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Introduction: Patient’s environment in Health care settings responsible for causing variety of infections to patients and to the healthcare professions. Many of them are MDRO which may get transmitted as hospitals acquired infections that increases the chances of mortality and morbidity. These study was undertaken to isolate and identify aerobic bacteria with their resistance pattern in high touch areas of critical and noncritical care units of tertiary care hospital in absence of defined outbreaks. Materials and Methods: The cross sectional analytical study was carried out in the Microbiology Department, after obtaining the Institutional Ethics Committee clearance with waiver of consent during a period of two months. A total 100 samples from nine surface locations of critical and non critical care units of tertiary care hospital were included in this study. All tubes were vortexed and 0.5 ml of the peptone water was inoculated immediately on Blood Agar and MacConkey Agar for semi-quantitative estimation of the organisms. Result: The environmental screening for MDROs from high touch areas was carried out in the 650 bedded tertiary care hospital.. A total of 34/126 (26.98%) samples from various areas had the growth of microorganisms where Staphylococcus aureus predominated. 24.60% showed S. aureus growth while 2.38% were E.coli. A total of 47.05% strains were isolated from the critical care areas all of which were S. aureus whereas 52.94% strains were from the non-critical areas. Conclusion: This study established to isolate and identify aerobic bacteria with their resistance pattern in high touch areas of critical and non critical care units of tertiary care hospital in absence of defined outbreak. These creates awareness regarding emerging of MDROs in hospital settings and also leads to improve in imperfect techniques of hand hygiene practices as well as inadequate surface disinfection practices especially in high touch areas. Keywords: MDROs, MRSA, Blood aga

Introduction : According to the CDC, one-to-three cases out of 100 surgeries get infected. There is considerable variation in each class according to the type of surgery being performed. The incidence of infection varies from surgeon to surgeon, from hospital to hospital, from one surgical procedure to another and, most importantly, from one patient to another. The aim of this study was to identify the most common microorganism documented in post-operative wound sepsis in surgery wards of local tertiary hospitals situated in Chhattisgarh. Methods: This Retrospective & Analytical study involved Prior Consent from Hospital Authorities / Medical Superintendents of the Randomly selected Private Tertiary care hospitals to see the records of the patients from Medical Records Department ( MRD) The study included 100 randomly selected case sheets / records of the patients suffering from SSIs in the various surgical wards in local tertiary care centres . Patients of both sex, age > 14 years, who had surgical wound pus discharge, with serous or seropurulent discharge and with signs of sepsis present concurrently were included. Results : Out of the 100 selected case sheets / records of patient’s samples processed, In 91% of records it was found that it yielded aerobic bacterial growth . Monomicrobial growth was seen in 92 samples while 8 samples showed polymicrobial growth. The mean age of the patients was 45.7 years (range 14 to 79 years) and the peak incidence of SSI was observed in age group > 50 years (55%). Among the 100 records / cases, bacterial isolates, S. aureus (52%) and E. coli (24.%) were the commonest organisms. Conclusion: . The information obtained from this study allows a better understanding of the microbial etiology of SSIs in local hospitals which may have epidemiological and therapeutic implications. Using the results of this study, an initiative for establishing improved hospital antimicrobial policy and antimicrobial prescribing guidelines can be undertaken.