girls in Cameroon, we have clarified to a new level of detail the deficit in provision of and access to peripheral health services in remote areas of Cameroon. Using this information, there is now strong evidence for national programs and services on women’s health and schistosomiasis to update and revise policies targeted on prevention and management of FGS. We therefore stress the need for regular provision of Praziquantel treatment to adolescent girls and women in S. haematobium endemic areas, alongside better access to tailored diagnostic services that can detect FGS and appropriately triage care at primary health level.

Intestinal parasitic infections (IPIs) remain a public health issue in developing countries where overcrowded settlements and poor sanitation are general rule. Due to paucity of IPIs data in known overcrowded Cameroonian prisons, this cross-sectional study conducted in 2015 in the New-Bell Central Prison (NBCP) aimed to establish biodiversity, prevalence and risk factors of intestinal protozoan and helminthe infections among inmates. Fresh stool samples collected from the NBCP volunteered inmates were laboratory examined microscopically as fresh mounts plus iodine, Kato-Katz smears, formalin-ether concentration and modified Ziehl-Nelseen stained sediments. Of a total 374 inmates who participated in the study, overall IPIs prevalence was 39.3%. Helminthe and protozoa prevalence was 16.6% and 24.6% respectively. Parasites species were recorded at following prevalence: Ascaris lumbricoides (10.4%), Trichuris trichiura (5.1%), Schistosoma mansoni (0.5%), Entamoeba histolytica/dispar (14.2%), Entamoeba coli (16.6%), Giardia intestinalis (7.2%), Chilomastix mesnili (2.4%), Blastocystis spp (2.1%) and Cryptosporidium sp (4.3%). Co-infections by two or three parasites were recorded among parasitized inmates. Overall IPIs prevalence was not significantly influenced by gender, age, detention duration, education level, handwashing practices, sanitation and drinking water source. However, highest IPIs prevalence occurred in males aged 30 to 49 years old, less than one year detainees, latrine users and those who drank borehole water. Systematic handwashing practices and education level did not influence significantly IPIs prevalence. All helminthe infections were of light intensities. Inmates in the New Bell central prison were parasitized by several species of protozoa and intestinal worms in varying prevalence depending on the detention period, the sex, the age and hygiene. A regular IPIs control among prison inmates was recommended to the NBCP managers to prevent related morbidity.

During pregnancy, the hematological system undergoes numerous changes so as to meet up with the demands of the developing fetus and placenta, with major alterations in blood volume and this differs with women from different regions. The aim of this study was therefore to assess the hematological parameters and risk factors for anemia among pregnant women according to different trimesters of pregnancy in Douala, Cameroon. Methods: A cross-sectional study was conducted from February to May 2017, and all pregnant women who attended antenatal visits during our study period and who suited our inclusion criteria were recruited. The study was carried out in the antenatal care Unit of the Douala Laquintinie Hospital (DLH). A pretested questionnaire was used for the necessary data collection. Venous blood was collected from each of these women to perform a Complete Blood Count (CBC) test using an automated hematological analyzer (URIT 3010). Data were analyzed using XLSTAT 2007 and Stata version 11 software. Results: The mean age of the participants was 28 (SD = 5 years). The prevalence of anemia among pregnant women was 22% with a majority (18.4%) of these women being mildly anemic. Mean Hemoglobin values were significantly higher among women in first trimester compared to the third (12.1 ± 0.9 g/dl vs 11.8 ± 1.3 g/dl; p = 0.043). There was also a significant change in mean hematocrit (HCT) values between the first and second trimester (32.8% ± 2.5% vs 31.4% ± 2.9%, p = 0.004) and between the first and third trimester (32.8% ± 2.5% vs 30.8% ± 3.5%, p -4). RBC count value was higher in the first trimester than in the second trimester (3.7 ± 0.3 × 1012/L vs 3.5 ± 0.4 × 1012/L, p -4) and in the third trimester (3.7 ± 0.3 × 1012/L vs 3.5 ± 0.4 × 1012/L, p = 0.001). After a multivariate analysis, the following categories of women had more odds of developing anemia; women between the age range of 30 - 35 (OR = 2.81, 95%CI: 1.16 - 6.81, p = 0.023), women in the second trimester of pregnancy (OR = 2.20, 95%CI: 0.88 - 5.48, p = 0.024), women with blood group O (OR = 3.57, 95%CI: 1.41 - 16.66, p = 0.012). Conclusion: This study confirms significant variations in hematological parameters. The findings reinforce the need for supplementation and provide additional information on hematological reference values in pregnancy in Cameroon. It also helps us understand that, third trimester, age range 30 - 35, and blood group may be potential risk factors associated with anemia in pregnancy though a cohort study would be necessary to ascertain this hypothesis.

Introduction. Malaria is and remains a serious health concern in Africa. In Cameroon, where malaria is endemic and a major public health problem, the major control measure put in place is the use of long-lasting insecticidal nets (LLINs). In the Tiko Health District (THD), the challenges have been to assess and to evaluate the ownership and utilisation of LLINs. This study sought to assess the ownership and utilisation rates of LLINs in the THD. Methodology. A cross-sectional survey involving 418 households was conducted in four health areas in the THD. A structured questionnaire was used to collect data on LLIN ownership and utilisation as well as sociodemographic characteristics. Results. The ownership of at least one LLIN per household, coverage, and accessibility were, respectively, 89%, 56.2%, and 66.3%, while installing LLINs on all beds in the household, sleeping under LLINs the previous night (SULPN), and universal utilisation were 72%, 24.9%, and 14.1%, respectively. Factors significantly associated with the ownership of at least one LLIN per household were respondent’s age and gender. Heat (21.1%) and forgetfulness (6.5%) were the main reasons postulated for irregular utilisation of LLINs. Conclusion. The ownership LLINs failed to guarantee utilisation and definitely effective control of malaria in the THD, as expected. Continuous and appropriate use of LLINs is indispensable, in addition to periodic sanitation, booster campaigns of LLIN distribution, and evaluation research for effective prevention and control of malaria.

Background Incomplete adherence to antiretroviral therapy (ART) is one of the factors that contribute to HIV drug resistance, and it is a major problem for the public health system in controlling the HIV pandemic. There is emerging evidence that SMS can play an important role in health care delivery among patients with HIV on ART, especially in resource-limited settings. Objective This paper aims to assess the impact of two-way and one-way SMS text messaging on adherence to HIV treatment. We hypothesized that sending weekly text messages through the one-way and two-way SMS text messaging approach will improve adherence to ART among patients with HIV and improve associated clinical outcomes (quality of life). Methods A randomized controlled trial is being carried out among participants with HIV who have been on ART for at least one month from an accredited treatment center, namely the Buea Regional Hospital and Kumba District Hospital of South West Region, Cameroon. Participants with HIV, both male and female, aged 21 years and older make up a sample size of 207. The interventions involved the use of mobile phone text messages. Before commencing the intervention, a focus group discussion was carried out among the participants to understand their perception about the use of SMS-based interventions to improve adherence. A total of 246 participants were randomized to receive either a one-way text message (SMS sent to a recipient without recipient sending a reply) or two-way text message (SMS sent to a recipient and recipient sends a reply) or the control (no SMS, only standard care). Data on adherence and quality of life were collected at baseline and after 6 months and will be analyzed using SPSS version 21, while qualitative data will be analyzed using Atlas.ti 7.5. Results Data collection began in September 2019 with focus group discussions and baseline data collection. After 1 month of baseline data collection, the intervention began in October 2019, and postintervention data were collected after 6 months (March 2020). At the end of the study, we will be able to understand the perception of patients toward SMS text messaging–based interventions and also assess the impact of one-way and two-way SMS text messages on treatment adherence among patients with HIV and on associated clinical outcomes (quality of life). Conclusions The impact of SMS text messaging varies across different settings. The results from this study will determine the perception of patients toward an SMS text messaging–based intervention and its impact on adherence to ART. International Registered Report Identifier (IRRID) DERR1-10.2196/16127

Malaria still has a devastating impact on public health and welfare in Cameroon. Despite the increasing number of studies conducted on disease prevalence, transmission patterns or treatment, there are to date, not enough studies summarising findings from previous works in order to identify gaps in knowledge and areas of interest where further evidence is needed to drive malaria elimination efforts. The present study seeks to address these gaps by providing a review of studies conducted so far on malaria in Cameroon since the 1940s to date. Over 250 scientific publications were consulted for this purpose. Although there has been increased scale-up of vector control interventions which significantly reduced the morbidity and mortality to malaria across the country from a prevalence of 41% of the population reporting at least one malaria case episode in 2000 to a prevalence of 24% in 2017, the situation is not yet under control. There is a high variability in disease endemicity between epidemiological settings with prevalence of Plasmodium parasitaemia varying from 7 to 85% in children aged 6 months to 15 years after long-lasting insecticidal nets (LLINs) scale-up. Four species of Plasmodium have been recorded across the country: Plasmodium falciparum, P. malariae, P. ovale and P. vivax. Several primate-infecting Plasmodium spp. are also circulating in Cameroon. A decline of artemisinin-based combinations therapeutic efficacy from 97% in 2006 to 90% in 2016 have been reported. Several mutations in the P. falciparum chloroquine resistance (Pfcrt) and P. falciparum multidrug resistance 1 (Pfmdr1) genes conferring resistance to either 4-amino-quinoleine, mefloquine, halofanthrine and quinine have been documented. Mutations in the Pfdhfr and Pfdhps genes involved in sulfadoxine-pyrimethamine are also on the rise. No mutation associated with artemisinin resistance has been recorded. Sixteen anopheline species contribute to malaria parasite transmission with six recognized as major vectors: An. gambiae, An. coluzzii, An. arabiensis, An. funestus, An. nili and An. moucheti. Studies conducted so far, indicated rapid expansion of DDT, pyrethroid and carbamate resistance in An. gambiae, An. coluzzii, An. arabiensis and An. funestus threatening the performance of LLINs. This review highlights the complex situation of malaria in Cameroon and the need to urgently implement and reinforce integrated control strategies in different epidemiological settings, as part of the substantial efforts to consolidate gains and advance towards malaria elimination in the country.

The formalization of Cameroon's health policy has been largely prescribed by international institutions for several decades. Emblematic of the era of governance and partnership in countries with limited resources (Guilbaud, 2015), it places the public sector in a paradoxical situation, where the State called upon to stimulate, lead and supervise public action must develop balanced relations with its partners. Between this partnership dynamic that governs the State (Eboko, 2015) and the proces...

La formalisation de la politique de santé du Cameroun est en grande partie prescrite par les institutions internationales depuis plusieurs décennies. Emblématique de l’ère de la gouvernance et du partenariat dans les pays à ressources limitées (Guilbaud, 2015), elle place le secteur public dans une situation paradoxale, où l’Etat appelé à impulser, animer, encadrer l’action publique, doit aménager des relations équilibrées avec ses partenaires. Entre cette dynamique partenariale qui encadre l...

Introduction: Blastocystis spp. is a protist found in humans. Although usually the most frequent protozoa found in stool samples of both symptomatic and healthy subjects, its pathogenic or rather opportunistic role is yet to be clearly elucidated. To attempt to fill this gap, a cross-sectional study was conducted to compare the frequency of Blastocystis spp. in HIV positive (HIV+) versus HIV negative (HIV-) individuals in four health facilities of the Center Region of Cameroon. Methodology: Stool samples were collected from 283 HIV positive and 245 HIV negative subjects and analyzed using direct diagnostic tests. Results: A total of 46 (8.7%) individuals were found infected with Blastocystis spp., including 6.7% HIV positive and 11.0% HIV negative. This species was more frequent in urban and semi-urban areas than in rural areas, but evenly distributed among genders and age groups as well as among all sectors of activity. The prevalence of Blastocystis spp. (11.3%) was higher in HIV+ patients with a CD4 count ≥ 500 cells / mm3, but no significant difference was found among HIV clinical stages. Likewise prevalence, the mean number of cysts per gram of stool was similar between HIV positive and HIV negative individuals. People infected with Blastocystis spp. showed diverse clinical signs, but only flatulence was significantly more prevalent. The frequencies of these clinical signs were not related to HIV status. Conclusion: No clear relationship links the infection with Blastocystis spp. to HIV, although its presence was associated with digestive disorder, suggesting that this parasite might not be opportunist.

BACKGROUND Incomplete adherence to antiretroviral therapy (ART) is one of the factors that contribute to HIV drug resistance, and it is a major problem for the public health system in controlling the HIV pandemic. There is emerging evidence that SMS can play an important role in health care delivery among patients with HIV on ART, especially in resource-limited settings. OBJECTIVE This paper aims to assess the impact of two-way and one-way SMS text messaging on adherence to HIV treatment. We hypothesized that sending weekly text messages through the one-way and two-way SMS text messaging approach will improve adherence to ART among patients with HIV and improve associated clinical outcomes (quality of life). METHODS A randomized controlled trial is being carried out among participants with HIV who have been on ART for at least one month from an accredited treatment center, namely the Buea Regional Hospital and Kumba District Hospital of South West Region, Cameroon. Participants with HIV, both male and female, aged 21 years and older make up a sample size of 207. The interventions involved the use of mobile phone text messages. Before commencing the intervention, a focus group discussion was carried out among the participants to understand their perception about the use of SMS-based interventions to improve adherence. A total of 246 participants were randomized to receive either a one-way text message (SMS sent to a recipient without recipient sending a reply) or two-way text message (SMS sent to a recipient and recipient sends a reply) or the control (no SMS, only standard care). Data on adherence and quality of life were collected at baseline and after 6 months and will be analyzed using SPSS version 21, while qualitative data will be analyzed using Atlas.ti 7.5. RESULTS Data collection began in September 2019 with focus group discussions and baseline data collection. After 1 month of baseline data collection, the intervention began in October 2019, and postintervention data were collected after 6 months (March 2020). At the end of the study, we will be able to understand the perception of patients toward SMS text messaging–based interventions and also assess the impact of one-way and two-way SMS text messages on treatment adherence among patients with HIV and on associated clinical outcomes (quality of life). CONCLUSIONS The impact of SMS text messaging varies across different settings. The results from this study will determine the perception of patients toward an SMS text messaging–based intervention and its impact on adherence to ART. INTERNATIONAL REGISTERED REPORT DERR1-10.2196/16127

Background: Nosocomial infection’s (NIs) data are scarce in developing countries. In some of these countries, national guidelines for its prevention by health facilities have introduced surveillance recommendations including Infection Control Assessment Tool (ICAT). The aim of our study was to evaluate the compliance levels of NIs recommendations. Methodology: We conducted a cross-sectional study from September to December 2018 in 10 health facilities in Yaounde. A checklist with two modules from the ICAT (Health Facility information and Infection Control Program) was used to determine the degree of compliance towards the recommendations by performing interviews, observations and consultation of documents. Results: Sixty (60) % of health facilities are aware of the national guidelines and regulation on the fight against NIs but only 30% have adopted them. Accreditation standards applicable to health facilities are not known by any of the health facilities. The recommendations concerning demographic characteristics, water supply and the general characteristics on rooms were generally respected (more than 50%) in 90% of the health facilities. 100% of health facilities had less than fifty (50)% compliance level for the fight against NIs recommendations with compliance levels below 15% for 50% of them. This worst compliance (less than 15%) was especially observed with recommended practices concerning responsibilities and authorities (40% of health facilities), functionality of infection and control committees (50% of health facilities), key personnel responsible for fight against NIs (30% of health facilities) and study of outbreaks and surveillance of NIs (100% of health facilities). Training programs on fight against NIs had better scores (30% of health facilities with more than 50% of compliance levels). The Fischer test shows that there is a significant relationship between the compliance with all these recommended practices and the health facility capacity (P= 0, 0476) . Conclusion: NIs control programs in Yaounde health facilities are insufficient. Awareness, training, promotion and follow-up actions are necessary for the understanding and adoption of recommendations on the monitoring of NIs.

Background: Depression of the immune system caused by the human immunodeficiency virus (HIV) promotes the onset of opportunistic infections including intestinal parasites. Objectives: In this study, we are comparing the prevalence of these parasitic infections among individuals infected with HIV (HIV+) and non-infected (HIV-) enrolled in four HIV care units in the Centre Region of Cameroon. Methods: A cross-sectional study was conducted and stool samples were collected from 283 HIV positive subjects and 245 seronegative subjects. These samples were processed using direct wet mount, formol-ether concentration, Kato-Katz procedure, modified Ziehl-Neelsen staining, and Baermann techniques to identify both common and opportunistic intestinal parasites. Logistic regression analyses were used to assess the association between HIV infection and socio-demographics factors as well as infection with intestinal parasites. Results: A total of 123 (23.4%) individuals were found infected with at least one protozoan parasite species, and nine (1.7%) with intestinal worms. Overall, infection rates were 27.9% and 22.4% among HIV+ and HIV- subjects, respectively. The frequencies of Cryptosporidium spp. and Entamoeba histolytica/dispar were significantly more important among HIV+ subjects (P < 0.0455). Pentatrichomonas hominis and Entamoeba coli were significantly more prevalent among HIV- subjects (P < 0.0210). Heterogeneity was observed in the distribution of intestinal parasites, according to socio-demographic parameters. In addition, Cryptosporidium spp. was significantly associated with the decrease in CD4 cell count (P = 0.0035). The frequencies of infections with Cryptosporidium spp., Isospora belli, and Iodamoeba buetschlii were significantly more important in HIV positive patients not taking antiretroviral (P < 0.0226). Conclusions: Cryptosporidium spp., Entamoeba histolytica/dispar, and Blastocystis spp. were the intestinal pathogens more often found among HIV infected individuals. HIV positive subjects were significantly more infected with intestinal parasites than seronegatives in intermediate and rural settings, among individuals aged more than 45 years and among unemployed individuals.

Schistosomiasis is endemic in Cameroon with three Schistosoma species infecting human in many areas. Data from many countries endemic for S.mansoni, S.haematobium and S.japonicum reported an age-dependent antibody response profile using homologous adult schistosome antigen extract. Less is known about the antibody profile in schistosomiasis endemic areas in Cameroon. This study aimed to assess the IgM and IgG antibody profiles using adult S.guineensis extract in immune sera from Schistosoma egg positive Cameroonians from intestinal or urinary schistosomiasis endemic areas. Whole adult S.guineensis antigen was extracted from a Cameroonian S.guineensis strain maintained in laboratory. The antigen extract was a supernatant obtained after centrifugation of grinded freeze-thawed 45 days old worms in enzyme inhibitor solution. Sera were obtained from residents of schistosomiasis endemic areas with microscopically confirmed monospecific infection by S.guineensis, S.mansoni or S.haematobium and Schistosoma free control sera from non endemic area. IgM and IgG antibody titers detection was proceeded by indirect ELISA using HRP-conjugated secondary IgM or IgG antibody. Optical densities (OD) were recorded with a spectrophotometer plate reader at 495 nm and mean OD were statistically analyzed according to age using Student t-test and egg load using ANOVA test. A total of 189 sera were tested. Sera from S.haematobium, S.mansoni and S.guineensis infections represented 68, 73 and 46 respectively. Of S.mansoni infection sera, 31 were collected at Lagdo and 42 in S.mansoni monospecific focus. Age had no significant influence on mean IgM and IgG antibodies OD although mean IgM and IgG changed with age. Mean IgG antibody increased with age in the three schistosomiasis sera. According to age, mean IgM decreased in S.guineensis infections sera, decreased from young to participants aged 15 to 24 years then increased in older in schistosomiasis mansoni sera. In schistosomiasis haematobium sera, mean IgM increased from young to participants aged 15 to 24 years then decreased in older. Egg load influenced significantly mean IgM and IgG titers. Mean IgM OD decreased with egg load in each of the schistosomiasis whereas mean IgG decreased in intestinal schistosomiasis sera. Mean IgM and IgG antibody titers detected with adult S.guineensis extract using ELISA technique in either intestinal or urinary schistosomiasis endemic areas in Cameroon were not age dependent but egg load depended on egg load in urine and stool samples. Diagnostic performance of the adult S.guineensis extract in antibody detection has been tested.Read Complete Article at ijSciences: V82019042038 AND DOI: http://dx.doi.org/10.18483/ijSci.2038

Following highly prevalent Plasmodium resistant strains to antimalarial monotherapies in malaria endemic countries, uncomplicated malaria treatment policy changed to artemisinine-based combination therapies (ACTs). After adoption of this new treatment policy in a country, sufficient care is needed to be taken to prevent occurrence of resistance to the latest drugs. As Cameroon shifted to ACT in 2004, this study aimed to assess knowledge and practices of health workers in government health facilities of the Littoral region regarding mild malaria management in health facilities as well as according to prescription qualities of ACTs in leaflets received in pharmacies. A total of 66 physicians and 16 nurses were questioned in 10 health facilities and 503 medical leaflets with ACTs prescriptions were viewed in 17 pharmacies. All medical workers questioned correctly were defined mild malaria and were aware of the antimalarial policy change in Cameroon. Overall ACTs prescription for mild malaria management in children and adult patients was 72.2% and 87.8% respectively. An important proportion of health workers prescribed antimalarial monotherapies and non recommended antimalarial for uncomplicated malaria treatment. 31.7% of participants did not systematically recommend laboratory diagnostic test before antimalarial prescription. Of leaflets viewed in pharmacies, ACTs were prescribed by physicians, nurses and laboratory technicians. Age was the only criteria for ACTs prescription. Appropriate ACTs quality prescription ranged between 81.2% and 94.4%. Of the ACTs prescribed, blisters had the highest (92.9%) appropriate quality prescription and solutions the lowest (83.3%). According to qualification of prescribers, physicians had the highest score (93.1%) of appropriate quality prescription and laboratory technicians the lowest score (28.1%). For all ACTs containing medical leaflets, concomitant medications were recorded namely antipyretic (73.9%), antibiotic (21.9%), non steroid anti-inflammatory (19.9%) or vitamins (18.1%). Data gathered indicated that although health workers were aware of uncomplicated malaria treatment policy change in Cameroon, mild malaria mismanagement was prevailing in health facilities of the Littoral region and ACTs quality prescription in medical leaflets was not optimal. Therefore, awareness is still needed among prescribers in order to prevent or at least slow the occurrence of Plasmodium resistant strains to ACTs in Cameroon.