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Asarum chungbuensis, a species endemic to Korea, has a limited distribution across the Korean Peninsula and is used in traditional medicine. Despite its importance, the genome structure, genetic composition, and phylogenetic relationships based on its chloroplast genome have not been documented. In this study, the complete chloroplast genome of A. chungbuensis was newly assembled. The chloroplast genome is 190,179 base pairs (bp) long, and the overall GC content (%) of the plastid was 36.8%. The chloroplast genome size of A. chungbuensis is longer than that of the normal chloroplast genome (160 kb) because of an inverted small single-copy (SSC) duplication that incorporates the SSC into an inverted repeat (IR) region. By extension, this duplication event causes this chloroplast genome to lack an SSC, unlike other formal structures. The chloroplast genome, with a tripartite structure, consisted of a single-copy region of 93,351 bp with a 34.6% GC content and two IR regions, each with a length of 48,414 bp and a 38.8% GC content. Additionally, it was found to have 113 genes, including 79 PCG genes, 30 tRNA genes, and four rRNA genes. Phylogenetic analysis revealed that A. chungbuensis was grouped with A. heterotropoides var. seoulense, which diverged from the clade comprising A. koreanum and A. patens. The newly sequenced A. chungbuensis chloroplast genome could provide valuable genomic information for determining unique genome structures, especially for the assessment of genetic diversity, phylogenetic relationships, species conservation, and biogeographic studies of the genus Asarum.

This study aims to report the complete chloroplast genome of Solidago altissima L., a globally recognized invasive plant. The complete genome length of S. altissima is 152,961 bp; S. altissima has a typical quadripartite structure (including a large single copy of 84,829 bp, a small single copy of 18,084 bp, and two inverted repeat regions of 25,024 bp), which is commonly found in angiosperms. The genome contains 129 genes, consisting of 85 coding sequences, 36 tRNA genes, and 8 rRNA genes. To understand the phylogenetic relationship between S. altissima and its related species, maximum likelihood analysis was performed. The results revealed that S. altissima is closely related to Symphyotrichum subulatum. The findings of the present study could provide fundamental data for the future phylogenetic and evolutionary studies, while also research on species invasion and resolving complexity of S. altissima.

AbstractWe sequenced and published the chloroplast genome of Commelina caroliniana Walter, which was previously misidentified as C. diffusa owing to their morphological similarities until 1989. The genome of C. caroliniana is 160,857 bp long [large single copy region: 88,064 bp; a small single copy region: 18,549 bp; two inverted repeat regions: 27,122 bp] and has a GC content of 35.7%. The genome comprises 133 genes, including 87 coding sequences (CDSs), 38 tRNAs, and eight rRNAs. The phylogenetic relationship between C. caroliniana and related species was analyzed using the maximum likelihood method based on the 79 CDSs of the chloroplast genome. Phylogenetic analysis revealed that C. caroliniana is closely related to C. communis. Our findings will contribute to studies on species identification and phylogenetic and evolutionary research. These results enhance our understanding of the Commelina genus.

Adenostemma madurense D.C. belongs to the family Asteraceae and is a wild annual herb found in Korea. In this study, we determined the phylogenetic position of A. madurense by identifying its complete chloroplast sequence. The result indicated a genome size of 150,054 bp, which was composed of a large single copy of 82,008 bp, two inverted repeats of 24,952 bp each, and a small singlecopy of 18,142 bp. Overall, 128 unique genes were identified, including 85 protein-coding, 35 tRNA, and 8 rRNA genes. A comparison of the A. madurense and A. lavenia chloroplast genomes revealed seven variations. Phylogenetic analysis revealed that A. madurense formed a clade with A. lavenia and had a well-supported phylogenetic relationship with Asteraceae. The first complete chloroplast genome of A. madurense was obtained, which would be helpful in explaining the process of speciation in Adenostemma and the development of molecular markers.

We determined the complete chloroplast genome sequence of Epilobium parviflorum Schreb. The genome is 161,284 bp in length [including a large single copy (LSC): 89,063 bp; a small single copy (SSC): 17,323 bp; and two inverted repeat (IR) regions: 27,499 bp] with 38.1% GC content and contains 84 coding genes (CDS), 37 transfer RNA genes, and 8 rRNA genes. Among them, infA was annotated as a pseudogene. The maximum-likelihood analysis based on CDS sequences of chloroplast genomes was conducted for investigating the phylogenetic relationship among Epilobium and related species. The results showed a close relationship between E. parviflorum and E. hirsutum L. Our study added new genetic information of E. parviflorum and helped to understand the relationship with related species and evolutionary history in Onagraceae.

Micranthes octopetala (Nakai) Y.I.Kim & Y.D. Kim et al. 2015, which belongs to the family Saxifragaceae, is a perennial herb endemic to Korea. M. octopetala was originally treated as a synonym of M. manchuriensis. However, in 2015, molecular phylogenetic analysis confirmed that M. octopetala is an independent species. In this study, the plastid genome of M. octopetala was sequenced for the first time, and the taxonomic position of this species was identified. The complete plastid genome of M. octopetala has a total length of 149 751 bp (large single copy: 83 083 bp; small single copy: 17 196 bp; inverted repeat: 24 736 bp), containing 130 genes, including 79 CDS, 30 tRNAs, and 4 rRNAs. Moreover, the absence of intron in the rpl2 gene, which is a common feature of Saxifragaceae, was confirmed. Phylogenetic analysis based on 79 protein-coding genes from 21 species revealed that M. octopetala belongs to the genus Micranthes, being a sister to other Micranthes species. The plastid genome of M. octopetala obtained in this study provides fundamental information for future studies on the genus Micranthes.

Aconitum quelpaertense Nakai is an endemic species of Jeju Island of South Korea. Here, we characterized complete chloroplast genome sequence of A. quelpaertense and inferred its phylogenetic position within the genus Aconitum. The complete chloroplast genome was 155,636 bp in length with typical quadripartite structure. Length of large single copy was 86,695 bp, whereas small single copy was 17,087 bp. A pair of inverted repeats were 25,927 bp. In total, 128 genes were encoded and comprising 83 protein-coding genes, 37 tRNA genes and eight rRNA genes. rpl32 and rps16 genes were pseudogenized in this plastome. The phylogenetic analysis based on complete chloroplast genome sequences showed that A. quelpaertense was closely related to another endemic species of South Korea, A. pseudolaeve.

We determined the complete chloroplast genome sequence of Ligustrum foliosum Nakai, an endemic species of Korea. The plastome was 165,731 bp long, with a large single copy (90,009 bp), a small single copy (11,448 bp), and a pair of inverted repeats (32,137 bp). Moreover, 113 genes were encoded, including 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis of 18 species using concatenated 78 protein-coding genes (excluding one pseudogene) revealed that L. foliosum is most closely related to L. quihoui, having strong bootstrap values. This study is the first report of the chloroplast genome of L. foliosum and provides important information for future phylogenetic studies on Ligustrum and distinguishing closely related species of Ligustrum.

Summary: The circadian clock regulates temporal metabolic activities, but how it affects lipid metabolism is poorly understood. Here, we show that the central clock regulators LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) regulate the initial step of fatty acid (FA) biosynthesis in Arabidopsis. Triacylglycerol (TAG) accumulation in seeds was increased in LHY-overexpressing (LHY-OE) and decreased in lhycca1 plants. Metabolic tracking of lipids in developing seeds indicated that LHY enhanced FA synthesis. Transcript analysis revealed that the expression of genes involved in FA synthesis, including the one encoding β-ketoacyl-ACP synthase III (KASIII), was oppositely changed in developing seeds of LHY/CCA1-OEs and lhycca1. Chromatin immunoprecipitation, electrophoretic mobility shift, and transactivation assays indicated that LHY bound and activated the promoter of KASIII. Furthermore, phosphatidic acid, a metabolic precursor to TAG, inhibited LHY binding to KASIII promoter elements. Our data show a regulatory mechanism for plant lipid biosynthesis by the molecular clock.

Polygonatum infundiflorum Y.S. Kim, B.U. Oh & C.G. Jang et al. is a Korean endemic species. This is first report on the complete chloroplast genome sequence of P. infundiflorum. The complete chloroplast genome length was 154,578 bp with a GC content of 37.7%. The large single-copy (LSC) region was 83,527 bp long, and the small single-copy (SSC) region was 18,457 bp long. The paired inverted repeats (IRs) were 26,297 bp and separated the LCS and SSC regions. There were 113 genes, comprising 78 protein-coding genes, four rRNA genes, 30 tRNA genes, and one pseudogene (infA). In total, 16 genes contained one intron, and two genes contained two introns. Phylogenetic analysis suggested that Polygonatum was divided into three sections, each forming a monophyletic group. P. infundiflorum was sister to P. macropodum and formed a monophyletic group with P. inflatum. This study provides basic information for future research and contributes to taxonomic and genetic studies on Polygonatum.

Paraphlomis is a genus belonging to the family Lamiaceae with approximately 24 species distributed mainly in East Asia. Among these, the distribution of one species, Paraphlomis koreana S.C. Ko et G.Y. Chung, is restricted to South Korea. Here, we determined the complete chloroplast (cp) genome of P. koreana and analyzed its relationship with related species. The complete cp genome sequence of P. koreana is 152,128 bp in length and consists of large (83,251 bp) and small (17,631 bp) single-copy regions and a pair of identical inverted repeats (25,623 bp). It contains 87 protein-coding genes, 37 tRNA genes, four rRNA genes, and one pseudogene (infA). Phylogenetic analysis of 22-related taxa inferred from cp genomes revealed the position of P. koreana (endemic to the Korean Peninsula) and showed that this species is closely related to P. javanica. The complete cp genome sequence of P. koreana obtained in this study provides important information for phylogenetic and evolutionary studies of Lamiaceae.

Abstract This study was to understand the genetic structure and diversity of the Korean Malus species. We used genotyping-by-sequencing (GBS) technology to analyze samples of 112 individuals belonging to 18 populations of wild Malus spp. Using GBS, we identified thousands of single nucleotide polymorphisms in the species analyzed. M. baccata and M. toringo, two dominant mainland species of the Korean Peninsula, were distinguishable based on their genetic structure. However, M. toringo collected from Jeju Island exhibited a different genetic profile than that from the mainland. We identified M. cf. micromalus as a hybrid resulting from the Jeju Island M. toringo (pollen donor) and the mainland M. baccata, (pollen recipient). Putative M. mandshurica distributed on the Korean Peninsula showed a high structural and genetic similarity with M. baccata, indicating that it might be an ecotype. Overall, this study contributes to the understanding of the population history and genetic structure of Malus in the Korean Peninsula.

Objectives: Rapid and accurate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic tests are crucial for controlling the spread of infections in emergency settings. This study evaluated the diagnostic accuracy of a point-of-care (POC) test based on loop-mediated isothermal amplification (LAMP) that produces rapid results within 30 min. Methods: We prospectively included adult patients (age >19 years) who were diagnosed with SARS-CoV-2 infection within the last 3 days and symptomatic patients who had visited the emergency room. Posterior nasopharyngeal (PNP) swabs and throat swabs collected by physicians were used to test the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy, and Cohen's Kappa coefficient (k) of the POC index and reference reverse transcription quantitative polymerase chain reaction (RT-qPCR) test devices. Results: Of the 352 participants, 102 (29.0%) tested positive via the RT-PCR-based reference test device; the RT-LAMP-based POC test had a sensitivity of 70.6% and specificity of 98.0%, with 93.5% PPV, 89.1% NPV, 35.5% PLR, and 3.4% NLR. Cohen's k correlation of results from the two devices was 0.74. The cycle threshold value between the positive and negative POC test results differed (17.6 vs. 24.6, p

Lespedeza tricolor is a species found in the southern province of Korea, thought to be endemic to Korea. The complete plastid genome of this legume was sequenced in this study. DNA from L. tricolor was extracted, sequenced, and assembled into the complete plastid genome. We used 18 species of the family Fabaceae and 77 protein-coding genes to perform phylogenetic analysis. The plastid genome is 149,038 bp in length, with large (82,495 bp) and small (18,889 bp) single-copy regions, separated by a pair of inverted repeat regions (23,827 bp). It contains 83 protein-coding genes, eight rRNAs, 37 tRNAs, and two pseudogenes (rpl22 and infA). Our phylogenetic analysis suggests that the genus Lespedeza is monophyletic and L. tricolor is closely related to L. maritima and L. buergeri. In this study, we identified the phylogenetic position of L. tricolor and provided the data that can be used in various ways in future studies.

Chloroplast genomes are valuable for inferring evolutionary relationships. We report the complete chloroplast genomes of 36 Corydalis spp. and one Fumaria species. We compared these genomes with 22 other taxa and investigated the genome structure, gene content, and evolutionary dynamics of the chloroplast genomes of 58 species, explored the structure, size, repeat sequences, and divergent hotspots of these genomes, conducted phylogenetic analysis, and identified nine types of chloroplast genome structures among Corydalis spp. The ndh gene family suffered inversion and rearrangement or was lost or pseudogenized throughout the chloroplast genomes of various Corydalis species. Analysis of five protein-coding genes revealed simple sequence repeats and repetitive sequences that can be potential molecular markers for species identification. Phylogenetic analysis revealed three subgenera in Corydalis. Subgenera Cremnocapnos and Sophorocapnos represented the Type 2 and 3 genome structures, respectively. Subgenus Corydalis included all types except type 3, suggesting that chloroplast genome structural diversity increased during its differentiation. Despite the explosive diversification of this subgenus, most endemic species collected from the Korean Peninsula shared only one type of genome structure, suggesting recent divergence. These findings will greatly improve our understanding of the chloroplast genome of Corydalis and may help develop effective molecular markers.

Abstract Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2, PDAT1, and LEC1, was increased in developing seeds of FAX1- and/or ABCA9-OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

Syneilesis aconitifolia is an herbaceous perennial of the Asteraceae family native to forests in China, Korea, Japan, and eastern Russia. In Korea, the young leaves of the plant are edible and the extract is known to have antitumor effects. The length of the complete plastome was found to be 150,773 bp, including 130 genes, consisting of 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The assembled plastome showed typical structure and gene content of the angiosperm plastome, which includes two inverted repeats (IR) regions of 24839 bp, a large single copy (LSC) region of 82911 bp, and a small single-copy (SSC) region of 18184 bp. The total G/C content in the S. aconitifolia plastome was 37.5%. The maximum likelihood (ML) phylogenetic tree strongly supports that S. aconitifolia is closely related to the hosts of Ligularia fischeri. This study reports the first complete chloroplast genome of the genus Syneilesis and will contribute to the phylogenetics of the family Asteraceae.

Stress conditions can induce translocation of glyceraldehyde-3-phosphate dehydrogenase (GAPC) to the nucleus. Here Kim et al. show that in Arabidopsis, GAPC can interact with the NF-YC transcription factor subunit, enhance expression of heat-inducible genes and promote heat tolerance.

Primula comprises many species of horticultural value. In Korea, six species grow in the wild. Yamazaki classified the variety Primula modesta var. fauriei into P. modesta var. hannasanensis and P. modesta var. koreana based on the differences in leaf morphology. We compared the chloroplast genome sequences of the two Korean endemic Primula varieties and found that both had the typical quadripartite structure of angiosperms. The chloroplast genome size of P. modesta var. hannasanensis is 154,772 bp, including an 85,238 bp large single-copy region and a 17,790 bp small single-copy region, whereas that of P. modesta var. koreana is 154,667 bp, including an 85,152 bp large single-copy region and a 17,771 bp small single-copy region. The inverted repeat region is 25,872 bp in both varieties. We predicted 129 genes—84 protein-coding genes, 8 rRNAs, and 37 tRNAs. We identified 536 single-nucleotide polymorphisms and 501 indels between the varieties. Phylogenetic analysis revealed that the two varieties formed a sister group in the clade P. knuthiana–P. stenocalyx. This study will contribute to phylogenetic, taxonomic, and evolutionary studies of the genus Primula; it will also contribute to the analysis of the genetic diversity of the two varieties, and to the development of identification markers.

Fly ash, the main component for controlled low-strength material (CLSM), has physical and chemical characteristics according to the resources used in the thermal power plant, and thus fly ash type can influence the physical and strength properties of CLSM. This study investigates the effect of fly ash type on the engineering properties of CLSM and establishes relationships between mechanical properties of CLSM and shear wave velocity (SWV) for long curing times. Six fly ashes with different physical properties and chemical components are used for preparing the CLSM mixtures. The air content, unit weight, flowability, and setting time of CLSM are measured. Unconfined compressive strength (UCS) and elastic modulus (E) are obtained from unconfined compressive tests, and SWV (Vs) is determined using a bender element-based wave measurement system. Experimental results show that the stiffness and strength characteristics of CLSM are relevant to the contents of two oxides (SiO2 and Al2O3) and the fineness of fly ash. Because the evolution of SWV is influenced by the fly ash type, the relationships UCS-Vs and E-Vs are well established. Thus, considering the fly ash type, shear wave monitoring may be effectively used for estimating strength and stiffness characteristics of CLSM.

The complete plastid genome of Chamaecyparis obtusa was sequenced and analyzed in this study. It was found to be 126,821 bp long. The guanine-cytosine content of the whole genome was 35.1%, and there were 83 unique protein-coding genes, 33 tRNAs, and 4 rRNAs. Furthermore, an overlap of 120 bp was found (including trnQ-UUG). The order and organization of these genes were consistent with those of other complete plastid genomes from the Cupressaceae. A phylogenetic tree was constructed according to the 83 protein-coding genes found, which demonstrated that C. obusa had sister relationships within the genus Chamaecyparis.

In the present study, we analyzed the complete chloroplast genome sequence of Betula costata using the Ion Torrent platform. The chloroplast genome of B. costata was found to be 160,547 bp in length, with a large single-copy (LSC) region of 89,385 bp, a small single-copy (SSC) region of 19,038 bp, and a pair of inverted repeats (IRs) of 26,062 bp each. The overall GC content of the chloroplast genome was 36.1%. It contained 132 genes, including 87 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The phylogenetic analysis indicated that B. costata is closely related to Betula nana and Betula pubescens.

The completed chloroplast genome of Gastrodia elata Blume (G. elata) from Korea was determined in this study. The cpDNA is 35,230 bp in length and lacked the large and small single copy (LSC and SSC) regions, due to the lost inverted repeat (IR). The overall AT content is 73.30%, and the cpDNA contains 20 protein-coding genes, 5 tRNA genes, and 3 rRNA genes. Remarkably, the Korean G. elata cp genome was 74 bp smaller than that of the Chinese G. elata. It revealed substantial sequence variants 495 SNPs and 75 InDels between the two G. elata genomes.

Intercontinental floristic disjunction between East Asia and North America in the Northern Hemisphere has received much attention during the past decades, but few studies have focused on the family level. Melanthiaceae, containing 196 species and 17 genera circumscribed in five tribes, is disjunctly distributed in Eurasia and North America. It is one of the foremost models for studying the evolution of biogeographic patterns in this region. Here, we present a fossil-calibrated, molecular phylogeny of Melanthiaceae based on two chloroplast DNA datasets: one dataset includes extensive sampling (94 species representing all 17 genera of Melanthiaceae) of four chloroplast DNA regions (atpB, rbcL, matK, and ndhF) and the other includes six species representing all tribes of the family for 78 coding genes of the chloroplast genome. Within this framework, we infer the historical biogeography of Melanthiaceae. Both datasets produce well-resolved phylogenies of Melanthiaceae showing the monophyly of the family and the relationships among the five tribes. Melanthieae is found to be sister to the rest of the tribes of the family and the remaining taxa are divided into two major clades consisting of the Chionographideae + Heloniadeae clade and the Parideae + Xerophylleae clade. The molecular dating and the ancestral area analyses suggest that Melanthiaceae most likely originated in North America with its crown group dated at 92.1 mya in the late Cretaceous. The favored ancestral areas at the crown lineages of tribes are also in North America. In the family, seven independent migrations into East Asia from North America are inferred to have occurred in the Oligocene and the Miocene-Pliocene via historical paleo-land bridge connections. Cooling trends during the Oligocene resulted in the present East Asia-North America disjunct distribution, while the warm period during the middle Miocene and habitat heterogeneity stimulated diversification in East Asia. Our study provides the phylogenetic and biogeographical history of the Melanthiaceae and adds an example of “out of North America” migration in the biogeographic history of the Northern Hemisphere.

In the present study, chloroplast genome sequences of four species of Symplocos (S. chinensis for. pilosa, S. prunifolia, S. coreana, and S. tanakana) from South Korea were obtained by Ion Torrent sequencing and compared with the sequences of three previously reported Symplocos chloroplast genomes from different species. The length of the Symplocos chloroplast genome ranged from 156,961 to 157,365 bp. Overall, 132 genes including 87 functional genes, 37 tRNA genes, and eight rRNA genes were identified in all Symplocos chloroplast genomes. The gene order and contents were highly similar across the seven species. The coding regions were more conserved than the non-coding regions, and the large single-copy and small single-copy regions were less conserved than the inverted repeat regions. We identified five new hotspot regions (rbcL, ycf4, psaJ, rpl22, and ycf1) that can be used as barcodes or species-specific Symplocos molecular markers. These four novel chloroplast genomes provide basic information on the plastid genome of Symplocos and enable better taxonomic characterization of this genus.

The complete plastid genome of Corylopsis spicata was sequenced and analyzed in this study. It was found to be 159,507 bp and consisted of a large (88,243 bp) and small (18,716 bp) single-copy regions, separated by a pair of identical inverted repeats (26,274 bp). The GC content of the whole genome was 36.9%, and there were 85 unique protein-coding genes, 37 tRNA, and eight rRNA. The gene order and organization were consistent with those of other complete plastid genomes from the Hamamelidaceae. A phylogenetic tree was constructed based on 76 protein-coding genes that demonstrated a sister relationship within the genus Corylopsis.

The aim of the present study was to sequence and analyze the complete plastid genome (i.e. plastome) of Acer tegmentosum Maxim. The plastome of A. tegmentosum was 156,435 bp in length and included both large (86,265 bp) and small (18,102 bp) single-copy regions, which were separated by a pair of identical inverted repeats (26,034 bp). The plastome contained 77 unique protein-coding genes, 30 tRNA genes, and four rRNA genes. In addition, the gene order and organization of the A. tegmentosum plastome were consistent with those of plastomes from other members of the Sapindaceae, and the overall GC content of the plastome was 37.8%. A phylogenetic tree that was based on 76 protein-coding genes demonstrated a sister relationship within genus Acer.

Torreya nucifera (L.) Siebold & Zucc. (Taxaceae) is a tertiary relict tree species with a distribution that is limited to South Korea and Japan. In the present study, the complete chloroplast (cp) genome of T. nucifera was sequenced and analyzed. The genome was 136,985 bp in length and contained 118 genes, including 82 protein-coding genes, 33 tRNA genes, and 4 rRNA genes. Fifteen of the genes contained a single intron, whereas ycf3 contained two introns and there were no inverted repeat sequences in the genome. Phylogenetic analysis supported the monophyly of Torreya species within the Taxaceae and T. nucifera was closely related to its congener T. grandis.

The complete plastid genome of Sorbaria sorbifolia var. stellipila was sequenced and analyzed in this study. It was found to be 160,820 bp and consisted of a large (88,869 bp) and small (20,853 bp) single-copy regions, separated by a pair of identical inverted repeats (25,549 bp). The GC content of the whole genome was 37.8%, and there were 84 unique protein-coding genes, 37 tRNAs, and 8 rRNAs. The gene order and organization were consistent with those of other complete plastid genomes from the Rosaceae. The phylogenetic tree construct was based on 76 protein-coding genes and demonstrated a sister relationship with the Rosaceae.

Carpinus laxiflora is a member of the family Betulaceae. In the present study, we analyzed the complete chloroplast genome sequence of C. laxiflora using the Ion Torrent Platform. We found that the chloroplast genome of C. laxiflora was 159,255 bp in length with a large single copy (LSC) region of 88,359 bp, a small single copy (SSC) region of 18,764 bp, and a pair of inverted repeats (IRs) of 26,066 bp. The overall GC content of the chloroplast genome was 36.5%. It contained 135 genes, including 87 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. A phylogenetic analysis indicated that C. laxiflora is closely related to C. monbeigiana.

The complete genome of Vaccinium oldhamii plastid was sequenced and analyzed in this study. The V. oldhamii plastid a circular genome of 173,245 bp with 33 genes, including 88 protein, 37 tRNA, and eight rRNA genes. Phylogenetic analysis showed that species of Vaccinium were the first branching taxon In Ericaceae.

The complete chloroplast (cp) genome sequence of Actinidia rufa was determined by next-generation sequencing in this study. The whole cp genome was 156,543 bp in length, containing a large single-copy (LSC) of 88,435 bp and a small single-copy (SSC) region of 20,307 bp, which was separated by a pair of 23,900 bp inverted repeat (IR) regions. The genome contained 131 genes, including 84 protein-coding genes, 39 tRNA genes, 8 ribosomal RNA genes. Two events were found in the Actinidiaceae chloroplast genome. One was the deletion of the clpP gene and the other was the trnfM-CAU gene in the same direction in LSC region. The phylogenetic position of A. rufa was closely clustered with A. chinensis, A. deliciosa as sister species.

The complete chloroplast (cp) genome sequence of Larix kaempferi and L. olgensis var. koreana were determined by Ion torrent Platform sequencing in this study. The L. kaempferi cp genome was 122,158bp consists of two inverted repeat (IR) regions of 436bp each, a large single-copy (LSC) region of 65,394bp, and a small single-copy (SSC) region of 55,892bp. The chloroplast genome sequence of L. olgensis var. koreana was 122,573bp in length, consisting of two IRs (436bp), one LSC (65,597bp), and one SSC (56,104bp), and is longer than that of L. kaempferi. The genome contained 110 genes, including 71 protein-coding genes, 35 tRNA genes, and 4 ribosomal RNA genes. The 13 genes contain introns, including 12 genes with a single intron each and ycf3 gene with two introns. And, the rps12 gene is a trans-spliced gene. The phylogenetic analysis revealed that all sampled species in Pinaceae formed a monophyletic clade with high bootstrap value. The genus Larix is closely related to Pseudotsuga.

Fraxinus chiisanensis is endemic to Korea and restricted to local habitats with an extremely limited population size. The chloroplast genomic information can be used to formulate a comprehensive conservation strategy. In this study, the complete chloroplast (cp) genome sequence of F. chiisanensis was determined using next-generation sequencing. The entire cp genome was determined to be 155,571 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 86,412 of 17,767 bp, respectively, which were separated by a pair of 25,696 bp inverted repeat (IR) regions. The genome contained 132 genes, including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Among the 132 genes, seven coding genes, seven tRNA genes, and four rRNA genes occur in the two IR regions. The phylogenetic position of F. chiisanensis in Oleaceae was closely clustered with Olea, Chionanthus retusus, and Hesperelaea palmeri as sister species.

The purpose of this paper is to present a computerized integrated project management system and report results of a survey on the effectiveness of the system. The system consists of a scheduling system, material management system, labor/equipment system, and safety/quality control system. The backbone system is a scheduling system that adopts a production planning system and a project scheduling system. The lowest level in the scheduling system is a daily work management system, which is linked to each functional management system (i.e. material management system, labor/equipment system, and safety/quality control system). The paper focuses on the material management and scheduling systems to implement a material “pull” system to reduce material inventories on site. Details of material management and scheduling systems are discussed, and a sample application is presented to demonstrate the features of the proposed computer application system. The paper presents practitioners and researchers with a practical tool to integrate material management and scheduling systems for site personnel.

Information on the capacity of available workforce is important in production planning because a production unit's quantitative and qualitative capacities need to match its assigned loads. In the planning process, assigned loads and quantitative capacity are well defined quantitatively through quantity takeoff and database estimation. However, tracking and managing such workforce information, including skills, accident history and experiences, is a challenging task. This research seeks to develop a prototype workforce information database system that defines not only quantitative data but also qualitative measures in relation to load and capacity. A workforce information database system is described for use in workforce-level production planning. This database system tracks the daily quantitative and qualitative production capacities of each worker. The paper also explored the benefits and its applicability in production planning through a survey.