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Abstract Updates of SARS-CoV-2 vaccines are required to generate immunity in the population against constantly evolving SARS-CoV-2 variants of concerns (VOCs). Here we describe three novel in-silico designed spike-based antigens capable of inducing neutralising antibodies across a spectrum of SARS-CoV-2 VOCs. Three sets of antigens utilising pre-Delta (T2_32), and post-Gamma sequence data (T2_35 and T2_36) were designed. T2_32 elicited superior neutralising responses against VOCs compared to the Wuhan-1 spike antigen in DNA prime-boost immunisation regime in guinea pigs. Heterologous boosting with the attenuated poxvirus - Modified vaccinia Ankara expressing T2_32 induced broader neutralising immune responses in all primed animals. T2_32, T2_35 and T2_36 elicited broader neutralising capacity compared to the Omicron BA.1 spike antigen administered by mRNA immunisation in mice. These findings demonstrate the utility of structure-informed computationally derived modifications of spike-based antigens for inducing broad immune responses covering more than 2 years of evolved SARS-CoV-2 variants.

Outdoor physical activities and sports are growing in popularity: number of practitioners and professionals are increasing and number of active sport tourism businesses has multiplied. All these activities have become officialised, have grown into an emerging industry and play a powerful role as an economic driver. Qualified instructors are needed, as well as quality training for these professionals. This study focuses on climbing, mountaineering and winter sports instructor qualifications. Drawing on Bernstein's framework of the pedagogic device and with a particular focus on marketisation of education and professionalisation of sport, the purpose of this research is to explore the following questions: (a) How do stakeholders perceive the current mountaineering, climbing and winter sports curriculum and what are the main lines of improvement of the curriculum? (b) Does the curriculum meet the knowledge required by instructors in their occupations? And (c) What is the logic and awareness underlying the construction of the curriculum? Following a social-constructivist paradigm, in-depth semi-structured interviews and focus groups were carried out, with a sample of 46 key involved stakeholders. The data coding approach and theme development were done both inductively and deductively and with a latent and semantic focus, following thematic analysis. Participants specify clear lines of improvement of qualifications: stakeholders involved in curricular updating, the general structure of qualifications, admission tests, assessment, internships, model of the teacher and also highlighted some training gaps to be resolved due to qualifications breakdown. It is noted that curriculum updating follows a 'top-down' policy demand, that there is a marketisation of the qualifications that is evident in admission tests, assessment and internships. This study provides a critical and in-depth analysis based on the views of a variety of stakeholders that should be taken into consideration for the improvement of the curriculum of climbing, mountaineering and winter sports instructors.

The inadequate disposal of tires poses a significant threat to human health and requires effective recycling solutions. The crosslinked structure of rubber, formed through sulfur bridges during vulcanization, presents a major challenge for recycling because it prevents the rubber scraps from being reshaped thermoplastically. Reclaiming or devulcanization aims to reverse this crosslinking, allowing waste rubber to be transformed into products that can be reprocessed and revulcanized, thereby saving costs and preserving resources. Microwave technology shows promise for devulcanization due to its ability to break sulfur crosslinks. In this study, we investigate the devulcanization of ground tire rubber (GTR) through a combined process applied to samples from both car and truck tires subjected to varying periods of microwave irradiation (0, 3, 5 and 10 min). The devulcanized GTR was then blended with natural rubber (NR) and underwent a new vulcanization process, simulating recycling for novel applications. The GTR was mixed with NR in proportions of 0, 10, 30 and 50 parts per hundred rubber (phr). This study also examines the differences between the GTR from car tires and GTR from truck tires. The results showed that the treatment effectively breaks the crosslinks in the GTR, creating double bonds (C=C) and improving the mechanical properties of the revulcanized samples. The crosslinking density and related properties of the samples increased with treatment time, reaching a maximum at 5 min of microwave treatment, followed by a decrease at 10 min. Additionally, the incorporation of GTR enhanced the thermal stability of the resulting materials.

Abstract Background Hospital services are typically reimbursed using case-mix tools that group patients according to diagnoses and procedures. We recently developed a case-mix tool (i.e., the Queralt system) aimed at supporting clinicians in patient management. In this study, we compared the performance of a broadly used tool (i.e., the APR-DRG) with the Queralt system. Methods Retrospective analysis of all admissions occurred in any of the eight hospitals of the Catalan Institute of Health (i.e., approximately, 30% of all hospitalizations in Catalonia) during 2019. Costs were retrieved from a full cost accounting. Electronic health records were used to calculate the APR-DRG group and the Queralt index, and its different sub-indices for diagnoses (main diagnosis, comorbidities on admission, andcomplications occurred during hospital stay) and procedures (main and secondary procedures). The primary objective was the predictive capacity of the tools; we also investigated efficiency and within-group homogeneity. Results The analysis included 166,837 hospitalization episodes, with a mean cost of € 4,935 (median 2,616; interquartile range 1,011–5,543). The components of the Queralt system had higher efficiency (i.e., the percentage of costs and hospitalizations covered by increasing percentages of groups from each case-mix tool) and lower heterogeneity. The logistic model for predicting costs at pre-stablished thresholds (i.e., 80th, 90th, and 95th percentiles) showed better performance for the Queralt system, particularly when combining diagnoses and procedures (DP): the area under the receiver operating characteristics curve for the 80th, 90th, 95th cost percentiles were 0.904, 0.882, and 0.863 for the APR-DRG, and 0.958, 0.945, and 0.928 for the Queralt DP; the corresponding values of area under the precision-recall curve were 0.522, 0.604, and 0.699 for the APR-DRG, and 0.748, 0.7966, and 0.834 for the Queralt DP. Likewise, the linear model for predicting the actual cost fitted better in the case of the Queralt system. Conclusions The Queralt system, originally developed to predict hospital outcomes, has good performance and efficiency for predicting hospitalization costs.

Over the past decades, health care systems have significantly evolved due to aging populations, chronic diseases, and higher-quality care expectations. Concurrently with the added health care needs, information and communications technology advancements have transformed health care delivery. Technologies such as telemedicine, electronic health records, and mobile health apps promise enhanced accessibility, efficiency, and patient outcomes, leading to more personalized, data-driven care. However, organizational, political, and cultural barriers and the fragmented approach to health information management are challenging the integration of these technologies to effectively support health care delivery. This fragmentation collides with the need for integrated care pathways that focus on holistic health and wellness. Catalonia (northeast Spain), a region of 8 million people with universal health care coverage and a single public health insurer but highly heterogeneous health care service providers, has experienced outstanding digitalization and integration of health information over the past 25 years, when the first transition from paper to digital support occurred. This Viewpoint describes the implementation of health ITs at a system level, discusses the hits and misses encountered in this journey, and frames this regional implementation within the global context. We present the architectures and use trends of the health information platforms over time. This provides insightful information that can be used by other systems worldwide in the never-ending transformation of health care structure and services.

Cette synthèse fait le point des connaissances sur les différentes dimensions de la qualité des produits issus d’élevages en agriculture biologique (AB) vs conventionnelle et sur les méthodes développées pour les authentifier. Les pratiques d’élevage étant variables, en AB comme en agriculture conventionnelle (AC), les résultats sont hétérogènes. Nous soulignons les pratiques à l’origine des différences observées, ce qui permet d’éclairer les conséquences des possibles trajectoires de l’agriculture biologique. De manière générale, la qualité est plus variable pour les produits AB, en lien avec un moindre niveau d’intrants et/ou une plus grande variabilité des conditions d’élevage, ainsi qu’une moindre sélection génétique pour le poulet de chair. Les propriétés améliorées sont la teneur en acides gras polyinsaturés, notamment oméga 3, plus élevée pour le lait de vache et les viandes AB, une réduction du risque de résidus de médicaments et d’apparition de résistances bactériennes aux antimicrobiens. Mais l’accès au plein air et la période d’élevage souvent plus longue augmentent la probabilité et la durée d’exposition des animaux aux éventuels contaminants environnementaux et le risque de leur bioaccumulation dans les produits. Nous identifions des antagonismes entre différentes dimensions de la qualité, notamment pour les viandes ovine et porcine. Les implications de la plus grande variabilité de la qualité des produits AB sur leur acceptabilité par les consommateurs et l’adaptation des procédés de transformation restent peu étudiées. La fiabilité des méthodes d’authentification se heurte à la variabilité des pratiques d’élevage, car elles se fondent sur les différences dans l’alimentation des animaux.

Summary: CombiVacS study has demonstrated a strong immune response of the heterologous ChAdOx1-S/BNT162b2 vaccine combination. The primary outcomes of the study were to assess the humoral immune response against SARS-CoV-2, 28 days after a third dose of a mRNA vaccine, in subjects that received a previous prime-boost scheme with ChAdOx1-S/BNT162b2. Secondary outcomes extended the study to 3 and 6 months. The third vaccine dose of mRNA-1273 in naive participants previously vaccinated with ChAdOx1-S/BNT162b2 regimen reached higher neutralizing antibodies titers against the variants of concern Delta and BA.1 lineage of Omicron compared with those receiving a third dose of BNT162b2 at day 28. These differences between BNT162b2 and mRNA-1273 arms were observed against the ancestral variant G614 at day 90. Suboptimal neutralizing response was observed against BQ.1.1, XBB.1.5/XBB.1.9, and JN.1 in a relevant proportion of individuals 180 days after the third dose, even after asymptomatic Omicron breakthrough infections. EudraCT (2021-001978-37); ClinicalTrials.gov (NCT04860739).

Ensuring food production while enhancing environmental sustainability is a critical challenge in the 21st century. Quantitative descriptions of environmental performance at the farm level are essential for evaluating agricultural production, aligning with climate and biodiversity goals, and facilitating sustainable transitions. However, many existing indicators and proxies rely on costly field-collected data with limited spatial generalization.In this study, we assessed the environmental performance of Uruguayan farms larger than 5 ha using seven synoptic indicators derived from remote sensing. These indicators included the proportion of natural habitats (NatHab), diversity of Ecosystem Functional Types (dEFT), supply of regulating and supporting Ecosystem Services (ESSI), their temporal trends (tESSI), Energy Available for Trophic Network (EATN; 1- Human Appropriation of Net Primary Production), Hydrological Yield (HY), and Soil Conservation (SC).We categorized rural cadastral units from different geomorphological regions into Cropland, Mixed, and Livestock production types. Results showed variations in environmental performance among production systems and regions, with livestock farms generally outperforming and exhibiting less variability. However, cropland farms displayed potential for comparable environmental performance to less intensified areas.Regional disparities were evident, with the Basaltic region demonstrating higher overall performance. Indicators such as NatHabs, dEFT, and EATN exhibited significant variation, reflecting land-use and management practices. HY also showcased notable regional and land-use differences, influenced by soil characteristics and landscape features. SC varied mainly between geomorphological regions. Interestingly, regional patterns differed among indicators, suggesting low redundancy.This study provides valuable insights into environmental performance and its spatial dynamics in the Uruguayan agricultural sector, informing land management and policy decisions. Future studies should engage diverse social actors to develop an environmental performance index for agricultural production, enhancing the sustainability of food production systems.

The central argument of this article is that abduction as a "mode of inference" is a key element in the nature of scientists' science and should consequently be introduced in school science. Abduction generally understood as generation and selection of hypotheses permits to articulate the classical scientific contexts of discovery and justification and provides educational insights into scientific methodology, this being a particularly important issue in science teaching. However, abductive reasoning has been marginally treated in the philosophy of science until relatively recently; accordingly, we deem it important to perform an "archaeology" of the concept that considers C. S. Peirce's seminal contributions. We also choose to review contemporary treatments in order to recognise useful classifications to support more meaningful ways of teaching science and the nature of science. An elucidation of the participation of abductive inferences in knowledge construction seems necessary for us to derive conceptual input for the understanding and design of explanations in school science. Some prospective examples of "school scientific abduction" are discussed in the article through the lens of the results of our theoretical analysis.