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18 results on '"Sanchez-Alonso, Patricia"'

5. A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer

Author

Sanchez-Alonso, Patricia, primary, Cobos-Justo, Elena, additional, Avalos-Rangel, Miguel Angel, additional, López-Reyes, Lucía, additional, Paniagua-Contreras, Gloria Luz, additional, Vaca-Paniagua, Felipe, additional, Anastacio-Marcelino, Estela, additional, López-Ochoa, Ana Jaqueline, additional, Pérez Marquez, Victor M., additional, Negrete-Abascal, Erasmo, additional, and Vázquez-Cruz, Candelario, additional

Published
2023
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8. A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin

Author

Chantes-Guerra, Alberto, primary, Maldonado-Puga, Samantha, additional, Rojas-Ruiz, Norma, additional, Rea-Hernandez, Ismael, additional, Montes-Garcia, Fernando J., additional, Trujillo-Ruiz, Hector, additional, Yañez-Aguilar, Ivan E., additional, Vazquez-Cruz, Candelario, additional, Sanchez-Alonso, Patricia, additional, and Negrete-Abascal, Erasmo, additional

Published
2022
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11. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis

Author

Kamper, Jorg, Kahmann, Regine, Bolker, Michael, Ma, Li-Jun, Brefort, Thomas, Saville, Barry J., Banuett, Flora, Kronstad, James W., Gold, Scott E., Muller, Olaf, Perlin, Michael H., Wosten, Han A. B., de Vries, Ronald, Ruiz-Herrera, Jose, Reynaga-Pena, Cristina G., Snetselaar, Karen, McCann, Michael, Perez-Martin, Jose, Feldbrugge, Michael, Basse, Christoph W., Steinberg, Gero, Ibeas, Jose I., Holloman, William, Guzman, Plinio, Farman, Mark, Stajich, Jason E., Sentandreu, Rafael, Gonzalez-Prieto, Juan M., Kennell, John C., Molina, Lazaro, Schirawski, Jan, Mendoza-Mendoza, Artemio, Greilinger, Doris, Munch, Karin, Rossel, Nicole, Scherer, Mario, Vraneš, Miroslav, Ladendorf, Oliver, Vincon, Volker, Fuchs, Uta, Sandrock, Bjorn, Meng, Shaowu, Ho, Eric C. H., Cahill, Matt J., Boyce, Kylie J., Klose, Jana, Klosterman, Steven J., Deelstra, Heine J., Ortiz-Castellanos, Lucila, Li, Weixi, Sanchez-Alonso, Patricia, Schreier, Peter H., Hauser-Hahn, Isolde, Vaupel, Martin, Koopmann, Edda, Friedrich, Gabi, Voss, Hartmut, Schluter, Thomas, Margolis, Jonathan, Platt, Darren, Swimmer, Candace, Gnirke, Andreas, Chen, Feng, Vysotskaia, Valentina, Mannhaupt, Gertrud, Guldener, Ulrich, Munsterkotter, Martin, Haase, Dirk, Oesterheld, Matthias, Mewes, Hans-Werner, Mauceli, Evan W., DeCaprio, David, Wade, Claire M., Butler, Jonathan, Young, Sarah, Jaffe, David B., Calvo, Sarah, Nusbaum, Chad, Galagan, James, and Birren, Bruce W.

Abstract

Author(s): Jörg Kämper (corresponding author) [1]; Regine Kahmann (corresponding author) [1]; Michael Bölker (corresponding author) [2]; Li-Jun Ma [3]; Thomas Brefort [1]; Barry J. Saville [4, 27]; Flora Banuett [5]; [...]

Published
2006
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12. Organization of chromosome ends in Ustilago maydis. RecQ-like helicase motifs at telomeric regions

Author

Sanchez-Alonso, Patricia and Guzman, Plinio

Subjects
Telomeres -- Research, Smut fungi -- Genetic aspects, DNA sequencers -- Research, Biological sciences
Abstract

In this study we have established the structure of chromosome ends in the basidiomycete fungus Ustilago maydis. We isolated and characterized several clones containing telomeric regions and found that as in other organisms, they consist of middle repeated DNA sequences. Two principal types of sequence were found: UTASa was highly conserved in nucleotide sequence and located almost exclusively at the chromosome ends, and UTASb was less conserved in nucleotide sequence than UTASa and found not just at the ends but highly interspersed throughout the genome. Sequence analysis revealed that UTASa encodes an open reading frame containing helicase motifs with the strongest homology to RecQ helicases; these are DNA helicases whose function involves the maintenance of genome stability in Saccharomyces cerevisiae and in humans, and the suppression of illegitimate recombination in Escherichia coli. Both UTASa and UTASb contain a common region of about 300 bp located immediately adjacent to the telomere repeats that are also found interspersed in the genome. The analysis of the chromosome ends of U. maydis provides information on the general structure of chromosome ends in eukaryotes, and the putative RecQ helicase at UTASa may reveal a novel mechanism for the maintenance of chromosome stability.

Published
1998

13. Detection of genetic variation in Ustilago maydis strains by probes derived from telomeric sequences

Author

Sanchez-Alonso, Patricia, Valverde, Maria Elena, Paredes-Lopez, Octavio, and Guzman, Plinio

Subjects
Yeast fungi -- Genetic aspects, DNA -- Analysis, Telomeres -- Analysis, Biological sciences
Abstract

Genetic variation in basiodiomycetous fungus Ustilago maydis was investigated by fingerprint analysis with probes containing telomeric DNA. Group differences were observed between the endmost restriction fragments (EFs) and the endmost-associated restriction fragments (EAFs) of the chromosomes within strains. EFs showed similar lengths in two strains but were of different lengths in the other strains. Analysis of the EAFs showed the presence of distinct bands in the terminal and interstitial sites of the chromosomes in all strains.

Published
1996

16. The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis

Author

Bautista-Espana, Dolores, Anastacio-Marcelino, Estela, Horta-Valerdi, Guillermo, Celestino-Montes, Antonio, Kojić, Milorad, Negrete-Abascal, Erasmo, Reyes-Cervantes, Hortensia, Vazquez-Cruz, Candelario, Guzman, Plinio, Sanchez-Alonso, Patricia, Bautista-Espana, Dolores, Anastacio-Marcelino, Estela, Horta-Valerdi, Guillermo, Celestino-Montes, Antonio, Kojić, Milorad, Negrete-Abascal, Erasmo, Reyes-Cervantes, Hortensia, Vazquez-Cruz, Candelario, Guzman, Plinio, and Sanchez-Alonso, Patricia

Abstract

In this study, we investigated the reverse transcriptase subunit of telomerase in the dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all of the characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, and reduced survival, that were reminiscent of the traits observed in est2 budding yeast mutants. Telomerase activity was observed in wild-type fungus sporidia but not those of the disruption mutant. The introduction of a self-replicating plasmid expressing Trt1 into the mutant strain restored growth proficiency and replicative potential. Analyses of trt1 crosses in planta suggested that Trt1 is necessary for teliospore formation in homozygous disrupted diploids and that telomerase is haploinsufficient in heterozygous diploids. Additionally, terminal restriction fragment analysis in the progeny hinted at alternative survival mechanisms similar to those of budding yeast.

Published
2014

18. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis

Author

National Human Genome Research Institute (US), Bayer, National Institutes of Health (US), Federal Ministry of Education and Research (Germany), Max Planck Society, Natural Sciences and Engineering Research Council of Canada, Canada Foundation for Innovation, Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Alexander von Humboldt Foundation, European Commission, Kämper, Jörg, Kahmann, Regine, Bölker, Michael, Ma, Li-Jun, Brefort, Thomas, Saville, Barry J., Banuett, Flora, Kronstad, James W., Gold, Scott E., Müller, Olaf, Perlin, Michael H., Wösten, Han A. B., Vries, Ronald de, Ruiz-Herrera, José, Reynaga-Peña, Cristina G., Snetselaar, Karen, McCann, Michael, Pérez-Martín, José, Feldbrügge, Michael, Basse, Christoph W., Steinberg, Gero, Ibeas, Jose I., Holloman, William K., Guzman, Plinio, Farman, Mark, Stajich, Jason E., Sentandreu, Rafael, González-Prieto, Juan M., Kennell, John C., Molina Delgado, Lázaro, Schirawski, Jan, Mendoza-Mendoza, Artemio, Greilinger, Doris, Münch, Karin, Rössel, Nicole, Scherer, Mario, Vraneš, Miroslav, Ladendorf, Oliver, Vincon, Volker, Fuchs, Uta, Sandrock, Björn, Meng, Shaowu, Ho, Eric C. H., Cahill, Matt J., Boyce, Kylie J., Klose, Jana, Klosterman, Steven J., Deelstra, Heine J., Ortiz-Castellanos, Lucila, Li, Weixi, Sanchez-Alonso, Patricia, Schreier, Peter H., Häuser-Hahn, Isolde, Vaupel, Martin, Koopmann, Edda, Friedrich, Gabi, Voss, Hartmut, Schlüter, Thomas, Margolis, Jonathan, Platt, Darren, Swimmer, Candace, Gnirke, Andreas, Chen, Feng, Vysotskaia, Valentina, Mannhaupt, Gertrud, Güldener, Ulrich, Münsterkötter, Martin, Haase, Dirk, Oesterheld, Matthias, Mewes, Hans-Werner, Mauceli, Evan W., DeCaprio, David, Wade, Claire M., Butler, Jonathan, Young, Sarah, Jaffe, David B., Calvo, Sarah, Nusbaum, Chad, Galagan, James, Birren, Bruce W., National Human Genome Research Institute (US), Bayer, National Institutes of Health (US), Federal Ministry of Education and Research (Germany), Max Planck Society, Natural Sciences and Engineering Research Council of Canada, Canada Foundation for Innovation, Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Alexander von Humboldt Foundation, European Commission, Kämper, Jörg, Kahmann, Regine, Bölker, Michael, Ma, Li-Jun, Brefort, Thomas, Saville, Barry J., Banuett, Flora, Kronstad, James W., Gold, Scott E., Müller, Olaf, Perlin, Michael H., Wösten, Han A. B., Vries, Ronald de, Ruiz-Herrera, José, Reynaga-Peña, Cristina G., Snetselaar, Karen, McCann, Michael, Pérez-Martín, José, Feldbrügge, Michael, Basse, Christoph W., Steinberg, Gero, Ibeas, Jose I., Holloman, William K., Guzman, Plinio, Farman, Mark, Stajich, Jason E., Sentandreu, Rafael, González-Prieto, Juan M., Kennell, John C., Molina Delgado, Lázaro, Schirawski, Jan, Mendoza-Mendoza, Artemio, Greilinger, Doris, Münch, Karin, Rössel, Nicole, Scherer, Mario, Vraneš, Miroslav, Ladendorf, Oliver, Vincon, Volker, Fuchs, Uta, Sandrock, Björn, Meng, Shaowu, Ho, Eric C. H., Cahill, Matt J., Boyce, Kylie J., Klose, Jana, Klosterman, Steven J., Deelstra, Heine J., Ortiz-Castellanos, Lucila, Li, Weixi, Sanchez-Alonso, Patricia, Schreier, Peter H., Häuser-Hahn, Isolde, Vaupel, Martin, Koopmann, Edda, Friedrich, Gabi, Voss, Hartmut, Schlüter, Thomas, Margolis, Jonathan, Platt, Darren, Swimmer, Candace, Gnirke, Andreas, Chen, Feng, Vysotskaia, Valentina, Mannhaupt, Gertrud, Güldener, Ulrich, Münsterkötter, Martin, Haase, Dirk, Oesterheld, Matthias, Mewes, Hans-Werner, Mauceli, Evan W., DeCaprio, David, Wade, Claire M., Butler, Jonathan, Young, Sarah, Jaffe, David B., Calvo, Sarah, Nusbaum, Chad, Galagan, James, and Birren, Bruce W.

Abstract

Ustilago maydis is a ubiquitous pathogen of maize and a well-established model organism for the study of plant-microbe interactions. This basidiomycete fungus does not use aggressive virulence strategies to kill its host. U. maydis belongs to the group of biotrophic parasites (the smuts) that depend on living tissue for proliferation and development. Here we report the genome sequence for a member of this economically important group of biotrophic fungi. The 20.5-million-base U. maydis genome assembly contains 6,902 predicted protein-encoding genes and lacks pathogenicity signatures found in the genomes of aggressive pathogenic fungi, for example a battery of cell-wall-degrading enzymes. However, we detected unexpected genomic features responsible for the pathogenicity of this organism. Specifically, we found 12 clusters of genes encoding small secreted proteins with unknown function. A significant fraction of these genes exists in small gene families. Expression analysis showed that most of the genes contained in these clusters are regulated together and induced in infected tissue. Deletion of individual clusters altered the virulence of U. maydis in five cases, ranging from a complete lack of symptoms to hypervirulence. Despite years of research into the mechanism of pathogenicity in U. maydis, no 'true' virulence factors had been previously identified. Thus, the discovery of the secreted protein gene clusters and the functional demonstration of their decisive role in the infection process illuminate previously unknown mechanisms of pathogenicity operating in biotrophic fungi. Genomic analysis is, similarly, likely to open up new avenues for the discovery of virulence determinants in other pathogens. ©2006 Nature Publishing Group.

Published
2006

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