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156 results on '"Santana, Jaime M."'

5. Current millennium biotechniques for biomedical research on parasites and host-parasite interactions

Author

Teixeira Antonio RL, Simões-Barbosa Augusto, Faudry Eric, Lozzi Silene P, Argañaraz Enrique R, D'Souza-Ault Marian, and Santana Jaime M

Subjects
genomics, proteomics, transcriptomics, Chagas disease, leishmaniasis, post-genomics, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216
Abstract

The development of biotechnology in the last three decades has generated the feeling that the newest scientific achievements will deliver high standard quality of life through abundance of food and means for successfully combating diseases. Where the new biotechnologies give access to genetic information, there is a common belief that physiological and pathological processes result from subtle modifications of gene expression. Trustfully, modern genetics has produced genetic maps, physical maps and complete nucleotide sequences from 141 viruses, 51 organelles, two eubacteria, one archeon and one eukaryote (Saccharomices cerevisiae). In addition, during the Centennial Commemoration of the Oswaldo Cruz Institute the nearly complete human genome map was proudly announced, whereas the latest Brazilian key stone contribution to science was the publication of the Shillela fastidiosa genomic sequence highlythed on a Nature cover issue. There exists a belief among the populace that further scientific accomplishments will rapidly lead to new drugs and methodological approaches to cure genetic diseases and other incurable ailments. Yet, much evidence has been accumulated, showing that a large information gap exists between the knowledge of genome sequence and our knowledge of genome function. Now that many genome maps are available, people wish to know what are we going to do with them. Certainly, all these scientific accomplishments will shed light on many more secrets of life. Nevertheless, parsimony in the weekly announcements of promising scientific achievements is necessary. We also need many more creative experimental biologists to discover new, as yet un-envisaged biotechnological approaches, and the basic resource needed for carrying out mile stone research necessary for leading us to that "promised land"often proclaimed by the mass media.

Published
2000

6. Mapping Salivary Proteases in Sjögren’s Syndrome Patients Reveals Overexpression of Dipeptidyl Peptidase-4/CD26

Author

Garreto, Laís, primary, Charneau, Sébastien, additional, Mandacaru, Samuel Coelho, additional, Nóbrega, Otávio T., additional, Motta, Flávia N., additional, de Araújo, Carla N., additional, Tonet, Audrey C., additional, Modesto, Flávia M. B., additional, Paula, Lilian M., additional, de Sousa, Marcelo Valle, additional, Santana, Jaime M., additional, Acevedo, Ana Carolina, additional, and Bastos, Izabela M. D., additional

Published
2021
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8. Prolyl Oligopeptidase From Leishmania infantum: Biochemical Characterization and Involvement in Macrophage Infection

Author

Lasse, Camila, primary, Azevedo, Clênia S., additional, de Araújo, Carla N., additional, Motta, Flávia N., additional, Andrade, Milene A., additional, Rocha, Amanda Pereira, additional, Sampaio, Iracyara, additional, Charneau, Sébastien, additional, Gèze, Marc, additional, Grellier, Philippe, additional, Santana, Jaime M., additional, and Bastos, Izabela M. D., additional

Published
2020
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9. Exoproteome profiling of Trypanosoma cruzi during amastigogenesis early stages

Author

Mandacaru, Samuel C., primary, Queiroz, Rayner M. L., additional, Alborghetti, Marcos R., additional, de Oliveira, Lucas S., additional, de Lima, Consuelo M. R., additional, Bastos, Izabela M. D., additional, Santana, Jaime M., additional, Roepstorff, Peter, additional, Ricart, Carlos André O., additional, and Charneau, Sébastien, additional

Published
2019
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10. Advances in nanocarriers as drug delivery systems in Chagas disease

Author

Quijia Quezada,Christian, Azevedo,Clênia S, Charneau,Sébastien, Santana,Jaime M, Chorilli,Marlus, Carneiro,Marcella B, and Bastos,Izabela Marques Dourado

Subjects
International Journal of Nanomedicine
Abstract

Christian Quijia Quezada,1,2 Clênia S Azevedo,1 Sébastien Charneau,3 Jaime M Santana,1 Marlus Chorilli,2 Marcella B Carneiro,4 Izabela Marques Dourado Bastos11Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasília, Brazil; 2Department of Drugs and Medicines, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 3Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasília, Brazil; 4Electron Microscopy Laboratory, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasília, BrazilAbstract: Chagas disease is one of the most important public health problems in Latin America due to its high mortality and morbidity levels. There is no effective treatment for this disease since drugs are usually toxic with low bioavailability. Serious efforts to achieve disease control and eventual eradication have been unsuccessful to date, emphasizing the need for rapid diagnosis, drug development, and a reliable vaccine. Novel systems for drug and vaccine administration based on nanocarriers represent a promising avenue for Chagas disease treatment. Nanoparticulate systems can reduce toxicity, and increase the efficacy and bioavailability of active compounds by prolonging release, and therefore improve the therapeutic index. Moreover, nanoparticles are able to interact with the host’s immune system, modulating the immune response to favour the elimination of pathogenic microorganisms. In addition, new advances in diagnostic assays, such as nanobiosensors, are beneficial in that they enable precise identification of the pathogen. In this review, we provide an overview of the strategies and nanocarrier-based delivery systems for antichagasic agents, such as liposomes, micelles, nanoemulsions, polymeric and non-polymeric nanoparticles. We address recent progress, with a particular focus on the advances of nanovaccines and nanodiagnostics, exploring new perspectives on Chagas disease treatment.Keywords: delivery systems, nanobiosensors, nanodiagnostics, nanoparticle systems, nanovaccine

Published
2019

12. A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins

Author

Soares Célia MA, Bailão Alexandre M, Borges Clayton L, Pereira Maristela, Santana Jaime M, Salem-Izacc Sílvia M, and Parente Juliana A

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Paracoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi. Results A cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2. Conclusions A secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.

Published
2010
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13. Trypanosoma cruzi alkaline 2-DE: Optimization and application to comparative proteome analysis of flagellate life stages

Author

Santana Jaime M, Teixeira Antonio RL, Guércio Rafael AP, Paba Jaime, Charneau Sébastien, Magalhães Adriana D, Sousa Marcelo V, and Ricart Carlos AO

Subjects
Cytology, QH573-671
Abstract

Abstract Background Trypanosoma cruzi, a flagellate protozoan, is the etiological agent of Chagas disease, a chronic illness that causes irreversible damage to heart and digestive tract in humans. Previous 2-DE analyses of T. cruzi proteome have not focused on basic proteins, possibly because of inherent difficulties for optimizing 2-DE in the alkaline pH range. However, T. cruzi wide pH range 2-DE gels have shown few visible spots in the alkaline region, indicating that the parasite either did not have an appreciable amount of alkaline proteins or that these proteins were underrepresented in the 2-DE gels. Results Different IEF conditions using 6–11 pH gradient strips were tested for separation of T. cruzi alkaline proteins. The optimized methodology described here was performed using anodic "paper bridge" sample loading supplemented by increased concentration of DTT and Triton X-100 on Multiphor II (GE Healthcare) equipment and an electrode pad embedded in DTT- containing solution near the cathode in order to avoid depletion of reducing agent during IEF. Landmark proteins were identified by peptide mass fingerprinting allowing the production of an epimastigote 2-DE map. Most identified proteins corresponded to metabolic enzymes, especially those related to amino acid metabolism. The optimized 2-DE protocol was applied in combination with the "two-in-one gel" method to verify the relative expression of the identified proteins between samples from epimastigote and trypomastigote life stages. Conclusion High resolution 2-DE gels of T. cruzi life forms were achieved using the optimized methodology and a partial epimastigote alkaline 2-DE map was built. Among 700 protein spots detected, 422 were alkaline with a pI above 7.0. The "two-in-one gel" method simplified the comparative analysis between T. cruzi life stages since it minimized variations in spot migration and silver-stained spot volumes. The comparative data were in agreement with biological traits of T. cruzi life forms and also corroborated previous T. cruzi proteomic studies. For instance, enzymes related to amino acid metabolism and dehydrogenases were more abundant in epimastigote 2-DE gel whilst trans-sialidase and a paraflagellar protein were found specifically in the trypomastigote 2-DE profile.

Published
2008
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19. In vitro investigation of Brazilian Cerrado plant extract activity against Plasmodium falciparum , Trypanosoma cruzi and T. brucei gambiense

Author

Charneau, Sébastien, De Mesquita, Mariana Laundry, Bastos, Izabela Marques, Santana, Jaime M, De Paula, J. E., Grellier, Philippe, Espindola, Laila S, Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), and Grellier, Philippe

Subjects
[SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV]Life Sciences [q-bio], [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

23. A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease

Author

Santiago, Paula Beatriz, primary, Assumpção, Teresa C. F., additional, Araújo, Carla Nunes de, additional, Bastos, Izabela Marques Dourado, additional, Neves, David, additional, Silva, Ionizete Garcia da, additional, Charneau, Sébastien, additional, Queiroz, Rayner Myr L., additional, Raiol, Tainá, additional, Oliveira, João Victor de Araújo, additional, Sousa, Marcelo Valle de, additional, Calvo, Eric, additional, Ribeiro, José M. C., additional, and Santana, Jaime M., additional

Published
2016
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32. Salivary Antigen-5/CAP Family Members Are Cu2+-dependent Antioxidant Enzymes That Scavenge O2⨪ and Inhibit Collagen-induced Platelet Aggregation and Neutrophil Oxidative Burst

Author

Assumpção, Teresa C.F., primary, Ma, Dongying, additional, Schwarz, Alexandra, additional, Reiter, Karine, additional, Santana, Jaime M., additional, Andersen, John F., additional, Ribeiro, José M.C., additional, Nardone, Glenn, additional, Yu, Lee L., additional, and Francischetti, Ivo M.B., additional

Published
2013
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34. Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.

Author

de Athayde Couto Falcão, Sarah, Jaramillo, Tatiana M. G., Ferreira, Luciana G., Bernardes, Daniela M., Santana, Jaime M., and Favali, Cecília B. F.

Subjects
VISCERAL leishmaniasis, CD86 antigen, IMMUNE response
Abstract

Visceral leishmaniasis is a severe form of the disease, caused by Leishmania infantum in the New World. Patients present an anergic immune response that favors parasite establishment and spreading through tissues like bone marrow and liver. On the other hand, Leishmania braziliensis causes localized cutaneous lesions, which can be self-healing in some individuals. Interactions between host and parasite are essential to understand disease pathogenesis and progression. In this context, dendritic cells (DCs) act as essential bridges that connect innate and adaptive immune responses. In this way, the aim of this study was to compare the effects of these two Leishmania species, in some aspects of human DCs' biology for better understanding of the evasion mechanisms of Leishmania from host innate immune response. To do so, DCs were obtained from monocytes from whole peripheral blood of healthy volunteer donors and from those infected with L. infantum or L. braziliensis for 24 h. We observed similar rates of infection (around 40%) as well as parasite burden for both Leishmania species. Concerning surface molecules, we observed that both parasites induced CD86 expression when DCs were infected for 24 h. On the other hand, we detected a lower surface expression of CD209 in the presence of both L. braziliensis and L. infantum, but only the last one promoted the survival of DCs after 24 h. Therefore, DCs infected by both Leishmania species showed a higher expression of CD86 and a decrease of CD209 expression, suggesting that both enter DCs through CD209 molecule. However, only L. infantum had the ability to inhibit DC apoptotic death, as an evasion mechanism that enables its spreading to organs like bone marrow and liver. Lastly, L. braziliensis was more silent parasite, once it did not inhibit DC apoptosis in our in vitro model. [ABSTRACT FROM AUTHOR]

Published
2016
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35. The Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zone

Author

Galetović, Alexandra, primary, Souza, Renata T., additional, Santos, Marcia R. M., additional, Cordero, Esteban M., additional, Bastos, Izabela M. D., additional, Santana, Jaime M., additional, Ruiz, Jeronimo C., additional, Lima, Fabio M., additional, Marini, Marjorie M., additional, Mortara, Renato A., additional, and da Silveira, José Franco, additional

Published
2011
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36. The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases

Author

Cadavid-Restrepo, Gloria, primary, Gastardelo, Thiago S, additional, Faudry, Eric, additional, de Almeida, Hugo, additional, Bastos, Izabela MD, additional, Negreiros, Raquel S, additional, Lima, Meire M, additional, Assumpção, Teresa C, additional, Almeida, Keyla C, additional, Ragno, Michel, additional, Ebel, Christine, additional, Ribeiro, Bergmann M, additional, Felix, Carlos R, additional, and Santana, Jaime M, additional

Published
2011
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37. Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima

Author

Assumpção, Teresa C. F., primary, Charneau, Sébastien, additional, Santiago, Paula B. M., additional, Francischetti, Ivo M. B., additional, Meng, Zhaojing, additional, Araújo, Carla N., additional, Pham, Van M., additional, Queiroz, Rayner M. L., additional, Nery de Castro, Cleudson, additional, André Ricart, Carlos, additional, Santana, Jaime M., additional, and Ribeiro, José M. C., additional

Published
2011
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43. Inserção de DNA de Trypanosoma cruzi no genoma de célula hospedeira de mamífero por meio de infecção

Author

Teixeira, Antonio R.L., Lacava, Zulmira, Santana, Jaime M., and Luna, Helena

Subjects
Transxenogene, Macrophage, Trypanosoma cruzi, parasitic diseases, Macrófago
Abstract

Observamos a cromatina deformas amastigotas de T. cruzi associada a cromossomos de macrófagos metafásicos obtidos em diversos períodos da infecção aguda. O estudo imunocitogenético demonstrou que o material genético inserido naqueles cromossomos era produto do T. cruzi. Pelo teste de hibridizaçâo in situ com sonda biotinilada de DNA de T. cruzi, foi confirmada a inserção de DNA do protozoário nos cromossomos murinos. A inserção seletiva de ³H-DNA do protozoário em alguns cromossomos sugere que podem ocorrer rearranjos transxenogénicos em infecções de mamíferos pelo T. cruzi. Trypanosoma cruzi chromatin was observed in macrophage chromosome spreads obtained at different periods of infections in BALB/c mice. Immunofluorescent studies showed that genetic materials associated with the chromosomes were T. cruzi products. In situ hybridization showed the protozoon DNA insertion in the host cell genome. In addition, selective ³H-DNA insertion in chromosomes 3, 6 and 11 was observed, which suggested that transxenogene rearrangement may take place in T. cruzi infections of mammals.

Published
1991

46. Molecular, functional and structural properties of the prolyl oligopeptidase of Trypanosoma cruzi (POP Tc80), which is required for parasite entry into mammalian cells

Author

BASTOS, Izabela M. D., primary, GRELLIER, Philippe, additional, MARTINS, Natalia F., additional, CADAVID-RESTREPO, Gloria, additional, de SOUZA-AULT, Marian R., additional, AUGUSTYNS, Koen, additional, TEIXEIRA, Antonio R. L., additional, SCHRÉVEL, Joseph, additional, MAIGRET, Bernard, additional, da SILVEIRA, José F., additional, and SANTANA, Jaime M., additional

Published
2005
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50. Triatoma infestans Apyrases Belong to the 5′-Nucleotidase Family

Author

Faudry, Eric, primary, Lozzi, Silene P., additional, Santana, Jaime M., additional, D'Souza-Ault, Marian, additional, Kieffer, Sylvie, additional, Felix, Carlos R., additional, Ricart, Carlos A.O., additional, Sousa, Marcelo V., additional, Vernet, Thierry, additional, and Teixeira, Antonio R.L., additional

Published
2004
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