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Plant-parasitic nematodes (PPNs) are significant agricultural pests that can reduce maize yields. This study examines the prevalence, abundance, and diversity of PPNs in New Zealand maize fields, focusing on the effects of territory, soil orders, crop stages, and sampling times. Seven PPN genera were identified: Pratylenchus spp. (root-lesion), Helicotylenchus spp. (spiral), Meloidogyne spp. (root-knot), Heterodera spp. (cyst), Paratylenchus spp. (pin), Criconemella spp. (ring), and Tylenchus spp. PPNs were present in 98% of the samples, with Pratylenchus spp. being the most prevalent (91%), followed by Helicotylenchus spp. (38%). Compared to Waikato and Manawatu-Whanganui, Canterbury had the highest nematode populations, particularly of Pratylenchus spp. and Helicotylenchus spp. Brown and pallic soils supported higher PPN abundances. Sampling during the maize harvesting stage and late autumn resulted in the highest nematode populations and diversity indices. Pratylenchus spp. populations often exceeded the economic threshold of 500 Pratylenchus kg−1 of soil, suggesting a significant threat to maize yield in New Zealand. The findings highlight the need for further research to assess the impact of Pratylenchus spp. on maize yield and to develop effective management practices for maize cultivation in the country. [ABSTRACT FROM AUTHOR]

Bell peppers (Capsicum annuum) play a key role in food production, commerce, and society, with smallholder farmers being the 36 primary cultivators. However, the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) poses a significant threat to bell pepper crops. Traditional control methods rely heavily on the application of insecticides, resulting in increased production costs and ecological concerns. To address this issue, the establishment of decision‐making systems, starting with effective sampling plans, is crucial. This study aimed to develop a practical sampling strategy for assessing B. tabaci populations at different growth stages of bell pepper crops, including vegetative, flowering, and fruiting stages. Over a 4‐year period, commercial bell pepper fields were monitored to determine the optimal sampling technique and sample size. Results indicated that sampling the apical third of the plant's leaves and shaking the plants onto a white plastic tray yielded the most accurate samples. Pest densities followed a negative binomial distribution pattern, with a consistent aggregation parameter (Kc = 0.3339) across all fields. Therefore, assessing 78 plants per field was deemed necessary. The sampling procedure incurred a cost of up to $1.12 per hectare and required approximately 24 min. The simplicity, ease of execution, and low cost of the developed sampling strategy make it suitable for integration into comprehensive pest management programs. [ABSTRACT FROM AUTHOR]

Coffee production has become increasingly technified in order to optimize the use of inputs and the sustainable use of natural resources. In this context, one way that farmers are investing in their coffee plantations is in the use of precision agriculture techniques, termed precision coffee farming. Over the last few years, research has been conducted to facilitate the application of this technology, and sampling grids with two points per hectare have been recommended by several studies. These georeferenced demarcations in a plot are generally shaped as equidistant squares or rectangles, and the sampling points are located at the centers of these areas. Coffee farmers typically plant their crops following the level line, which greatly hinders the navigation of equidistant points within the field. Thus, the objective of this study was to develop an optimized walking route method to reduce the distance for sampling soil, leaf, and yield attributes. The experimental plots were established in 2000 at Samambaia Farm, located in Santo Antônio do Amparo, Minas Gerais, Brazil, with coffee the cultivar Acaia IAC 479-19, totaling 56.65 ha. The 111 sampling points were distributed in the land following the new method proposed in this study, and, after walking simulations using Farm Works Mapping Software, the new method was compared with the conventional method using the mean displacement between points. The new optimized walking routes method reduced the mean distance traveled to sample the points by 50.1%. [ABSTRACT FROM AUTHOR]

Coffee production has become increasingly technified in order to optimize the use of inputs and the sustainable use of natural resources. In this context, one way that farmers are investing in their coffee plantations is in the use of precision agriculture techniques, termed precision coffee farming. Over the last few years, research has been conducted to facilitate the application of this technology, and sampling grids with two points per hectare have been recommended by several studies. These georeferenced demarcations in a plot are generally shaped as equidistant squares or rectangles, and the sampling points are located at the centers of these areas. Coffee farmers typically plant their crops following the level line, which greatly hinders the navigation of equidistant points within the field. Thus, the objective of this study was to develop an optimized walking route method to reduce the distance for sampling soil, leaf, and yield attributes. The experimental plots were established in 2000 at Samambaia Farm, located in Santo Antônio do Amparo, Minas Gerais, Brazil, with coffee the cultivar Acaia IAC 479-19, totaling 56.65 ha. The 111 sampling points were distributed in the land following the new method proposed in this study, and, after walking simulations using Farm Works Mapping Software, the new method was compared with the conventional method using the mean displacement between points. The new optimized walking routes method reduced the mean distance traveled to sample the points by 50.1%.

Introduction: Cortisol secretion is regulated by circadian rhythm, which is influenced by zeitgebers like light. In India, the entire country operates under a single time zone, Indian Standard Time, which may not align with the local sunrise timing across different regions. Aims: This study aimed to compare the basal serum cortisol levels between 06:00 AM and 09:00 AM in Guwahati, Assam, where sunrise occurs earlier compared with the western part of the country. A cross-sectional pilot study was conducted from December 2022 to June 2023 in a tertiary care hospital in Guwahati. Methods: Serum cortisol samples were collected at 06:00 AM and 09:00 AM from 25 healthy adult participants once in winter and again in summer. Descriptive statistics and paired Student’s t-tests were used. Results: The mean serum cortisol levels at 06:00 AM in winter, summer and overall were 13.2, 13.4 and 13.3 μg/dL, respectively. At 09:00 AM, the mean serum cortisol levels in winter, summer and overall were 8.2, 7.7 and 8.0 μg/dL, respectively. Significant differences were observed between the 06:00 AM and 09:00 AM cortisol levels in both winter and summer (P

Abstract Background Studies suggest that cerebrospinal fluid (CSF) levels of amyloid-β (Aβ)42 and Aβ40 present a circadian rhythm. However sustained sampling of large volumes of CSF with indwelling intrathecal catheters used in most of these studies might have affected CSF dynamics and thereby confounded the observed fluctuations in the biomarker levels. Methods We included 38 individuals with either normal (N = 20) or abnormal (N = 18) CSF Aβ42/Aβ40 levels at baseline. CSF and plasma were collected at two visits separated by an average of 53 days with lumbar punctures and venipunctures performed either in the morning or evening. At the first visit, sample collection was performed in the morning for 17 participants and the order was reversed for the remaining 21 participants. CSF and plasma samples were analyzed for Alzheimer’ disease (AD) biomarkers, including Aβ42, Aβ40, GFAP, NfL p-tau181, p-tau217, p-tau231 and t-tau. CSF samples were also tested using mass spectrometry for 22 synaptic and endo-lysosomal proteins. Results CSF Aβ42 (mean difference [MD], 0.21 ng/mL; p = 0.038), CSF Aβ40 (MD, 1.85 ng/mL; p

Corn (Zea mays) is the most widely planted crop in the world. Dalbulus maidis (Hemiptera: Cicadellidae) is currently a primary corn pest. The starting point for the development of pest control decision-making systems is the determination of a conventional sampling plan. Therefore, this study aimed to determine a practical conventional sampling plan for D. maidis in corn crops. Insect density was evaluated in 28 commercial fields. Subsequently, D. maidis densities were sampled from fields ranging from 1 to 100 ha. Insect density conformed to a negative binomial distribution in 89.29% of the fields. The insect densities determined using the sampling plan had a low error rate (up to 15%). Sampling time and costs ranged from 2.06 to 39.45 min/ha and 0.09 to 1.81 USD/ha for fields of 1–100 ha, respectively. These results provide the first precise and representative conventional sampling plan for scouting D. maidis adults grown in corn fields. Therefore, the conventional sampling plan for D. maidis determined in this study is practical and can be incorporated into integrated pest management programs for corn crops owing to its representativeness, precision, speed, and low cost. [ABSTRACT FROM AUTHOR]

Background: Studies suggest that cerebrospinal fluid (CSF) levels of amyloid-β (Aβ)42 and Aβ40 present a circadian rhythm. However sustained sampling of large volumes of CSF with indwelling intrathecal catheters used in most of these studies might have affected CSF dynamics and thereby confounded the observed fluctuations in the biomarker levels. Methods: We included 38 individuals with either normal (N = 20) or abnormal (N = 18) CSF Aβ42/Aβ40 levels at baseline. CSF and plasma were collected at two visits separated by an average of 53 days with lumbar punctures and venipunctures performed either in the morning or evening. At the first visit, sample collection was performed in the morning for 17 participants and the order was reversed for the remaining 21 participants. CSF and plasma samples were analyzed for Alzheimer' disease (AD) biomarkers, including Aβ42, Aβ40, GFAP, NfL p-tau181, p-tau217, p-tau231 and t-tau. CSF samples were also tested using mass spectrometry for 22 synaptic and endo-lysosomal proteins. Results: CSF Aβ42 (mean difference [MD], 0.21 ng/mL; p = 0.038), CSF Aβ40 (MD, 1.85 ng/mL; p < 0.001), plasma Aβ42 (MD, 1.65 pg/mL; p = 0.002) and plasma Aβ40 (MD, 0.01 ng/mL, p = 0.002) were increased by 4.2-17.0% in evening compared with morning samples. Further, CSF levels of 14 synaptic and endo-lysosomal proteins, including neurogranin and neuronal pentraxin-1, were increased by 4.5-13.3% in the evening samples (MDrange, 0.02-0.56 fmol/µl; p < 0.042). However, no significant differences were found between morning and evening levels for the Aβ42/Aβ40 ratio, different p-tau variants, GFAP and NfL. There were no significant interaction between sampling time and Aβ status for any of the biomarkers, except that CSF t-tau was increased (by 5.74%) in the evening samples compared to the morning samples in Aβ-positive (MD, 16.46 ng/ml; p = 0.009) but not Aβ-negative participants (MD, 1.89 ng/ml; p = 0.47). There were no significant interactions between sampling time and order in which samples were obtained. Discussion: Our findings provide evidence for diurnal fluctuations in Aβ peptide levels, both in CSF and plasma, while CSF and plasma p-tau, GFAP and NfL were unaffected. Importantly, Aβ42/Aβ40 ratio remained unaltered, suggesting that it is more suitable for implementation in clinical workup than individual Aβ peptides. Additionally, we show that CSF levels of many synaptic and endo-lysosomal proteins presented a diurnal rhythm, implying a build-up of neuronal activity markers during the day. These results will guide the development of unified sample collection procedures to avoid effects of diurnal variation for future implementation of AD biomarkers in clinical practice and drug trials. [ABSTRACT FROM AUTHOR]

Agroforestry is known to significantly improve long-term land productivity, potentially enhancing the ability to cope with climate stress. However, there is limited information regarding the accurate monitoring of greenhouse gases (GHGs) in rubber-based agroforestry systems. Before GHGs can be accurately estimated, the diurnal variations and suitable sampling times must be studied to reduce the uncertainty of the manual static chamber method. In this study, the soil GHGs emitted from conventional single-row (SR) and improved double-row (DR) rubber plantations were compared across the dry and wet seasons in Hainan, China. A total of 1728 GHG samples from a field trial were collected, analyzed, and related to environmental factors. The results demonstrated that the diurnal fluxes of CO2 in rubber plantations were likely to remain fluctuating, with the maximum typically occurring during the night-time and daytime hours of the dry and wet seasons, respectively. A clearer double-peak (around 2:00 and 14:00) during the dry season and a daytime peak (14:00) during the wet season of the N2O were recorded. In addition to the commonalities, different seasons and different types of GHGs and rubber plantations also differed in their detailed fluctuation times and ranges; therefore, the determination of suitable sampling times should not ignore these factors in certain cases. Based on this study, it was determined that the late afternoon (16:00–18:00) was the suitable sampling time of soil GHGs in rubber plantations, instead of the most common morning times (with an underestimation of 25% on average). In addition, the air humidity during the dry season and the soil temperature during the wet season were both positively correlated with GHGs (p < 0.05). This study highlights the significance of accurately monitoring soil GHGs in rubber-based agroforestry systems, providing a basic reference for the development and management of climate-smart land use practices in rubber plantations. [ABSTRACT FROM AUTHOR]

Floorplan synthesis is the process of generating new, realistic floor plans for buildings and homes using machine learning and generative models. In recent years, various generative methods, including GANs and diffusion models, have been utilized for the task of floorplan generation, demonstrating promising advancements in architectural design and planning. However, despite their potential, these methods face unique challenges like mode collapse, training instability, and sampling time, which require innovative solutions to overcome for further progress in this field. To address these issues, various techniques such as regualrization techniques, architectural modifications, and optimization algorithms, have been employed. However, existing techniques still struggle to balance both sampling time and diversity simultaneously. In response, HouseGanDi proposes a novel hybrid approach that amalgamates GANs and diffusion models to address the dual challenges of diversity and sampling time in floorplan generation. To the best of our knowledge, this work is the first to introduce a solution that not only balances sampling time and diversity but also enhances the realism of the generated floorplans. HouseGanDi is trained on the RPLAN dataset and combines the advantages of GANs and diffusion models in multimodal fashion while incorporating different techniques such as regularization methods and architectural modifications to optimize our objectives. The multimodality allows our model to jump a number of denoising steps while capturing data distributions. To evaluate the effect of the denoising step, we experimented with different time steps and found better diversity results at T = 20. Evaluation of diversity using FID demonstrates an average 15.5% improvement over the state-of-the-art houseDiffusion model, with a 41% reduction in generation time. However, challenges persist in generating non-orthogonal floorplans and accommodating intricate spatial layouts.

Corn (Zea mays) is the most widely planted crop in the world. Dalbulus maidis (Hemiptera: Cicadellidae) is currently a primary corn pest. The starting point for the development of pest control decision-making systems is the determination of a conventional sampling plan. Therefore, this study aimed to determine a practical conventional sampling plan for D. maidis in corn crops. Insect density was evaluated in 28 commercial fields. Subsequently, D. maidis densities were sampled from fields ranging from 1 to 100 ha. Insect density conformed to a negative binomial distribution in 89.29% of the fields. The insect densities determined using the sampling plan had a low error rate (up to 15%). Sampling time and costs ranged from 2.06 to 39.45 min/ha and 0.09 to 1.81 USD/ha for fields of 1–100 ha, respectively. These results provide the first precise and representative conventional sampling plan for scouting D. maidis adults grown in corn fields. Therefore, the conventional sampling plan for D. maidis determined in this study is practical and can be incorporated into integrated pest management programs for corn crops owing to its representativeness, precision, speed, and low cost.

Shixiao Li,1 Peng Zhou,2 Lihong Yang,1 Tianbin Tang,1 Jiajia Qin,1 Jiao Qian,1 Shen Bo,1 Sufei Yu1 1Department of Clinical Microbiology Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou, Zhejiang, People’s Republic of China; 2Department of Pharmacy, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Sufei Yu, Email ysf5236791@163.comObjective: Severe pneumonia is a common infectious disease with high morbidity and mortality. Early etiological diagnosis is crucial for improving the prognosis. The aim of this study is to evaluate the clinical value of sampling time of mNGS in patients with severe pneumonia.Methods: This retrospective study enrolled 105 patients with severe pneumonia. mNGS was performed on bronchoalveolar lavage fluid (BALF). Patients were divided into the sampling time ≤ 72h vs sampling time > 72h groups and survivors vs non-survivors groups according to their sampling time and prognosis. Clinical characteristics, the adjustment of antibiotics and clinical prognostic value were evaluated.Results: Our study showed that, early sampling of mNGS can significantly shorten the mechanical ventilation time (p = 0.007) and hospitalization time (p = 0.004). In the non-survivors group, CURB-65, SOFA, and APACHE II scores were higher. Age (OR: 1.051, 95% CI: 1.004– 1.100, p = 0.034), chronic respiratory diseases (OR: 4.639, 95% CI: 1.260– 17.082, p = 0.021), immunosuppression (OR: 5.008, 95% CI: 1.617– 15.510, p = 0.005) and SOFA score on the day of mNGS sampling (OR: 1.492, 95% CI: 1.212– 1.837, p < 0.001) were independent risk factors of in-hospital mortality. The most common pathogens were Klebsiella pneumoniae and Human gammaherpesvirus 4. The proportion of appropriate and targeted antibiotics adjusted was significantly higher than that in the sampling time > 72h group, and the proportion of antifungal and antiviral agents adjusted was lower. In the early sampling group, it was significantly decreased in the CRP, PCT level and NEU% at discharge.Conclusion: This study demonstrated that early sampling of mNGS could shorten the time of mechanical ventilation and hospitalization of patients with severe pneumonia. Patients with higher SOFA score on the day of sampling had a poorer prognosis. It emphasizes that early sampling of mNGS has a positive value.Keywords: metagenomic next generation sequencing, severe pneumonia, sampling time, mortality

In this paper, a switching frequency has been formulated for grid-connected voltage source inverter (VSI) operated using the Continuous Control Set-Predictive Current Control (CCS-PCC) technique. An instantaneous expression of switching frequency has been derived for the VSI operated using the predictive current control technique. The effect of sampling frequency has been analyzed on the switching frequency of the converter operation. The effect of sampling time has a direct impact on the switching frequency as the cost function of the PCC depends on the sampling frequency. The analysis provides a generalized mathematical expression of switching frequency for the VSI operating in current control mode using the predictive current control method. With the help of this generalized expression, the maximum switching frequency and minimum load current error have been obtained. Simulation has been performed for verification of the analytical results through a Typhoon HIL-402 kit for real-time simulation and experimental validation. [ABSTRACT FROM AUTHOR]

Iodine deficiency may cause thyroid dysfunction. The iodine intake in a population is measured by urinary iodine concentration (UIC) in spot samples or 24-h urinary iodine excretion (24UIE). 24UIE is considered the gold standard and may be estimated using an equation including UIC, urinary creatinine concentration, sex and age (e24UIE). The aims of this study were to evaluate the preferable timing of UIC when using this equation and assess the variability of UIE. Sixty healthy non-smoking women (n 31) and men (n 29) were included in Gothenburg, Sweden. Twelve urine samples were collected at six fixed times on two separate days. Variability was calculated for UIC, 24UIE, e24UIE, iodine excretion per hour (iHr) and UIC adjusted for creatinine and specific gravity. Median 24UIE was 156 µg/24 h and the median UIC (all spot samples) was 104 µg/l. UIC (P < 0·001), 24UIE (P = 0·001) and e24UIE (P < 0·001) were significantly higher in men. e24UIE was relatively similar to 24UIE. However, when e24UIE was calculated from UIC in the first void, it was about 15 % lower than 24UIE (P < 0·001). iHr was lowest in the morning and highest in the afternoon. Median iHr was higher in men (7·4 v. 5·3 µg/h, P < 0·001). The variability of UIE was higher within individuals than between individuals. This study suggests that most time points for estimation of individual 24UIE are appropriate, but they should preferably not be collected in the first void. [ABSTRACT FROM AUTHOR]

The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue techniques is fundamental. In this research, a grape embryo-culture protocol was optimized and validated by using 39 cultivars and 41 cross-combinations carried out in the framework of a large private table grape program of the private network Italian Variety Club in the period 2017–2021 evaluating several factors, such as the improvement in embryo formation, germination and growth, and plantlet development. The embryo culture attitude of crosses between different combinations of seedless parents was assessed, and the rates of embryo development from the extracted ovules mostly ranged from 3.5 to 35.5% with 5 out of 43 genotypes outliers. Experiments conducted at different sampling times, in a range of 43–62 days after pollination (DAP), did not show significant differences between the samples analyzed, while the rate of embryos developed with the applied protocol proved its employability on multiple genotypes, although the grapevine genotype significantly influenced the technique efficiency. [ABSTRACT FROM AUTHOR]

Agroforestry is known to significantly improve long-term land productivity, potentially enhancing the ability to cope with climate stress. However, there is limited information regarding the accurate monitoring of greenhouse gases (GHGs) in rubber-based agroforestry systems. Before GHGs can be accurately estimated, the diurnal variations and suitable sampling times must be studied to reduce the uncertainty of the manual static chamber method. In this study, the soil GHGs emitted from conventional single-row (SR) and improved double-row (DR) rubber plantations were compared across the dry and wet seasons in Hainan, China. A total of 1728 GHG samples from a field trial were collected, analyzed, and related to environmental factors. The results demonstrated that the diurnal fluxes of CO2 in rubber plantations were likely to remain fluctuating, with the maximum typically occurring during the night-time and daytime hours of the dry and wet seasons, respectively. A clearer double-peak (around 2:00 and 14:00) during the dry season and a daytime peak (14:00) during the wet season of the N2O were recorded. In addition to the commonalities, different seasons and different types of GHGs and rubber plantations also differed in their detailed fluctuation times and ranges; therefore, the determination of suitable sampling times should not ignore these factors in certain cases. Based on this study, it was determined that the late afternoon (16:00–18:00) was the suitable sampling time of soil GHGs in rubber plantations, instead of the most common morning times (with an underestimation of 25% on average). In addition, the air humidity during the dry season and the soil temperature during the wet season were both positively correlated with GHGs (p < 0.05). This study highlights the significance of accurately monitoring soil GHGs in rubber-based agroforestry systems, providing a basic reference for the development and management of climate-smart land use practices in rubber plantations.

Maize (Zea mays) is the most consumed food in the world. The leafhopper, Dalbulus maidis, (Hemiptera: Cicadellidae) is one of the most important maize pests, and due to its direct and indirect damage, it can cause losses of up to 100%. Sampling plans are essential components for integrated pest management programs. The first two components to be determined in these plans are the sampling unit and the sampling technique. The sampling unit consists of determining the plant organ to be evaluated in the sampling and the techniques are the devices used to sample populations of pests in crops. Thus, the objective of this work was to select the unit and technique to be used in the sampling of D. maidis in maize crops. For this purpose, pest populations in plants at stages V4, V6, V8, and V10 were monitored in 16 commercial crops in the Atlantic Forest and Cerrado biomes over two years. The sample units studied were the leaves at different positions in the canopy of the plants. The techniques studied were direct counting, shaking the apex of the plants in a plastic tray and using a beating cloth. In the process of selecting the unit and technique to be used in sampling populations of pests in crops, adequate criteria must be used. These criteria include precision, representativeness, sampling time and that the use of these components in the sampling plans should not cause damage to the plants. The most suitable sampling unit for evaluating these pest populations was the whorl leaves and the best technique was the direct counting. Therefore, the unit and technique selected in this work can be used in sampling plans for D. maidis in maize crops. [ABSTRACT FROM AUTHOR]

We examined the blood lactate response, in terms of the maximal post-exercise concentration (Lamax), time to reach Lamax, and maximal lactate accumulation rate (VLamax), to swimming sprints of 25, 35, and 50 m. A total of 14 highly trained and elite swimmers (8 male and 6 female), aged 14–32, completed the 3 sprints in their specialization stroke with 30 min of passive rest in between. The blood lactate was measured right before and continually (every minute) after each sprint to detect the Lamax. The VLamax, a potential index of anaerobic lactic power, was calculated. The blood lactate concentration, swimming speed, and VLamax differed between the sprints (p < 0.001). The Lamax was highest after 50 m (13.8 ± 2.6 mmol·L–1, mean ± SD throughout), while the swimming speed and VLamax were highest at 25 m (2.16 ± 0.25 m·s–1 and 0.75 ± 0.18 mmol·L–1·s–1). The lactate peaked approximately 2 min after all the sprints. The VLamax in each sprint correlated positively with the speed and with each other. In conclusion, the correlation of the swimming speed with the VLamax suggests that the VLamax is an index of anaerobic lactic power and that it is possible to improve performance by augmenting the VLamax through appropriate training. To accurately measure the Lamax and, hence, the VLamax, we recommend starting blood sampling one minute after exercise. [ABSTRACT FROM AUTHOR]

Whether environmental DNA (eDNA) testing methods are effective for semi-aquatic organisms that spend time both in rivers and on land remains unclear. We designed a species-specific primer–probe set for the semi-aquatic Japanese water shrew, Chimarrogale platycephala, an endangered top predator species for which limited ecological information is available. The designed primer–probe set was used to determine the effective sampling time for detecting eDNA from this species in running water. Hourly water sampling was conducted for 25 h in the autumn of 2020. Water sampling and camera trapping were conducted in the two streams in which feces had been observed on stream rocks. No C. platycephala activity was captured by the camera traps at any sampling point during the investigation. However, eDNA was mainly detected at night in both streams and twice during the daytime in one stream; the eDNA detection periods differed between streams (21:00–23:00 vs. 1:00–8:00 and daytime). These results demonstrate that eDNA testing methods are effective for detecting small semi-aquatic mammals. However, the sampling time affected the results, and eDNA was mostly detected at night, possibly because of the nocturnal activity of the species. Therefore, the sampling time should be adjusted to match the activity period of a species to improve the efficiency of eDNA testing for semi-aquatic organisms. [ABSTRACT FROM AUTHOR]

Abstract Background In eye-drop drug development, the additional genotoxicity tests in some cases might be necessary to assess genotoxicity in the ocular surface since the ocular surface is exposed directly to high drug concentrations. Recently, an in vivo comet assay using corneal epithelial cells in rabbits following single ocular instillation was developed as an assay to evaluate genotoxicity in ocular tissues. In this study, we investigated the time-course changes in DNA damage after ocular instillation of genotoxic compounds to evaluate the optimal sampling timing for in vivo comet assay of the ocular surface tissue. Ethidium bromide (EtBr), methyl methanesulfonate (MMS), and 4-nitroquinoline 1-oxide (4-NQO) were administered to the eyes of the rabbits. Corneas were collected at 0.5, 2, 4, 6, and 24 h after administration, and the comet assay was performed. In addition, the in vitro comet assay was performed to assess the time-course changes in DNA damage induced by short-time exposure to the genotoxic compounds. Results The mean % tail DNA, which is an indicator for DNA damage, in the corneal epithelial cells treated with all compounds exhibited statistically significant increases compared with those in the negative controls of saline at 0.5, 2, 4, and 6 h. There was a difference in the DNA damage response between EtBr and the other two compounds. In the 3% MMS- and 1% 4-NQO-treated eyes, the values of the % tail DNA were the highest at 0.5 h and then decreased gradually. In contrast, in the 1% EtBr-treated eyes, the highest value was noted at 4 h. The results of the in vitro comet assay showed that the % tail DNA increased in all groups. A further increase in the % tail DNA occurred in the EtBr-treated cells even after removing the compound but not in the MMS- and 4-NQO-treated cells. Conclusion Relatively high values of the % tail DNA were maintained from 0.5 to 6 h after administration, suggesting that the optimal sampling time is any one point from 0.5 to 6 h in the comet assay of the corneal surface.

Abstract Background This study aimed to investigate the effects of blood sampling after calorie intake on thyroid stimulating hormone (TSH) level, compared with blood sampling in fasting state. Methods This was a prospective, randomized, controlled study. Subjects from the outpatients in the department of endocrinology without evidence of thyroid diseases were included and then randomized into the fasting group, diet intake group, and glucose intake group, respectively. Fasting blood was collected from all subjects at 7:00 am for the measurement of TSH and free thyroxine (FT4) concentrations. Afterwards, the subjects were maintained at fasting state (fasting group), had an intake of the mixed diet with 400 kcal calories (diet intake group), and had an intake of 75 g glucose (glucose intake group), respectively, and blood was collected again 2 h later (9:00 am on the same day) for TSH and FT4 level measurement and comparison. Results A total of 150 subjects were enrolled, of whom 146 met the inclusion criteria, with 48, 48, and 50 in the diet intake group, glucose intake group, and fasting group, respectively. The TSH in the diet intake group was significantly lower at 9:00 am (TSH9am) than the level at 7:00 am (TSH7am) (P

The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue techniques is fundamental. In this research, a grape embryo-culture protocol was optimized and validated by using 39 cultivars and 41 cross-combinations carried out in the framework of a large private table grape program of the private network Italian Variety Club in the period 2017–2021 evaluating several factors, such as the improvement in embryo formation, germination and growth, and plantlet development. The embryo culture attitude of crosses between different combinations of seedless parents was assessed, and the rates of embryo development from the extracted ovules mostly ranged from 3.5 to 35.5% with 5 out of 43 genotypes outliers. Experiments conducted at different sampling times, in a range of 43–62 days after pollination (DAP), did not show significant differences between the samples analyzed, while the rate of embryos developed with the applied protocol proved its employability on multiple genotypes, although the grapevine genotype significantly influenced the technique efficiency.

This paper presents the research platform for real time digital simulation applications which replaces the requirement of full scale or partial scale validation of physical systems. To illustrate this, a three phase AC-DC-AC converter topology has been used with diode rectifier, dc link and ideal two level inverter with inductive load. Because of natural decoupling between rectifier and inverter, it is easy to solve the state equation with reduced order system using decoupled method. This method utilizes analytical to formulate the state equations and to rectify the zero crossing errors Taylor's method of interpolation has been implemented which leads to variable step time during that instant otherwise fixed step size of 100 µs is used. The proposed algorithm has been validated in simulation and real time controller domain. Performance of the converter is evaluated based on accuracy, zero crossing and tabulated. [ABSTRACT FROM AUTHOR]

In this article, an event-triggered technique is proposed to implement state feedback controllers for continuous-time linear systems. Toward this end, the closed-loop dynamic is decomposed into the fast and slow subsystems. Then, two individualized event-triggered mechanisms (ETMs) are designed to compute two sequences of updates. The first ETM determines when the fast subsystem should be sampled and its measured data should be sent to the controller. The second ETM decides the execution times for sampling all the system states, including both the fast and slow states. The proposed event-triggered technique leads to sampling policies in which the data of the slow states are sent to the controller with long interevent intervals whereas the fast dynamics are simultaneously allowed to be sampled with relatively shorter interevent intervals. In comparison with conventional methods with one ETM, the proposed event-triggered technique, equipped with an extra degree of freedom, leads to better results both in achieving smoother closed-loop responses and reducing the number of the transmitted packages from the system to the controller. Theoretical aspects of the proposed method including the boundedness of the system and its Zeno free property are investigated. Two simulation and experimental case studies are provided to show the effectiveness of the proposed event-triggered controller. [ABSTRACT FROM AUTHOR]

Extracted driving behavior of human driven vehicles can benefit the development of various applications like trajectory prediction or planning, abnormal driving detection, driving behavior classification, traffic simulation modeling, etc. In this paper, we focus on modeling human driving behavior in order to find simplifications for trajectory planning. Using a time-discrete kinematic bicycle model with the vehicle’s acceleration and steering rate as inputs, we model the human driven trajectories of an urban intersection drone dataset for different input sampling times. While most planning algorithms are using input sampling times below 0.33s, we are able to model 98.2% of the human driven trajectories of the investigated dataset with a sampling time of 0.6s. Using longer input sampling times can result in smoother trajectories and longer planning horizons, and thus more efficient trajectories. In a next step, we analyze the correlations between the input of our model and the current state/last input. Such a priori knowledge could simplify common planning algorithms like model predictive control or tree-search based planners by limiting the action space of the ego-vehicle. We propose nonlinear transformations for steering rate and steering angle to represent correlations between speed, acceleration, steering angle and steering rate. In the transformed space the statistics are very well modeled by multivariate Gaussian distributions. Using a multivariate Gaussian, a fast usable behavior model is extracted which is independent of the environment. [ABSTRACT FROM AUTHOR]

This paper proposes a method for selecting the parameters of the main components of an autonomous photovoltaic (PV) stations to ensure the most efficient conversion and use of solar energy. The main energy characteristics are analyzed and the mathematical models of the components of an autonomous PV system are developed to study the modes of tracking the maximum power point (MPP). The necessary conditions for matching the parameters of the PV and the DC–DC buck converter are evaluated to track the MPP. This paper aims to create an original method and algorithm for calculating and selecting parameters of the main elements of a PV stations. The optimal value of the sampling time of the MPP controller is obtained for different schemes of the PV system . A practical example is considered for selecting the parameters of a DC–DC buck converter and a digital MPP of an autonomous PV station. The simulation of dynamic modes of an autonomous photovoltaic (PV) station is performed in the MATLAB/SIMULINK software package. The simulation results of dynamic operations of the PV system show that the voltage converter and the MPP controller with the selected parameters according to the proposed method provide reliable and effective tracking of the MPP in all different connections of the PV.

Fungi play a fundamental role in the intestinal ecosystem and health, but our knowledge of fungal composition and distribution in the whole gastrointestinal tract (GIT) is very limited. The physiological similarity between humans and pigs in terms of digestive and associated metabolic processes places, the pig in a superior position over other non-primate models. Here, we aimed to characterize the diversity and composition of fungi in the GIT of pigs. Using high-throughput sequencing, we evaluated the fungal community in different locations of GIT of 11 pigs with 128.41 ± 1.25 kg body weight acquired successively. Among them, five pigs are sacrificed in April 2019 (Batch 1) and the other six are sacrificed in January 2020 (Batch 2). All subjects with similar genetic backgrounds, housing, management, and diet. Finally, no significant difference is found in the α-diversity (Richness) of the fungal community among all intestinal segments. Basidiomycota and Ascomycota are the two predominant fungal phyla, but Batch 1 harbored a notably high abundance of Basidiomycota and Batch 2 harbored a high abundance of Ascomycota. Moreover, the two batches harbored completely different fungal compositions and core fungal genera. FUNGuild (Fungal Functional Guild) analysis revealed that most of the fungal species present in the GIT are saprotroph, plant pathogen, and animal endosymbiont. Our study is the first to report that even under the same condition, large variations in fungal composition in the host GIT still occur from batch-to-batch and sampling time. The implications of our observations serve as references to the development of better models of the human gut.

Maize (Zea mays) is the most consumed food in the world. The leafhopper, Dalbulus maidis, (Hemiptera: Cicadellidae) is one of the most important maize pests, and due to its direct and indirect damage, it can cause losses of up to 100%. Sampling plans are essential components for integrated pest management programs. The first two components to be determined in these plans are the sampling unit and the sampling technique. The sampling unit consists of determining the plant organ to be evaluated in the sampling and the techniques are the devices used to sample populations of pests in crops. Thus, the objective of this work was to select the unit and technique to be used in the sampling of D. maidis in maize crops. For this purpose, pest populations in plants at stages V4, V6, V8, and V10 were monitored in 16 commercial crops in the Atlantic Forest and Cerrado biomes over two years. The sample units studied were the leaves at different positions in the canopy of the plants. The techniques studied were direct counting, shaking the apex of the plants in a plastic tray and using a beating cloth. In the process of selecting the unit and technique to be used in sampling populations of pests in crops, adequate criteria must be used. These criteria include precision, representativeness, sampling time and that the use of these components in the sampling plans should not cause damage to the plants. The most suitable sampling unit for evaluating these pest populations was the whorl leaves and the best technique was the direct counting. Therefore, the unit and technique selected in this work can be used in sampling plans for D. maidis in maize crops.

Improving production efficiency while enhancing pork quality is pivotal for strengthening sustainable pork production. Being able to study both gene expression and indices of pork quality from the same anatomical location of an individual animal would better enable research conducted to study relationships between animal growth and carcass merit. To facilitate gene expression studies, adipose and muscle tissue samples are often collected immediately following exsanguination to maximize RNA integrity, which is a primary determinant of the sensitivity of RNA-based assays, such as real-time PCR. However, collecting soft tissue samples requires cutting through the hide or skin. This leaves the underlying tissue exposed during scalding, poses possible food safety issues, and potentially confounds pork quality measures. To overcome these limitations, the effect of tissue sample timing post-harvest on RNA integrity, real-time PCR results, and pork quality measurements was investigated by sampling subcutaneous adipose tissue and longissimus thoracis et lumborum muscle immediately following either exsanguination, scalding, or chilling. Sampling time did not affect RNA quality, as determined by the RNA integrity number of RNA samples purified from either adipose (RIN; p > 0.54) or muscle tissue (p > 0.43). Likewise, the sampling time did not influence the results of real-time PCR analysis of gene expression when comparing RNA samples prepared from adipose or muscle tissue immediately following either exsanguination or scalding (p > 0.92). However, sampling tissue prior to scalding resulted in a greater visual color score (p < 0.001) and lesser L* (p < 0.001) and b* (p < 0.001) values without impacting the 24 h pH (p < 0.41). These results suggested that if both RNA-based assays and meat quality endpoints are to be performed at the same anatomical location on an animal, tissue sampling to facilitate RNA-based assays should occur at a time point immediately following scalding. These findings demonstrated that sampling of adipose and muscle tissue can be delayed until after scalding/dehairing without decreasing the RNA integrity or altering the results of real-time PCR assays, while doing so was associated with little impact on measures of pork quality. [ABSTRACT FROM AUTHOR]

Abstract Background To analyze the characteristics of basal thyroid hormone levels in infertile women consulting for assisted reproductive technology (ART) treatment. Methods This was a retrospective study. Serum TSH, FT3 and FT4 levels of women seeking ART consultation were tested routinely. Analyses were performed based on age and sampling time. One-way ANOVA or Kruskal-Wallis rank sum test was used to compare the continuous data among the groups, and the chi-square test or Fisher’s exact test was used to compare categorical data where appropriate. Results A total of 6426 women were initially included in the study. After exclusion criteria were applied, the remaining 4126 women were categorized into different groups. The prevalence of subclinical hypothyroidism significantly decreased with age and sampling time, from 21.09 to 11.91% and from 28.57 to 10.67%, respectively (P

Vancomycindosing error and inappropriate monitoring is a common problem in hospital daily practice. In King Abdulaziz Medical City (KAMC) in Jeddah, a high percentage of abnormal vancomycin trough levels is still detected despite using the recommended dose. Therefore, the current research objective is to study the major causes of vancomycin dosing errors. This retrospective, single-center, cross-sectional study was carried out at KAMC hospital in Jeddah from January 1st until December 31st 2019. All adult patients (≥15 years) who received vancomycin and had an initial abnormal trough level at the measured steady-state were included in this study. 472 patients have met the study inclusion criteria.The current study evaluated the factors that play a role in causing vancomycin trough level abnormalities such as sampling time, vancomycin dosing, and patient’s pharmacokinetic and pharmacodynamic variations.In this study, we found that pharmacokinetic and pharmacodynamic variability was attributed to 65% of vancomycin's abnormal trough level. Also, the result showed a significantly increased odds of the low trough in the non-elderly group (OR 6, 95% CI 2.48 – 14.9, P 0.05). The current study indicated that vancomycin dosing errors were common in KAMC patients; thus, there is an unmet need to evaluate the causes of vancomycin abnormal trough level and optimize a strategy that would enhance the therapeutic effectiveness and minimize the potential toxicity.

To investigate the effect of some cover crops incontroling weed, two experiments were conducted during the fall of 2018 at Namin in Ardabil province both with three replications.The first experiment was conducted in a randomized complete block design using four speacies of winter cover crops consisting of Rye (Secale cereal L.), Hairy vetch (Vicia villosa Roth), Chickling pea (Lathyrus sativa L.) and Hungarian vetch (Vicia panonica L.). The second experiment was conducted in a factorial design, based on randomized complete blocks with two sampling times: (before the end of cover crops growth (when the cover crops were green) and 40 days after the end of cover crops growth (when the cover crops were not green). The results showed that the highest total weed density was related to Hairy vetch and Chickling pea treatments (30.5 and 27 plant per square meter, respectively) and lowest weed density to Rye and Hungarian vetch treatments (17.5 and 22 plant per square meter) respectively. The results also showed that the highest total weed density was recooded from the second sampling time (30.33 plant per square meter) and the lowest total weed density (18.16 plant per square meter) was obtained from the first time of sampling. The highest levels of Shannon-Wiener and Simpson indices were related to the clusters of Hairy vetch, Chickling pea and Hungarian vetch. Rye and Hungarian vetch cover crops, and the first sampling time (presence of green cover crops) were the most suitable types of cover crops and sampling times to reduce weed density. It can be inferred that there is a parallel relationship between plant biomass (establishment, overwintering and regrowth of cover crops) and weed density and dry biomass. Thus, with increasing biomass of cover crops, the density and biomass of weeds would decrease.

There is a renewed global awareness to improve soil health through the intensification and management of organic inputs such as the application of animal waste–based digestate and other types of organic fertilizers to the soil. The objective of this study was to evaluate the influence of different types of animal waste–based digestate application on soil prokaryotic diversity and composition in an agricultural cropping system over a period of 3 years, cultivated with three different annual cereal crops (spring wheat, triticale, and barley). Treatments were laid out in a randomized design with five conditions (three replicates per condition): fertilizer treatments included three different types of digestate (pig manure, chicken manure, and cow manure digestates), synthetic mineral nitrogen, and unfertilized control. Prokaryotic soil communities were characterized by Illumina MiSeq sequencing. The three most abundant phyla identified were Actinobacteria, Acidobacteria, and Proteobacteria, which accounted for over 55% of the total prokaryotic community. Other phylogenetic groups such as Verrucomicrobia and Bacteroidetes were also identified as part of the native soil microbiota. It was observed that the period of digestate application did not significantly influence the prokaryotic diversity in the soil. On the contrary, sampling time was a major factor in driving β-diversity. A correlation with soil pH was also observed for several taxonomic groups, indicating its importance in shaping prokaryotic community composition. Our study showed that the richness and diversity of the soil prokaryotic community were not affected by digestate application, while other factors such as the yearly crop varieties and seasonal/climate changes were the major contributors to differentiating the prokaryotic community composition over time.

We examined the blood lactate response, in terms of the maximal post-exercise concentration (Lamax), time to reach Lamax, and maximal lactate accumulation rate (VLamax), to swimming sprints of 25, 35, and 50 m. A total of 14 highly trained and elite swimmers (8 male and 6 female), aged 14–32, completed the 3 sprints in their specialization stroke with 30 min of passive rest in between. The blood lactate was measured right before and continually (every minute) after each sprint to detect the Lamax. The VLamax, a potential index of anaerobic lactic power, was calculated. The blood lactate concentration, swimming speed, and VLamax differed between the sprints (p < 0.001). The Lamax was highest after 50 m (13.8 ± 2.6 mmol·L–1, mean ± SD throughout), while the swimming speed and VLamax were highest at 25 m (2.16 ± 0.25 m·s–1 and 0.75 ± 0.18 mmol·L–1·s–1). The lactate peaked approximately 2 min after all the sprints. The VLamax in each sprint correlated positively with the speed and with each other. In conclusion, the correlation of the swimming speed with the VLamax suggests that the VLamax is an index of anaerobic lactic power and that it is possible to improve performance by augmenting the VLamax through appropriate training. To accurately measure the Lamax and, hence, the VLamax, we recommend starting blood sampling one minute after exercise.

Background: In eye-drop drug development, the additional genotoxicity tests in some cases might be necessary to assess genotoxicity in the ocular surface since the ocular surface is exposed directly to high drug concentrations. Recently, an in vivo comet assay using corneal epithelial cells in rabbits following single ocular instillation was developed as an assay to evaluate genotoxicity in ocular tissues. In this study, we investigated the time-course changes in DNA damage after ocular instillation of genotoxic compounds to evaluate the optimal sampling timing for in vivo comet assay of the ocular surface tissue. Ethidium bromide (EtBr), methyl methanesulfonate (MMS), and 4-nitroquinoline 1-oxide (4-NQO) were administered to the eyes of the rabbits. Corneas were collected at 0.5, 2, 4, 6, and 24 h after administration, and the comet assay was performed. In addition, the in vitro comet assay was performed to assess the time-course changes in DNA damage induced by short-time exposure to the genotoxic compounds. Results: The mean % tail DNA, which is an indicator for DNA damage, in the corneal epithelial cells treated with all compounds exhibited statistically significant increases compared with those in the negative controls of saline at 0.5, 2, 4, and 6 h. There was a difference in the DNA damage response between EtBr and the other two compounds. In the 3% MMS- and 1% 4-NQO-treated eyes, the values of the % tail DNA were the highest at 0.5 h and then decreased gradually. In contrast, in the 1% EtBr-treated eyes, the highest value was noted at 4 h. The results of the in vitro comet assay showed that the % tail DNA increased in all groups. A further increase in the % tail DNA occurred in the EtBr-treated cells even after removing the compound but not in the MMS- and 4-NQO-treated cells. Conclusion: Relatively high values of the % tail DNA were maintained from 0.5 to 6 h after administration, suggesting that the optimal sampling time is any one point from 0.5 to 6 h in the comet assay of the corneal surface. [ABSTRACT FROM AUTHOR]

Regular water quality monitoring is required for protecting the physiochemical and bacteriological quality of surface water and to ensure water is meeting beneficial use criteria. This study investigated the effect of the time of sample collection on bacterial abundance and physicochemical parameters in an urban stream influenced by wastewater effluent but no rain events over 5 days on 3-h intervals. Temperature, discharge, pH, and conductivity were monitored onsite and they varied significantly (P<0.05) hourly. Total coliform and E. coli were measured in the stream water, sediments, and the treatment plant effluent. Over a day, microbial abundance varied eightfold to 21-fold upstream and twofold to 22-fold downstream of the discharge point for total coliform and E. coli, respectively. Compared with the regulatory 206 colony forming units (CFU)/100 mL coliform limit, stream samples exceeded safe limits in 70% of observations at 08:00, 50% at 11:00 and 14:00, and 10% at 17:00. Sediment samples from the upstream and downstream locations showed no significant variation in the microbial abundance based on time of sample collection, although the wastewater effluent effect was clearly seen. These results suggest repeated sampling of streams should be conducted at the same time of day to accurately inform risk-based sampling plans. [ABSTRACT FROM AUTHOR]

However, we acknowledge that longer duration recordings (e.g., 20 min) may provide better characterization of sympathetic transduction patterns in individuals with lower resting heart rates and/or burst incidence (e.g., young female adults). Sympathetic nervous system, Sympathetic transduction, Sampling time, Neurovascular transduction Keywords: Sympathetic transduction; Sympathetic nervous system; Sampling time; Neurovascular transduction EN Sympathetic transduction Sympathetic nervous system Sampling time Neurovascular transduction 155 158 4 05/05/22 20220401 NES 220401 Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s10286-022-00861-6. [Extracted from the article]

Background: This study aimed to investigate the effects of blood sampling after calorie intake on thyroid stimulating hormone (TSH) level, compared with blood sampling in fasting state. Methods: This was a prospective, randomized, controlled study. Subjects from the outpatients in the department of endocrinology without evidence of thyroid diseases were included and then randomized into the fasting group, diet intake group, and glucose intake group, respectively. Fasting blood was collected from all subjects at 7:00 am for the measurement of TSH and free thyroxine (FT4) concentrations. Afterwards, the subjects were maintained at fasting state (fasting group), had an intake of the mixed diet with 400 kcal calories (diet intake group), and had an intake of 75 g glucose (glucose intake group), respectively, and blood was collected again 2 h later (9:00 am on the same day) for TSH and FT4 level measurement and comparison. Results: A total of 150 subjects were enrolled, of whom 146 met the inclusion criteria, with 48, 48, and 50 in the diet intake group, glucose intake group, and fasting group, respectively. The TSH in the diet intake group was significantly lower at 9:00 am (TSH9am) than the level at 7:00 am (TSH7am) (P < 0.001), with a median variation of -0.71 mU/L, and a median variation rate of -32.4%. In the glucose intake group, TSH9am was also significantly lower than TSH7am (P < 0.001), with a median variation of -0.73 mU/L, and a median variation rate of -31.5%. For the fasting group, TSH9am decreased slightly but was significantly lower than TSH7am (P < 0.001), with a median variation of -0.1 mU/L, and a median variation rate of -5.2%. According to TSH7am measurements, 9 subjects (3 subjects in each group) met the diagnostic criteria of subclinical hypothyroidism. However, according toTSH9am measurements, only 2 patients in the fasting group met the diagnostic criteria of subclinical hypothyroidism. Conclusion: Compared with the fasting state, the TSH level at 2 h after the calorie intake was decreased by about 30%, which might influence the diagnosis of subclinical hypothyroidism. Trial registration: ChiCTR2100047454 (18/06/2021). [ABSTRACT FROM AUTHOR]

Background: Tacrolimus (TAC) is an important immunosuppressant for children with primary nephrotic syndrome (PNS). The relationship between sampling time variability in TAC therapeutic drug monitoring and dosage regimen in such children is unknown. Methods: In this single-center, prospective, observational study, we evaluated the sampling time variability, concentration error (CE), relative CE (RCE), and the impact of the sampling time on TAC dosage regimens in 112 PNS children with 188 blood samples. Nominal concentration (Cnom) at 12-h after last TAC dose was simulated based on observed concentration (Cobs) via previously published pharmacokinetic models, then CE and RCE were calculated. Inappropriate dosing adjustments resulting from deviated sampling time were evaluated based on a target Cnom of 5–10 ng/ml. Results: We found that 32 and 68% of samples were respectively collected early (2–180 min) and delayed (4–315 min). Furthermore, 24, 22, 22, and 32% of blood samples were drawn within deviations of ≤0.5, 0.5–1, 1–2, and >2 h, respectively, and 0.3 ng/ml of CE and 6% RCE per hour of deviation occurred. Within a deviation of >2 h, 25% of Cobs might result in inappropriate dosing adjustments. Early and delayed sampling might result in inappropriate dose holding or unnecessary dose increments, respectively, in patients with Cobs ∼ 5 ng/ml. Conclusions: Variable sampling time might lead to inappropriate dosing adjustment in a minority of children with PNS, particularly those with TAC Cobs ∼ 5 ng/ml collected with a deviation of >2 h. [ABSTRACT FROM AUTHOR]

This paper proposes a nonuniform delay-dependent robust secondary voltage control strategy with a finite-time voltage reference observer for an islanded microgrid. A discrete-time consensus algorithm is introduced to track the output voltage. By model transformation, a closed-loop microgrid control system is obtained. And then, the corresponding global stability and robust performance analysis theorems are derived. After implementing a series of trial studies, the impacts of secondary voltage control gains, sampling time on energy-to-peak robust performance are obtained. And the feasible optimal voltage control gains can be derived. Besides, a distributed cooperative secondary frequency control is introduced. Simulations of a four inverter-based microgrid test system are presented to verify the control performance. The simulation results indicate that our proposed method can maintain accurate voltage/frequency restoration and active power sharing. In addition, the voltage control gain parameters, arbitrary nonuniform time delays, and especially sampling time effects on the secondary voltage control performance are also verified. [ABSTRACT FROM AUTHOR]

Background: Tacrolimus (TAC) is an important immunosuppressant for children with primary nephrotic syndrome (PNS). The relationship between sampling time variability in TAC therapeutic drug monitoring and dosage regimen in such children is unknown.Methods: In this single-center, prospective, observational study, we evaluated the sampling time variability, concentration error (CE), relative CE (RCE), and the impact of the sampling time on TAC dosage regimens in 112 PNS children with 188 blood samples. Nominal concentration (Cnom) at 12-h after last TAC dose was simulated based on observed concentration (Cobs) via previously published pharmacokinetic models, then CE and RCE were calculated. Inappropriate dosing adjustments resulting from deviated sampling time were evaluated based on a target Cnom of 5–10 ng/ml.Results: We found that 32 and 68% of samples were respectively collected early (2–180 min) and delayed (4–315 min). Furthermore, 24, 22, 22, and 32% of blood samples were drawn within deviations of ≤0.5, 0.5–1, 1–2, and >2 h, respectively, and 0.3 ng/ml of CE and 6% RCE per hour of deviation occurred. Within a deviation of >2 h, 25% of Cobs might result in inappropriate dosing adjustments. Early and delayed sampling might result in inappropriate dose holding or unnecessary dose increments, respectively, in patients with Cobs ∼ 5 ng/ml.Conclusions: Variable sampling time might lead to inappropriate dosing adjustment in a minority of children with PNS, particularly those with TAC Cobs ∼ 5 ng/ml collected with a deviation of >2 h.

Background: To analyze the characteristics of basal thyroid hormone levels in infertile women consulting for assisted reproductive technology (ART) treatment.Methods: This was a retrospective study. Serum TSH, FT3 and FT4 levels of women seeking ART consultation were tested routinely. Analyses were performed based on age and sampling time. One-way ANOVA or Kruskal-Wallis rank sum test was used to compare the continuous data among the groups, and the chi-square test or Fisher's exact test was used to compare categorical data where appropriate.Results: A total of 6426 women were initially included in the study. After exclusion criteria were applied, the remaining 4126 women were categorized into different groups. The prevalence of subclinical hypothyroidism significantly decreased with age and sampling time, from 21.09 to 11.91% and from 28.57 to 10.67%, respectively (P < 0.001, respectively). Mean serum TSH, FT3, and FT4 levels decreased significantly with age (P = 0.017, < 0.001, < 0.001, respectively). In the context of sampling time, TSH levels from early in the morning were significantly higher (P < 0.001), while FT4 and FT3 levels were similar in different groups (P = 0.258, 0.300, respectively).Conclusions: The prevalence of subclinical hypothyroidism significantly decreased with increasing age and sampling time, as did the serum TSH levels. Even though, the establishment of reference interval of TSH level based on age or sampling time was not recommended. Full consideration of age and sampling time should be carefully taken before initiation of treatment. [ABSTRACT FROM AUTHOR]

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Vancomycindosing error and inappropriate monitoring is a common problem in hospital daily practice. In King Abdulaziz Medical City (KAMC) in Jeddah, a high percentage of abnormal vancomycin trough levels is still detected despite using the recommended dose. Therefore, the current research objective is to study the major causes of vancomycin dosing errors. This retrospective, single-center, cross-sectional study was carried out at KAMC hospital in Jeddah from January 1st until December 31st 2019. All adult patients (≥15 years) who received vancomycin and had an initial abnormal trough level at the measured steady-state were included in this study. 472 patients have met the study inclusion criteria. The current study evaluated the factors that play a role in causing vancomycin trough level abnormalities such as sampling time, vancomycin dosing, and patient's pharmacokinetic and pharmacodynamic variations. In this study, we found that pharmacokinetic and pharmacodynamic variability was attributed to 65% of vancomycin's abnormal trough level. Also, the result showed a significantly increased odds of the low trough in the non-elderly group (OR 6, 95% CI 2.48 – 14.9, P < 0.001) and febrile neutropenic patients (OR 2.21, 95% CI 1.119 – 4.365, P < 0.05). However, the odds of high trough levels were significantly elevated among patients who have CrCl < 50 ml/min (OR 5, 95% CI 1.262–20.539, P < 0.05). In addition, the present investigation revealed that the occurrence of abnormal vancomycin levels was not affected by daily duty time or working days (p > 0.05). The current study indicated that vancomycin dosing errors were common in KAMC patients; thus, there is an unmet need to evaluate the causes of vancomycin abnormal trough level and optimize a strategy that would enhance the therapeutic effectiveness and minimize the potential toxicity. [ABSTRACT FROM AUTHOR]

• Successful differentiation of PGI Hongyuan yak milk by using stable isotopes. • δ 13C and δ 18O was the main indication to identify the PGI Hongyuan yak milk. • Sampling dates affect stable isotope ratios of PGI Hongyuan yak milk. • Sampling locations do not affect stable isotope ratios of PGI Hongyuan yak milk. Hongyuan yak milk is a protected geographical indication (PGI) product of rich nutritional value, which is popular among consumers. Stable isotope ratio analysis (SIRA) is an effective way to protect the authenticity of the geographical origin of PGI products, and it is crucial to study the factors affecting stable isotopes. Firstly, we proved that the SIRA could be used to identify the geographical origin of Hongyuan yak milk, and that the identification accuracy in combination with δ 13C and δ 18O was 100 %. Secondly, we analyzed the effect of sampling selection on the stable isotopes of Hongyuan yak milk in practical applications, which showed that sampling time influenced the δ 13C, δ 2H, and δ 18O, while the sampling locations did not. There were interactions between the effect of sampling time and location on δ 2H and δ 18O. These results provide a reliable method for identifying PGI products and also provide new guidance on sampling models. [ABSTRACT FROM AUTHOR]

Improving production efficiency while enhancing pork quality is pivotal for strengthening sustainable pork production. Being able to study both gene expression and indices of pork quality from the same anatomical location of an individual animal would better enable research conducted to study relationships between animal growth and carcass merit. To facilitate gene expression studies, adipose and muscle tissue samples are often collected immediately following exsanguination to maximize RNA integrity, which is a primary determinant of the sensitivity of RNA-based assays, such as real-time PCR. However, collecting soft tissue samples requires cutting through the hide or skin. This leaves the underlying tissue exposed during scalding, poses possible food safety issues, and potentially confounds pork quality measures. To overcome these limitations, the effect of tissue sample timing post-harvest on RNA integrity, real-time PCR results, and pork quality measurements was investigated by sampling subcutaneous adipose tissue and longissimus thoracis et lumborum muscle immediately following either exsanguination, scalding, or chilling. Sampling time did not affect RNA quality, as determined by the RNA integrity number of RNA samples purified from either adipose (RIN; p > 0.54) or muscle tissue (p > 0.43). Likewise, the sampling time did not influence the results of real-time PCR analysis of gene expression when comparing RNA samples prepared from adipose or muscle tissue immediately following either exsanguination or scalding (p > 0.92). However, sampling tissue prior to scalding resulted in a greater visual color score (p < 0.001) and lesser L* (p < 0.001) and b* (p < 0.001) values without impacting the 24 h pH (p < 0.41). These results suggested that if both RNA-based assays and meat quality endpoints are to be performed at the same anatomical location on an animal, tissue sampling to facilitate RNA-based assays should occur at a time point immediately following scalding. These findings demonstrated that sampling of adipose and muscle tissue can be delayed until after scalding/dehairing without decreasing the RNA integrity or altering the results of real-time PCR assays, while doing so was associated with little impact on measures of pork quality.